LOCUS       Exported               15471 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             555..713
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             718..1020
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1064..1188)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     terminator      1195..1453
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        1454..1980
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             1991..5941
                     /codon_start=1
                     /label=RUBY
                     /translation="MDHATLAMILAIWFISFHFIKLLFSQQTTKLLPPGPKPLPIIGNI
                     LEVGKKPHRSFANLAKIHGPLISLRLGSVTTIVVSSADVAKEMFLKKDHPLSNRTIPNS
                     VTAGDHHKLTMSWLPVSPKWRNFRKITAVHLLSPQRLDACQTFRHAKVQQLYEYVQECA
                     QKGQAVDIGKAAFTTSLNLLSKLFFSVELAHHKSHTSQEFKELIWNIMEDIGKPNYADY
                     FPILGCVDPSGIRRRLACSFDKLIAVFQGIICERLAPDSSTTTTTTTDDVLDVLLQLFK
                     QNELTMGEINHLLVDIFDAGTDTTSSTFEWVMTELIRNPEMMEKAQEEIKQVLGKDKQI
                     QESDIINLPYLQAIIKETLRLHPPTVFLLPRKADTDVELYGYIVPKDAQILVNLWAIGR
                     DPNAWQNADIFSPERFIGCEIDVKGRDFGLLPFGAGRRICPGMNLAIRMLTLMLATLLQ
                     FFNWKLEGDISPKDLDMDEKFGIALQKTKPLKLIPIPRYGSGATNFSLLKQAGDVEENP
                     GPMKMMNGEDANDQMIKESFFITHGNPILTVEDTHPLRPFFETWREKIFSKKPKAILII
                     SGHWETVKPTVNAVHINDTIHDFDDYPAAMYQFKYPAPGEPELARKVEEILKKSGFETA
                     ETDQKRGLDHGAWVPLMLMYPEADIPVCQLSVQPHLDGTYHYNLGRALAPLKNDGVLII
                     GSGSATHPLDETPHYFDGVAPWAAAFDSWLRKALINGRFEEVNIYESKAPNWKLAHPFP
                     EHFYPLHVVLGAAGEKWKAELIHSSWDHGTLCHGSYKFTSAGSGATNFSLLKQAGDVEE
                     NPGPMTAIKMNTNGEGETQHILMIPFMAQGHLRPFLELAMFLYKRSHVIITLLTTPLNA
                     GFLRHLLHHHSYSSSGIRIVELPFNSTNHGLPPGIENTDKLTLPLVVSLFHSTISLDPH
                     LRDYISRHFSPARPPLCVIHDVFLGWVDQVAKDVGSTGVVFTTGGAYGTSAYVSIWNDL
                     PHQNYSDDQEFPLPGFPENHKFRRSQLHRFLRYADGSDDWSKYFQPQLRQSMKSFGWLC
                     NSVEEIETLGFSILRNYTKLPIWGIGPLIASPVQHSSSDNNSTGAEFVQWLSLKEPDSV
                     LYISFGSQNTISPTQMMELAAGLESSEKPFLWVIRAPFGFDINEEMRPEWLPEGFEERM
                     KVKKQGKLVYKLGPQLEILNHESIGGFLTHCGWNSILESLREGVPMLGWPLAAEQAYNL
                     KYLEDEMGVAVELARGLEGEISKEKVKRIVEMILERNEGSKGWEMKNRAVEMGKKLKDA
                     VNEEKELKGSSVKAIDDFLDAVMQAKLEPSLQ"
     terminator      5950..6202
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    6257..6281
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(6404..6992)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    7200..7383
                     /label=TetC term
     CDS             complement(7384..8574)
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    complement(8575..8667)
                     /label=PromTet
     rep_origin      complement(8943..9137)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(9203..10276)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10705..11334)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11598..11622
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        11722..12625
                     /label=PromGmUbi
     CDS             12646..12863
                     /codon_start=1
                     /label=GAL4 DNA binding domain (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=49.4"
                     /note="/other=CDS"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIF"
     CDS             12874..13107
                     /codon_start=1
                     /label=VP16 AD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             13114..13944
                     /codon_start=1
                     /label=GVG
                     /label=Nr3c1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=34.8"
                     /note="/other=CDS"
                     /translation="IQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLY
                     AGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLM
                     AFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEY
                     LCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLL
                     DSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
     terminator      13974..14270
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=46.3"
                     /note="/other=terminator"
     terminator      14399..14457
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=9.2"
                     /note="/other=terminator"
     protein_bind    14550..14567
