LOCUS       Exported               13318 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..13318
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(881..973)
                     /label=PromTet
     rep_origin      complement(1249..1443)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1509..2582)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3011..3640)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3904..3928
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4034..4937
                     /label=PromGmUbi
     protein_bind    4962..5086
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        5121..5151
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        5175..5204
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             5205..5864
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6043..6201
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             6206..6508
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6552..6676)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             6705..6794
                     /codon_start=1
                     /label=3xHA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6819..7077
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        7078..7604
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             7615..11565
                     /label=RUBY
     terminator      11574..11826
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    11881..11905
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(12028..12616)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    12824..13007
                     /label=TetC term
     CDS             complement(join(13008..13318,1..880))
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
       61 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
      121 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
      181 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
      241 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
      301 agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
      361 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
      421 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
      481 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
      541 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc cacaggacgg
      601 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
      661 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
      721 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
      781 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      841 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      901 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
      961 gagaattctt gaaaatattg cgctgccatt tttggggtga ggccgttcgc ggccgagggg
     1021 cgcagcccct ggggggatgg gagctcggtg ccttgatgtg ggcgccggcg gtcgagtggc
     1081 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     1141 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     1201 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     1261 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     1321 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     1381 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     1441 acaggaaaga agctttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     1501 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     1561 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     1621 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     1681 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     1741 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     1801 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     1861 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     1921 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     1981 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     2041 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     2101 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     2161 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     2221 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     2281 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     2341 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacga cgcaccggct
     2401 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     2461 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     2521 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     2581 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     2641 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     2701 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     2761 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     2821 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     2881 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     2941 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     3001 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     3061 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     3121 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     3181 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     3241 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     3301 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     3361 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     3421 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     3481 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     3541 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     3601 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     3661 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     3721 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     3781 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggctgatgg
