LOCUS       Exported               11808 bp ds-DNA     circular SYN 19-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    183..416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             601..645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    943..1060
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1117..1387
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    1117..1387
                     /label=tetracycline response element
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1420..1458
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    1560..1584
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1603..1651
                     /label=Lhx3_fwd
     primer_bind     1603..1646
                     /label=Lhx3_part1_fwd
     primer_bind     1608..1622
                     /label=oTA100 GG_entry_upstream_rev
     primer_bind     complement(1608..1622)
                     /label=oTA101 GG_entry_downstream_fwd
     primer_bind     1623..1645
                     /label=Lhx3_1_fwd
     misc_feature    1627..1635
                     /label=Kozak sequence
                     /note="Partial (missing last G) vertebrate consensus 
                     sequence for strong initiation of translation (Kozak, 
                     1987)"
     primer_bind     1630..1651
                     /label=Lhx3_2_fwd
     primer_bind     1631..1651
                     /label=Lhx3_3_fwd
     misc_feature    1633..2832
                     /label=mLhx3
                     /label=Lhx3
                     /note="Lhx3 isoform a from mouse (Mus musculus)"
     primer_bind     complement(2200..2233)
                     /label=Lhx3_part1_rev
     primer_bind     2214..2241
                     /label=Lhx3_part2_fwd
     primer_bind     2740..2759
                     /label=oSEQ002 Lhx3_check_for
                     /note="25nm	STD"
     primer_bind     complement(2809..2832)
                     /label=Lhx3_part2_rev
     primer_bind     complement(2812..2836)
                     /label=Lhx3_1_rev
     primer_bind     complement(2812..2834)
                     /label=Lhx3_2_rev
     primer_bind     complement(2815..2832)
                     /label=Lhx3_rev
     primer_bind     complement(2815..2832)
                     /label=Lhx3-WPRE_rev
     primer_bind     complement(2815..2832)
                     /label=Lhx3_3_rev
     primer_bind     2832..2854
                     /label=oKL141
     primer_bind     2833..2855
                     /label=oKL166
     primer_bind     2833..2853
                     /label=P2A_fwd
     CDS             2842..2898
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(2881..2898)
                     /label=P2A_rev
     primer_bind     2895..2920
                     /label=Ngn2_2_fwd
     CDS             2899..3687
                     /codon_start=1
                     /product="Mouse Ngn2 "
                     /label=mNgn2
                     /translation="MFVKSETLELKEEEEVLMLLGSASPASATLTPMSSSADEEEDEEL
                     RRPGSARGQRGAEAGQGVQGSPASGAGGCRPGRLLGLMHECKRRPSRSRAVSRGAKTAE
                     TVQRIKKTRRLKANNRERNRMHNLNAALDALREVLPTFPEDAKLTKIETLRFAHNYIWA
                     LTETLRLADHCAGAGGLQGALFTEAVLLSPGAALGASGDSPSPPSSWSCTNSPASSSNS
                     TSPYSCTLSPASPGSDVDYWQPPPPEKHRYAPHLPLARDCI"
     primer_bind     2899..2920
                     /label=Ngn2_3_fwd
     primer_bind     2899..2918
                     /label=gg_N-HA_fwd
     primer_bind     complement(3666..3683)
                     /label=gg_N-HA_rev
     primer_bind     complement(3670..3689)
                     /label=Ngn2_1_rev
     primer_bind     complement(3670..3689)
                     /label=Ngn2_2_rev
     primer_bind     complement(3672..3687)
                     /label=Ngn2_3_rev
     primer_bind     3680..3707
                     /label=gg_HA_fwd
     primer_bind     complement(3688..3703)
                     /label=oFL134
     primer_bind     complement(3709..3726)
                     /label=oFL137
     CDS             3727..3753
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3763..3789
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3799..3825
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3807..3829)
                     /label=Ngn2H_2_rev
     primer_bind     complement(3807..3827)
                     /label=Ngn2H_1_rev
     primer_bind     complement(3807..3825)
                     /label=Ngn2H_3_rev
     primer_bind     3826..3852
                     /label=T2A_fwd
     CDS             3835..3888
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3872..3888)
                     /label=T2A_rev
     primer_bind     3885..3906
                     /label=Isl1_3_fwd
     primer_bind     3886..3906
                     /label=Isl1_2_fwd
     CDS             3889..4935
                     /codon_start=1
                     /product="Isl1(human and mouse seq the same)"
                     /label=Isl1
                     /translation="MGDMGDPPKKKRLISLCVGCGNQIHDQYILRVSPDLEWHAACLKC
                     AECNQYLDESCTCFVRDGKTYCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIEC
                     FRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEPI
                     SARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYAANPRPDALMKEQLVEMTGLSP
                     RVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANP
                     VEVQSYQPPWKVLSDFALQSDIDQPAFQQLVNFSEGGPGSNSTGSEVASMSSQLPDTPN
                     SMVASPIEA"
     primer_bind     complement(4301..4328)
                     /label=ISL_part1_rev
     primer_bind     4309..4342
                     /label=ISL1_part2_fwd
     primer_bind     complement(4918..4935)
                     /label=Isl1_1_rev
     primer_bind     complement(4918..4935)
                     /label=Isl1_2_rev
     primer_bind     complement(4920..4942)
                     /label=Isl1_3_rev
     primer_bind     4941..4962
                     /label=oGE002 polyA_reporter_bb_fwd
     primer_bind     complement(4943..4962)
                     /label=Lhx3_rev
     primer_bind     complement(4943..4962)
                     /label=Lhx3_part2_rev
     primer_bind     4943..4958
                     /label=oTA100 GG_entry_upstream_rev
     primer_bind     complement(4943..4958)
