LOCUS       Exported               16047 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..16047
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(879..1508)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    1772..1796
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        1896..2799
                     /label=PromGmUbi
     CDS             2820..3037
                     /codon_start=1
                     /label=GAL4 DNA binding domain (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=49.4"
                     /note="/other=CDS"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIF"
     CDS             3048..3281
                     /codon_start=1
                     /label=VP16 AD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3288..4118
                     /codon_start=1
                     /label=GVG
                     /label=Nr3c1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=34.8"
                     /note="/other=CDS"
                     /translation="IQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLY
                     AGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLM
                     AFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEY
                     LCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLL
                     DSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
     terminator      4148..4444
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=46.3"
                     /note="/other=terminator"
     terminator      4573..4631
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=9.2"
                     /note="/other=terminator"
     protein_bind    4724..4741
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    4742..4759
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    4760..4778
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     protein_bind    4791..4808
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    4809..4826
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    4827..4845
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     promoter        4892..4938
                     /label=minimal CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4982..5698
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    5705..5829
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        5854..5884
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        5908..5937
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             5938..6597
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6776..6934
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             6939..7241
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(7285..7409)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     terminator      7416..7674
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        7675..8201
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             8212..12162
                     /label=RUBY
     terminator      12171..12423
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    12478..12502
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(12625..13213)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    13421..13604
                     /label=TetC term
     CDS             complement(13605..14795)
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    complement(14796..14888)
                     /label=PromTet
     misc_feature    15164..15358
                     /label=pVS1 oriV
     CDS             complement(join(15424..16047,1..450))
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
       61 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
      121 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
      181 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
      241 cggcttccta atcgacgacg caccggctgc cggcggttgc cgggattctt tgcggattcg
      301 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
      361 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
      421 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
      481 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
      541 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
      601 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
      661 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
      721 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
      781 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
      841 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
      901 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
      961 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     1021 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     1081 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     1141 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     1201 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     1261 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     1321 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
     1381 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
     1441 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
     1501 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
     1561 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
     1621 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
     1681 ggatttcatg cagccgcacg gctgatgggc tgcctgtatc gagtggtgat tttgtgccga
     1741 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     1801 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca