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14568..14585
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14586..14604
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     protein_bind    14617..14634
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14635..14652
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14653..14671
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     promoter        14718..14764
                     /label=minimal CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             14832..14921
                     /codon_start=1
                     /label=3xHA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    14943..15067
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        15104..15134
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        15158..15187
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             join(15188..15471,1..376)
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
       61 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      121 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
      181 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
      241 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
      301 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
      361 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
      421 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
      481 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
      541 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
      601 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
      661 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
      721 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
      781 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
      841 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
      901 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
      961 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     1021 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     1081 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     1141 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtcc taggttcggt
     1201 tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
     1261 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
     1321 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
     1381 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
     1441 aatgtgtcaa atcaaggttc cagaaggtaa ttatccaaga tgtagcatca agaatccaat
     1501 gtttacggga aaaactatgg aagtattatg tgagctcagc aagaagcaga tcaatatgcg
     1561 gcacatatgc aacctatgtt caaaaatgaa gaatgtacag atacaagatc ctatactgcc
     1621 agaatacgaa gaagaatacg tagaaattga aaaagaagaa ccaggcgaag aaaagaatct
     1681 tgaagacgta agcactgacg acaacaatga aaagaagaag ataaggtcgg tgattgtgaa
     1741 agagacatag aggacacatg taaggtggaa aatgtaaggg cggaaagtaa ccttatcaca
     1801 aaggaatctt atcccccact acttatcctt ttatattttt ccgtgtcatt tttgcccttg
     1861 agttttccta tataaggaac caagttcggc atttgtgaaa acaagaaaaa atttggtgta
     1921 agctattttc tttgaagtac tgaggataca acttcagaga aatttgtaag tttgtgatcc
     1981 gcaaacaaaa atggatcatg cgaccctcgc catgatcctc gcgatctggt tcatcagctt
     2041 ccacttcatc aagctgctgt tctcccagca gaccaccaag ctgcttccgc caggaccaaa
     2101 gccgcttccg atcatcggca acatccttga ggtgggcaag aagccgcatc ggtccttcgc
     2161 caacctcgcc aagattcacg gcccactcat ttccctcaga ctcggctctg tgaccaccat
     2221 cgttgtgtcc tctgccgacg tggccaaaga gatgttcctc aagaaggatc acccgctctc
     2281 caaccgcacg atcccgaata gtgttacagc cggcgaccac cacaagctca ccatgtcttg
     2341 gctcccggtg tctccgaagt ggcgcaactt ccgcaagatt accgccgtgc atctgctctc
     2401 cccacagaga ctcgatgcct gccagacatt caggcacgcc aaggtgcagc agctctacga
     2461 gtacgttcaa gagtgcgccc agaaaggcca ggccgtggat attggcaagg ccgcctttac
     2521 gaccagcctc aacctcctca gcaagctgtt cttcagcgtc gagctggcgc accacaagtc
     2581 ccataccagc caagagttca aagagctgat ctggaacatc atggaagata taggcaagcc
     2641 gaactacgcc gactacttcc cgattctcgg ctgcgttgac ccatctggca ttagaagaag
     2701 gctcgcctgc tccttcgaca agctgatcgc cgtgttccag ggcatcatct gcgagagact
     2761 cgccccagat tcctccacca caactaccac caccaccgac gacgtgctcg atgtgctcct
     2821 ccagctgttc aagcagaacg agctgacgat gggcgagatc aaccacctcc tcgtggacat
     2881 cttcgacgcc ggcaccgata ccacatcctc cacattcgag tgggtgatga ccgagctgat
     2941 ccgcaatcca gagatgatgg aaaaggccca agaggaaatc aagcaggtcc tcggcaagga
     3001 caagcagatc caagagtccg acatcatcaa cctgccgtac ctccaggcga tcatcaaaga
     3061 gacactccgc ctccatccgc cgaccgtgtt cttgctccca agaaaggccg acaccgatgt