                     /label=oTA101 GG_entry_downstream_fwd
     protein_bind    complement(4952..4976)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5003..5591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             6116..6296
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6328..6552
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6556..6577
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     rep_origin      6598..7026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7040..7369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        7417..7464
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7483..7857
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7987..8108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8157..8173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8181..8197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8205..8235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8250..8271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(8284..8376)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(8559..9147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9318..10178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10179..10283)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        10549..10928
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10930..11128
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             11146..11326
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11373..11498
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     primer_bind     11418..11427
                     /label=gg_N-HA_rev
ORIGIN
        1 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
       61 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      121 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      181 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
      241 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
      301 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
      361 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
      421 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
      481 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
      541 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
      601 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
      661 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
      721 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
      781 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
      841 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
      901 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
      961 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1021 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1081 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     1141 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     1201 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1261 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1321 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1381 atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag
     1441 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     1501 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattatca
     1561 caagtttgta caaaaaagca ggctccacca tgggaaccaa ttcagtcgac tggatccggt
     1621 actactgcca ccatgctgct agaagcagaa ctcgattgcc accgagagag gcccggtgcc
     1681 cctggagctt ctgccctctg taccttcagc aggactccag agatcccgat gtgtgcaggc
     1741 tgtgaccagc acatcttgga ccgtttcatc cttaaggctc tggaccgaca ttggcacagc
     1801 aagtgtctca agtgcagtga ctgccacgtc cctctggctg agcgctgctt cagccgcggg
     1861 gagagcgtct actgcaaaga cgacttcttt aagcgcttcg ggaccaagtg cgccgcatgc
     1921 cagctgggca tcccgcccac gcaggtggtg cgccgcgccc aggacttcgt gtaccacctg
     1981 cattgcttcg cctgcgtggt ctgcaagcgg cagctggcca cgggcgacga gttctacctc
     2041 atggaagaca gccggctggt gtgcaaggcg gactacgaaa cagccaagca gcgagaagcc
     2101 gaggccacag ccaagcggcc gcgcaccacc atcaccgcca agcagctgga gacgctgaag
     2161 agcgcctaca acacttcgcc caagccggcg cgccacgtgc gcgagcagct ctcctccgag
     2221 actggcctgg acatgcgagt ggtgcaggtg tggttccaga atcgccgggc taaggaaaag
     2281 agactgaaga aagacgctgg ccggcagcgc tggggacagt atttccgcaa tatgaagcgc
     2341 tcccgcggca gttccaagtc cgacaaggac agcatccagg agggacaaga cagcgacgcc
     2401 gaagtctcct tcactgatga gccgtccatg gctgacatgg ggcctgctaa tggcctgtac
     2461 agcagcctgg gagagcctgc ccctgcgttg ggccggcccg taggaggcct gggcagcttt
     2521 accctggatc acggaggctt gacgggtcca gagcagtacc gagagctacg cccaggcagc
     2581 ccctatggca tccccccatc tcctgcagcc ccccagagcc ttcctggccc ccagcctctc
     2641 ctctccagcc tggtataccc agacaccaac ttgagccttg tcccttcagg gcccccaggt
     2701 ggacccccac ccatgagggt gctggctgga aatgggccca gctccgacct gtccacagag
     2761 agcagttctg gctacccaga ctttcctgct agccctgctt cctggctgga tgaagtagac
     2821 catgctcagt tcggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     2881 gaggagaacc ctggacctat gttcgtcaaa tctgagactc tggagttgaa ggaggaagag
     2941 gaggtactga tgctgctggg ctcggcttcc ccggcctcgg cgaccctgac cccgatgtcc
     3001 tccagcgcgg acgaggagga ggacgaggag ctgcgccggc cgggctccgc gcgtgggcag
     3061 cgtggagcgg aagccgggca gggggtgcag ggcagtccgg cgtcgggtgc cgggggttgc
     3121 cggccagggc ggctgctggg cctgatgcac gagtgcaagc gtcgcccgtc gcgctcacgg
     3181 gccgtctccc gaggtgccaa gacggcggag acggtgcagc gcatcaagaa gacccgcagg
     3241 ctcaaggcca acaaccgcga gcgcaaccgc atgcacaacc taaacgccgc gctggacgcg
     3301 ctgcgcgagg tgctgcccac cttccccgag gatgccaagc tcacgaagat cgagacgctg
     3361 cgcttcgccc acaattacat ctgggcgctc accgagactc tgcgcctggc ggaccactgc
     3421 gccggcgccg gtggcctcca gggggcgctc ttcacggagg cggtgctcct gagcccggga
     3481 gctgcgctcg gcgccagcgg ggacagccct tctccacctt cctcctggag ctgcaccaac
     3541 agcccggcgt catcctccaa ctccacgtcc ccatacagct gcactttatc gcccgctagc
     3601 cccgggtcag acgtggacta ctggcagccc ccacctccgg agaagcatcg ttatgcgcct
     3661 cacctgcccc tcgccaggga ctgtatcggg tctggctccg gatcaagtgg tggcagcgga
     3721 tcaggatatc catacgatgt accagactat gcgggatcgg gttaccccta cgacgtcccc
     3781 gactacgccg gctccggcta cccctacgac gtccctgatt atgctggcag tggtgagggc
     3841 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat gggagacatg
     3901 ggcgatccac caaaaaaaaa acgtctgatt tccctgtgtg ttggttgcgg caatcaaatt
