LOCUS       Exported                6573 bp ds-DNA     circular SYN 17-JAN-2025
DEFINITION  current pMXs-mIsll1     coverage:   10.1x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    573..930
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1415..1790
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     protein_bind    1833..1857
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2041..3087
                     /codon_start=1
                     /product="Isl1(human and mouse seq the same)"
                     /label=Isl1
                     /translation="MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKC
                     AECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIEC
                     FRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPI
                     SARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSP
                     RVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANP
                     VEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPN
                     SMVASPIEA"
     protein_bind    complement(3151..3175)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3192..3780
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3663..3674)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3960..4429
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(5496..6356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGGRGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6357..6461)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(6491..6573,1..511)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tagaaaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
       61 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
      121 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
      181 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
      241 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
      301 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
      361 ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
      421 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
      481 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc ggagacccct
      541 gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg
      601 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
      661 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
      721 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtccaaaaa
      781 tcccgatcgt tttggactct ttggtgcacc cccctaatag gagggatatg tggttctggt
      841 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
      901 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgcccccat atggccatat gagatcttat atgggggcac
     1441 ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc
     1501 tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg
     1561 cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca
     1621 cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca
     1681 cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg
     1741 ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatttag
     1801 ctagttaatc gacggtatcg ataagctatc aaacaagttt gtacaaaaaa gcaggcttgg
     1861 cttaaatcgg actcccagat ctgcgagggc gcggcgcagc cgggcagctg tttcccccag
     1921 ttttggcaac cccgggggcc actatttgcc acctagccac agcaccagca tcctctctgt
     1981 gggctattca ccaattgtcc aaccaccatt tcactgtgga cgttactccc tcttacagat
     2041 atgggagaca tgggcgatcc accaaaaaaa aaacgtctga tttccctgtg tgttggttgc
     2101 ggcaatcaaa ttcacgacca gtatattctg agggtttctc cggatttgga gtggcatgca
     2161 gcatgtttga aatgtgcgga gtgtaatcag tatttggacg aaagctgtac gtgctttgtt
     2221 agggatggga aaacctactg taaaagagat tatatcaggt tgtacgggat caaatgcgcc
     2281 aagtgcagca taggcttcag caagaacgac ttcgtgatgc gcgcccgctc taaggtgtac
     2341 cacatcgagt gtttccgctg tgtagcctgc agccgacagc tcatcccggg agacgaattc
     2401 gccctgcggg aggatgggct tttctgccgt gcagaccacg atgtggtgga gagagccagc
     2461 ctgggagctg gagaccctct cagtcccttg catccagcgc ggcctctgca aatggcagcc
     2521 gaacccatct cggctaggca gccagctctg cggccgcacg tccacaagca gccggagaag
     2581 accacccgag tgcggactgt gctcaacgag aagcagctgc acaccttgcg gacctgctat
     2641 gccgccaacc ctcggccaga tgcgctcatg aaggagcaac tagtggagat gacgggcctc
     2701 agtcccagag tcatccgagt gtggtttcaa aacaagcggt gcaaggacaa gaaacgcagc
     2761 atcatgatga agcagctcca gcagcagcaa cccaacgaca aaactaatat ccaggggatg
     2821 acaggaactc ccatggtggc tgctagtccg gagagacatg atggtggttt acaggctaac
     2881 ccagtagagg tgcaaagtta ccagccgccc tggaaagtac tgagtgactt cgccttgcaa
     2941 agcgacatag atcagcctgc ttttcagcaa ctggtcaatt tttcagaagg aggaccaggc
     3001 tctaattcta ctggcagtga agtagcatcg atgtcctcgc agctcccaga tacacccaac
     3061 agcatggtag ccagtcctat tgaggcatga ggaacattca ttcagatgtt ttgttttgtt
     3121 ttgttttgtt tttttcccct gttggagaat acccagcttt cttgtacaaa gtggttcgat
     3181 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3241 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3301 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3361 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3421 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3481 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3541 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3601 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3661 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3721 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3781 atcgataccg tcgacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     3841 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     3901 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     3961 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     4021 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4081 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     4141 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     4201 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     4261 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     4321 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     4381 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     4441 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
     4501 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4561 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4621 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4681 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4741 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4801 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4861 cgaccctgcc gcttaccgga tacctgtccg cccttctccc ttcgggaagc gtggcgcttt
     4921 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4981 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5041 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5101 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5161 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5221 aagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5281 gcaagcagca gattacgcgc agaaaaaaag gatcctcaag aagatccttt gatcttttct
     5341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattgtggt catgagatta
     5401 tcaaaaagga tcttcacttg agatcctttt ctctcccccc tgcggccggc cgtcgaatca
     5461 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5521 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5581 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5641 ccacgcccac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5701 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5761 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5821 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5881 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5941 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6001 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6061 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6121 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6181 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6241 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6301 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6361 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6421 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6481 ccacctgcat aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     6541 tttgcaaggc atggaaaaat acataactga gaa
//
