LOCUS       hSyn-hCPT2-HALO       11244 bp DNA     circular SYN 03-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 3, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
COMMENT     Sequence Label: hSyn-hCPT2-HALO
FEATURES             Location/Qualifiers
     source          1..11244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2539..2986
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3015..3066
                     /label=CPT2 XhoI HiFi F
                     /note="color: black; sequence: 
                     cagatatccatcacactggcggccgctcgaggccaccATGGTGCCCCGCCTG"
     regulatory      3046..3051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             3052..5025
                     /codon_start=1
                     /label=Human-CPT2
                     /note="color: #993366"
                     /translation="MVPRLLLRAWPRGPAVGPGAPSRPLSAGSGPGQYLQRSIVPTMHY
                     QDSLPRLPIPKLEDTIRRYLSAQKPLLNDGQFRKTEQFCKSFENGIGKELHEQLVALDK
                     QNKHTSYISGPWFDMYLSARDSVVLNFNPFMAFNPDPKSEYNDQLTRATNMTVSAIRFL
                     KTLRAGLLEPEVFHLNPAKSDTITFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFN
                     STRLPKPSRDELFTDDKARHLLVLRKGNFYIFDVLDQDGNIVSPSEIQAHLKYILSDSS
                     PAPEFPLAYLTSENRDIWAELRQKLMSSGNEESLRKVDSAVFCLCLDDFPIKDLVHLSH
                     NMLHGDGTNRWFDKSFNLIIAKDGSTAVHFEHSWGDGVAVLRFFNEVFKDSTQTPAVTP
                     QSQPATTDSTVTVQKLNFELTDALKTGITAAKEKFDATMKTLTIDCVQFQRGGKEFLKK
                     QKLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVYTKRCSEAFV
                     REPSRHSAGELQQMMVECSKYHGQLTKEAAMGQGFDRHLFALRHLAAAKGIILPELYLD
                     PAYGQINHNVLSTSTLSSPAVNLGGFAPVVSDGFGVGYAVHDNWIGCNVSSYPGRNARE
                     FLQCVEKALEDMFDALEGKSIKS"
     primer_bind     complement(5000..5056)
                     /label=CPT2 XhoI HiFi R
                     /note="color: black; sequence: 
                     CGGTGCCGATTTCTCTAGATGCATGCTCGAGACTTTTGATGGATTTGCCTTCTAAGG"
     CDS             5044..5925
                     /codon_start=1
                     /label=HALO
                     /note="color: #ff0000"
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     primer_bind     complement(5044..5061)
                     /label=HALO N R
                     /note="color: black; sequence: GAAGCCGGTGCCGATTTC"
     misc_feature    6050..6638
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(6103..6123)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(6521..6532)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 6520"
                     /translation="IEGR"
     LTR             7045..7225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(7251..7268)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    7257..7481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7527..7955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(7614..7633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     7824..7845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(7964..7984)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        7969..8299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8150..8285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     8212..8231
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     promoter        8347..8394
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8413..8787
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8917..9038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(8954..8973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     9008..9027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(9087..9103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(9087..9103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(9100..9122)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    9111..9127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(9135..9141,9142..9159,9160..9165))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9135 .. 9141 / #ffffff / -10
                        2: 9142 .. 9159 / #ffffff
                        3: 9160 .. 9165 / #ffffff / -35"
     protein_bind    9180..9201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(9318..9335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(9489..10077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(9569..9588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(join(10248..11039,11040..11108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10248 .. 11039 / #ccffcc
                        2: 11040 .. 11108 / #ccffcc / signal sequence
                     Cleavage site after base 11039"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10871..10890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(11109..11213)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgctagactg
     2521 cagagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2581 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2641 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2701 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2761 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     2821 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     2881 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     2941 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga tctagacgat
     3001 aagggcgaat tctgcagata tccatcacac tggcggccgc tcgaggccac catggtgccc
     3061 cgcctgctgc tgcgcgcctg gccccggggc cccgcggttg gtccgggagc ccccagtcgg
     3121 cccctcagcg ccggctccgg gcccggccag tacctgcagc gcagcatcgt gcccaccatg
     3181 cactaccagg acagcctgcc caggctgcct attcccaaac ttgaagacac cattaggaga
     3241 tacctcagtg cacagaagcc tctcttgaat gatggccagt tcaggaaaac agaacaattt
     3301 tgcaagagtt ttgaaaatgg gattggaaaa gaactgcatg agcagctggt tgctctggac
     3361 aaacagaata aacatacaag ctacatttcg ggaccctggt ttgatatgta cctatctgct
     3421 cgagactccg ttgttctgaa ctttaatcca tttatggctt tcaatcctga cccaaaatct
     3481 gagtataatg accagctcac ccgggcaacc aacatgactg tttctgccat ccggtttctg
     3541 aagacactcc gggctggcct tctggagcca gaagtgttcc acttgaaccc tgcaaaaagt
     3601 gacactatca ccttcaagag actcatacgc tttgtgcctt cctctctgtc ctggtatggg
     3661 gcctacctgg tcaatgcgta tcccctggat atgtcccagt attttcggct tttcaactca
     3721 actcgtttac ccaaacccag tcgggatgaa ctcttcactg atgacaaggc cagacacctc
     3781 ctggtcctaa ggaaaggaaa tttttatatc tttgatgtcc tggatcaaga tgggaacatt
