LOCUS       Exported                8495 bp ds-DNA     circular SYN 17-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     109..128
                     /label=oSEQ054 pMXs-fwd-seq
     primer_bind     complement(198..211)
                     /label=oSEQ013 flipped_WPRE_rev
     protein_bind    222..246
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(223..241)
                     /label=?oSEQ049 attR1-rev
     primer_bind     265..284
                     /label=Lhx3_fwd
     primer_bind     265..284
                     /label=Lhx3_part1_fwd
     primer_bind     270..284
                     /label=oTA100 GG_entry_upstream_rev
     primer_bind     complement(270..284)
                     /label=oTA101 GG_entry_downstream_fwd
     primer_bind     285..306
                     /label=Ngn2_1_fwd
     misc_feature    289..297
                     /label=Kozak sequence
                     /note="Partial (missing last G) vertebrate consensus 
                     sequence for strong initiation of translation (Kozak, 
                     1987)"
     CDS             295..1083
                     /codon_start=1
                     /product="Mouse Ngn2 "
                     /label=mNgn2
                     /translation="MFVKSETLELKEEEEVLMLLGSASPASATLTPMSSSADEEEDEEL
                     RRPGSARGQRGAEAGQGVQGSPASGAGGCRPGRLLGLMHECKRRPSRSRAVSRGAKTAE
                     TVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWA
                     LTETLRLADHCAGAGGLQGALFTEAVLLSPGAALGASGDSPSPPSSWSCTNSPASSSNS
                     TSPYSCTLSPASPGSDVDYWQPPPPEKHRYAPHLPLARDCI"
     primer_bind     295..316
                     /label=Ngn2_2_fwd
     primer_bind     295..316
                     /label=Ngn2_3_fwd
     primer_bind     complement(1066..1087)
                     /label=Ngn2_1_rev
     primer_bind     complement(1066..1085)
                     /label=Ngn2_2_rev
     primer_bind     complement(1068..1083)
                     /label=Ngn2_3_rev
     primer_bind     1083..1105
                     /label=oKL141
     primer_bind     1084..1106
                     /label=oKL166
     primer_bind     1084..1104
                     /label=P2A_fwd
     CDS             1093..1149
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(1132..1149)
                     /label=P2A_rev
     primer_bind     1146..1167
                     /label=Isl1_2_fwd
     CDS             1150..2196
                     /codon_start=1
                     /product="Isl1(human and mouse seq the same)"
                     /label=Isl1
                     /translation="MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKC
                     AECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIEC
                     FRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPI
                     SARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSP
                     RVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANP
                     VEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPN
                     SMVASPIEA"
     primer_bind     1150..1167
                     /label=Isl1_3_fwd
     primer_bind     1150..1161
                     /label=Isl1_1_fwd
     primer_bind     complement(1562..1589)
                     /label=ISL_part1_rev
     primer_bind     1570..1603
                     /label=ISL1_part2_fwd
     primer_bind     complement(2179..2200)
                     /label=Isl1_2_rev
     primer_bind     complement(2179..2198)
                     /label=Isl1_1_rev
     primer_bind     complement(2181..2196)
                     /label=Isl1_3_rev
     primer_bind     2195..2223
                     /label=T2A_fwd
     CDS             2206..2259
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(2243..2259)
                     /label=T2A_rev
     primer_bind     2256..2278
                     /label=Lhx3_3_fwd
     primer_bind     2257..2278
                     /label=Lhx3_2_fwd
     misc_feature    2260..3459
                     /label=mLhx3
                     /label=Lhx3
                     /note="Lhx3 isoform a from mouse (Mus musculus)"
     primer_bind     2260..2278
                     /label=Lhx3_fwd
     primer_bind     2260..2273
                     /label=Lhx3_part1_fwd
     primer_bind     2260..2272
                     /label=Lhx3_1_fwd
     primer_bind     complement(2827..2860)
                     /label=Lhx3_part1_rev
     primer_bind     2841..2868
                     /label=Lhx3_part2_fwd
     primer_bind     3367..3386
                     /label=oSEQ002 Lhx3_check_for
                     /note="25nm	STD"
     primer_bind     complement(3436..3486)
                     /label=Lhx3_part2_rev
     primer_bind     complement(3439..3459)
                     /label=Lhx3_1_rev
     primer_bind     complement(3439..3459)
                     /label=Lhx3_2_rev
     primer_bind     complement(3442..3486)
                     /label=Lhx3_rev
     primer_bind     complement(3442..3466)
                     /label=Lhx3_3_rev
