LOCUS       pAAV_Syn_intron_        7166 bp    DNA        circular     14-JAN-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     UUID: 71935691-357e-4cb7-b903-d92b9b980e72
COMMENT     Type: Synthetic
COMMENT     ConfirmedExperimentally: 0
COMMENT     CreatedBy: SnapGene License
COMMENT     SequenceClass: UNA
COMMENT     TransformedInto: unspecified
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     repeat_region   4429..4569
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /locus_tag="AAV2 ITR"
                     /label="AAV2 ITR"
                     /ApEinfo_label="AAV2 ITR"
                     /ApEinfo_fwdcolor="#ffe4c4"
                     /ApEinfo_revcolor="#ffe4c4"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      6517..7105
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             5486..6346
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="?-lactamase"
                     /translation=MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ccffcc"
                     /ApEinfo_revcolor="#ccffcc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        5381..5485
                     /gene="<i>bla</i>"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#bbbbbb"
                     /ApEinfo_revcolor="#bbbbbb"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0
                     graphic_fg_color_0 #ffffff graphic_fg_color_1 #ffffff
                     outline_color_0 #000000 outline_color_1 #000000"
     misc_feature    1..130
                     /locus_tag="Left ITR"
                     /label="Left ITR"
                     /ApEinfo_label="Left ITR"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     promoter        146..593
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (<a
                     href="http://www.ncbi.nlm.nih.gov/pubmed/12595892"
                     title="http://www.ncbi.nlm.nih.gov/pubmed/12595892">Kügler
                     et al., 2003</a>)"
                     /locus_tag="hSyn promoter"
                     /label="hSyn promoter"
                     /ApEinfo_label="hSyn promoter"
                     /ApEinfo_fwdcolor="#bbbbbb"
                     /ApEinfo_revcolor="#bbbbbb"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0
                     graphic_fg_color_0 #ffffff graphic_fg_color_1 #ffffff
                     outline_color_0 #000000 outline_color_1 #000000"
     intron          596..728
                     /note="chimera between introns from human ?-globin and
                     immunoglobulin heavy chain genes"
                     /locus_tag="chimeric intron"
                     /label="chimeric intron"
                     /ApEinfo_label="chimeric intron"
                     /ApEinfo_fwdcolor="#444444"
                     /ApEinfo_revcolor="#444444"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0
                     graphic_fg_color_0 #000000 graphic_fg_color_1 #000000
                     outline_color_0 #ffffff outline_color_1 #ffffff"
     misc_feature    3942..4188
                     /note="WPRE3"
                     /locus_tag="WPRE3"
                     /label="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     rep_origin      4644..5024
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="M13 ori"
                     /label="M13 ori"
                     /ApEinfo_label="M13 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    4197..4420
                     /locus_tag="BGH polyA"
                     /label="BGH polyA"
                     /ApEinfo_label="BGH polyA"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2513..3076
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)<br/>A6"
                     /locus_tag="IRES"
                     /label="IRES"
                     /ApEinfo_label="IRES"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             complement(3083..3793)
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent
                     protein (<a
                     href="http://www.ncbi.nlm.nih.gov/pubmed/15558047"
                     title="http://www.ncbi.nlm.nih.gov/pubmed/15558047">Shaner
                     et al., 2004</a>)"
                     /translation=MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGA
                     LKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQY
                     ERAEGRHSTGGMDELYK*
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    3794..3799
                     /note="kozak<br/>"
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             complement(2513..2515)
                     /translation=M
                     /locus_tag="Start codon"
                     /label="Start codon"
                     /ApEinfo_label="Start codon"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     protein_bind    735..768
                     /bound_moiety="FLP recombinase from the <i>Saccharomyces
                     cerevisiae</i> 2? plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (<a href="http://www.ncbi.nlm.nih.gov/pubmed/21891781"
                     title="http://www.ncbi.nlm.nih.gov/pubmed/21891781">Turan
                     and Bode, 2011</a>)"
                     /locus_tag="FRT (minimal)"
                     /label="FRT (minimal)"
                     /ApEinfo_label="FRT (minimal)"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    819..852