     3841 gtgagcccct cggaaatcca ggcacatctg aagtacattc tctcagacag cagccccgcc
     3901 cccgagtttc ccctggcata cctgaccagt gagaaccgag acatctgggc agagctcagg
     3961 cagaagctga tgagtagtgg caatgaggag agcctgagga aagtggactc ggcagtgttc
     4021 tgtctctgcc tagatgactt ccccattaag gaccttgtcc acttgtccca caatatgctg
     4081 catggggatg gcacaaaccg ctggtttgat aaatccttta acctcattat cgccaaggat
     4141 ggctctactg ccgtccactt tgagcactct tggggtgatg gtgtggcagt gctcagattt
     4201 tttaatgaag tatttaaaga cagcactcag acccctgccg tcactccaca gagccagcca
     4261 gctaccactg actctactgt cacggtgcag aaactcaact tcgagctgac tgatgcctta
     4321 aagactggca tcacagctgc taaggaaaag tttgatgcca ccatgaaaac cctcactatt
     4381 gactgcgtcc agtttcagag aggaggcaaa gaattcctga agaagcaaaa gctgagccct
     4441 gacgcagttg cccagctggc attccagatg gccttcctgc ggcagtacgg gcagacagtg
     4501 gccacctacg agtcctgtag cactgccgca ttcaagcacg gccgcactga gaccatccgc
     4561 ccggcctccg tctatacaaa gaggtgctct gaggcctttg tcagggagcc ctccaggcac
     4621 agtgctggtg agcttcagca gatgatggtt gagtgctcca agtaccatgg ccagctgacc
     4681 aaagaagcag caatgggcca gggctttgac cgacacttgt ttgctctgcg gcatctggca
     4741 gcagccaaag ggatcatctt gcctgagctc tacctggacc ctgcatacgg gcagataaac
     4801 cacaatgtcc tgtccacgag cacactgagc agcccagcag tgaaccttgg gggctttgcc
     4861 cctgtggtct ctgatggctt tggtgttggg tatgctgttc atgacaactg gataggctgc
     4921 aatgtctctt cctacccagg ccgcaatgcc cgggagtttc tccaatgtgt ggagaaggcc
     4981 ttagaagaca tgtttgatgc cttagaaggc aaatccatca aaagtctcga gcatgcatct
     5041 agagaaatcg gcaccggctt ccctttcgac ccccactacg tggaagtgct gggcgagaga
     5101 atgcactatg tggacgtggg ccctagggac ggaacccccg tgctgtttct gcacggcaac
     5161 cccaccagca gctacgtgtg gcggaacatc atcccccacg tggcccccac ccacagatgt
     5221 atcgcccctg acctgatcgg catgggcaag agcgacaagc ccgacctggg ctacttcttc
     5281 gacgaccacg tgcggttcat ggacgccttc atcgaggccc tgggcctgga agaggtggtg
     5341 ctcgtgatcc acgattgggg ctctgccctg ggcttccact gggccaagag aaaccccgag
     5401 agagtgaagg gaatcgcctt catggaattc atcagaccca tccccacctg ggacgagtgg
     5461 cccgagttcg ccagagagac attccaggcc ttcagaacca ccgacgtggg cagaaagctg
     5521 atcatcgacc agaacgtgtt catcgaaggc accctgccta tgggcgtcgt gcggcctctg
     5581 accgaggtgg aaatggacca ctacagagag ccctttctga accccgtgga ccgcgaacct
     5641 ctgtggcggt tccctaacga gctgcctatc gctggcgagc ccgccaacat tgtggccctg
     5701 gtggaagagt acatggactg gctgcaccag agccccgtgc ccaagctgct gttttgggga
     5761 acacccggcg tgctgatccc tcctgctgag gctgctagac tggccaagag cctgcccaac
     5821 tgcaaggccg tggatatcgg ccctggcctg aacctgctgc aggaagataa ccccgatctg
     5881 atcggctccg agatcgcccg gtggctgagc accctggaaa tcagctaggt ttaaacctgg
     5941 aaaaacatgg agcaatcaca agtagcaaca cagcagctac caatgctgct tgtgcctggc
     6001 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcagaca atcaacctct
     6061 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6121 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6181 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6241 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6301 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6361 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6421 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6481 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6541 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6601 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct
     6661 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6721 ggactggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     6781 cacacacaag gctacttccc tgattgacag aactacacac cagggccagg ggtcagatat
     6841 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa gatagaagag
     6901 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     6961 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     7021 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     7081 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7141 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7201 tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc cgctgatcag cctcgactgt
     7261 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7321 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7381 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7441 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7501 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7561 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7621 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7681 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7741 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7801 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7861 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa
     7921 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7981 gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa
     8041 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8101 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8221 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     8281 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     8341 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     8401 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     8461 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     8521 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     8581 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     8641 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     8701 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     8761 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     8821 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8881 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8941 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9001 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     9061 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     9121 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9181 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9241 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9661 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     9721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9901 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    10021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    10081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11221 ccccgaaaag tgccacctga cgtc
//