     3121 cgagctgtac ggctacatcg tgccgaagga tgcccagatc ctcgtgaacc tctgggccat
     3181 tggcagggac ccaaacgcct ggcagaacgc cgatattttc agcccagagc gcttcatcgg
     3241 ctgcgagatc gatgttaagg gccgcgattt cggcctcctt ccatttggcg ctggccgcag
     3301 aatttgccca ggcatgaatc tcgccatcag gatgctcacc ctcatgctcg ccacactcct
     3361 ccagttcttc aactggaagc tcgaaggcga catctccccg aaggacctcg acatggacga
     3421 gaagttcggc attgcgctcc aaaagaccaa gccgctcaag ctcatcccga ttccgcgcta
     3481 cggtagcgga gctaccaatt ttagcctcct taagcaggca ggtgatgtag aagagaaccc
     3541 cgggcctatg aagatgatga acggcgagga cgccaacgac cagatgatca aagagtcctt
     3601 cttcatcacc cacggcaacc cgatcctcac cgtcgaggat acacatccgc tcaggccgtt
     3661 cttcgagaca tggcgcgaga agattttctc caagaagccg aaggccatcc tcatcatctc
     3721 cggccactgg gagacagtga agccaaccgt gaacgccgtg cacatcaacg acaccatcca
     3781 cgacttcgac gactacccag ccgccatgta ccagttcaag tacccagctc caggcgagcc
     3841 agagcttgcg agaaaggtgg aagagatcct caagaagtcc gggttcgaga cagccgagac
     3901 agaccaaaag aggggccttg atcacggcgc ctgggttcca ctcatgctca tgtatccaga
     3961 ggcggacatc ccggtgtgcc agctctcagt tcagccacat ctcgacggca cctaccacta
     4021 caatctcggc agagccctcg cgccgctcaa gaatgatggc gtgctcatta ttggctccgg
     4081 cagcgccaca catccactcg atgagacacc gcactacttc gatggtgttg ccccttgggc
     4141 cgctgccttc gattcttggc ttaggaaggc cctcatcaac ggccgcttcg aggaagtgaa
     4201 catctacgag agcaaggccc cgaactggaa gctcgcccat ccatttccag agcacttcta
     4261 cccgctccac gttgtgctcg gcgctgctgg tgaaaagtgg aaggccgagc tgatccactc
     4321 ctcctgggat catggcacac tttgccacgg ctcctacaag ttcacctccg ccggatccgg
     4381 agcaaccaac tttagcctgc tcaagcaagc aggagatgtt gaggaaaatc ctggccccat
     4441 gaccgccatc aagatgaaca ccaacggcga gggcgagaca cagcacatcc tcatgatccc
     4501 gttcatggcg cagggccacc tcaggccatt tctcgaactc gccatgttcc tctacaagcg
     4561 ctcccacgtg atcatcaccc tgctcacaac tccgctcaat gccggcttcc tcaggcacct
     4621 ccttcaccac cattcctact cctccagcgg catcaggatc gtcgagctgc cattcaactc
     4681 caccaaccac ggactcccac cgggcatcga gaacaccgat aagctcacac tcccgctcgt
     4741 ggtgtccctc ttccattcca ccatcagcct cgatccgcac ctccgcgatt acatctccag
     4801 gcatttcagc ccagccaggc caccactctg cgtgatccat gatgtgttcc tcggctgggt
     4861 tgaccaggtg gccaaggatg tgggctctac aggcgtggtg ttcacaacag gcggcgctta
     4921 tggcacatcc gcctacgtgt ccatctggaa cgatctcccg caccagaact actccgacga
     4981 ccaagagttc ccgctgccag gcttcccaga gaaccataag ttccgcaggt cccagctcca
     5041 tcggttcctc agatatgccg acggctccga cgattggtcc aagtatttcc agccgcagct
     5101 ccgccagtcc atgaagtctt ttggctggct ctgcaactcc gtggaagaga tcgagacact
     5161 cggcttctcc atcctccgca actacaccaa gctgccgatc tggggcatcg gcccacttat
     5221 tgcttcccca gtgcagcact cctcctccga caacaattca acaggcgccg agttcgtgca
     5281 gtggctcagc ctcaaagagc cggactccgt cctctacatc tccttcggct cccagaacac
     5341 gatcagcccg acgcagatga tggaactcgc tgctggcctt gagtcctccg agaagccatt
     5401 cctctgggtg atcagagccc cgttcggctt cgacatcaac gaagagatgc gcccagagtg
     5461 gctgccagag ggctttgagg aacgcatgaa ggtgaagaaa cagggcaagc tcgtgtacaa
     5521 gctcggcccg cagcttgaga tcctcaacca tgaatccatc ggcggctttc tcacccactg
     5581 cggatggaac agcatccttg agtctcttcg cgagggcgtt ccgatgcttg gatggccact
     5641 tgctgccgag caggcctaca acctcaagta cctcgaagat gagatgggcg tcgcggttga
     5701 gcttgctaga ggcctcgaag gcgagatctc caaagagaag gtcaagcgca tcgtcgagat
     5761 gatccttgag cgcaacgagg gctccaaagg ctgggagatg aagaatcgcg ccgtggaaat
     5821 gggcaaaaag ctcaaggacg ccgtgaacga ggaaaaagag ctgaagggct cctccgtgaa
     5881 ggcgatcgac gatttcctcg acgccgtcat gcaggccaaa cttgagccaa gcctccagtg
     5941 agatgtgcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     6001 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     6061 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     6121 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     6181 gtgtcatcta tgttactaga tccgatgata agctgtcaaa catgagggac acgaagtggt
     6241 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     6301 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     6361 gtgccaacca caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6841 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7141 atgagtaaac ttggtctgac agctaaaaca attcatccag taaaatataa tcgaatatta
     7201 tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga
     7261 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     7321 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
     7381 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     7441 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
