LOCUS       Exported               12235 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             555..713
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             718..1020
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1064..1188)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     terminator      1195..1453
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        1454..1980
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             1991..2701
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      2710..2962
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    3017..3041
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(3164..3752)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    3960..4143
                     /label=TetC term
     CDS             complement(4144..5334)
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    complement(5335..5427)
                     /label=PromTet
     rep_origin      complement(5703..5897)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(5963..7036)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7465..8094)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8358..8382
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        8486..9389
                     /label=PromGmUbi
     CDS             9410..9627
                     /codon_start=1
                     /label=GAL4 DNA binding domain (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=49.4"
                     /note="/other=CDS"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIF"
     CDS             9638..9871
                     /codon_start=1
                     /label=VP16 AD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             9878..10708
                     /codon_start=1
                     /label=GVG
                     /label=Nr3c1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=34.8"
                     /note="/other=CDS"
                     /translation="IQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLY
                     AGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLM
                     AFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEY
                     LCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLL
                     DSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
     terminator      10738..11034
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=46.3"
                     /note="/other=terminator"
     terminator      11163..11221
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=9.2"
                     /note="/other=terminator"
     protein_bind    11314..11331
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    11332..11349
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    11350..11368
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     protein_bind    11381..11398
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    11399..11416
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    11417..11435
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     promoter        11482..11528
                     /label=minimal CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             11596..11685
                     /codon_start=1
                     /label=3xHA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    11707..11831
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        11868..11898
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        11922..11951
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             join(11952..12235,1..376)
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
       61 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      121 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
      181 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
      241 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
      301 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
      361 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
      421 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
      481 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
      541 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
      601 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
      661 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
      721 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
      781 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
      841 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
      901 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
      961 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     1021 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     1081 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     1141 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtcc taggttcggt
     1201 tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
     1261 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
     1321 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
     1381 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
     1441 aatgtgtcaa atcaaggttc cagaaggtaa ttatccaaga tgtagcatca agaatccaat
     1501 gtttacggga aaaactatgg aagtattatg tgagctcagc aagaagcaga tcaatatgcg
     1561 gcacatatgc aacctatgtt caaaaatgaa gaatgtacag atacaagatc ctatactgcc
     1621 agaatacgaa gaagaatacg tagaaattga aaaagaagaa ccaggcgaag aaaagaatct
     1681 tgaagacgta agcactgacg acaacaatga aaagaagaag ataaggtcgg tgattgtgaa
     1741 agagacatag aggacacatg taaggtggaa aatgtaaggg cggaaagtaa ccttatcaca
     1801 aaggaatctt atcccccact acttatcctt ttatattttt ccgtgtcatt tttgcccttg
     1861 agttttccta tataaggaac caagttcggc atttgtgaaa acaagaaaaa atttggtgta
     1921 agctattttc tttgaagtac tgaggataca acttcagaga aatttgtaag tttgtgatcc
     1981 gcaaacaaaa atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     2041 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     2101 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     2161 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     2221 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     2281 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     2341 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     2401 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     2461 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     2521 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     2581 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     2641 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtg
     2701 agatgtgcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     2761 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     2821 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2881 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     2941 gtgtcatcta tgttactaga tccgatgata agctgtcaaa catgagggac acgaagtggt
     3001 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     3061 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     3121 gtgccaacca caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3601 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3901 atgagtaaac ttggtctgac agctaaaaca attcatccag taaaatataa tcgaatatta
     3961 tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga
     4021 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     4081 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
     4141 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     4201 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
     4261 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
     4321 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     4381 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     4441 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
     4501 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     4561 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     4621 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     4681 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     4741 gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa
     4801 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     4861 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     4921 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     4981 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     5041 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     5101 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     5161 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     5221 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     5281 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     5341 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat
     5401 aagctgtcaa acatgagaat tcttgaaaat attgcgctgc catttttggg gtgaggccgt
     5461 tcgcggccga ggggcgcagc ccctgggggg atgggagctc ggtgccttga tgtgggcgcc
     5521 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     5581 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     5641 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     5701 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     5761 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     5821 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     5881 tgtacgtgct atccacagga aagaagcttt ttcgaccttt ttcccctgct agggcaattt
     5941 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     6001 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     6061 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     6121 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     6181 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     6241 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     6301 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     6361 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     6421 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     6481 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     6541 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     6601 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     6661 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     6721 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     6781 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     6841 acgacgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     6901 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     6961 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     7021 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     7081 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     7141 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     7201 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     7261 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     7321 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     7381 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     7441 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     7501 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     7561 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     7621 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     7681 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     7741 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     7801 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     7861 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     7921 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
     7981 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
     8041 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
     8101 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
     8161 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
     8221 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
     8281 cgcacggctg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     8341 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
     8401 cttaataaca cattgcggac gtttttaatg tactggggtg gatgcagtgg gcgggcccca
     8461 ctctgtggaa gacaatgcct ccggagtaac agataaagcg aagcttgaag gtgcatgtga
     8521 ctccgtcaag attacgaaac cgccaactac cacgcaaatt gcaattctca atttcctaga
     8581 aggactctcc gaaaatgcat ccaataccaa atattacccg tgtcataggc accaagtgac
     8641 accatacatg aacacgcgtc acaatatgac tggagaaggg ttccacacct tatgctataa
     8701 aacgccccac acccctcctc cttccttcgc agttcaattc caatatattc cattctctct
     8761 gtgtatttcc ctacctctcc cttcaaggtt agtcgatttc ttctgttttt cttcttcgtt
     8821 ctttccatga attgtgtatg ttctttgatc aatacgatgt tgatttgatt gtgttttgtt
     8881 tggtttcatc gatcttcaat tttcataatc agattcagct tttattatct ttacaacaac
     8941 gtccttaatt tgatgattct ttaatcgtag atttgctcta attagagctt tttcatgtca
     9001 gatcccttta caacaagcct taattgttga ttcattaatc gtagattagg gcttttttca
     9061 ttgattactt cagatccgtt aaacgtaacc atagatcagg gctttttcat gaattacttc
     9121 agatccgtta aacaacagcc ttatttttta tacttctgtg gtttttcaag aaattgttca
     9181 gatccgttga caaaaagcct tattcgttga ttctatatcg tttttcgaga gatattgctc
     9241 agatctgtta gcaactgcct tgtttgttga ttctattgcc gtggattagg gttttttttc
     9301 acgagattgc ttcagatccg tacttaagat tacgtaatgg attttgattc tgatttatct
     9361 gtgattgttg actcgacagc ggccgcaccg gcgcgccatg gcagatccaa tgaagctact
     9421 gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga
     9481 aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac
     9541 caaaagatct ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact
     9601 ggaacagcta tttctactga tttttccaag gtcgagcgcc cccccgaccg atgtcagcct
     9661 gggggacgag ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga
     9721 cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca
     9781 cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac
     9841 cgatgccctt ggaattgacg agtacggtgg ggatccaatt cagcaagcca ctgcaggagt
     9901 ctcacaagac acttcggaaa atcctaacaa aacaatagtt cctgctgcat taccacagct
     9961 cacccctacc ttggtgtcac tgctggaggt gattgaaccc gaggtgttgt atgcaggata
    10021 tgatagctct gttccagatt cagcatggag aattatgacc acactcaaca tgttaggtgg
    10081 gcgtcaagtg attgcagcag tgaaatgggc aaaggcgata ccaggcttca gaaacttaca
    10141 cctggatgac caaatgaccc tgctacagta ctcatggatg tttctcatgg catttgccct
    10201 gggttggaga tcatacagac aatcaagtgg aaacctgctc tgctttgctc ctgatctgat
    10261 tattaatgag cagagaatgt ctctaccctg catgtatgac caatgtaaac acatgctgtt
    10321 tgtctcctct gaattacaaa gattgcaggt atcctatgaa gagtatctct gtatgaaaac
    10381 cttactgctt ctctcctcag ttcctaagga aggtctgaag agccaagagt tatttgatga
    10441 gattcgaatg acttatatca aagagctagg aaaagccatc gtcaaaaggg aagggaactc
    10501 cagtcagaac tggcaacggt tttaccaact gacaaagctt ctggactcca tgcatgaggt
    10561 ggttgagaat ctccttacct actgcttcca gacatttttg gataagacca tgagtattga
    10621 attcccagag atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa
    10681 tatcaaaaag cttctgtttc atcaaaaatg actcgaccta actgagtaag ctagcttgtt
    10741 cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta
    10801 ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt
    10861 taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta
    10921 tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa
    10981 atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactagatcc
    11041 ccaaacaagc ttgaaactga aggcgggaaa cgacaatctg atcatgagcg gagaattaag
    11101 ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga
    11161 catatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca acatgcatca
    11221 atccgcggtt atgactctct taagagagtc atgaattcga gcgggaattg atcccccctc
    11281 gacagcttgc atgccggtcg actctagagg atccgggtga cagccctccg acgggtgaca
    11341 gccctccgac gggtgacagc cctccgaatt ctagaggatc cgggtgacag ccctccgacg
    11401 ggtgacagcc ctccgacggg tgacagccct ccgaattcga gctcagtacc cggggatctg
    11461 tcgacctcga tcgagatctt cgcaagaccc ttcctctata taaggaagtt catttcattt
    11521 ggagaggaca cgctgaagct agtcgactct agcctcgaat ccttaggtac catgagcggg
    11581 ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc
    11641 ccggactatg caggatccta tccatatgac gttccagatt acgctgctca gtgcagcgct
    11701 gttatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
    11761 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
    11821 cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt acactttatg
    11881 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
    11941 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
    12001 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
    12061 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
    12121 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
    12181 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gtttt
//
