LOCUS       pCnCre                  4229 bp    DNA     circular SYN 12-DEC-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..674)
                     /label=ori_pLO3
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    681..1462
                     /label=CymR
                     /note="color: #ffef86"
     misc_feature    1463..1505
                     /label=Ptac
                     /note="color: #ffef86"
     misc_feature    1506..1538
                     /label=CymO
                     /note="color: #a6acb3"
     misc_feature    1608..2639
                     /label=Cre
                     /note="color: #9eafd2"
     terminator      2659..3059
                     /label=rrnB
                     /note="/dnas_title=rrnB"
                     /note="/vntifkey=43"
                     /note="color: #d59687"
     misc_feature    3349..4008
                     /label=CmR
                     /note="/vntifkey=21"
                     /note="color: #c7b0e3"
ORIGIN
        1 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
       61 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      121 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      181 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      241 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      301 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      361 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
      421 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
      481 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
      541 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
      601 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      661 atcttttcta cggggtcgac acggatggcc tttttgcgtt tctacaaact cttttgttta
      721 tttttctaaa tacattcaaa tatgtatccg ctcatgaaca ataaaactgt ctgcttacat
      781 aaaacattga aaaaggaaga gtatgagtcc aaagagaaga acacaggcag agcgcgcaat
      841 ggagacccag ggcaagttga ttgcagcggc cctgggggtt ttacgggaaa aaggttacgc
      901 gggattccgg atcgcagatg tgcccggtgc tgcaggtgtc tcgagaggag cgcagagcca
      961 tcatttcccg acaaagcttg agcttctgct tgccactttt gaatggcttt acgaacagat
     1021 caccgaacgc agtcgggctc gattagcgaa attgaagcca gaggatgacg tcatccagca
     1081 aatgctggac gacgccgccg aatttttcct cgacgatgac ttctctatca gccttgattt
     1141 gattgtggct gccgaccggg atccagcgtt acgcgagggt attcagcgca cggtagagag
     1201 gaatcggttt gtcgtcgagg atatgtggct tggtgttctg gtgagccgtg gtctttcgcg
     1261 tgatgatgca gaagatatcc tttggttgat attcaattcg gtgcgtgggc ttgctgttcg
     1321 tagcctatgg cagaaggaca aagaacgctt tgagcgtgtc aggaactcga cactcgaaat
     1381 tgcgcgagag cggtacgcga aattcaagcg ctagcaaaaa acccctcaag acccgtttag
     1441 aggccccaag gggttatgct agaatgagct gttgacaatt aatcatcggc tcgtataatg
     1501 tgtggaacaa acagacaatc tggtctgttt gtattataga attcgagctc ggtacccggg
     1561 gatcctctag aaataatttt gtttaacttt aagaaggaga tatacatatg tccaatttac
     1621 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
     1681 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
     1741 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
     1801 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
     1861 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
     1921 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
     1981 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
     2041 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
     2101 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
     2161 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
     2221 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
     2281 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
     2341 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
     2401 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
     2461 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     2521 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata
     2581 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattaga
     2641 agcttattta aatggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa
     2701 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt
     2761 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     2821 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     2881 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     2941 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     3001 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     3061 ttgcgtttct acaaactctt tttgtttatt tttctaaata cattcaaata tgtatccgct
     3121 catgaagcgg ccgctcatga cgaataaata cctgtgacgg aagatcactt cgcagaataa
     3181 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga
     3241 gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     3301 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     3361 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     3421 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     3481 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     3541 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     3601 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     3661 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     3721 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     3781 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     3841 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     3901 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     3961 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     4021 agttattggt gcccttaaac gcctggtgct acgcctgaat aagtgataat aagcggatga
     4081 atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat
     4141 agccgcttat gtctattgct ggtttaccgg tttattgact accggaagca gtgtgaccgt
     4201 gtgcttctca aatgcctgag gccagttaa
//