     primer_bind     complement(3442..3462)
                     /label=Lhx3-WPRE_rev
     primer_bind     3465..3486
                     /label=oGE002 polyA_reporter_bb_fwd
     primer_bind     3467..3482
                     /label=oTA100 GG_entry_upstream_rev
     primer_bind     complement(3467..3482)
                     /label=oTA101 GG_entry_downstream_fwd
     protein_bind    complement(3476..3500)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3517..4105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3683..3702)
                     /label=oSEQ034 WPRE_rev
     primer_bind     3940..3960
                     /label=oSEQ048 phage-dest-fwd-2
     CDS             complement(3988..3999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4102..4119
                     /label=oSEQ013 flipped_WPRE_rev
     primer_bind     complement(4144..4162)
                     /label=oSEQ055 pMXs-rev-seq
     LTR             4285..4754
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(5055..5643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5135..5154)
                     /label=oSEQ067 pBR322-ori_fwd
     CDS             complement(5807..6667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6430..6449
                     /label=oSEQ066 Amp_rev
     primer_bind     complement(6643..6667)
                     /label=oSEQ083 Amp-fwd
     promoter        complement(6668..6772)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6802..7395
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    7458..7815
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7874..8290
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(8068..8087)
                     /label=oSEQ082 pMX-gag-rev
     misc_feature    join(8300..8495,1..179)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
ORIGIN
        1 tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag
       61 gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt
      121 ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagactgc
      181 cggatctagc tagttaatcg acggtatcga taagctatca aacaagtttg tacaaaaaag
      241 caggctccac catgggaacc aattcagtcg actggatccg gtactactgc caccatgttc
      301 gtcaaatctg agactctgga gttgaaggag gaagaggagg tactgatgct gctgggctcg
      361 gcttccccgg cctcggcgac cctgaccccg atgtcctcca gcgcggacga ggaggaggac
      421 gaggagctgc gccggccggg ctccgcgcgt gggcagcgtg gagcggaagc cgggcagggg
      481 gtgcagggca gtccggcgtc gggtgccggg ggttgccggc cagggcggct gctgggcctg
      541 atgcacgagt gcaagcgtcg cccgtcgcgc tcacgggccg tctcccgagg tgccaagacg
      601 gcggagacgg tgcagcgcat caagaagacc cgcaggctca aggccaacaa ccgcgagcgc
      661 aaccgcatgc acaacctaaa cgccgcgctg gacgcgctgc gcgaggtgct gcccaccttc
      721 cccgaggatg ccaagctcac gaagatcgag acgctgcgct tcgcccacaa ttacatctgg
      781 gcgctcaccg agactctgcg cctggcggac cactgcgccg gcgccggtgg cctccagggg
      841 gcgctcttca cggaggcggt gctcctgagc ccgggagctg cgctcggcgc cagcggggac
      901 agcccttctc caccttcctc ctggagctgc accaacagcc cggcgtcatc ctccaactcc
      961 acgtccccat acagctgcac tttatcgccc gctagccccg ggtcagacgt ggactactgg
     1021 cagcccccac ctccggagaa gcatcgttat gcgcctcacc tgcccctcgc cagggactgt
     1081 atcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     1141 cctggaccta tgggagacat gggcgatcca ccaaaaaaaa aacgtctgat ttccctgtgt
     1201 gttggttgcg gcaatcaaat tcacgaccag tatattctga gggtttctcc ggatttggag
     1261 tggcatgcag catgtttgaa atgtgcggag tgtaatcagt atttggacga aagctgtacg
     1321 tgctttgtta gggatgggaa aacctactgt aaaagagatt atatcaggtt gtacgggatc
     1381 aaatgcgcca agtgcagcat aggcttcagc aagaacgact tcgtgatgcg cgcccgctct
     1441 aaggtgtacc acatcgagtg tttccgctgt gtagcctgca gccgacagct catcccggga
     1501 gacgaattcg ccctgcggga ggatgggctt ttctgccgtg cagaccacga tgtggtggag
     1561 agagccagcc tgggagctgg agatcctctc agtcccttgc atccagcgcg gcctctgcaa
     1621 atggcagccg aacccatctc ggctaggcag ccagctctgc ggccgcacgt ccacaagcag
     1681 ccggagaaga ccacccgagt gcggactgtg ctcaacgaga agcagctgca caccttgcgg
     1741 acctgctatg ccgccaaccc tcggccagat gcgctcatga aggagcaact agtggagatg
     1801 acgggcctca gtcccagagt catccgagtg tggtttcaaa acaagcggtg caaggacaag
     1861 aaacgcagca tcatgatgaa gcagctccag cagcagcaac ccaacgacaa aactaatatc
     1921 caggggatga caggaactcc catggtggct gctagtccgg agagacatga tggtggttta
     1981 caggctaacc cagtagaggt gcaaagttac cagccgccct ggaaagtact gagtgacttc
     2041 gccttgcaaa gcgacataga tcagcctgct tttcagcaac tggtcaattt ttcagaagga
     2101 ggaccaggct ctaattctac tggcagtgaa gtagcatcga tgtcctcgca gctcccagat
     2161 acacccaaca gcatggtagc cagtcctatt gaggcaggca gtggtgaggg cagaggaagt
     2221 ctgctaacat gcggtgacgt cgaggagaat cctggcccaa tgctgctaga agcagaactc
     2281 gattgccacc gagagaggcc cggtgcccct ggagcttctg ccctctgtac cttcagcagg
     2341 actccagaga tcccgatgtg tgcaggctgt gaccagcaca tcttggaccg tttcatcctt
     2401 aaggctctgg accgacattg gcacagcaag tgtctcaagt gcagtgactg ccacgtccct
     2461 ctggctgagc gctgcttcag ccgcggggag agcgtctact gcaaagacga cttctttaag
     2521 cgcttcggga ccaagtgcgc cgcatgccag ctgggcatcc cgcccacgca ggtggtgcgc
