LOCUS       MKC175_HBA1(synEPOR)_Ad 5943 bp ds-DNA     circular     10-JAN-2025
DEFINITION  .
KEYWORDS    "creator:Trial User" "marker:Right_AAV2 ITR, AmpR, Amplicon for
            qPCR, Amplicon for qPCR, HBA1_5'UTR, synEPOR, HBA1_3'UTR"
FEATURES             Location/Qualifiers
     LTR             1..130
                     /label="Left-ITR_AAV"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    68..130
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    133..1078
                     /label="LHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1013..1078
                     /label="HBA1_5'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     sig_peptide     1079..1159
                     /label="Signal peptide - IL6"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="codon_start: 1 transl_table: 1 translation: MNSFSTSAFGPVAFSLGLLLVLPAAFP,AP"
     misc_feature    1079..2095
                     /label="synEPOR"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     sig_peptide     1160..1165
                     /label="Signal peptide - IL6"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="codon_start: 1 transl_table: 1 translation: MNSFSTSAFGPVAFSLGLLLVLPAAFP,AP"
     misc_feature    1166..1180
                     /label="GGGGS_linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1181..1189
                     /label="FKBP-V"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: FKBP-V"
     CDS             1190..1510
                     /label="FKBP-V"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="label: FKBP-V"
     misc_feature    1511..1525
                     /label="divGGGGS_linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1526..2092
                     /label="tEPOR_cDNA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2093..2095
                     /label="STOP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     polyA_signal    2096..2320
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
                     /note="note: bovine growth hormone polyadenylation signal"
     misc_feature    2321..2418
                     /label="HBA1_3'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2321..3199
                     /label="RHA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3206..3268
                     /label="Amplicon for qPCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   3206..3346
                     /label="Right_AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      3421..3876
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        4158..4262
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             4263..4331
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             4332..5123
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      5294..5882
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCgctccagc cggttccagc tattgctttg tttacctgtt taaccagtat
      181 ttacctagca agtcttccat cagatagcat ttggagagct gggggtgtca cagtgaacca
      241 cgacctctag gccagtggga gagtcagtca cacaaactgt gagtccatga cttggggctt
      301 agccCgcacc caccacccca cgcgccaccc cacaaccccg ggtagaggag tctgaatctg
      361 gagccgcccT cagcccagcc ccCtgctttt tgcgtcctgg tgtttattcc ttcccggtgc
      421 cCgtcactca agcacactGg tgactatcgc cagagggaaa gggagctgca ggaagcgagg
      481 ctggagagca ggaggggctc tgcgcagaaa ttcttttgag ttcctatggg ccagggcgtc
      541 cgggtgcgcg cattcctctc cgccccagga ttgggcgaag cctcccggct cgcactcgct
      601 cgcccgtgtg ttccccgatc ccgctggagt cgatgcgcgt ccagcgcgtg ccaggccggg
      661 gcgggggtgc gggctgactt tctccctcgc tagggacgct ccggcgcccg aaaggaaagg
      721 gtggcgctgc gctccggggt gcacgagccg acagcgcccg accccaacgg gccggccccg
      781 ccagcgccgc taccgccctg cccccgggcg agcgggatgg gcgggagtgg agtggcgggt
      841 ggagggtgga gacgtcctgg cccccgcccc gcgtgcaccc ccaggggagg ccgagcccgc
      901 cgcccggccc cgcgcaggcc ccgcccggga ctcccctgcg gtccaggccg cgccccgggc
      961 tccgcgccag ccaatgagcg ccgcccggcc gggcgtgccc ccgcgcccca agcataaacc
     1021 ctggcgcgct cgcggcccgg cactcttctg gtccccacag actcagagag aacccaccAT
     1081 GAACTCCTTC TCCACAAGCG CCTTCGGTCC AGTTGCCTTC TCCCTGGGGC TGCTCCTGGT
     1141 GTTGCCTGCT GCCTTCCCTG CCCCAGGcGG cGGcGGaAGc ATGCTTGAGG GAGTGCAGGT
     1201 GGAAACCATC TCCCCAGGAG ACGGGCGCAC CTTCCCCAAG CGCGGCCAGA CCTGCGTGGT
     1261 GCACTACACC GGGATGCTTG AAGATGGAAA GAAAGTTGAT TCCTCCCGGG ACAGAAACAA
     1321 GCCCTTTAAG TTTATGCTAG GCAAGCAGGA GGTGATCCGA GGCTGGGAAG AAGGGGTTGC
     1381 CCAGATGAGT GTGGGTCAGA GAGCCAAACT GACTATATCT CCAGATTATG CCTATGGTGC
     1441 CACTGGGCAC CCAGGCATCA TCCCACCACA TGCCACTCTC GTCTTCGATG TGGAGCTTCT
     1501 AAAACTGGAA GGAGGAGGAG GTAGTCCCCT CATCCTGACG CTCTCCCTCA TCCTCGTGGT
     1561 CATCCTGGTG CTGCTGACCG TGCTCGCGCT GCTCTCCCAC CGCCGGGCTC TGAAGCAGAA
     1621 GATCTGGCCT GGCATCCCGA GCCCAGAGAG CGAGTTTGAA GGCCTCTTCA CCACCCACAA
     1681 GGGTAACTTC CAGCTGTGGC TGTACCAGAA TGATGGCTGC CTGTGGTGGA GCCCCTGCAC
