LOCUS       Exported               16203 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             546..704
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             709..1011
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1055..1179)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     terminator      1189..1447
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        1448..1974
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             1985..5935
                     /label=RUBY
     terminator      5944..6196
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    6251..6275
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(6398..6986)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    7194..7377
                     /label=TetC term
     CDS             complement(7378..8568)
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    complement(8569..8661)
                     /label=PromTet
     rep_origin      complement(8936..9130)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(9196..10269)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10698..11327)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11591..11615
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        11715..12618
                     /label=PromGmUbi
     CDS             12639..12856
                     /codon_start=1
                     /label=GAL4 DNA binding domain (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=49.4"
                     /note="/other=CDS"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIF"
     CDS             12867..13100
                     /codon_start=1
                     /label=VP16 AD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             13107..13937
                     /codon_start=1
                     /label=GVG
                     /label=Nr3c1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=34.8"
                     /note="/other=CDS"
                     /translation="IQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLY
                     AGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLM
                     AFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEY
                     LCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLL
                     DSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
     terminator      13967..14263
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=46.3"
                     /note="/other=terminator"
     terminator      14392..14450
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=9.2"
                     /note="/other=terminator"
     protein_bind    14543..14560
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14561..14578
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14579..14597
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     protein_bind    14610..14627
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14628..14645
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    14646..14664
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     promoter        14711..14757
                     /label=minimal CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             14841..15611
                     /codon_start=1
                     /label=MiniTurboID
                     /label=birA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.0"
                     /note="/match_length=80.1"
                     /note="/other=CDS"
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    15618..15659
                     /label=V5 tag
     protein_bind    15678..15802
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        15827..15857
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        15881..15910
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             join(15911..16203,1..367)
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
       61 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      121 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
      181 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
      241 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
      301 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
      361 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
      421 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
      481 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
      541 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
      601 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
      661 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
      721 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
      781 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
      841 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
      901 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
      961 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     1021 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     1081 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     1141 attttacgtt tctcgttcag ctttcttgta caaagtggtc ctaggtgatt cggttcgagt
     1201 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     1261 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     1321 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     1381 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     1441 tcaaatcaag gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac
     1501 gggaaaaact atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat
     1561 atgcaaccta tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata
     1621 cgaagaagaa tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga
     1681 cgtaagcact gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac
     1741 atagaggaca catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa
     1801 tcttatcccc cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt
     1861 cctatataag gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat
     1921 tttctttgaa gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaac
     1981 aaaaatggat catgcgaccc tcgccatgat cctcgcgatc tggttcatca gcttccactt
     2041 catcaagctg ctgttctccc agcagaccac caagctgctt ccgccaggac caaagccgct
     2101 tccgatcatc ggcaacatcc ttgaggtggg caagaagccg catcggtcct tcgccaacct
     2161 cgccaagatt cacggcccac tcatttccct cagactcggc tctgtgacca ccatcgttgt
     2221 gtcctctgcc gacgtggcca aagagatgtt cctcaagaag gatcacccgc tctccaaccg