     3841 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3901 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     3961 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtgg aagacaatgc
     4021 cccgcggtcc ggagtaacag ataaagcgaa gcttgaaggt gcatgtgact ccgtcaagat
     4081 tacgaaaccg ccaactacca cgcaaattgc aattctcaat ttcctagaag gactctccga
     4141 aaatgcatcc aataccaaat attacccgtg tcataggcac caagtgacac catacatgaa
     4201 cacgcgtcac aatatgactg gagaagggtt ccacacctta tgctataaaa cgccccacac
     4261 ccctcctcct tccttcgcag ttcaattcca atatattcca ttctctctgt gtatttccct
     4321 acctctccct tcaaggttag tcgatttctt ctgtttttct tcttcgttct ttccatgaat
     4381 tgtgtatgtt ctttgatcaa tacgatgttg atttgattgt gttttgtttg gtttcatcga
     4441 tcttcaattt tcataatcag attcagcttt tattatcttt acaacaacgt ccttaatttg
     4501 atgattcttt aatcgtagat ttgctctaat tagagctttt tcatgtcaga tccctttaca
     4561 acaagcctta attgttgatt cattaatcgt agattagggc ttttttcatt gattacttca
     4621 gatccgttaa acgtaaccat agatcagggc tttttcatga attacttcag atccgttaaa
     4681 caacagcctt attttttata cttctgtggt ttttcaagaa attgttcaga tccgttgaca
     4741 aaaagcctta ttcgttgatt ctatatcgtt tttcgagaga tattgctcag atctgttagc
     4801 aactgccttg tttgttgatt ctattgccgt ggattagggt tttttttcac gagattgctt
     4861 cagatccgta cttaagatta cgtaatggat tttgattctg atttatctgt gattgttgac
     4921 tcgacagcgg ccgcaccccc gggggcgcgc ccttaggtac cacaagtttg tacaaaaaag
     4981 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     5041 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccctcgaggg
     5101 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
     5161 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     5221 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     5281 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     5341 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     5401 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     5461 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     5521 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     5581 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     5641 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     5701 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     5761 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     5821 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     5881 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     5941 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     6001 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     6061 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     6121 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     6181 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     6241 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     6301 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     6361 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     6421 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     6481 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     6541 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     6601 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     6661 tcttgtacaa agtggttgat aacagcgggt taattaacat cttttaccca tacgatgttc
     6721 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg
     6781 ttccagatta cgctgctcag tgcagcgctt agcctaggtt cggttcgagt attatggcat
     6841 tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc
     6901 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg
     6961 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga
     7021 atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcaag
     7081 gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac gggaaaaact
     7141 atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat atgcaaccta
     7201 tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata cgaagaagaa
     7261 tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga cgtaagcact
     7321 gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac atagaggaca
     7381 catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa tcttatcccc
     7441 cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt cctatataag
     7501 gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat tttctttgaa
     7561 gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaac aaaaatggat
     7621 catgcgaccc tcgccatgat cctcgcgatc tggttcatca gcttccactt catcaagctg
     7681 ctgttctccc agcagaccac caagctgctt ccgccaggac caaagccgct tccgatcatc
     7741 ggcaacatcc ttgaggtggg caagaagccg catcggtcct tcgccaacct cgccaagatt
     7801 cacggcccac tcatttccct cagactcggc tctgtgacca ccatcgttgt gtcctctgcc
     7861 gacgtggcca aagagatgtt cctcaagaag gatcacccgc tctccaaccg cacgatcccg
     7921 aatagtgtta cagccggcga ccaccacaag ctcaccatgt cttggctccc ggtgtctccg
     7981 aagtggcgca acttccgcaa gattaccgcc gtgcatctgc tctccccaca gagactcgat
     8041 gcctgccaga cattcaggca cgccaaggtg cagcagctct acgagtacgt tcaagagtgc
     8101 gcccagaaag gccaggccgt ggatattggc aaggccgcct ttacgaccag cctcaacctc
     8161 ctcagcaagc tgttcttcag cgtcgagctg gcgcaccaca agtcccatac cagccaagag
     8221 ttcaaagagc tgatctggaa catcatggaa gatataggca agccgaacta cgccgactac
     8281 ttcccgattc tcggctgcgt tgacccatct ggcattagaa gaaggctcgc ctgctccttc
     8341 gacaagctga tcgccgtgtt ccagggcatc atctgcgaga gactcgcccc agattcctcc
