LOCUS       063_pCAGGS-OsTIR       10524 bp    DNA     circular SYN 15-JAN-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   NYU School of Medicine
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
COMMENT     Sequence Label: KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-Ne...
FEATURES             Location/Qualifiers
     source          1..10524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1740..1778
                     /label=F primer
                     /label=240
     misc_feature    1760..3487
                     /label=OsTIR F74G
     CDS             1760..3487
                     /codon_start=1
                     /label=Feature 27
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKILR
                     "
     polyA_signal    3643..3698
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4059..4075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4083..4099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4107..4113,4114..4131,4132..4137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4152..4173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    4237..4270
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        4334..4833
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        4845..4892
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4911..5714
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5752..5976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6031..6064
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    6105..6867
                     /label=R-HA
     promoter        complement(6890..6908)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6929..6945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6953..6969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6977..6983,6984..7001,7002..7007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7022..7043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7331..7919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8090..8881,8882..8950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8951..9055)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9081..9536)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9678..9694
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9701..9719
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    9726..10515
                     /label=L-HA
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcct cgagaattct
     1741 cacgcgtctg caggatatca tgacatactt tcctgaagag gtcgtcgaac acatttttag
     1801 cttcctgcct gcacagagag atagaaacac agtgagcctg gtctgcaaag tgtggtacga
     1861 gatcgaacgc ctgagccgga gaggagtgtt cgtcggcaac tgctatgctg tgagagcagg
     1921 cagggtcgcc gctaggtttc caaatgtgcg cgcactgacc gtcaagggga aaccccacgg
     1981 cgccgacttt aacctggtgc cccctgattg gggaggatac gccggccctt ggatcgaggc
     2041 agccgctcgc ggctgtcatg gactggagga actgcgcatg aagcgaatgg tggtctctga
     2101 cgaaagtctg gagctgctgg ctcggagctt ccctaggttt cgcgcactgg tgctgatttc
     2161 ttgcgaaggc ttcagcaccg atggactggc agccgtggcc tcccactgta agctgctgcg
     2221 ggagctggac ctccaggaga atgaagtgga ggatagaggc cccagatggc tgtcttgctt
     2281 cccagactca tgtaccagcc tggtgtccct gaactttgcc tgcatcaaag gcgaagtgaa
     2341 tgctgggtcc ctggagcggc tggtctcaag aagccccaac ctgaggtctc tgcggctgaa
     2401 ccggagcgtg agcgtggaca ctctggctaa gattctgctg agaaccccta acctggagga
     2461 tctgggaacc ggcaatctga cagacgattt ccagacagaa tcctacttta aactgacttc
     2521 tgccctggag aagtgtaaaa tgctgaggag tctgtcagga ttctgggatg cttcacccgt
     2581 gtgcctgagc tttatctacc ctctgtgtgc acagctgaca ggcctgaacc tgagctatgc
     2641 accaaccctg gacgccagtg atctgacaaa gatgatctca cgctgcgtga aactccagcg
     2701 actgtgggtg ctggactgta tttccgataa ggggctccag gtggtcgcca gctcctgcaa
     2761 ggacctccag gagctgagag tgttcccatc tgatttttac gtggccggat atagtgctgt
     2821 cactgaggaa ggcctggtgg cagtctcact gggatgccca aagctgaaca gcctgctgta
     2881 tttctgtcat cagatgacta atgctgcact ggtgaccgtc gccaagaact gccctaattt
     2941 cacccgattt cggctgtgta ttctggaacc aggcaaaccc gacgtggtca catcccagcc
     3001 actggatgaa gggtttggag ctatcgtgag agagtgcaag ggactccaga ggctgagcat
     3061 ttccggcctg ctgacagaca aagtgttcat gtacatcggc aagtatgcta agcagctgga
     3121 gatgctgagc attgcatttg ccggagactc cgataagggc atgatgcacg tgatgaacgg
     3181 gtgtaagaat ctgcgaaaac tggaaatccg ggacagccct ttcggggatg ccgctctgct
     3241 gggaaacttt gccagatacg agacaatgag gagcctgtgg atgtctagtt gcaatgtgac
     3301 tctgaagggc tgtcaggtcc tggctagtaa aatgcctatg ctgaacgtgg aagtcattaa
     3361 tgagcgggac gggtctaacg aaatggagga aaatcatggc gacctgccaa aggtggagaa
     3421 actgtatgtg tatcggacca ccgcaggggc aagagatgat gctcccaact ttgtgaagat
     3481 tctgagatga cccgggtaag cggcctcagg tgcaggctgc ctatcagaag gtggtggctg
     3541 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     3601 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     3661 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     3721 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     3781 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     3841 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     3901 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     3961 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     4021 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     4081 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     4141 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     4201 cagtcgggaa acctgtcgtg ccagcggatc aattccgaag ttcctattct ctagaaagta
