LOCUS       Exported               10793 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(882..974)
                     /label=PromTet
     rep_origin      complement(1249..1443)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1509..2582)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3011..3640)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3904..3928
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4034..4937
                     /label=PromGmUbi
     protein_bind    4962..5086
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        5111..5141
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        5165..5194
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             5195..5854
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6033..6191
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             6196..6498
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6542..6666)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             6713..7483
                     /codon_start=1
                     /label=MiniTurboID
                     /label=birA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.0"
                     /note="/match_length=80.1"
                     /note="/other=CDS"
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    7490..7531
                     /label=V5 tag
     terminator      7535..7793
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        7794..8320
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             8331..9041
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      9050..9302
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    9357..9381
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(9504..10092)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    10300..10483
                     /label=TetC term
     CDS             complement(join(10484..10793,1..881))
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
       61 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      121 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
      181 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
      241 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
      301 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      361 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
      601 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      661 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
      721 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
      781 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      841 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      901 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      961 tgagaattct tgaaaatatt gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     1021 gcgcagcccc tgggggatgg gagctcggtg ccttgatgtg ggcgccggcg gtcgagtggc
     1081 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     1141 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     1201 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     1261 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     1321 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     1381 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     1441 acaggaaaga agctttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     1501 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     1561 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     1621 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     1681 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     1741 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     1801 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     1861 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     1921 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     1981 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     2041 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     2101 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     2161 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     2221 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     2281 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     2341 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacga cgcaccggct
     2401 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     2461 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     2521 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     2581 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     2641 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     2701 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     2761 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     2821 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     2881 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     2941 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     3001 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     3061 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     3121 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     3181 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     3241 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     3301 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     3361 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     3421 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     3481 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     3541 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     3601 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     3661 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     3721 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     3781 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggctgatgg
     3841 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3901 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     3961 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtgg aagacaatgc
     4021 cccgcggtcc ggagtaacag ataaagcgaa gcttgaaggt gcatgtgact ccgtcaagat
     4081 tacgaaaccg ccaactacca cgcaaattgc aattctcaat ttcctagaag gactctccga
     4141 aaatgcatcc aataccaaat attacccgtg tcataggcac caagtgacac catacatgaa
     4201 cacgcgtcac aatatgactg gagaagggtt ccacacctta tgctataaaa cgccccacac
     4261 ccctcctcct tccttcgcag ttcaattcca atatattcca ttctctctgt gtatttccct
     4321 acctctccct tcaaggttag tcgatttctt ctgtttttct tcttcgttct ttccatgaat
     4381 tgtgtatgtt ctttgatcaa tacgatgttg atttgattgt gttttgtttg gtttcatcga
     4441 tcttcaattt tcataatcag attcagcttt tattatcttt acaacaacgt ccttaatttg
     4501 atgattcttt aatcgtagat ttgctctaat tagagctttt tcatgtcaga tccctttaca
     4561 acaagcctta attgttgatt cattaatcgt agattagggc ttttttcatt gattacttca
     4621 gatccgttaa acgtaaccat agatcagggc tttttcatga attacttcag atccgttaaa