     1861 gtgggcccca ctctgtggaa gacaatgcct ccggagtaac agataaagcg aagcttgaag
     1921 gtgcatgtga ctccgtcaag attacgaaac cgccaactac cacgcaaatt gcaattctca
     1981 atttcctaga aggactctcc gaaaatgcat ccaataccaa atattacccg tgtcataggc
     2041 accaagtgac accatacatg aacacgcgtc acaatatgac tggagaaggg ttccacacct
     2101 tatgctataa aacgccccac acccctcctc cttccttcgc agttcaattc caatatattc
     2161 cattctctct gtgtatttcc ctacctctcc cttcaaggtt agtcgatttc ttctgttttt
     2221 cttcttcgtt ctttccatga attgtgtatg ttctttgatc aatacgatgt tgatttgatt
     2281 gtgttttgtt tggtttcatc gatcttcaat tttcataatc agattcagct tttattatct
     2341 ttacaacaac gtccttaatt tgatgattct ttaatcgtag atttgctcta attagagctt
     2401 tttcatgtca gatcccttta caacaagcct taattgttga ttcattaatc gtagattagg
     2461 gcttttttca ttgattactt cagatccgtt aaacgtaacc atagatcagg gctttttcat
     2521 gaattacttc agatccgtta aacaacagcc ttatttttta tacttctgtg gtttttcaag
     2581 aaattgttca gatccgttga caaaaagcct tattcgttga ttctatatcg tttttcgaga
     2641 gatattgctc agatctgtta gcaactgcct tgtttgttga ttctattgcc gtggattagg
     2701 gttttttttc acgagattgc ttcagatccg tacttaagat tacgtaatgg attttgattc
     2761 tgatttatct gtgattgttg actcgacagc ggccgcaccg gcgcgccatg gcagatccaa
     2821 tgaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt
     2881 gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact
     2941 ctcccaaaac caaaagatct ccgctgacta gggcacatct gacagaagtg gaatcaaggc
     3001 tagaaagact ggaacagcta tttctactga tttttccaag gtcgagcgcc cccccgaccg
     3061 atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg gcgcatgccg
     3121 acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg ggtccgggat
     3181 ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc gagtttgagc
     3241 agatgtttac cgatgccctt ggaattgacg agtacggtgg ggatccaatt cagcaagcca
     3301 ctgcaggagt ctcacaagac acttcggaaa atcctaacaa aacaatagtt cctgctgcat
     3361 taccacagct cacccctacc ttggtgtcac tgctggaggt gattgaaccc gaggtgttgt
     3421 atgcaggata tgatagctct gttccagatt cagcatggag aattatgacc acactcaaca
     3481 tgttaggtgg gcgtcaagtg attgcagcag tgaaatgggc aaaggcgata ccaggcttca
     3541 gaaacttaca cctggatgac caaatgaccc tgctacagta ctcatggatg tttctcatgg
     3601 catttgccct gggttggaga tcatacagac aatcaagtgg aaacctgctc tgctttgctc
     3661 ctgatctgat tattaatgag cagagaatgt ctctaccctg catgtatgac caatgtaaac
     3721 acatgctgtt tgtctcctct gaattacaaa gattgcaggt atcctatgaa gagtatctct
     3781 gtatgaaaac cttactgctt ctctcctcag ttcctaagga aggtctgaag agccaagagt
     3841 tatttgatga gattcgaatg acttatatca aagagctagg aaaagccatc gtcaaaaggg
     3901 aagggaactc cagtcagaac tggcaacggt tttaccaact gacaaagctt ctggactcca
     3961 tgcatgaggt ggttgagaat ctccttacct actgcttcca gacatttttg gataagacca
     4021 tgagtattga attcccagag atgttagctg aaatcatcac taatcagata ccaaaatatt
     4081 caaatggaaa tatcaaaaag cttctgtttc atcaaaaatg actcgaccta actgagtaag
     4141 ctagcttgtt cgagtattat ggcattggga aaactgtttt tcttgtacca tttgttgtgc
     4201 ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat ggaaatggat
     4261 ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat attatttgtt
     4321 ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa acattttgtt
     4381 ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg aggagtaaaa
     4441 cactagatcc ccaaacaagc ttgaaactga aggcgggaaa cgacaatctg atcatgagcg
     4501 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
     4561 ttggaactga catatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca
     4621 acatgcatca atccgcggtt atgactctct taagagagtc atgaattcga gcgggaattg
     4681 atcccccctc gacagcttgc atgccggtcg actctagagg atccgggtga cagccctccg
     4741 acgggtgaca gccctccgac gggtgacagc cctccgaatt ctagaggatc cgggtgacag
     4801 ccctccgacg ggtgacagcc ctccgacggg tgacagccct ccgaattcga gctcagtacc
     4861 cggggatctg tcgacctcga tcgagatctt cgcaagaccc ttcctctata taaggaagtt
     4921 catttcattt ggagaggaca cgctgaagct agtcgactct agcctcgaat ccttaggtac
     4981 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5041 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5101 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5161 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5221 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5281 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5341 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5401 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5461 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5521 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5581 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5641 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagga
     5701 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     5761 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     5821 gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5881 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
     5941 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     6001 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     6061 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     6121 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     6181 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     6241 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     6301 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     6361 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     6421 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     6481 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     6541 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg
