LOCUS       84._pHR-Flag-IF1        9658 bp DNA     circular SYN 27-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Lim Lab
  TITLE     Direct Submission
  JOURNAL   Exported Feb 27, 2025 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9658
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     LTR             1..341
                     /label=3' LTR (SIN)
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     LTR             108..341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     promoter        1172..1276
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1277..2137
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1277 .. 1345 / #ccffcc / signal sequence
                        2: 1346 .. 2137 / #ccffcc
                     Cleavage site after base 1345"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1475..2134
                     /label=AmpR
                     /note="/ApEinfo_fwdcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=yellow"
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2232..2914
                     /label=ColE1 origin
                     /note="/ApEinfo_fwdcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=gray50"
                     /note="color: #808080"
     rep_origin      2308..2896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        3130..3456
                     /label=SV40 - Invitrogen
                     /note="/ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#0000ff"
                     /note="color: #0000ff"
     promoter        3142..3471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    complement(3170..3196)
                     /label=sgSV40 P1
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff8000"
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(3242..3268)
                     /label=sgSV40 P1(1)
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=sgSV40 P1
                     /ApEinfo_revcolor=#ff8000"
                     /note="color: #ff8000; direction: LEFT"
     promoter        3275..3471
                     /label=SV40 early promoter(2)
                     /note="/ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=SV40 early promoter
                     /ApEinfo_revcolor=cornflower blue"
                     /note="color: #6495ed"
     promoter        3275..3456
                     /label=SV40 early promoter(1)
                     /note="/ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=SV40 early promoter
                     /ApEinfo_revcolor=cornflower blue"
                     /note="color: #6495ed"
     rep_origin      3322..3457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     misc_RNA        complement(3407..3426)
                     /label=sgEGFP NT1
                     /note="/ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff8040"
                     /note="color: #ff8040; direction: LEFT"
     promoter        3457..3471
                     /label=SV40 early promoter
                     /note="/ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=cornflower blue"
                     /note="color: #6495ed"
     CDS             3671..4128
                     /label=gpt
                     /label=gpt; xanthine guanine phosphoribosyl transferase
                     /note="/ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: RIGHT"
     intron          4605..4670
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     CDS             4800..4820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_site      5151..5169
                     /label=SV40 pA
                     /note="/ApEinfo_fwdcolor=#ccff66
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ccff66"
                     /note="color: #ccff66"
     polyA_signal    complement(5184..5375)
                     /label=SV40 late polyA
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_signal    5245..5379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     polyA_site      5245..5375
                     /label=SV40 early poly A
                     /note="/ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#80ff00"
                     /note="color: #80ff00"
     LTR             5547..6180
                     /label=5' LTR
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    6227..6352
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    6232..6369
                     /label=HIV-1 genomic RNA packaging signal
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_signal     6289..6292
                     /label=5' splice site
                     /note="/ApEinfo_fwdcolor=#80ff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #80ff80"
     CDS             6336..6338
                     /label=gag ORF start site
                     /note="/ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: RIGHT"
     modified_base   6378..6379
                     /label=gag ORF disrupted by a frame shift
                     /label=gag ORF disrupted by a frame shift; ClaI site fille
                     /note="/ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #ff0000"
     misc_feature    6849..7082
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     repeat_region   7570..7749
                     /label=cPPT
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /vntifkey=34"
                     /note="color: #ffc0cb"
     misc_feature    7596..7713
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     7725..7748
                     /label=cPPT_5'seq
                     /note="color: black; sequence: cagggacagcagagatccagtttg"
     promoter        7782..8273
                     /label=SFFV promoter (mutated) promoter
                     /label=SFFV promoter (mutated)
                     /note="color: #808080"
     promoter        7866..8273
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     7918..7937
                     /label=SFFV_Distal_GMA.FOR
                     /note="color: black; sequence: TCAGATCAAGGGCGGGTACA"
     primer_bind     8193..8212
                     /label=cw10
                     /note="color: #ff8040; direction: RIGHT"
     primer_bind     8286..8324
                     /label=Fragment.FOR (2)
                     /note="color: black; sequence: 
                     ctctcgagaattctcacgcgtcgccgccaccATGAAGAC"
     misc_feature    8307..8316
                     /label=Kozak
                     /note="color: #a6acb3"
     misc_feature    8317..8364
                     /label=Cleavable Signal Sequence
                     /note="color: #a6acb3"
     CDS             8365..8388