     8401 accacaacta ccaccaccac cgacgacgtg ctcgatgtgc tcctccagct gttcaagcag
     8461 aacgagctga cgatgggcga gatcaaccac ctcctcgtgg acatcttcga cgccggcacc
     8521 gataccacat cctccacatt cgagtgggtg atgaccgagc tgatccgcaa tccagagatg
     8581 atggaaaagg cccaagagga aatcaagcag gtcctcggca aggacaagca gatccaagag
     8641 tccgacatca tcaacctgcc gtacctccag gcgatcatca aagagacact ccgcctccat
     8701 ccgccgaccg tgttcttgct cccaagaaag gccgacaccg atgtcgagct gtacggctac
     8761 atcgtgccga aggatgccca gatcctcgtg aacctctggg ccattggcag ggacccaaac
     8821 gcctggcaga acgccgatat tttcagccca gagcgcttca tcggctgcga gatcgatgtt
     8881 aagggccgcg atttcggcct ccttccattt ggcgctggcc gcagaatttg cccaggcatg
     8941 aatctcgcca tcaggatgct caccctcatg ctcgccacac tcctccagtt cttcaactgg
     9001 aagctcgaag gcgacatctc cccgaaggac ctcgacatgg acgagaagtt cggcattgcg
     9061 ctccaaaaga ccaagccgct caagctcatc ccgattccgc gctacggtag cggagctacc
     9121 aattttagcc tccttaagca ggcaggtgat gtagaagaga accccgggcc tatgaagatg
     9181 atgaacggcg aggacgccaa cgaccagatg atcaaagagt ccttcttcat cacccacggc
     9241 aacccgatcc tcaccgtcga ggatacacat ccgctcaggc cgttcttcga gacatggcgc
     9301 gagaagattt tctccaagaa gccgaaggcc atcctcatca tctccggcca ctgggagaca
     9361 gtgaagccaa ccgtgaacgc cgtgcacatc aacgacacca tccacgactt cgacgactac
     9421 ccagccgcca tgtaccagtt caagtaccca gctccaggcg agccagagct tgcgagaaag
     9481 gtggaagaga tcctcaagaa gtccgggttc gagacagccg agacagacca aaagaggggc
     9541 cttgatcacg gcgcctgggt tccactcatg ctcatgtatc cagaggcgga catcccggtg
     9601 tgccagctct cagttcagcc acatctcgac ggcacctacc actacaatct cggcagagcc
     9661 ctcgcgccgc tcaagaatga tggcgtgctc attattggct ccggcagcgc cacacatcca
     9721 ctcgatgaga caccgcacta cttcgatggt gttgcccctt gggccgctgc cttcgattct
     9781 tggcttagga aggccctcat caacggccgc ttcgaggaag tgaacatcta cgagagcaag
     9841 gccccgaact ggaagctcgc ccatccattt ccagagcact tctacccgct ccacgttgtg
     9901 ctcggcgctg ctggtgaaaa gtggaaggcc gagctgatcc actcctcctg ggatcatggc
     9961 acactttgcc acggctccta caagttcacc tccgccggat ccggagcaac caactttagc
    10021 ctgctcaagc aagcaggaga tgttgaggaa aatcctggcc ccatgaccgc catcaagatg
    10081 aacaccaacg gcgagggcga gacacagcac atcctcatga tcccgttcat ggcgcagggc
    10141 cacctcaggc catttctcga actcgccatg ttcctctaca agcgctccca cgtgatcatc
    10201 accctgctca caactccgct caatgccggc ttcctcaggc acctccttca ccaccattcc
    10261 tactcctcca gcggcatcag gatcgtcgag ctgccattca actccaccaa ccacggactc
    10321 ccaccgggca tcgagaacac cgataagctc acactcccgc tcgtggtgtc cctcttccat
    10381 tccaccatca gcctcgatcc gcacctccgc gattacatct ccaggcattt cagcccagcc
    10441 aggccaccac tctgcgtgat ccatgatgtg ttcctcggct gggttgacca ggtggccaag
    10501 gatgtgggct ctacaggcgt ggtgttcaca acaggcggcg cttatggcac atccgcctac
    10561 gtgtccatct ggaacgatct cccgcaccag aactactccg acgaccaaga gttcccgctg
    10621 ccaggcttcc cagagaacca taagttccgc aggtcccagc tccatcggtt cctcagatat
    10681 gccgacggct ccgacgattg gtccaagtat ttccagccgc agctccgcca gtccatgaag
    10741 tcttttggct ggctctgcaa ctccgtggaa gagatcgaga cactcggctt ctccatcctc
    10801 cgcaactaca ccaagctgcc gatctggggc atcggcccac ttattgcttc cccagtgcag
    10861 cactcctcct ccgacaacaa ttcaacaggc gccgagttcg tgcagtggct cagcctcaaa
    10921 gagccggact ccgtcctcta catctccttc ggctcccaga acacgatcag cccgacgcag
    10981 atgatggaac tcgctgctgg ccttgagtcc tccgagaagc cattcctctg ggtgatcaga
    11041 gccccgttcg gcttcgacat caacgaagag atgcgcccag agtggctgcc agagggcttt
    11101 gaggaacgca tgaaggtgaa gaaacagggc aagctcgtgt acaagctcgg cccgcagctt
    11161 gagatcctca accatgaatc catcggcggc tttctcaccc actgcggatg gaacagcatc
    11221 cttgagtctc ttcgcgaggg cgttccgatg cttggatggc cacttgctgc cgagcaggcc
    11281 tacaacctca agtacctcga agatgagatg ggcgtcgcgg ttgagcttgc tagaggcctc
    11341 gaaggcgaga tctccaaaga gaaggtcaag cgcatcgtcg agatgatcct tgagcgcaac
    11401 gagggctcca aaggctggga gatgaagaat cgcgccgtgg aaatgggcaa aaagctcaag
    11461 gacgccgtga acgaggaaaa agagctgaag ggctcctccg tgaaggcgat cgacgatttc
    11521 ctcgacgccg tcatgcaggc caaacttgag ccaagcctcc agtgagatgt gcagatcgtt
    11581 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
    11641 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
    11701 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
    11761 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
    11821 tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac tatcagtgtt
    11881 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
    11941 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca accacaggaa
    12001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    12061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    12121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    12181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    12241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    12301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    12361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    12481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12781 tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca cccgtggcca
    12841 ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
    12901 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
    12961 aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
    13021 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct
    13081 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc
    13141 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc
    13201 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
    13261 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgcca
//