                     /locus_tag="frt F5"
                     /label="frt F5"
                     /ApEinfo_label="frt F5"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     protein_bind    complement(3812..3845)
                     /bound_moiety="FLP recombinase from the <i>Saccharomyces
                     cerevisiae</i> 2? plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (<a href="http://www.ncbi.nlm.nih.gov/pubmed/21891781"
                     title="http://www.ncbi.nlm.nih.gov/pubmed/21891781">Turan
                     and Bode, 2011</a>)"
                     /locus_tag="FRT (minimal)(1)"
                     /label="FRT (minimal)(1)"
                     /ApEinfo_label="FRT (minimal)"
                     /ApEinfo_fwdcolor="#31849b"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    complement(3896..3929)
                     /locus_tag="frt F5(1)"
                     /label="frt F5(1)"
                     /ApEinfo_label="frt F5"
                     /ApEinfo_fwdcolor="#a6acb3"
                     /ApEinfo_revcolor="#a6acb3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             complement(1892..1912)
                     /note=""
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /protein_id=
                     /translation=PKKKRKV
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1916..2506
                     /locus_tag="WGA_Codon opt"
                     /label="WGA_Codon opt"
                     /ApEinfo_label="WGA_Codon opt"
                     /ApEinfo_fwdcolor="#ff9900"
                     /ApEinfo_revcolor="#ff9900"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    863..1915
                     /locus_tag="Cre_codon opt"
                     /label="Cre_codon opt"
                     /ApEinfo_label="Cre_codon opt"
                     /ApEinfo_fwdcolor="#339966"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_graphicformat="arrow_data {{0 .5 .1 .5 .1 -.5 0
                     -.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     711..729
                     /PCR_conditions="primer sequence:TTGCCTTTCTCTCCACAGGT"
                     /note="Synthetic intron, forward primer"
                     /locus_tag="pMT2-F"
                     /label="pMT2-F"
                     /ApEinfo_label="pMT2-F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     primer_bind     complement(3996..4016)
                     /PCR_conditions="primer sequence:CATAGCGTAAAAGGAGCAACA"
                     /note="WPRE, reverse primer"
                     /locus_tag="WPRE-R"
                     /label="WPRE-R"
                     /ApEinfo_label="WPRE-R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
      121 AGGGGTTCCT GCGGCCGCCA ATTGaagtgc aagtgggttt taggaccagg atgaggcggg
      181 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc
      241 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt
      301 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc
      361 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc
      421 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga
      481 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc
      541 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgtaag
      601 tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag
      661 aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct
      721 ctccacaggc tagcgaagtt cctattctct agaaagtata ggaacttcgc agaatggtag
      781 ctggattgta gctgctatta gcaatatgaa acctcttaga agttcctatt cttcaaaagg
      841 tataggaact tcGATATCga atTCAATCTC CGTCTTCAAG TAGCCTCACC ATGGCGCCAG
      901 TCTCAGAATC TAGATTTCTG ATGTAATTCA TTACGATGTT CACGTTGGTC CAACCACCTG
      961 CCTGCATGAT CTCAGGGATG GAGACACCTG CACGGGCCAT ATCTCTAGCC GCTCCTACTC
     1021 GTGCACTATG ACCACTCCAT GCCAAATAGC GCTGGCCACT GTCATCTTTG GCACCATAAA
     1081 TTAACCTATG AGTCGCTTCA AATATCCCTT CGAGGGCCCT AGTGCTGAGT TGGCTTGTAG
     1141 CTGATGGTGC AGCCACCCCG TTTTTCCTAA CTCTGCAGAA AAGATAGTTG TTGGGATCAT
     1201 CTGCAACTCC GCTAACTGAG ATCCAGCGTT CTACGAGCTT TGTAACCCCG AGGCTCAGTG
     1261 CTTTTTCCAC ACCAGCAGTA GAGACCAGGG TTTTGGTCCG CCCAATGTGG ATAAGCATCC
     1321 GGCCACCATC TGTGCGAGAA ATGTCTTTAA CTCGTATCCG AGCAATTTCA GCAATCCTAA
     1381 GCAAGGTGTT GTAGGCGATC CCTAAAAAAG CCAAATTGCG AATGTCCTGA CAGCGGTCGG
     1441 AGTTCTCCAT GAGACTTCGG ACCTGGTCAA AATCGGTCCT CTCAAACGCT AGAGCCTGCT
     1501 TGGCTCTTTC CCCAGCGTCC ACATTCTCCT TCCTTATCCG CCTCATGACT AAAGACACGG
     1561 CATTGCTATC ACTTGGACGG GGCAGGCCGG AGCGTCGATG GAGCATGTTC AGCTGTCCCA
     1621 GGTGCTGCTG GATGGTTTTC ACAGCCAGCC CCCTCGCCTG CAGGTACAGC AAATAGTCTC
     1681 TTACATCTTC TGGCTCTGCA GGAAACCATT TACGGTTATT CAGCTTGCAC CAAGCAGCCC
     1741 AGGAGCGGCA GACCGACAGC AGCATCTTCC AGGTATGCTC TGAGAATGCT TGTCTGTCCC
     1801 GGAACATGTC CATTAGGTTC TTACGCACCT CATCAGATGT GGCATCCACT GGCAGCGCAG
     1861 GCAAGTTCTG GTGCACAGTG AGGAGGTTGC TCACCTTCCG TTTCTTCTTG GGCATGTCAC
     1921 AGCCTCCAGA TTGACAGCCT GCCCCGCAAT AGCCAGGACC AATCCCACAT GAGCCCCACT
     1981 TGGAGCAACA GTAGTTATTC GTACACACCC GGCCGCCAGC ATCCTTCCCA CAGGGCTTGT
     2041 CTGTACTACA GGCTCCCGAC TGGCAGCCGC CTCCACAGAA CTCGCTGCCC AGTCCGCAGA
     2101 ACCCCCACTG GGAACAGCAG AGATTATTCG GGCACAGTTT CCCTCCGCTT TGTGAGCCGC
     2161 ACTTGATGTC TGCCCTGCAG GGACCCCCTT GGCAGCCGGC GCCACAGTAC TCAGCTCCGA
     2221 AGCCACAGTG TCCATACTGG GAACAACAGT GGTTGTTTGG GCAGGTGGCG CCTCCAGCTT
     2281 GCGACCCACA CCGCTTACTT GTCCAGCAGG CTCCATTCTG GCATCCCTTG CCGCAGTAGT