     2281 cacgatcccg aatagtgtta cagccggcga ccaccacaag ctcaccatgt cttggctccc
     2341 ggtgtctccg aagtggcgca acttccgcaa gattaccgcc gtgcatctgc tctccccaca
     2401 gagactcgat gcctgccaga cattcaggca cgccaaggtg cagcagctct acgagtacgt
     2461 tcaagagtgc gcccagaaag gccaggccgt ggatattggc aaggccgcct ttacgaccag
     2521 cctcaacctc ctcagcaagc tgttcttcag cgtcgagctg gcgcaccaca agtcccatac
     2581 cagccaagag ttcaaagagc tgatctggaa catcatggaa gatataggca agccgaacta
     2641 cgccgactac ttcccgattc tcggctgcgt tgacccatct ggcattagaa gaaggctcgc
     2701 ctgctccttc gacaagctga tcgccgtgtt ccagggcatc atctgcgaga gactcgcccc
     2761 agattcctcc accacaacta ccaccaccac cgacgacgtg ctcgatgtgc tcctccagct
     2821 gttcaagcag aacgagctga cgatgggcga gatcaaccac ctcctcgtgg acatcttcga
     2881 cgccggcacc gataccacat cctccacatt cgagtgggtg atgaccgagc tgatccgcaa
     2941 tccagagatg atggaaaagg cccaagagga aatcaagcag gtcctcggca aggacaagca
     3001 gatccaagag tccgacatca tcaacctgcc gtacctccag gcgatcatca aagagacact
     3061 ccgcctccat ccgccgaccg tgttcttgct cccaagaaag gccgacaccg atgtcgagct
     3121 gtacggctac atcgtgccga aggatgccca gatcctcgtg aacctctggg ccattggcag
     3181 ggacccaaac gcctggcaga acgccgatat tttcagccca gagcgcttca tcggctgcga
     3241 gatcgatgtt aagggccgcg atttcggcct ccttccattt ggcgctggcc gcagaatttg
     3301 cccaggcatg aatctcgcca tcaggatgct caccctcatg ctcgccacac tcctccagtt
     3361 cttcaactgg aagctcgaag gcgacatctc cccgaaggac ctcgacatgg acgagaagtt
     3421 cggcattgcg ctccaaaaga ccaagccgct caagctcatc ccgattccgc gctacggtag
     3481 cggagctacc aattttagcc tccttaagca ggcaggtgat gtagaagaga accccgggcc
     3541 tatgaagatg atgaacggcg aggacgccaa cgaccagatg atcaaagagt ccttcttcat
     3601 cacccacggc aacccgatcc tcaccgtcga ggatacacat ccgctcaggc cgttcttcga
     3661 gacatggcgc gagaagattt tctccaagaa gccgaaggcc atcctcatca tctccggcca
     3721 ctgggagaca gtgaagccaa ccgtgaacgc cgtgcacatc aacgacacca tccacgactt
     3781 cgacgactac ccagccgcca tgtaccagtt caagtaccca gctccaggcg agccagagct
     3841 tgcgagaaag gtggaagaga tcctcaagaa gtccgggttc gagacagccg agacagacca
     3901 aaagaggggc cttgatcacg gcgcctgggt tccactcatg ctcatgtatc cagaggcgga
     3961 catcccggtg tgccagctct cagttcagcc acatctcgac ggcacctacc actacaatct
     4021 cggcagagcc ctcgcgccgc tcaagaatga tggcgtgctc attattggct ccggcagcgc
     4081 cacacatcca ctcgatgaga caccgcacta cttcgatggt gttgcccctt gggccgctgc
     4141 cttcgattct tggcttagga aggccctcat caacggccgc ttcgaggaag tgaacatcta
     4201 cgagagcaag gccccgaact ggaagctcgc ccatccattt ccagagcact tctacccgct
     4261 ccacgttgtg ctcggcgctg ctggtgaaaa gtggaaggcc gagctgatcc actcctcctg
     4321 ggatcatggc acactttgcc acggctccta caagttcacc tccgccggat ccggagcaac
     4381 caactttagc ctgctcaagc aagcaggaga tgttgaggaa aatcctggcc ccatgaccgc
     4441 catcaagatg aacaccaacg gcgagggcga gacacagcac atcctcatga tcccgttcat
     4501 ggcgcagggc cacctcaggc catttctcga actcgccatg ttcctctaca agcgctccca
     4561 cgtgatcatc accctgctca caactccgct caatgccggc ttcctcaggc acctccttca
     4621 ccaccattcc tactcctcca gcggcatcag gatcgtcgag ctgccattca actccaccaa
     4681 ccacggactc ccaccgggca tcgagaacac cgataagctc acactcccgc tcgtggtgtc
     4741 cctcttccat tccaccatca gcctcgatcc gcacctccgc gattacatct ccaggcattt
     4801 cagcccagcc aggccaccac tctgcgtgat ccatgatgtg ttcctcggct gggttgacca
     4861 ggtggccaag gatgtgggct ctacaggcgt ggtgttcaca acaggcggcg cttatggcac
     4921 atccgcctac gtgtccatct ggaacgatct cccgcaccag aactactccg acgaccaaga
     4981 gttcccgctg ccaggcttcc cagagaacca taagttccgc aggtcccagc tccatcggtt
     5041 cctcagatat gccgacggct ccgacgattg gtccaagtat ttccagccgc agctccgcca
     5101 gtccatgaag tcttttggct ggctctgcaa ctccgtggaa gagatcgaga cactcggctt
     5161 ctccatcctc cgcaactaca ccaagctgcc gatctggggc atcggcccac ttattgcttc
     5221 cccagtgcag cactcctcct ccgacaacaa ttcaacaggc gccgagttcg tgcagtggct
     5281 cagcctcaaa gagccggact ccgtcctcta catctccttc ggctcccaga acacgatcag
     5341 cccgacgcag atgatggaac tcgctgctgg ccttgagtcc tccgagaagc cattcctctg
     5401 ggtgatcaga gccccgttcg gcttcgacat caacgaagag atgcgcccag agtggctgcc
     5461 agagggcttt gaggaacgca tgaaggtgaa gaaacagggc aagctcgtgt acaagctcgg
     5521 cccgcagctt gagatcctca accatgaatc catcggcggc tttctcaccc actgcggatg
     5581 gaacagcatc cttgagtctc ttcgcgaggg cgttccgatg cttggatggc cacttgctgc
     5641 cgagcaggcc tacaacctca agtacctcga agatgagatg ggcgtcgcgg ttgagcttgc
     5701 tagaggcctc gaaggcgaga tctccaaaga gaaggtcaag cgcatcgtcg agatgatcct
     5761 tgagcgcaac gagggctcca aaggctggga gatgaagaat cgcgccgtgg aaatgggcaa
     5821 aaagctcaag gacgccgtga acgaggaaaa agagctgaag ggctcctccg tgaaggcgat
     5881 cgacgatttc ctcgacgccg tcatgcaggc caaacttgag ccaagcctcc agtgagatgt
     5941 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     6001 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     6061 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     6121 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     6181 tctatgttac tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac
     6241 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     6301 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca
     6361 accacaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6901 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7081 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7141 aaacttggtc tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca
     7201 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
     7261 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     7321 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     7381 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     7441 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     7501 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     7561 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     7621 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     7681 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     7741 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     7801 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     7861 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     7921 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     7981 ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     8041 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     8101 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     8161 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     8221 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcca
