LOCUS       Exported               10801 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             546..704
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             709..1011
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1055..1179)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     terminator      1189..1447
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        1448..1974
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             1985..2695
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      2704..2956
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    3011..3035
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(3158..3746)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    3954..4137
                     /label=TetC term
     CDS             complement(4138..5328)
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    complement(5329..5421)
                     /label=PromTet
     rep_origin      complement(5696..5890)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(5956..7029)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7458..8087)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8351..8375
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        8481..9384
                     /label=PromGmUbi
     CDS             9439..10209
                     /codon_start=1
                     /label=MiniTurboID
                     /label=birA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.0"
                     /note="/match_length=80.1"
                     /note="/other=CDS"
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    10216..10257
                     /label=V5 tag
     protein_bind    10276..10400
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        10425..10455
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        10479..10508
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             join(10509..10801,1..367)
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
       61 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      121 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
      181 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
      241 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
      301 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
      361 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
      421 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
      481 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
      541 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
      601 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
      661 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
      721 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
      781 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
      841 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
      901 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
      961 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     1021 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     1081 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     1141 attttacgtt tctcgttcag ctttcttgta caaagtggtc ctaggtgatt cggttcgagt
     1201 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     1261 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     1321 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     1381 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     1441 tcaaatcaag gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac
     1501 gggaaaaact atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat
     1561 atgcaaccta tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata
     1621 cgaagaagaa tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga
     1681 cgtaagcact gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac
     1741 atagaggaca catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa
     1801 tcttatcccc cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt
     1861 cctatataag gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat
     1921 tttctttgaa gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaac
     1981 aaaaatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2041 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2101 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2161 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2221 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2281 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2341 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2401 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2461 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2521 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2581 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2641 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagatgt
     2701 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     2761 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     2821 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     2881 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     2941 tctatgttac tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac
     3001 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     3061 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca
     3121 accacaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3901 aaacttggtc tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca
     3961 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
     4021 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     4081 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     4141 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     4201 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     4261 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     4321 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     4381 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     4441 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     4501 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     4561 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     4621 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     4681 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     4741 ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     4801 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     4861 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     4921 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     4981 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcca
     5041 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     5101 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     5161 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     5221 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     5281 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     5341 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
     5401 tcaaacatga gaattcttga aaatattgcg ctgccatttt tggggtgagg ccgttcgcgg
     5461 ccgaggggcg cagcccctgg gggatgggag ctcggtgcct tgatgtgggc gccggcggtc
     5521 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     5581 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     5641 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     5701 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     5761 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     5821 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     5881 gctatccaca ggaaagaagc tttttcgacc tttttcccct gctagggcaa tttgccctag
     5941 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     6001 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     6061 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     6121 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     6181 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     6241 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     6301 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     6361 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     6421 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     6481 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     6541 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     6601 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     6661 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     6721 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     6781 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacgacgc
     6841 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     6901 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     6961 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     7021 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
     7081 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     7141 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     7201 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     7261 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     7321 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     7381 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     7441 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     7501 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     7561 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     7621 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     7681 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     7741 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     7801 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     7861 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     7921 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     7981 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     8041 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     8101 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     8161 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     8221 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
     8281 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
     8341 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     8401 acacattgcg gacgttttta atgtactggg gtggatgcag tgggccccac tctgtggaag
     8461 acaatgcccc gcggtccgga gtaacagata aagcgaagct tgaaggtgca tgtgactccg
     8521 tcaagattac gaaaccgcca actaccacgc aaattgcaat tctcaatttc ctagaaggac
     8581 tctccgaaaa tgcatccaat accaaatatt acccgtgtca taggcaccaa gtgacaccat
     8641 acatgaacac gcgtcacaat atgactggag aagggttcca caccttatgc tataaaacgc
     8701 cccacacccc tcctccttcc ttcgcagttc aattccaata tattccattc tctctgtgta
     8761 tttccctacc tctcccttca aggttagtcg atttcttctg tttttcttct tcgttctttc
     8821 catgaattgt gtatgttctt tgatcaatac gatgttgatt tgattgtgtt ttgtttggtt
     8881 tcatcgatct tcaattttca taatcagatt cagcttttat tatctttaca acaacgtcct
     8941 taatttgatg attctttaat cgtagatttg ctctaattag agctttttca tgtcagatcc
     9001 ctttacaaca agccttaatt gttgattcat taatcgtaga ttagggcttt tttcattgat
     9061 tacttcagat ccgttaaacg taaccataga tcagggcttt ttcatgaatt acttcagatc
     9121 cgttaaacaa cagccttatt ttttatactt ctgtggtttt tcaagaaatt gttcagatcc
     9181 gttgacaaaa agccttattc gttgattcta tatcgttttt cgagagatat tgctcagatc
     9241 tgttagcaac tgccttgttt gttgattcta ttgccgtgga ttagggtttt ttttcacgag
     9301 attgcttcag atccgtactt aagattacgt aatggatttt gattctgatt tatctgtgat
     9361 tgttgactcg acagcggccg cacccccggg ggcgcgccct taacaaaaat ggcaagggac
     9421 cccccagtcg cgaccatgat tcctctgtta aacgcaaagc agattctcgg gcagttggat
     9481 ggggggagtg tggcggtcct gccagtagtc gatagtacta atcaatatct gctagataga
     9541 ataggtgagt taaaaagcgg agacgcgtgc atagccgaat atcaacaagc gggaagaggc
     9601 tcacgaggaa ggaagtggtt tagcccattc ggggcgaatc tgtatctttc tatgttctgg
     9661 cgtctgaaga gaggcccggc agcgattgga ttaggcccgg tcataggcat cgttatggct
     9721 gaggctttaa gaaagctagg tgctgacaaa gtgcgagtga agtggccaaa tgacttatac
     9781 ttgcaagaca ggaagctagc agggatttta gtcgagctag ccggtatcac aggtgacgct
     9841 gcacaaattg ttataggggc cggtatcaat gtggccatgc gtagagtgga agaaagcgtc
     9901 gtgaaccaag gatggatcac tctacaggag gctggaatta atttggatcg aaacacctta
     9961 gctgcgatgc taattagaga actgcgtgcg gcactggagc tgttcgagca ggaaggcttg
    10021 gccccctacc tatcaaggtg ggaaaagctg gataacttca ttaaccgtcc agtgaaattg
    10081 attattggag ataaggaaat ctttggcatc tcacgaggga ttgacaagca aggggcatta
    10141 ctgcttgaac aggatggggt tattaagcct tggatgggag gggaaatctc cttaaggtca
    10201 gcggagaagg cgtccgggaa gccaatccca aatccccttc tcggtcttga cagtaccgga
    10261 ggtggttctg gtaccacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
    10321 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
    10381 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
    10441 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
    10501 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
    10561 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
    10621 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
    10681 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
    10741 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
    10801 a
//
