LOCUS       Exported               13899 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(882..974)
                     /label=PromTet
     misc_feature    1250..1444
                     /label=pVS1 oriV
     CDS             complement(1510..2583)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3012..3641)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3905..3929
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4035..4938
                     /label=PromGmUbi
     protein_bind    4963..5087
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        5112..5142
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        5166..5195
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             5196..5855
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6034..6192
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             6197..6499
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6543..6667)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             6675..7391
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      7401..7659
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        7660..8186
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             8197..12147
                     /label=RUBY
     terminator      12156..12408
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    12463..12487
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(12610..13198)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    13406..13589
                     /label=TetC term
     CDS             complement(join(13590..13899,1..881))
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
       61 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      121 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
      181 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
      241 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
      301 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      361 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
      601 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      661 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
      721 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
      781 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      841 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      901 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      961 tgagaattct tgaaaatatt gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     1021 gcgcagcccc tggggggatg ggagctcggt gccttgatgt gggcgccggc ggtcgagtgg
     1081 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     1141 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     1201 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     1261 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     1321 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     1381 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     1441 cacaggaaag aagctttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     1501 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     1561 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     1621 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     1681 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     1741 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     1801 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     1861 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     1921 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     1981 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     2041 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     2101 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     2161 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     2221 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     2281 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     2341 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg acgcaccggc
     2401 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     2461 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     2521 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     2581 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     2641 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     2701 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     2761 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     2821 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     2881 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     2941 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     3001 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     3061 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     3121 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     3181 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     3241 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     3301 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     3361 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     3421 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     3481 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     3541 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     3601 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     3661 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     3721 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     3781 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggctgatg
     3841 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     3901 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     3961 tgcggacgtt tttaatgtac tggggtggat gcagtgggcc ccactctgtg gaagacaatg