     2581 cgcgcccagg acttcgtgta ccacctgcat tgcttcgcct gcgtggtctg caagcggcag
     2641 ctggccacgg gcgacgagtt ctacctcatg gaagacagcc ggctggtgtg caaggcggac
     2701 tacgaaacag ccaagcagcg agaagccgag gccacagcca agcggccgcg caccaccatc
     2761 accgccaagc agctggagac gctgaagagc gcctacaaca cttcgcccaa gccggcgcgc
     2821 cacgtgcgcg agcagctctc ctccgagact ggcctggaca tgcgagtggt gcaggtgtgg
     2881 ttccagaatc gccgggctaa ggaaaagaga ctgaagaaag acgctggccg gcagcgctgg
     2941 ggacagtatt tccgcaatat gaagcgctcc cgcggcagtt ccaagtccga caaggacagc
     3001 atccaggagg gacaagacag cgacgccgaa gtctccttca ctgatgagcc gtccatggct
     3061 gacatggggc ctgctaatgg cctgtacagc agcctgggag agcctgcccc tgcgttgggc
     3121 cggcccgtag gaggcctggg cagctttacc ctggatcacg gaggcttgac gggtccagag
     3181 cagtaccgag agctacgccc aggcagcccc tatggcatcc ccccatctcc tgcagccccc
     3241 cagagccttc ctggccccca gcctctcctc tccagcctgg tatacccaga caccaacttg
     3301 agccttgtcc cttcagggcc cccaggtgga cccccaccca tgagggtgct ggctggaaat
     3361 gggcccagct ccgacctgtc cacagagagc agttctggct acccagactt tcctgctagc
     3421 cctgcttcct ggctggatga agtagaccat gctcagttct aacgctgata tctagaccca
     3481 gctttcttgt acaaagtggt tcgatagctt atcgataatc aacctctgga ttacaaaatt
     3541 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3601 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3661 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3721 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3781 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3841 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3901 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3961 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4021 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4081 atctcccttt gggccgcctc cccgcatcga taccgtcgac gataaaataa aagattttat
     4141 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     4201 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     4261 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     4321 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     4381 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     4441 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     4501 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     4561 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     4621 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     4681 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     4741 gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
     4801 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
     4861 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5041 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5281 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5341 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5401 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5461 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5521 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5581 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5641 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5701 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg
     5761 gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6781 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
     6841 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
     6901 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     6961 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
     7021 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     7081 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     7141 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     7201 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
     7261 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     7321 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     7381 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
     7441 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
     7501 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
     7561 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
     7621 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
     7681 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
     7741 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     7801 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     7861 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     7921 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     7981 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     8041 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     8101 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     8161 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     8221 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     8281 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     8341 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     8401 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     8461 actggaccga ccggtggtac ctcaccctta ccgag
//
