LOCUS       Exported               12230 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(881..973)
                     /label=PromTet
     rep_origin      complement(1249..1443)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1509..2582)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3011..3640)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3904..3928
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4028..4931
                     /label=PromGmUbi
     CDS             4952..5169
                     /codon_start=1
                     /label=GAL4 DNA binding domain (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=49.4"
                     /note="/other=CDS"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIF"
     CDS             5180..5413
                     /codon_start=1
                     /label=VP16 AD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             5420..6250
                     /codon_start=1
                     /label=GVG
                     /label=Nr3c1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=34.8"
                     /note="/other=CDS"
                     /translation="IQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLY
                     AGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLM
                     AFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEY
                     LCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLL
                     DSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
     terminator      6280..6576
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=46.3"
                     /note="/other=terminator"
     terminator      6705..6763
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=9.2"
                     /note="/other=terminator"
     protein_bind    6856..6873
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6874..6891
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6892..6910
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     protein_bind    6923..6940
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6941..6958
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6959..6977
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     promoter        7024..7070
                     /label=minimal CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    7114..7238
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        7273..7303
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        7327..7356
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             7357..8016
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             8195..8353
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             8358..8660
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(8704..8828)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             8857..8946
                     /codon_start=1
                     /label=3xHA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      8971..9229
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        9230..9756
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             9767..10477
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      10486..10738
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    10793..10817
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(10940..11528)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    11736..11919
                     /label=TetC term
     CDS             complement(join(11920..12230,1..880))
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
       61 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
      121 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
      181 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
      241 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
      301 agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
      361 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
      421 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
      481 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
      541 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc cacaggacgg
      601 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
      661 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
      721 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
      781 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      841 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      901 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
      961 gagaattctt gaaaatattg cgctgccatt tttggggtga ggccgttcgc ggccgagggg
     1021 cgcagcccct ggggggatgg gagctcggtg ccttgatgtg ggcgccggcg gtcgagtggc
     1081 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     1141 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     1201 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     1261 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     1321 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     1381 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     1441 acaggaaaga agctttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     1501 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     1561 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     1621 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     1681 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     1741 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     1801 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     1861 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     1921 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     1981 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     2041 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     2101 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     2161 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     2221 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     2281 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     2341 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacga cgcaccggct
     2401 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     2461 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     2521 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     2581 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     2641 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     2701 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     2761 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     2821 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     2881 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     2941 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     3001 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     3061 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     3121 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     3181 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     3241 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     3301 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     3361 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     3421 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     3481 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     3541 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     3601 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     3661 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     3721 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     3781 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggctgatgg
     3841 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3901 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     3961 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtgg aagacaatgc
     4021 ctccggagta acagataaag cgaagcttga aggtgcatgt gactccgtca agattacgaa
     4081 accgccaact accacgcaaa ttgcaattct caatttccta gaaggactct ccgaaaatgc
     4141 atccaatacc aaatattacc cgtgtcatag gcaccaagtg acaccataca tgaacacgcg
     4201 tcacaatatg actggagaag ggttccacac cttatgctat aaaacgcccc acacccctcc
     4261 tccttccttc gcagttcaat tccaatatat tccattctct ctgtgtattt ccctacctct
     4321 cccttcaagg ttagtcgatt tcttctgttt ttcttcttcg ttctttccat gaattgtgta
