LOCUS       Exported                9931 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(857..949)
                     /label=PromTet
     rep_origin      complement(1225..1419)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1485..2558)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2987..3616)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3880..3904
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    3977..4930
                     /label=pGmUbi
     protein_bind    4937..5061
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        5086..5116
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        5140..5169
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             5170..5829
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6008..6166
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             6171..6473
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6517..6641)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     terminator      6648..6906
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        6907..7433
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             7444..8154
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      8163..8415
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    8470..8494
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(8617..9205)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    9413..9596
                     /label=TetC term
     CDS             complement(join(9597..9931,1..856))
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
       61 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
      121 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
      181 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
      241 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
      301 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
      361 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
      421 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
      481 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
      541 ccacgatgcg tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      601 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
      661 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
      721 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
      781 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      841 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      901 cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaaa atattgcgct
      961 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagc
     1021 tcggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     1081 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     1141 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     1201 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     1261 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     1321 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     1381 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagaagct ttttcgacct
     1441 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     1501 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     1561 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     1621 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     1681 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     1741 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     1801 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     1861 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     1921 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     1981 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     2041 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     2101 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     2161 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     2221 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     2281 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     2341 cccttgggcg gcttcctaat cgacgacgca ccggctgccg gcggttgccg ggattctttg
     2401 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     2461 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
     2521 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
     2581 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
     2641 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
     2701 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
     2761 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
     2821 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
     2881 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
     2941 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
     3001 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
     3061 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
     3121 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
     3181 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
     3241 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
     3301 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
     3361 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
     3421 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
     3481 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
     3541 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
     3601 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
     3661 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
     3721 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
     3781 accggccagg atttcatgca gccgcacggc tgatgggctg cctgtatcga gtggtgattt
     3841 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     3901 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
     3961 tggatgcagt gggccccact ctgtggaaga caatgccccg cggtccggag taacagataa
     4021 agcgaagctt gaaggtgcat gtgactccgt caagattacg aaaccgccaa ctaccacgca
     4081 aattgcaatt ctcaatttcc tagaaggact ctccgaaaat gcatccaata ccaaatatta
     4141 cccgtgtcat aggcaccaag tgacaccata catgaacacg cgtcacaata tgactggaga
     4201 agggttccac accttatgct ataaaacgcc ccacacccct cctccttcct tcgcagttca
     4261 attccaatat attccattct ctctgtgtat ttccctacct ctcccttcaa ggttagtcga
     4321 tttcttctgt ttttcttctt cgttctttcc atgaattgtg tatgttcttt gatcaatacg
     4381 atgttgattt gattgtgttt tgtttggttt catcgatctt caattttcat aatcagattc
     4441 agcttttatt atctttacaa caacgtcctt aatttgatga ttctttaatc gtagatttgc
     4501 tctaattaga gctttttcat gtcagatccc tttacaacaa gccttaattg ttgattcatt
     4561 aatcgtagat tagggctttt ttcattgatt acttcagatc cgttaaacgt aaccatagat
     4621 cagggctttt tcatgaatta cttcagatcc gttaaacaac agccttattt tttatacttc
     4681 tgtggttttt caagaaattg ttcagatccg ttgacaaaaa gccttattcg ttgattctat
     4741 atcgtttttc gagagatatt gctcagatct gttagcaact gccttgtttg ttgattctat
     4801 tgccgtggat tagggttttt ttcacgagat tgcttcagat ccgtacttaa gattacgtaa
     4861 tggattttga ttctgattta tctgtgattg ttgactcgac agcggccgca cccccggggg
     4921 cgcgccctta ggtaccacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     4981 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     5041 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
     5101 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     5161 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     5221 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     5281 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     5341 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     5401 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     5461 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     5521 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     5581 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     5641 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     5701 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     5761 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     5821 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     5881 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     5941 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     6001 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     6061 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     6121 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     6181 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     6241 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     6301 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     6361 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     6421 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     6481 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     6541 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     6601 tcattttacg tttctcgttc agctttcttg tacaaagtgg tcctaggttc ggttcgagta
     6661 ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt
     6721 tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga
     6781 atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt
     6841 gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt
     6901 caaatcaagg ttccagaagg taattatcca agatgtagca tcaagaatcc aatgtttacg
     6961 ggaaaaacta tggaagtatt atgtgagctc agcaagaagc agatcaatat gcggcacata
     7021 tgcaacctat gttcaaaaat gaagaatgta cagatacaag atcctatact gccagaatac
     7081 gaagaagaat acgtagaaat tgaaaaagaa gaaccaggcg aagaaaagaa tcttgaagac
     7141 gtaagcactg acgacaacaa tgaaaagaag aagataaggt cggtgattgt gaaagagaca
     7201 tagaggacac atgtaaggtg gaaaatgtaa gggcggaaag taaccttatc acaaaggaat
     7261 cttatccccc actacttatc cttttatatt tttccgtgtc atttttgccc ttgagttttc
     7321 ctatataagg aaccaagttc ggcatttgtg aaaacaagaa aaaatttggt gtaagctatt
     7381 ttctttgaag tactgaggat acaacttcag agaaatttgt aagtttgtga tccgcaaaca
     7441 aaaatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     7501 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     7561 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     7621 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     7681 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     7741 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     7801 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     7861 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     7921 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     7981 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     8041 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     8101 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtgagatgtg
     8161 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     8221 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     8281 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     8341 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     8401 ctatgttact agatccgatg ataagctgtc aaacatgagg gacacgaagt ggtttaaact
     8461 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     8521 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgccaa
     8581 ccacaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8641 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8701 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8761 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8821 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8881 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8941 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9001 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9061 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9121 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9181 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9241 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9301 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9361 aacttggtct gacagctaaa acaattcatc cagtaaaata taatcgaata ttatgcgcac
     9421 ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga
     9481 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
     9541 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag
     9601 gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg
     9661 gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc
     9721 gtgacgatca gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga
     9781 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
     9841 ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg
     9901 aacgccagca agacgtagcc cagcgcgtcg g
//