     6601 cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     6661 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     6721 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     6781 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     6841 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     6901 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     6961 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     7021 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     7081 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     7141 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     7201 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     7261 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     7321 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     7381 tctcgttcag ctttcttgta caaagtggtc ctaggttcgg ttcgagtatt atggcattgg
     7441 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     7501 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     7561 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     7621 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcaaggtt
     7681 ccagaaggta attatccaag atgtagcatc aagaatccaa tgtttacggg aaaaactatg
     7741 gaagtattat gtgagctcag caagaagcag atcaatatgc ggcacatatg caacctatgt
     7801 tcaaaaatga agaatgtaca gatacaagat cctatactgc cagaatacga agaagaatac
     7861 gtagaaattg aaaaagaaga accaggcgaa gaaaagaatc ttgaagacgt aagcactgac
     7921 gacaacaatg aaaagaagaa gataaggtcg gtgattgtga aagagacata gaggacacat
     7981 gtaaggtgga aaatgtaagg gcggaaagta accttatcac aaaggaatct tatcccccac
     8041 tacttatcct tttatatttt tccgtgtcat ttttgccctt gagttttcct atataaggaa
     8101 ccaagttcgg catttgtgaa aacaagaaaa aatttggtgt aagctatttt ctttgaagta
     8161 ctgaggatac aacttcagag aaatttgtaa gtttgtgatc cgcaaacaaa aatggatcat
     8221 gcgaccctcg ccatgatcct cgcgatctgg ttcatcagct tccacttcat caagctgctg
     8281 ttctcccagc agaccaccaa gctgcttccg ccaggaccaa agccgcttcc gatcatcggc
     8341 aacatccttg aggtgggcaa gaagccgcat cggtccttcg ccaacctcgc caagattcac
     8401 ggcccactca tttccctcag actcggctct gtgaccacca tcgttgtgtc ctctgccgac
     8461 gtggccaaag agatgttcct caagaaggat cacccgctct ccaaccgcac gatcccgaat
     8521 agtgttacag ccggcgacca ccacaagctc accatgtctt ggctcccggt gtctccgaag
     8581 tggcgcaact tccgcaagat taccgccgtg catctgctct ccccacagag actcgatgcc
     8641 tgccagacat tcaggcacgc caaggtgcag cagctctacg agtacgttca agagtgcgcc
     8701 cagaaaggcc aggccgtgga tattggcaag gccgccttta cgaccagcct caacctcctc
     8761 agcaagctgt tcttcagcgt cgagctggcg caccacaagt cccataccag ccaagagttc
     8821 aaagagctga tctggaacat catggaagat ataggcaagc cgaactacgc cgactacttc
     8881 ccgattctcg gctgcgttga cccatctggc attagaagaa ggctcgcctg ctccttcgac
     8941 aagctgatcg ccgtgttcca gggcatcatc tgcgagagac tcgccccaga ttcctccacc
     9001 acaactacca ccaccaccga cgacgtgctc gatgtgctcc tccagctgtt caagcagaac
     9061 gagctgacga tgggcgagat caaccacctc ctcgtggaca tcttcgacgc cggcaccgat
     9121 accacatcct ccacattcga gtgggtgatg accgagctga tccgcaatcc agagatgatg
     9181 gaaaaggccc aagaggaaat caagcaggtc ctcggcaagg acaagcagat ccaagagtcc
     9241 gacatcatca acctgccgta cctccaggcg atcatcaaag agacactccg cctccatccg
     9301 ccgaccgtgt tcttgctccc aagaaaggcc gacaccgatg tcgagctgta cggctacatc
     9361 gtgccgaagg atgcccagat cctcgtgaac ctctgggcca ttggcaggga cccaaacgcc
     9421 tggcagaacg ccgatatttt cagcccagag cgcttcatcg gctgcgagat cgatgttaag
     9481 ggccgcgatt tcggcctcct tccatttggc gctggccgca gaatttgccc aggcatgaat
     9541 ctcgccatca ggatgctcac cctcatgctc gccacactcc tccagttctt caactggaag
     9601 ctcgaaggcg acatctcccc gaaggacctc gacatggacg agaagttcgg cattgcgctc
     9661 caaaagacca agccgctcaa gctcatcccg attccgcgct acggtagcgg agctaccaat
     9721 tttagcctcc ttaagcaggc aggtgatgta gaagagaacc ccgggcctat gaagatgatg
     9781 aacggcgagg acgccaacga ccagatgatc aaagagtcct tcttcatcac ccacggcaac
     9841 ccgatcctca ccgtcgagga tacacatccg ctcaggccgt tcttcgagac atggcgcgag
     9901 aagattttct ccaagaagcc gaaggccatc ctcatcatct ccggccactg ggagacagtg
     9961 aagccaaccg tgaacgccgt gcacatcaac gacaccatcc acgacttcga cgactaccca
    10021 gccgccatgt accagttcaa gtacccagct ccaggcgagc cagagcttgc gagaaaggtg
    10081 gaagagatcc tcaagaagtc cgggttcgag acagccgaga cagaccaaaa gaggggcctt
    10141 gatcacggcg cctgggttcc actcatgctc atgtatccag aggcggacat cccggtgtgc
    10201 cagctctcag ttcagccaca tctcgacggc acctaccact acaatctcgg cagagccctc
    10261 gcgccgctca agaatgatgg cgtgctcatt attggctccg gcagcgccac acatccactc
    10321 gatgagacac cgcactactt cgatggtgtt gccccttggg ccgctgcctt cgattcttgg
    10381 cttaggaagg ccctcatcaa cggccgcttc gaggaagtga acatctacga gagcaaggcc
    10441 ccgaactgga agctcgccca tccatttcca gagcacttct acccgctcca cgttgtgctc
    10501 ggcgctgctg gtgaaaagtg gaaggccgag ctgatccact cctcctggga tcatggcaca
    10561 ctttgccacg gctcctacaa gttcacctcc gccggatccg gagcaaccaa ctttagcctg
    10621 ctcaagcaag caggagatgt tgaggaaaat cctggcccca tgaccgccat caagatgaac
    10681 accaacggcg agggcgagac acagcacatc ctcatgatcc cgttcatggc gcagggccac
    10741 ctcaggccat ttctcgaact cgccatgttc ctctacaagc gctcccacgt gatcatcacc
    10801 ctgctcacaa ctccgctcaa tgccggcttc ctcaggcacc tccttcacca ccattcctac
    10861 tcctccagcg gcatcaggat cgtcgagctg ccattcaact ccaccaacca cggactccca
    10921 ccgggcatcg agaacaccga taagctcaca ctcccgctcg tggtgtccct cttccattcc
    10981 accatcagcc tcgatccgca cctccgcgat tacatctcca ggcatttcag cccagccagg
    11041 ccaccactct gcgtgatcca tgatgtgttc ctcggctggg ttgaccaggt ggccaaggat