     4021 ccccgcggtc cggagtaaca gataaagcga agcttgaagg tgcatgtgac tccgtcaaga
     4081 ttacgaaacc gccaactacc acgcaaattg caattctcaa tttcctagaa ggactctccg
     4141 aaaatgcatc caataccaaa tattacccgt gtcataggca ccaagtgaca ccatacatga
     4201 acacgcgtca caatatgact ggagaagggt tccacacctt atgctataaa acgccccaca
     4261 cccctcctcc ttccttcgca gttcaattcc aatatattcc attctctctg tgtatttccc
     4321 tacctctccc ttcaaggtta gtcgatttct tctgtttttc ttcttcgttc tttccatgaa
     4381 ttgtgtatgt tctttgatca atacgatgtt gatttgattg tgttttgttt ggtttcatcg
     4441 atcttcaatt ttcataatca gattcagctt ttattatctt tacaacaacg tccttaattt
     4501 gatgattctt taatcgtaga tttgctctaa ttagagcttt ttcatgtcag atccctttac
     4561 aacaagcctt aattgttgat tcattaatcg tagattaggg cttttttcat tgattacttc
     4621 agatccgtta aacgtaacca tagatcaggg ctttttcatg aattacttca gatccgttaa
     4681 acaacagcct tattttttat acttctgtgg tttttcaaga aattgttcag atccgttgac
     4741 aaaaagcctt attcgttgat tctatatcgt ttttcgagag atattgctca gatctgttag
     4801 caactgcctt gtttgttgat tctattgccg tggattaggg ttttttttca cgagattgct
     4861 tcagatccgt acttaagatt acgtaatgga ttttgattct gatttatctg tgattgttga
     4921 ctcgacagcg gccgcacccc cgggggcgcg cccttaggta ccacaagttt gtacaaaaaa
     4981 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     5041 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     5101 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     5161 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     5221 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     5281 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     5341 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     5401 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     5461 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     5521 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     5581 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     5641 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     5701 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     5761 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     5821 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     5881 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     5941 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     6001 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     6061 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     6121 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     6181 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     6241 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     6301 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     6361 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     6421 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     6481 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     6541 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     6601 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     6661 aagtggtgat atcaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6841 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     6901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7081 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     7141 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     7201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     7261 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     7321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     7381 agctgtacaa gtaacctagg ttcggttcga gtattatggc attgggaaaa ctgtttttct
     7441 tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact
     7501 gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca
     7561 aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga
     7621 tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatca aggttccaga aggtaattat
     7681 ccaagatgta gcatcaagaa tccaatgttt acgggaaaaa ctatggaagt attatgtgag
     7741 ctcagcaaga agcagatcaa tatgcggcac atatgcaacc tatgttcaaa aatgaagaat
     7801 gtacagatac aagatcctat actgccagaa tacgaagaag aatacgtaga aattgaaaaa
     7861 gaagaaccag gcgaagaaaa gaatcttgaa gacgtaagca ctgacgacaa caatgaaaag
     7921 aagaagataa ggtcggtgat tgtgaaagag acatagagga cacatgtaag gtggaaaatg
     7981 taagggcgga aagtaacctt atcacaaagg aatcttatcc cccactactt atccttttat
     8041 atttttccgt gtcatttttg cccttgagtt ttcctatata aggaaccaag ttcggcattt
     8101 gtgaaaacaa gaaaaaattt ggtgtaagct attttctttg aagtactgag gatacaactt
     8161 cagagaaatt tgtaagtttg tgatccgcaa acaaaaatgg atcatgcgac cctcgccatg
     8221 atcctcgcga tctggttcat cagcttccac ttcatcaagc tgctgttctc ccagcagacc
     8281 accaagctgc ttccgccagg accaaagccg cttccgatca tcggcaacat ccttgaggtg
     8341 ggcaagaagc cgcatcggtc cttcgccaac ctcgccaaga ttcacggccc actcatttcc
     8401 ctcagactcg gctctgtgac caccatcgtt gtgtcctctg ccgacgtggc caaagagatg
     8461 ttcctcaaga aggatcaccc gctctccaac cgcacgatcc cgaatagtgt tacagccggc
     8521 gaccaccaca agctcaccat gtcttggctc ccggtgtctc cgaagtggcg caacttccgc
     8581 aagattaccg ccgtgcatct gctctcccca cagagactcg atgcctgcca gacattcagg
     8641 cacgccaagg tgcagcagct ctacgagtac gttcaagagt gcgcccagaa aggccaggcc
     8701 gtggatattg gcaaggccgc ctttacgacc agcctcaacc tcctcagcaa gctgttcttc
     8761 agcgtcgagc tggcgcacca caagtcccat accagccaag agttcaaaga gctgatctgg
     8821 aacatcatgg aagatatagg caagccgaac tacgccgact acttcccgat tctcggctgc
     8881 gttgacccat ctggcattag aagaaggctc gcctgctcct tcgacaagct gatcgccgtg
     8941 ttccagggca tcatctgcga gagactcgcc ccagattcct ccaccacaac taccaccacc
     9001 accgacgacg tgctcgatgt gctcctccag ctgttcaagc agaacgagct gacgatgggc
     9061 gagatcaacc acctcctcgt ggacatcttc gacgccggca ccgataccac atcctccaca
     9121 ttcgagtggg tgatgaccga gctgatccgc aatccagaga tgatggaaaa ggcccaagag