     3961 cacgaccagt atattctgag ggtttctccg gatttggagt ggcatgcagc atgtttgaaa
     4021 tgtgcggagt gtaatcagta tttggacgaa agctgtacgt gctttgttag ggatgggaaa
     4081 acctactgta aaagagatta tatcaggttg tacgggatca aatgcgccaa gtgcagcata
     4141 ggcttcagca agaacgactt cgtgatgcgc gcccgctcta aggtgtacca catcgagtgt
     4201 ttccgctgtg tagcctgcag ccgacagctc atcccgggag acgaattcgc cctgcgggag
     4261 gatgggcttt tctgccgtgc agaccacgat gtggtggaga gagccagcct gggagctgga
     4321 gatcctctca gtcccttgca tccagcgcgg cctctgcaaa tggcagccga acccatctcg
     4381 gctaggcagc cagctctgcg gccgcacgtc cacaagcagc cggagaagac cacccgagtg
     4441 cggactgtgc tcaacgagaa gcagctgcac accttgcgga cctgctatgc cgccaaccct
     4501 cggccagatg cgctcatgaa ggagcaacta gtggagatga cgggcctcag tcccagagtc
     4561 atccgagtgt ggtttcaaaa caagcggtgc aaggacaaga aacgcagcat catgatgaag
     4621 cagctccagc agcagcaacc caacgacaaa actaatatcc aggggatgac aggaactccc
     4681 atggtggctg ctagtccgga gagacatgat ggtggtttac aggctaaccc agtagaggtg
     4741 caaagttacc agccgccctg gaaagtactg agtgacttcg ccttgcaaag cgacatagat
     4801 cagcctgctt ttcagcaact ggtcaatttt tcagaaggag gaccaggctc taattctact
     4861 ggcagtgaag tagcatcgat gtcctcgcag ctcccagata cacccaacag catggtagcc
     4921 agtcctattg aggcatgacg ctgatatcta gacccagctt tcttgtacaa agtggtgata
     4981 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     5041 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5101 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5161 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5221 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5281 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5341 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     5401 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5461 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5521 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5581 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     5641 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     5701 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     5761 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5821 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     5881 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     5941 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6001 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6061 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6121 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6181 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6241 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     6301 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6361 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6421 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6481 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6541 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6601 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6661 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6721 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6781 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6841 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6901 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6961 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7021 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7081 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7141 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7201 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7261 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7321 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7381 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     7441 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     7501 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     7561 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     7621 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     7681 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     7741 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     7801 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     7861 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7921 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7981 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8041 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8101 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8161 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8221 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8281 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8461 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10261 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    10321 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
    10381 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
    10441 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
    10501 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
    10561 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
    10621 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    10681 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
    10741 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
    10801 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
    10861 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    10921 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
    10981 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
    11041 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
    11101 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
    11161 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    11221 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    11281 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    11341 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    11401 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    11461 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    11521 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    11581 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
    11641 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
    11701 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
    11761 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaa
//