     4261 taggaacttc aggtctgaag aggagtttac gtccagccaa gctagcttgg ctgcaggtcg
     4321 tcgaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     4381 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     4441 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     4501 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     4561 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     4621 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     4681 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     4741 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     4801 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcctgttgac aattaatcat
     4861 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atgggatcgg
     4921 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4981 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5041 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5101 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5161 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5221 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5281 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5341 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5401 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5461 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5521 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5581 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5641 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5701 acgagttctt ctgaggggat caattctcta gagctcgctg atcagcctcg actgtgcctt
     5761 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5821 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5881 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5941 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6001 ggggctcgac tagagcttgc ggaacccttc gaagttccta ttctctagaa agtataggaa
     6061 cttcatcagt caggtacata atggatccgg aagaggggga agggattctc ccaggcccag
     6121 ggcggtcctc agaagccagg aggcagcaga gaactcccag aaaggtattg caacactccc
     6181 ctcccccctc cggagaaggg tgcggccttc tccccgccta ctccactgca gctcccttac
     6241 tgataacaac tcagagcgac tttgggagag caagtgcttc ctgcctccaa aacagcccaa
     6301 ctgagccctc gctccttccc tccactcccc ggagtgcgcg atggaggtct ggctcagcac
     6361 gcccctcttg aggcaactca agtcggaaac gtgcttgcac ccgccccgca gccgctcagc
     6421 cctactgccc gtccccgccc ccagcgcgcg cttcctgcca cgttgcgcag gggcgcgcgg
     6481 ccagactctg cggcgcgggg ccgaggggag ggccggaacc tgggagcgcc tcctcgccgc
     6541 ccccgctggc cggcggatgg actcaacttg cacgaacacg agccaatggc aagggccagt
     6601 tttctgggcc ccgagagcca atcagacgac gaggcccggc cggcggcggg taaaacgact
     6661 cccccagagg aaggggaggg tgggcggccg ctcgcgcgcg agctactttc gctgaccctc
     6721 cctcccctcc cccgccccgc cagaggccga ccgcgcccgc acgtccagct cgcctcaccc
     6781 cacctacctc ccgccccacc cagtgggcag agcgaggctg ccggcggctg cgcactccgg
     6841 ctgccgttaa ctggcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     6901 ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7021 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7081 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7741 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9061 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     9121 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     9181 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     9241 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     9301 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     9361 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     9421 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     9481 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     9541 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9601 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9661 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
     9721 cgaattgggt accataaatg gagaaaaagg agagaggcat tcatgggagt ggaaagttaa
     9781 gccttaggtt ggattctcaa tacatctatt gttgtaaaac tccctggact gagaataggc
     9841 ccaaatgtgg aacaccacct gacgggagag gtgatagaca ctgaaaatta aggatcaagg
     9901 caaaggatca acaaaaagtg tactaaggag ttataaaaga actgcagtgt cgaggcccca
     9961 gctacagcct cgatttgtgg tgtatgtaac taatctgtct ggtttcatga gtcatcagac
    10021 ttctaagatc aggaaaggga aaatgccaat gctctgtcta ggggttggat aagccagtct
    10081 aataaatgaa aatggggcta aaatgagtgt tctaaaatac cttttgataa ggctgcagaa
    10141 ggagcgggag aaatggatat gaagtactgg gctctttaaa aatgattaaa attctgctta
    10201 catagtctaa ctcgcgacac tgtaatttca tactgtagta aggatctcaa gcaggagagt
    10261 ataaaactcg ggtgagcatg tctttaatct acctcgatgg aaaatactcc gaggcggatc
    10321 acaagcaata ataacctgta gttttgctgc ataaaacccc agatgactac ctatcctccc
    10381 attttcctta tttgccccta ttaaaaaact tcccgacaaa accgaaaatc tgtgggaagt
    10441 cttgtccctc caattttaca cctgttcaat tcccctgcag gacaacgccc acacaccagg
    10501 ttagccttta agcctctcga ggtc
//
