LOCUS       pCV019                  4061 bp ds-DNA     circular     14-DEC-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5..36
                     /label="Himar1 IR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    57..192
                     /label="cepA promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    193..816
                     /label="catP"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    817..836
                     /label="RBtn-seq Fwd"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    837..870
                     /label="Barcode entry site"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    871..895
                     /label="RBtn-seq Rev"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    885..916
                     /label="Himar1 IR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      935..1098
                     /label="P1E6 promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     regulatory      1099..1131
                     /label="RBS8"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1132..2178
                     /label="Mariner transposase C9 mutant"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     rep_origin      2211..2599
                     /label="R6K gamma ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     oriT            2714..2823
                     /label="RP4 oriT"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2987..3847)
                     /label="AmpR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        3848..3952
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CCTAACAGGT TGGATGATAA GTCCCCGGTC TTCGTAGATA AAACGAAAGG CTCAGTAAAG
       61 AGTTAAGGAA AGTGAAGCAT CTTCGATGCT GGAGTGGGAT ATACTAAATT ACCTAAAAAA
      121 GTGGCGCACA AATTTGCGCG CCACAATTAT TATTCATACC TTTGTGGACC GTATTACAAA
      181 GAACCCAATC ATATGGTATT TGAAAAAATT GATAAAAATA GTTGGAACAG AAAAGAGTAT
      241 TTTGACCACT ACTTTGCAAG TGTACCTTGT ACATACAGCA TGACCGTTAA AGTGGATATC
      301 ACACAAATAA AGGAAAAGGG AATGAAACTA TATCCTGCAA TGCTTTATTA TATTGCAATG
      361 ATTGTAAACC GCCATTCAGA GTTTAGGACG GCAATCAATC AAGATGGTGA ATTGGGGATA
      421 TATGATGAGA TGATACCAAG CTATACAATA TTTCACAATG ATACTGAAAC ATTTTCCAGC
      481 CTTTGGACTG AGTGTAAGTC TGACTTTAAA TCATTTTTAG CAGATTATGA AAGTGATACG
      541 CAACGGTATG GAAACAATCA TAGAATGGAA GGAAAGCCAA ATGCTCCGGA AAACATTTTT
      601 AATGTATCTA TGATACCGTG GTCAACCTTC GATGGCTTTA ATCTGAATTT GCAGAAAGGA
      661 TATGATTATT TGATTCCTAT TTTTACTATG GGGAAATATT ATAAAGAAGA TAACAAAATT
      721 ATACTTCCTT TGGCAATTCA AGTTCATCAC GCAGTATGTG ACGGATTTCA CATTTGCCGT
      781 TTTGTAAACG AATTGCAGGA ATTGATAAAT AGTTAACTGC AGGGATGTCC ACGAGGTGTA
      841 TGAGACGTCA GAATTGGTTA ATTCGTCTCT CAGAATTGGG AGTCTACGAA GACCGGGGAC
      901 TTATCATCCA ACCTGTTAGA AAGGCGATGA AAGAGATAAA ACGAAAGGCT CAGTCGAAAG
      961 ACTGGGCCTT TCGTTTTACA ATTGGGCTAC CTTTTTTTTG TTTTGTTTGC AATGGTTAAT
     1021 CTATTGTTAA AATTTAAAGT TTCACTTGAA CTTTCAAATA ATGTTCTTAT ATTTGCAGTG
     1081 TCGAAAGAAA CAAAGTAGGA CTGATCGTCC ATCAATTTAA AATTTAAAAT AATGGAAAAA
     1141 AAGGAATTTC GTGTTTTGAT AAAATACTGT TTTCTGAAGG GAAAAAATAC AGTGGAAGCA
     1201 AAAACTTGGC TTGATAATGA GTTTCCGGAC TCTGCCCCAG GGAAATCAAC AATAATTGAT
     1261 TGGTATGCAA AATTCAAGCG TGGTGAAATG AGCACGGAGG ACGGTGAACG CAGTGGACGC