    11101 gtgggctcta caggcgtggt gttcacaaca ggcggcgctt atggcacatc cgcctacgtg
    11161 tccatctgga acgatctccc gcaccagaac tactccgacg accaagagtt cccgctgcca
    11221 ggcttcccag agaaccataa gttccgcagg tcccagctcc atcggttcct cagatatgcc
    11281 gacggctccg acgattggtc caagtatttc cagccgcagc tccgccagtc catgaagtct
    11341 tttggctggc tctgcaactc cgtggaagag atcgagacac tcggcttctc catcctccgc
    11401 aactacacca agctgccgat ctggggcatc ggcccactta ttgcttcccc agtgcagcac
    11461 tcctcctccg acaacaattc aacaggcgcc gagttcgtgc agtggctcag cctcaaagag
    11521 ccggactccg tcctctacat ctccttcggc tcccagaaca cgatcagccc gacgcagatg
    11581 atggaactcg ctgctggcct tgagtcctcc gagaagccat tcctctgggt gatcagagcc
    11641 ccgttcggct tcgacatcaa cgaagagatg cgcccagagt ggctgccaga gggctttgag
    11701 gaacgcatga aggtgaagaa acagggcaag ctcgtgtaca agctcggccc gcagcttgag
    11761 atcctcaacc atgaatccat cggcggcttt ctcacccact gcggatggaa cagcatcctt
    11821 gagtctcttc gcgagggcgt tccgatgctt ggatggccac ttgctgccga gcaggcctac
    11881 aacctcaagt acctcgaaga tgagatgggc gtcgcggttg agcttgctag aggcctcgaa
    11941 ggcgagatct ccaaagagaa ggtcaagcgc atcgtcgaga tgatccttga gcgcaacgag
    12001 ggctccaaag gctgggagat gaagaatcgc gccgtggaaa tgggcaaaaa gctcaaggac
    12061 gccgtgaacg aggaaaaaga gctgaagggc tcctccgtga aggcgatcga cgatttcctc
    12121 gacgccgtca tgcaggccaa acttgagcca agcctccagt gagatgtgca gatcgttcaa
    12181 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
    12241 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
    12301 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
    12361 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
    12421 atccgatgat aagctgtcaa acatgaggga cacgaagtgg tttaaactat cagtgtttga
    12481 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    12541 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgccaacc acaggaaccg
    12601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    12661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    12721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    12781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    12841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    12901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    12961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    13021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    13081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    13141 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    13201 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    13261 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    13321 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    13381 cagctaaaac aattcatcca gtaaaatata atcgaatatt atgcgcaccc gtggccagga
    13441 cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
    13501 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
    13561 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
    13621 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
    13681 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
    13741 ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
    13801 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
    13861 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
    13921 acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
    13981 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca
    14041 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
    14101 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
    14161 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
    14221 atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
    14281 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
    14341 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
    14401 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
    14461 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg
    14521 gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg
    14581 cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca
    14641 tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc
    14701 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
    14761 ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa
    14821 tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
    14881 ttcttgaaaa tattgcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag
    14941 cccctggggg gatgggagct cggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
    15001 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
    15061 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
    15121 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
    15181 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
    15241 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
    15301 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
    15361 aaagaagctt tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
    15421 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
    15481 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
    15541 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
    15601 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
    15661 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
    15721 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
    15781 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
    15841 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
    15901 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
    15961 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
    16021 cttcccttcc cggtatcggt tcatgga
//