     7501 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
     7561 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     7621 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     7681 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
     7741 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     7801 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     7861 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     7921 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     7981 gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa
     8041 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     8101 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     8161 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     8221 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     8281 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     8341 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     8401 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     8461 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     8521 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     8581 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat
     8641 aagctgtcaa acatgagaat tcttgaaaat attgcgctgc catttttggg gtgaggccgt
     8701 tcgcggccga ggggcgcagc ccctgggggg atgggagctc ggtgccttga tgtgggcgcc
     8761 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     8821 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     8881 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     8941 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     9001 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     9061 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     9121 tgtacgtgct atccacagga aagaagcttt ttcgaccttt ttcccctgct agggcaattt
     9181 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     9241 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     9301 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     9361 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     9421 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     9481 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     9541 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     9601 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     9661 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     9721 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     9781 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     9841 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     9901 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     9961 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
    10021 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
    10081 acgacgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
    10141 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
    10201 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
    10261 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
    10321 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    10381 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    10441 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    10501 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    10561 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    10621 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    10681 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    10741 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    10801 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    10861 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    10921 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    10981 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    11041 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    11101 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    11161 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    11221 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    11281 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    11341 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    11401 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    11461 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    11521 cgcacggctg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    11581 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    11641 cttaataaca cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccactct
    11701 gtggaagaca atgcctccgg agtaacagat aaagcgaagc ttgaaggtgc atgtgactcc
    11761 gtcaagatta cgaaaccgcc aactaccacg caaattgcaa ttctcaattt cctagaagga
    11821 ctctccgaaa atgcatccaa taccaaatat tacccgtgtc ataggcacca agtgacacca
    11881 tacatgaaca cgcgtcacaa tatgactgga gaagggttcc acaccttatg ctataaaacg
    11941 ccccacaccc ctcctccttc cttcgcagtt caattccaat atattccatt ctctctgtgt
    12001 atttccctac ctctcccttc aaggttagtc gatttcttct gtttttcttc ttcgttcttt
    12061 ccatgaattg tgtatgttct ttgatcaata cgatgttgat ttgattgtgt tttgtttggt
    12121 ttcatcgatc ttcaattttc ataatcagat tcagctttta ttatctttac aacaacgtcc
    12181 ttaatttgat gattctttaa tcgtagattt gctctaatta gagctttttc atgtcagatc
    12241 cctttacaac aagccttaat tgttgattca ttaatcgtag attagggctt ttttcattga
    12301 ttacttcaga tccgttaaac gtaaccatag atcagggctt tttcatgaat tacttcagat
    12361 ccgttaaaca acagccttat tttttatact tctgtggttt ttcaagaaat tgttcagatc
    12421 cgttgacaaa aagccttatt cgttgattct atatcgtttt tcgagagata ttgctcagat
    12481 ctgttagcaa ctgccttgtt tgttgattct attgccgtgg attagggttt tttttcacga
    12541 gattgcttca gatccgtact taagattacg taatggattt tgattctgat ttatctgtga
    12601 ttgttgactc gacagcggcc gcaccggcgc gccatggcag atccaatgaa gctactgtct
    12661 tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa
    12721 ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa
    12781 agatctccgc tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa
    12841 cagctatttc tactgatttt tccaaggtcg agcgcccccc cgaccgatgt cagcctgggg
    12901 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
    12961 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
    13021 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
    13081 gcccttggaa ttgacgagta cggtggggat ccaattcagc aagccactgc aggagtctca
    13141 caagacactt cggaaaatcc taacaaaaca atagttcctg ctgcattacc acagctcacc
    13201 cctaccttgg tgtcactgct ggaggtgatt gaacccgagg tgttgtatgc aggatatgat
    13261 agctctgttc cagattcagc atggagaatt atgaccacac tcaacatgtt aggtgggcgt
    13321 caagtgattg cagcagtgaa atgggcaaag gcgataccag gcttcagaaa cttacacctg
    13381 gatgaccaaa tgaccctgct acagtactca tggatgtttc tcatggcatt tgccctgggt
    13441 tggagatcat acagacaatc aagtggaaac ctgctctgct ttgctcctga tctgattatt
    13501 aatgagcaga gaatgtctct accctgcatg tatgaccaat gtaaacacat gctgtttgtc
    13561 tcctctgaat tacaaagatt gcaggtatcc tatgaagagt atctctgtat gaaaacctta
    13621 ctgcttctct cctcagttcc taaggaaggt ctgaagagcc aagagttatt tgatgagatt
    13681 cgaatgactt atatcaaaga gctaggaaaa gccatcgtca aaagggaagg gaactccagt
    13741 cagaactggc aacggtttta ccaactgaca aagcttctgg actccatgca tgaggtggtt
    13801 gagaatctcc ttacctactg cttccagaca tttttggata agaccatgag tattgaattc
    13861 ccagagatgt tagctgaaat catcactaat cagataccaa aatattcaaa tggaaatatc
    13921 aaaaagcttc tgtttcatca aaaatgactc gacctaactg agtaagctag cttgttcgag
    13981 tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt
    14041 gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat
    14101 gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg
    14161 ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt
    14221 gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact agatccccaa
    14281 acaagcttga aactgaaggc gggaaacgac aatctgatca tgagcggaga attaagggag
    14341 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacata
    14401 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaatcc
    14461 gcggttatga ctctcttaag agagtcatga attcgagcgg gaattgatcc cccctcgaca
    14521 gcttgcatgc cggtcgactc tagaggatcc gggtgacagc cctccgacgg gtgacagccc
    14581 tccgacgggt gacagccctc cgaattctag aggatccggg tgacagccct ccgacgggtg
    14641 acagccctcc gacgggtgac agccctccga attcgagctc agtacccggg gatctgtcga
    14701 cctcgatcga gatcttcgca agacccttcc tctatataag gaagttcatt tcatttggag
    14761 aggacacgct gaagctagtc gactctagcc tcgaatcctt aggtaccatg agcgggttaa
    14821 ttaacatctt ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
    14881 actatgcagg atcctatcca tatgacgttc cagattacgc tgctcagtgc agcgctgtta
    14941 tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
    15001 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
    15061 cactatggcg gcccctcgag gggccgcatt aggcacccca ggctttacac tttatgcttc
    15121 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
    15181 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
    15241 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
    15301 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
    15361 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
    15421 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt t
//