     1321 CCGAAAGAGG TGGTTACCGA CGAAAACATC AAAAAAATCC ACAAAATGAT TTTGAATGAC
     1381 CGTAAAATGA AGTTGATCGA GATAGCAGAG GCCTTAAAGA TATCAAAGGA ACGTGTTGGT
     1441 CATATCATTC ATCAATATTT GGATATGCGG AAGCTCTGTG CGAAATGGGT GCCGCGCGAG
     1501 CTCACATTTG ACCAAAAACA ACGACGTGTT GATGATTCTA AGCGGTGTTT GCAGCTGTTA
     1561 ACTCGTAATA CACCCGAGTT TTTCCGTCGA TATGTGACAA TGGATGAAAC ATGGCTCCAT
     1621 CACTACACTC CTGAGTCCAA TCGACAGTCG GCTGAGTGGA CAGCGACCGG TGAACCATCT
     1681 CCGAAGCGTG GAAAGACTCA AAAGTCCGCT GGCAAAGTAA TGGCCTCTGT TTTTTGGGAT
     1741 GCaCATGGAA TAATTTTTAT CGATTATCTT GAGAAGGGAA AAACCATCAA CAGTGACTAT
     1801 TATATGGCGT TATTGGAGCG TTTGAAGGTC GAAATCGCGG CAAAACGGCC CCACATGAAG
     1861 AAGAAAAAAG TGTTGTTCCA CCAAGACAAC GCACCGTGCC ACAAGTCATT GAGAACGATG
     1921 GCAAAAATTC ATGAATTGGG CTTCGAATTG CTTCCCCACC CGCCGTATTC TCCAGATCTG
     1981 GCCCCCAGCG ACTTTTTCTT GTTCTCAGAC CTCAAAAGGA TGCTCGCAGG GAAAAAATTT
     2041 GGCTGCAATG AAGAGGTGAT CGCCGAAACT GAGGCCTATT TTGAGGCAAA ACCGAAGGAG
     2101 TACTACCAAA ATGGTATCAA AAAATTGGAA GGTCGTTATA ATCGTTGTAT CGCTCTTGAA
     2161 GGGAACTATG TTGAATAAGC GGCCGCCACC GCGGTGGAGG GGAATTCCCA TGTCAGCCGT
     2221 TAAGTGTTCC TGTGTCACTC AAAATTGCTT TGAGAGGCTC TAAGGGCTTC TCAGTGCGTT
     2281 ACATCCCTGG CTTGTTGTCC ACAACCGTTA AACCTTAAAA GCTTTAAAAG CCTTATATAT
     2341 TCTTTTTTTT CTTATAAAAC TTAAAACCTT AGAGGCTATT TAAGTTGCTG ATTTATATTA
     2401 ATTTTATTGT TCAAACATGA GAGCTTAGTA CGTGAAACAT GAGAGCTTAG TACGTTAGCC
     2461 ATGAGAGCTT AGTACGTTAG CCATGAGGGT TTAGTTCGTT AAACATGAGA GCTTAGTACG
     2521 TTAAACATGA GAGCTTAGTA CGTGAAACAT GAGAGCTTAG TACGTACTAT CAACAGGTTG
     2581 AACTGCTGAT CTTCAGATCC TCTACGCCGG ACGCATCGTG GCCGGATCAA TTCCGTGATA
     2641 GGTGGGCTGC CCTTCCTGGT TGGCTTGGTT TCATCAGCCA TCCGCTTGCC CTCATCTGTT
     2701 ACGCCGGCGG TAGCCGGCCA GCCTCGCAGA GCAGGATTCC CGTTGAGCAC CGCCAGGTGC
     2761 GAATAAGGGA CAGTGAAGAA GGAACACCCG CTCGCGGGTG GGCCTACTTC ACCTATCCTG
     2821 CCCGGCTGAC GCCGTTGGAT ACACCAAGGA AAGTCTACAC GAACCCTTTG GCAAAATCCT
     2881 GTATATCGTG CGAAAAAGGA TGGATATACC GAAAAAATCG CTATAATGAC CCCGAAGCAG
     2941 GGTTATGCAG CGGAAAACGG AATTGATCCC CCTCGAATTG ACGCGTTTAC CAATGCTTAA
     3001 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
     3061 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
     3121 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
     3181 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
     3241 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
     3301 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
     3361 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
     3421 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
     3481 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
     3541 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
     3601 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
     3661 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
     3721 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
     3781 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
     3841 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
     3901 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
     3961 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA
     4021 ATAGGCGTAT CACGAGGCCC TTTCGTCACG CGTCCTCGGT A
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