     4681 caacagcctt attttttata cttctgtggt ttttcaagaa attgttcaga tccgttgaca
     4741 aaaagcctta ttcgttgatt ctatatcgtt tttcgagaga tattgctcag atctgttagc
     4801 aactgccttg tttgttgatt ctattgccgt ggattagggt tttttttcac gagattgctt
     4861 cagatccgta cttaagatta cgtaatggat tttgattctg atttatctgt gattgttgac
     4921 tcgacagcgg ccgcaccccc gggggcgcgc ccttaggtac cacaagtttg tacaaaaaag
     4981 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     5041 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     5101 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     5161 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     5221 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     5281 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     5341 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     5401 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     5461 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     5521 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     5581 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     5641 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     5701 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     5761 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     5821 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     5881 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     5941 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     6001 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     6061 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     6121 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     6181 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     6241 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     6301 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     6361 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     6421 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     6481 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     6541 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     6601 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     6661 agtggtccta ggcggaggtg gttctgcaag ggacccccca gtcgcgacca tgattcctct
     6721 gttaaacgca aagcagattc tcgggcagtt ggatgggggg agtgtggcgg tcctgccagt
     6781 agtcgatagt actaatcaat atctgctaga tagaataggt gagttaaaaa gcggagacgc
     6841 gtgcatagcc gaatatcaac aagcgggaag aggctcacga ggaaggaagt ggtttagccc
     6901 attcggggcg aatctgtatc tttctatgtt ctggcgtctg aagagaggcc cggcagcgat
     6961 tggattaggc ccggtcatag gcatcgttat ggctgaggct ttaagaaagc taggtgctga
     7021 caaagtgcga gtgaagtggc caaatgactt atacttgcaa gacaggaagc tagcagggat
     7081 tttagtcgag ctagccggta tcacaggtga cgctgcacaa attgttatag gggccggtat
     7141 caatgtggcc atgcgtagag tggaagaaag cgtcgtgaac caaggatgga tcactctaca
     7201 ggaggctgga attaatttgg atcgaaacac cttagctgcg atgctaatta gagaactgcg
     7261 tgcggcactg gagctgttcg agcaggaagg cttggccccc tacctatcaa ggtgggaaaa
     7321 gctggataac ttcattaacc gtccagtgaa attgattatt ggagataagg aaatctttgg
     7381 catctcacga gggattgaca agcaaggggc attactgctt gaacaggatg gggttattaa
     7441 gccttggatg ggaggggaaa tctccttaag gtcagcggag aaggcgtccg ggaagccaat
     7501 cccaaatccc cttctcggtc ttgacagtac ctgattcggt tcgagtatta tggcattggg
     7561 aaaactgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt
     7621 ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct
     7681 tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt
     7741 gaaattataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcaaggttc
     7801 cagaaggtaa ttatccaaga tgtagcatca agaatccaat gtttacggga aaaactatgg
     7861 aagtattatg tgagctcagc aagaagcaga tcaatatgcg gcacatatgc aacctatgtt
     7921 caaaaatgaa gaatgtacag atacaagatc ctatactgcc agaatacgaa gaagaatacg
     7981 tagaaattga aaaagaagaa ccaggcgaag aaaagaatct tgaagacgta agcactgacg
     8041 acaacaatga aaagaagaag ataaggtcgg tgattgtgaa agagacatag aggacacatg
     8101 taaggtggaa aatgtaaggg cggaaagtaa ccttatcaca aaggaatctt atcccccact
     8161 acttatcctt ttatattttt ccgtgtcatt tttgcccttg agttttccta tataaggaac
     8221 caagttcggc atttgtgaaa acaagaaaaa atttggtgta agctattttc tttgaagtac
     8281 tgaggataca acttcagaga aatttgtaag tttgtgatcc gcaaacaaaa atggtgagca
     8341 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     8401 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     8461 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     8521 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     8581 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     8641 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     8701 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     8761 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     8821 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     8881 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     8941 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     9001 gccgccactc caccggcggc atggacgagc tgtacaagtg agatgtgcag atcgttcaaa
     9061 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     9121 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     9181 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     9241 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     9301 tccgatgata agctgtcaaa catgagggac acgaagtggt ttaaactatc agtgtttgac
     9361 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     9421 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccaacca caggaaccgt
     9481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     9541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     9601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     9661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9721 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9781 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9841 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9901 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     9961 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    10021 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    10081 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    10141 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10201 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    10261 agctaaaaca attcatccag taaaatataa tcgaatatta tgcgcacccg tggccaggac
    10321 ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg
    10381 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
    10441 cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
    10501 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa
    10561 tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg
    10621 gtccaatgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat
    10681 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
    10741 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gcc
//
