LOCUS       Exported                4980 bp ds-DNA     circular SYN 25-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1661..1688
                     /label=pCAG_F
     CDS             1768..1791
                     /label=Strep-Tag II
                     /note="codon_start: 1 product: peptide that binds 
                     Strep-Tactin(R), an engineered form of streptavidin 
                     transl_table: 1 translation: WSHPQFEK"
     CDS             1828..1851
                     /label=Strep-Tag II
                     /note="codon_start: 1 product: peptide that binds 
                     Strep-Tactin(R), an engineered form of streptavidin 
                     transl_table: 1 translation: WSHPQFEK"
     CDS             1858..1881
                     /label=FLAG
                     /note="codon_start: 1 product: FLAG(R) epitope tag, 
                     followed by an enterokinase cleavage site transl_table: 1 
                     translation: DYKDDDDK"
     primer_bind     complement(1965..1985)
                     /label=pCAG_R
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgatc
     1741 gacggtatcg atgccaccat ggctagctgg agccacccgc agttcgagaa aggtggaggt
     1801 gcccgaggtg gatcgggagg tggatcgtgg agccacccgc agttcgaaaa aggattcgat
     1861 tacaaggatg acgatgacaa gggtaccccc gggtgatatc ttaattaatt aactcgagga
     1921 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     1981 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     2041 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     2101 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     2161 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     2221 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     2281 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     2341 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     2401 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     2461 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2521 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2581 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2641 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2701 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2761 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     2821 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     2881 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2941 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     3001 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3061 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3121 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3421 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3661 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3901 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3961 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4021 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4081 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4141 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4201 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4261 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4321 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4381 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4441 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4501 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4561 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4621 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4681 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4741 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4801 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4861 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4921 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