     9181 gaaatcaagc aggtcctcgg caaggacaag cagatccaag agtccgacat catcaacctg
     9241 ccgtacctcc aggcgatcat caaagagaca ctccgcctcc atccgccgac cgtgttcttg
     9301 ctcccaagaa aggccgacac cgatgtcgag ctgtacggct acatcgtgcc gaaggatgcc
     9361 cagatcctcg tgaacctctg ggccattggc agggacccaa acgcctggca gaacgccgat
     9421 attttcagcc cagagcgctt catcggctgc gagatcgatg ttaagggccg cgatttcggc
     9481 ctccttccat ttggcgctgg ccgcagaatt tgcccaggca tgaatctcgc catcaggatg
     9541 ctcaccctca tgctcgccac actcctccag ttcttcaact ggaagctcga aggcgacatc
     9601 tccccgaagg acctcgacat ggacgagaag ttcggcattg cgctccaaaa gaccaagccg
     9661 ctcaagctca tcccgattcc gcgctacggt agcggagcta ccaattttag cctccttaag
     9721 caggcaggtg atgtagaaga gaaccccggg cctatgaaga tgatgaacgg cgaggacgcc
     9781 aacgaccaga tgatcaaaga gtccttcttc atcacccacg gcaacccgat cctcaccgtc
     9841 gaggatacac atccgctcag gccgttcttc gagacatggc gcgagaagat tttctccaag
     9901 aagccgaagg ccatcctcat catctccggc cactgggaga cagtgaagcc aaccgtgaac
     9961 gccgtgcaca tcaacgacac catccacgac ttcgacgact acccagccgc catgtaccag
    10021 ttcaagtacc cagctccagg cgagccagag cttgcgagaa aggtggaaga gatcctcaag
    10081 aagtccgggt tcgagacagc cgagacagac caaaagaggg gccttgatca cggcgcctgg
    10141 gttccactca tgctcatgta tccagaggcg gacatcccgg tgtgccagct ctcagttcag
    10201 ccacatctcg acggcaccta ccactacaat ctcggcagag ccctcgcgcc gctcaagaat
    10261 gatggcgtgc tcattattgg ctccggcagc gccacacatc cactcgatga gacaccgcac
    10321 tacttcgatg gtgttgcccc ttgggccgct gccttcgatt cttggcttag gaaggccctc
    10381 atcaacggcc gcttcgagga agtgaacatc tacgagagca aggccccgaa ctggaagctc
    10441 gcccatccat ttccagagca cttctacccg ctccacgttg tgctcggcgc tgctggtgaa
    10501 aagtggaagg ccgagctgat ccactcctcc tgggatcatg gcacactttg ccacggctcc
    10561 tacaagttca cctccgccgg atccggagca accaacttta gcctgctcaa gcaagcagga
    10621 gatgttgagg aaaatcctgg ccccatgacc gccatcaaga tgaacaccaa cggcgagggc
    10681 gagacacagc acatcctcat gatcccgttc atggcgcagg gccacctcag gccatttctc
    10741 gaactcgcca tgttcctcta caagcgctcc cacgtgatca tcaccctgct cacaactccg
    10801 ctcaatgccg gcttcctcag gcacctcctt caccaccatt cctactcctc cagcggcatc
    10861 aggatcgtcg agctgccatt caactccacc aaccacggac tcccaccggg catcgagaac
    10921 accgataagc tcacactccc gctcgtggtg tccctcttcc attccaccat cagcctcgat
    10981 ccgcacctcc gcgattacat ctccaggcat ttcagcccag ccaggccacc actctgcgtg
    11041 atccatgatg tgttcctcgg ctgggttgac caggtggcca aggatgtggg ctctacaggc
    11101 gtggtgttca caacaggcgg cgcttatggc acatccgcct acgtgtccat ctggaacgat
    11161 ctcccgcacc agaactactc cgacgaccaa gagttcccgc tgccaggctt cccagagaac
    11221 cataagttcc gcaggtccca gctccatcgg ttcctcagat atgccgacgg ctccgacgat
    11281 tggtccaagt atttccagcc gcagctccgc cagtccatga agtcttttgg ctggctctgc
    11341 aactccgtgg aagagatcga gacactcggc ttctccatcc tccgcaacta caccaagctg
    11401 ccgatctggg gcatcggccc acttattgct tccccagtgc agcactcctc ctccgacaac
    11461 aattcaacag gcgccgagtt cgtgcagtgg ctcagcctca aagagccgga ctccgtcctc
    11521 tacatctcct tcggctccca gaacacgatc agcccgacgc agatgatgga actcgctgct
    11581 ggccttgagt cctccgagaa gccattcctc tgggtgatca gagccccgtt cggcttcgac
    11641 atcaacgaag agatgcgccc agagtggctg ccagagggct ttgaggaacg catgaaggtg
    11701 aagaaacagg gcaagctcgt gtacaagctc ggcccgcagc ttgagatcct caaccatgaa
    11761 tccatcggcg gctttctcac ccactgcgga tggaacagca tccttgagtc tcttcgcgag
    11821 ggcgttccga tgcttggatg gccacttgct gccgagcagg cctacaacct caagtacctc
    11881 gaagatgaga tgggcgtcgc ggttgagctt gctagaggcc tcgaaggcga gatctccaaa
    11941 gagaaggtca agcgcatcgt cgagatgatc cttgagcgca acgagggctc caaaggctgg
    12001 gagatgaaga atcgcgccgt ggaaatgggc aaaaagctca aggacgccgt gaacgaggaa
    12061 aaagagctga agggctcctc cgtgaaggcg atcgacgatt tcctcgacgc cgtcatgcag
    12121 gccaaacttg agccaagcct ccagtgagat gtgcagatcg ttcaaacatt tggcaataaa
    12181 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
    12241 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
    12301 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
    12361 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatccg atgataagct
    12421 gtcaaacatg agggacacga agtggtttaa actatcagtg tttgacagga tatattggcg
    12481 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
    12541 aggtttatcc gttcgtccat ttgtatgtgc caaccacagg aaccgtaaaa aggccgcgtt
    12601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    12661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    12721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    12781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    12841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    12901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    12961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    13021 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    13081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    13141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    13201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    13261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    13321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct aaaacaattc
    13381 atccagtaaa atataatcga atattatgcg cacccgtggc caggacccaa cgctgcccga
    13441 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
    13501 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
    13561 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
    13621 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
    13681 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
    13741 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
    13801 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
    13861 ataatgggga aggccatcca gcctcgcgtc gcgaacgcc
//