     2341 CTCCCCCCAT GCCACAGTAG CCGTACTGGC TGCAGCATAA ATTGTTAGGA CATTCCATAT
     2401 TGGAACCCTG CTCTCCACAT CTCTGGGCCT GGGCAGTGGC TGCGGCAAAG GCTAGGAAGA
     2461 CTGCAGCCCC CAGCGTCAAT GCCATTGTAG ACATCATTTT TCTCATacta gtCATATTAT
     2521 CATCgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgtttttta
     2581 acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat
     2641 ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga
     2701 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
     2761 tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct
     2821 gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt
     2881 ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa
     2941 tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata
     3001 tggtggaaaa taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag
     3061 taacgttagg ggggggacgc gtttacttgt acagctcgtc catgccgccg gtggagtggc
     3121 ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
     3181 ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg
     3241 tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct
     3301 cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg
     3361 tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt
     3421 agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
     3481 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg
     3541 ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga
     3601 tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc
     3661 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
     3721 cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc
     3781 ccttgctcac CATGGTGGCg gatcctcgcg agaagttcct atactttcta gagaatagga
     3841 acttcttgcc ttaacccaga aattatcact gttattcttt agaatggtgc aaagagaagt
     3901 tcctatacct tttgaagaat aggaacttca ccggtGTATA Ctaatcaacc tctggattac
     3961 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4021 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4081 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     4141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtggt cgaccttgtg
     4201 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     4261 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     4321 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     4381 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg GCGGCCGCAG GAACCCCTAG
     4441 TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA
     4501 AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT
     4561 GCCTGCAGGG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC
     4621 GCATACGTCA AAGCAACCAT AGTacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4681 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     4741 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4801 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4861 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4921 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4981 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggCCTATT GGTTAAAAAA
     5041 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT
     5101 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC
     5161 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA
     5221 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG
     5281 CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT
     5341 GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT
     5401 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
     5461 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC
     5521 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA
     5581 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG
     5641 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT
     5701 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG
     5761 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC
     5821 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC
     5881 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA
     5941 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC
     6001 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT
     6061 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA
     6121 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA
     6181 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA
     6241 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA
     6301 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT
     6361 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT
     6421 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG
     6481 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT
     6541 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
     6601 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
     6661 TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
     6721 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT
     6781 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG
     6841 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
     6901 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
     6961 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
     7021 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC
     7081 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
     7141 CTTTTGCTGG CCTTTTGCTC ACATGT   
//