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     primer_bind     8374..8416
                     /label=Fragment.FOR (3)
                     /note="color: black; sequence: 
                     GATGACGATGATAAAGGATCCGCCCTCAAAAAGCACCACGAAA"
     primer_bind     8374..8394
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     GATGACGATGATAAAGGATCCATAGTGATTATAACGGTCGTTGCGG"
     misc_feature    8395..8517
                     /label=1HF9
                     /note="color: #a6acb3"
     CDS             8518..8793
                     /codon_start=1
                     /label=Fibcon
                     /note="color: #993366"
                     /translation="MLDAPTDLQVTNVTDTSITVSWTPPSATITGYRITYTPSNGPGEP
                     KELTVPPSSTSVTITGLTPGVEYVVSVYALKDNQESPPLVGTQTTGG"
     primer_bind     complement(8779..8814)
                     /label=Fragment.REV (2)
                     /note="color: black; sequence: 
                     CGTTATAATCACTATGGATCCgccgccggtggtctg"
     primer_bind     complement(8779..8801)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     aagaatccccagaatGGATCCgccgccggtggtctg"
     primer_bind     8794..8824
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     GATGACGATGATAAAGGATCCATAGTGATTATAACGGTCGTTGCGG"
     CDS             8800..8958
                     /codon_start=1
                     /label=ICAM
                     /note="color: #993366"
                     /translation="IVIITVVAAAVIMGTAGLSTYLYNRQRKIKKYRLQQAQKGTPMKP
                     NTQATPP"
     primer_bind     complement(8934..8978)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     gcggccgcaagtcaaggatcTTACGGTGGTGTAGCCTGAGTATTT"
     misc_feature    8959..8978
                     /label=BamH1 right
                     /note="color: #a6acb3"
     misc_feature    8959..8962
                     /label=BamHI left
                     /note="color: #a6acb3"
     misc_feature    8985..8991
                     /label=AarI
                     /note="color: #ff0080"
     misc_feature    8996..8999
                     /label=Part D
                     /note="color: #00ffff"
     misc_feature    9031..9038
                     /label=SbfI
                     /note="color: #ff0000"
     enhancer        9067..9658
                     /label=WPRE
                     /note="/ApEinfo_fwdcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=pink
                     /vntifkey=9"
                     /note="color: #ffc0cb"
     misc_feature    9068..9656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    complement(9090..9113)
                     /label=cw11
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     complement(9121..9141)
                     /label=RHLp66 - pHR Rev
                     /note="color: black; sequence: catagcgtaaaaggagcaaca"
     CDS             complement(9539..9550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 9538"
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     5461 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     5521 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     5581 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     5641 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     5701 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     5761 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     5821 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     5881 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     5941 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     6001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     6301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     6361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     6421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     6481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     6601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     6661 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     6721 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     6781 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     6841 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     6901 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     6961 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7021 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     7741 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
     7801 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     7861 ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca
     7921 gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag
     7981 cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc
     8041 gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca
     8101 tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac
     8161 caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag
     8221 ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggggggatc
     8281 tggagctctc gagaattctc acgcgtcgcc gccaccatga agacaattat cgccctcagt
     8341 tacatttttt gtcttgtttt tgcggattat aaagatgacg atgataaagg atccgccctc
     8401 aaaaagcacc acgaaaacga gatatcacat cacgctaaag agattgaacg gcttcaaaag
     8461 gaaattgagc ggcataagca gtccataaag aaactgaagc aaagtgagga cgatgacatg
     8521 ctggatgcgc cgaccgatct gcaggtgacc aacgtgaccg ataccagcat taccgtgagc
     8581 tggaccccgc cgagcgcgac cattaccggc tatcgcatta cctatacccc gagcaacggc
     8641 ccgggcgaac cgaaagaact gaccgtgccg ccgagcagca ccagcgtgac cattaccggc
     8701 ctgaccccgg gcgtggaata tgtggtgagc gtgtatgcgc tgaaagataa ccaggaaagc
     8761 ccgccgctgg tgggcaccca gaccaccggc ggcggatcca tagtgattat aacggtcgtt
     8821 gcggcggctg tcattatggg gacggctggc ctttctacct acctgtataa ccgccaacgc
     8881 aagataaaaa agtataggct ccaacaggcc caaaagggga ctccaatgaa gccaaatact
     8941 caggctacac caccgtaaga tccttgactt gcggccgcaa ctcccacctg caacatgcgt
     9001 gactgactga ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     9061 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     9121 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     9181 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     9241 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     9301 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     9361 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     9421 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     9481 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     9541 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     9601 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcat
//
