LOCUS       M237_PHB1_mCherr        5537 bp    DNA        circular     13-JAN-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     AssembledSeq
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     rep_origin      1019..1647
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      4206..4646
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        4945..5141
                     /locus_tag="SV40 early promoter"
                     /label="SV40 early promoter"
                     /ApEinfo_label="SV40 early promoter"
                     /ApEinfo_fwdcolor="#6495ED"
                     /ApEinfo_revcolor="#6495ED"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             3238..3942
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#FF0000"
                     /ApEinfo_revcolor="#FF0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     Promoter        4713..4741
                     /locus_tag="Amp prom"
                     /label="Amp prom"
                     /ApEinfo_label="Amp prom"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4992..5069
                     /locus_tag="SV40 ORI"
                     /label="SV40 ORI"
                     /ApEinfo_label="SV40 ORI"
                     /ApEinfo_fwdcolor="#4682B4"
                     /ApEinfo_revcolor="#4682B4"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(3990..4009)
                     /locus_tag="pEGFP_Rev"
                     /label="pEGFP_Rev"
                     /ApEinfo_label="pEGFP_Rev"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3382..3401
                     /locus_tag="mCherry seq F"
                     /label="mCherry seq F"
                     /ApEinfo_label="mCherry seq F"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(3864..3883)
                     /locus_tag="mCherry seq R"
                     /label="mCherry seq R"
                     /ApEinfo_label="mCherry seq R"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2262..2282
                     /locus_tag="pCMV F"
                     /label="pCMV F"
                     /ApEinfo_label="pCMV F"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(3429..3448)
                     /locus_tag="pUAST AURA mCherry seq R"
                     /label="pUAST AURA mCherry seq R"
                     /ApEinfo_label="pUAST AURA mCherry seq R"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2362..3177
                     /locus_tag="PHB1"
                     /label="PHB1"
                     /ApEinfo_label="PHB1"
                     /ApEinfo_fwdcolor="#FFFF00"
                     /ApEinfo_revcolor="#FFFF00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
       61 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      121 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
      181 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      241 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      301 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      361 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
      421 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
      481 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      541 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
      601 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
      661 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
      721 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
      781 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
      841 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
      901 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      961 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     1201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1681 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     1741 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1801 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1861 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1921 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1981 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2041 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2101 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2161 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2221 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2281 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     2341 ctaccggact cagatctcga gatggctgcc aaagtgtttg agtccattgg caagtttggc
     2401 ctggccttag ctgttgcagg aggcgtggtg aactctgcct tatataatgt ggatgctggg
     2461 cacagagctg tcatctttga ccgattccgt ggagtgcagg acattgtggt aggggaaggg
     2521 actcattttc tcatcccgtg ggtacagaaa ccaattatct ttgactgccg ttctcgacca
     2581 cgtaatgtgc cagtcatcac tggtagcaaa gatttacaga atgtcaacat cacactgcgc
     2641 atcctcttcc ggcctgtcgc cagccagctt cctcgcatct tcaccagcat cggagaggac
     2701 tatgatgagc gtgtgctgcc gtccatcaca actgagatcc tcaagtcagt ggtggctcgc
     2761 tttgatgctg gagaactaat cacccagaga gagctggtct ccaggcaggt gagcgacgac
     2821 cttacagagc gagccgccac ctttgggctc atcctggatg acgtgtcctt gacacatctg
     2881 accttcggga aggagttcac agaagcggtg gaagccaaac aggtggctca gcaggaagca
     2941 gagagggcca gatttgtggt ggaaaaggct gagcaacaga aaaaggcggc catcatctct
     3001 gctgagggcg actccaaggc agctgagctg attgccaact cactggccac tgcaggggat
     3061 ggcctgatcg agctgcgcaa gctggaagct gcagaggaca tcgcgtacca gctctcacgc
     3121 tctcggaaca tcacctacct gccagcgggg cagtccgtgc tcctccagct gccccagaag
     3181 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     3241 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     3301 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     3361 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     3421 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     3481 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     3541 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     3601 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     3661 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     3721 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     3781 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     3841 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     3901 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     3961 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     4021 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     4201 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4261 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4321 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4381 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4441 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4501 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4561 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4621 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     4681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     4801 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4861 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4921 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4981 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5041 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5101 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     5161 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5221 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5281 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5341 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5401 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5461 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5521 atggctgatg caatgcg    
//