     4381 tgttctttga tcaatacgat gttgatttga ttgtgttttg tttggtttca tcgatcttca
     4441 attttcataa tcagattcag cttttattat ctttacaaca acgtccttaa tttgatgatt
     4501 ctttaatcgt agatttgctc taattagagc tttttcatgt cagatccctt tacaacaagc
     4561 cttaattgtt gattcattaa tcgtagatta gggctttttt cattgattac ttcagatccg
     4621 ttaaacgtaa ccatagatca gggctttttc atgaattact tcagatccgt taaacaacag
     4681 ccttattttt tatacttctg tggtttttca agaaattgtt cagatccgtt gacaaaaagc
     4741 cttattcgtt gattctatat cgtttttcga gagatattgc tcagatctgt tagcaactgc
     4801 cttgtttgtt gattctattg ccgtggatta gggttttttt tcacgagatt gcttcagatc
     4861 cgtacttaag attacgtaat ggattttgat tctgatttat ctgtgattgt tgactcgaca
     4921 gcggccgcac cggcgcgcca tggcagatcc aatgaagcta ctgtcttcta tcgaacaagc
     4981 atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa
     5041 gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaagat ctccgctgac
     5101 tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact
     5161 gatttttcca aggtcgagcg cccccccgac cgatgtcagc ctgggggacg agctccactt
     5221 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     5281 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     5341 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     5401 cgagtacggt ggggatccaa ttcagcaagc cactgcagga gtctcacaag acacttcgga
     5461 aaatcctaac aaaacaatag ttcctgctgc attaccacag ctcaccccta ccttggtgtc
     5521 actgctggag gtgattgaac ccgaggtgtt gtatgcagga tatgatagct ctgttccaga
     5581 ttcagcatgg agaattatga ccacactcaa catgttaggt gggcgtcaag tgattgcagc
     5641 agtgaaatgg gcaaaggcga taccaggctt cagaaactta cacctggatg accaaatgac
     5701 cctgctacag tactcatgga tgtttctcat ggcatttgcc ctgggttgga gatcatacag
     5761 acaatcaagt ggaaacctgc tctgctttgc tcctgatctg attattaatg agcagagaat
     5821 gtctctaccc tgcatgtatg accaatgtaa acacatgctg tttgtctcct ctgaattaca
     5881 aagattgcag gtatcctatg aagagtatct ctgtatgaaa accttactgc ttctctcctc
     5941 agttcctaag gaaggtctga agagccaaga gttatttgat gagattcgaa tgacttatat
     6001 caaagagcta ggaaaagcca tcgtcaaaag ggaagggaac tccagtcaga actggcaacg
     6061 gttttaccaa ctgacaaagc ttctggactc catgcatgag gtggttgaga atctccttac
     6121 ctactgcttc cagacatttt tggataagac catgagtatt gaattcccag agatgttagc
     6181 tgaaatcatc actaatcaga taccaaaata ttcaaatgga aatatcaaaa agcttctgtt
     6241 tcatcaaaaa tgactcgacc taactgagta agctagcttg ttcgagtatt atggcattgg
     6301 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     6361 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     6421 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     6481 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc
     6541 tctaatgacc gaagttaata tgaggagtaa aacactagat ccccaaacaa gcttgaaact
     6601 gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac gttatgaccc
     6661 ccgccgatga cgcgggacaa gccgttttac gtttggaact gacatatgaa aatatttttt
     6721 aatgcatttt atgacttgcc aattgattga caacatgcat caatccgcgg ttatgactct
     6781 cttaagagag tcatgaattc gagcgggaat tgatcccccc tcgacagctt gcatgccggt
     6841 cgactctaga ggatccgggt gacagccctc cgacgggtga cagccctccg acgggtgaca
     6901 gccctccgaa ttctagagga tccgggtgac agccctccga cgggtgacag ccctccgacg
     6961 ggtgacagcc ctccgaattc gagctcagta cccggggatc tgtcgacctc gatcgagatc
     7021 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaag
     7081 ctagtcgact ctagcctcga atccttaggt accacaagtt tgtacaaaaa agctgaacga
     7141 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     7201 acataatact gtaaaacaca acatatccag tcactatggc ggccctcgag ggccgcatta
     7261 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     7321 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     7381 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     7441 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     7501 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     7561 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     7621 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     7681 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     7741 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     7801 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     7861 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     7921 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     7981 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     8041 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     8101 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     8161 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     8221 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     8281 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     8341 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     8401 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     8461 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     8521 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     8581 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     8641 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     8701 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     8761 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     8821 aaagtggttg ataacagcgg gttaattaac atcttttacc catacgatgt tcctgactat
     8881 gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat
     8941 tacgctgctc agtgcagcgc ttagcctagg ttcggttcga gtattatggc attgggaaaa
     9001 ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg
     9061 ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg
     9121 ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa
     9181 ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatca aggttccaga
     9241 aggtaattat ccaagatgta gcatcaagaa tccaatgttt acgggaaaaa ctatggaagt
     9301 attatgtgag ctcagcaaga agcagatcaa tatgcggcac atatgcaacc tatgttcaaa
     9361 aatgaagaat gtacagatac aagatcctat actgccagaa tacgaagaag aatacgtaga
     9421 aattgaaaaa gaagaaccag gcgaagaaaa gaatcttgaa gacgtaagca ctgacgacaa
     9481 caatgaaaag aagaagataa ggtcggtgat tgtgaaagag acatagagga cacatgtaag
     9541 gtggaaaatg taagggcgga aagtaacctt atcacaaagg aatcttatcc cccactactt
     9601 atccttttat atttttccgt gtcatttttg cccttgagtt ttcctatata aggaaccaag
     9661 ttcggcattt gtgaaaacaa gaaaaaattt ggtgtaagct attttctttg aagtactgag
     9721 gatacaactt cagagaaatt tgtaagtttg tgatccgcaa acaaaaatgg tgagcaaggg
     9781 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     9841 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     9901 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     9961 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
    10021 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
    10081 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
    10141 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
    10201 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
    10261 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
    10321 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
    10381 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
    10441 ccactccacc ggcggcatgg acgagctgta caagtgagat gtgcagatcg ttcaaacatt
    10501 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
    10561 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
    10621 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
    10681 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatccg
    10741 atgataagct gtcaaacatg agggacacga agtggtttaa actatcagtg tttgacagga
    10801 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
    10861 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc caaccacagg aaccgtaaaa
    10921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11341 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    11401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
    11701 aaaacaattc atccagtaaa atataatcga atattatgcg cacccgtggc caggacccaa
    11761 cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
    11821 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
    11881 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
    11941 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
    12001 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
    12061 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
    12121 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
    12181 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
//