     1741 CCCCTTCACG GAGGACCCAC CTGCTTCCCT GGAAGTCCTC TCAGAGCGCT GCTGGGGGAC
     1801 GATGCAGGCA GTGGAGCCGG GGACAGATGA TGAGGGCCCC CTGCTGGAGC CAGTGGGCAG
     1861 TGAGCATGCC CAGGATACCT ATCTGGTGCT GGACAAATGG TTGCTGCCCC GGAACCCGCC
     1921 CAGTGAGGAC CTCCCAGGGC CTGGTGGCAG TGTGGACATA GTGGCCATGG ATGAAGGCTC
     1981 AGAAGCATCC TCCTGCTCAT CTGCTTTGGC CTCGAAGCCC AGCCCAGAGG GAGCCTCTGC
     2041 TGCCAGCTTT GAGTACACTA TCCTGGACCC CAGCTCCCAG CTCTTGCGTC CAtaactgtg
     2101 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2161 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2221 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2281 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg tggccatgct tcttgcccct
     2341 tgggcctccc cccagcccct cctccccttc ctgcacccgt acccccgtgg tctttgaata
     2401 aagtctgagt gggcggcagc ctgtgtgtgc ctgagttttt tccctcagca aacgtgccag
     2461 gcatgggTgt ggacagcagc tgggacacac atggctagaa cctctctgca gctggatagg
     2521 gtaggaaaag gcaggggcgg gaggagggga tggaggaggg aaagtggagc caccgcgaag
     2581 tccagctgga aaaacgctgg accctagagt gctttgagga tgcatttgct ctttcccgag
     2641 ttttattccc agacttttca gattcaatgc aggtttgctg aaataatgaa tttatccatc
     2701 tttacgtttc tgggcactct Tgtgccaaga actggctggc tttctgcctg ggacgtcact
     2761 ggtttcccag aggtcctccc acatatgggt ggtgggtagg tcagagaagt cccactccag
     2821 catggctgca ttgatccccc atcgttccca ctagtctccg taaaacctcc cagatacagg
     2881 cacagtctag atgaaatcag gggtgcgggg tgcaactgca ggccccaggc aattcaatag
     2941 gggctctact ttcaccccca ggtcacccca gaatgctcac acaccagaca ctgacgccct
     3001 ggggctgtca agatcaggcg tttgtctctg ggcccagctc agggcccagc tcagcaccca
     3061 ctcagctccc ctgaggctgg ggagcctgtc ccattgcgac tggagaggag agcggggcca
     3121 cagaggcctg gctagaaggt cccttctccc tggtgtgtgt tttctctctg ctgagcaggc
     3181 ttgcagtgcc tggggtacaG GCCGCAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCtTCT
     3241 CTGCGCGCTC GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT
     3301 GCCCGGGCGG CCTCAGTGAG CGAGCGAGCG CGCAGCTGCC TGCAGGGGCG CCTGATGCGG
     3361 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TACGTCAAAG CAACCATAGT
     3421 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
     3481 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
     3541 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA
     3601 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC
     3661 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG
     3721 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGGCTATTCT TTTGATTTAT
     3781 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA
     3841 ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTTATG GTGCACTCTC AGTACAATCT
     3901 GCTCTGATGC CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT
     3961 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT
     4021 GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA
     4081 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA
     4141 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA
     4201 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA
     4261 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC
     4321 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG
     4381 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC
     4441 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT
     4501 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT
     4561 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT
     4621 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA
     4681 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC
     4741 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA
     4801 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG
     4861 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC
     4921 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT
     4981 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT
     5041 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG
     5101 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG
     5161 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
     5221 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
     5281 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     5341 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     5401 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT
     5461 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     5521 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
     5581 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
     5641 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
     5701 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
     5761 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
     5821 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
     5881 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
     5941 TGT
//