     8281 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     8341 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     8401 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     8461 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     8521 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     8581 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
     8641 tcaaacatga gaattcttga aaatattgcg ctgccatttt tggggtgagg ccgttcgcgg
     8701 ccgaggggcg cagcccctgg gggatgggag ctcggtgcct tgatgtgggc gccggcggtc
     8761 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     8821 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     8881 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     8941 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     9001 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     9061 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     9121 gctatccaca ggaaagaagc tttttcgacc tttttcccct gctagggcaa tttgccctag
     9181 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     9241 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     9301 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     9361 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     9421 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     9481 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     9541 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     9601 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     9661 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     9721 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     9781 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     9841 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     9901 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     9961 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
    10021 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacgacgc
    10081 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
    10141 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
    10201 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
    10261 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
    10321 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
    10381 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
    10441 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
    10501 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
    10561 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
    10621 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
    10681 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
    10741 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
    10801 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
    10861 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
    10921 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
    10981 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
    11041 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
    11101 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
    11161 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
    11221 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
    11281 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
    11341 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
    11401 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
    11461 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    11521 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11581 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
    11641 acacattgcg gacgttttta atgtactggg gtggatgcag tgggccccac tctgtggaag
    11701 acaatgcctc cggagtaaca gataaagcga agcttgaagg tgcatgtgac tccgtcaaga
    11761 ttacgaaacc gccaactacc acgcaaattg caattctcaa tttcctagaa ggactctccg
    11821 aaaatgcatc caataccaaa tattacccgt gtcataggca ccaagtgaca ccatacatga
    11881 acacgcgtca caatatgact ggagaagggt tccacacctt atgctataaa acgccccaca
    11941 cccctcctcc ttccttcgca gttcaattcc aatatattcc attctctctg tgtatttccc
    12001 tacctctccc ttcaaggtta gtcgatttct tctgtttttc ttcttcgttc tttccatgaa
    12061 ttgtgtatgt tctttgatca atacgatgtt gatttgattg tgttttgttt ggtttcatcg
    12121 atcttcaatt ttcataatca gattcagctt ttattatctt tacaacaacg tccttaattt
    12181 gatgattctt taatcgtaga tttgctctaa ttagagcttt ttcatgtcag atccctttac
    12241 aacaagcctt aattgttgat tcattaatcg tagattaggg cttttttcat tgattacttc
    12301 agatccgtta aacgtaacca tagatcaggg ctttttcatg aattacttca gatccgttaa
    12361 acaacagcct tattttttat acttctgtgg tttttcaaga aattgttcag atccgttgac
    12421 aaaaagcctt attcgttgat tctatatcgt ttttcgagag atattgctca gatctgttag
    12481 caactgcctt gtttgttgat tctattgccg tggattaggg ttttttttca cgagattgct
    12541 tcagatccgt acttaagatt acgtaatgga ttttgattct gatttatctg tgattgttga
    12601 ctcgacagcg gccgcaccgg cgcgccatgg cagatccaat gaagctactg tcttctatcg
    12661 aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt
    12721 gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaagatctc
    12781 cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat
    12841 ttctactgat ttttccaagg tcgagcgccc ccccgaccga tgtcagcctg ggggacgagc
    12901 tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac gatttcgatc
    12961 tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac gactccgccc
    13021 cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc gatgcccttg
    13081 gaattgacga gtacggtggg gatccaattc agcaagccac tgcaggagtc tcacaagaca
    13141 cttcggaaaa tcctaacaaa acaatagttc ctgctgcatt accacagctc acccctacct
    13201 tggtgtcact gctggaggtg attgaacccg aggtgttgta tgcaggatat gatagctctg
    13261 ttccagattc agcatggaga attatgacca cactcaacat gttaggtggg cgtcaagtga
    13321 ttgcagcagt gaaatgggca aaggcgatac caggcttcag aaacttacac ctggatgacc
    13381 aaatgaccct gctacagtac tcatggatgt ttctcatggc atttgccctg ggttggagat
    13441 catacagaca atcaagtgga aacctgctct gctttgctcc tgatctgatt attaatgagc
    13501 agagaatgtc tctaccctgc atgtatgacc aatgtaaaca catgctgttt gtctcctctg
    13561 aattacaaag attgcaggta tcctatgaag agtatctctg tatgaaaacc ttactgcttc
    13621 tctcctcagt tcctaaggaa ggtctgaaga gccaagagtt atttgatgag attcgaatga
    13681 cttatatcaa agagctagga aaagccatcg tcaaaaggga agggaactcc agtcagaact
    13741 ggcaacggtt ttaccaactg acaaagcttc tggactccat gcatgaggtg gttgagaatc
    13801 tccttaccta ctgcttccag acatttttgg ataagaccat gagtattgaa ttcccagaga
    13861 tgttagctga aatcatcact aatcagatac caaaatattc aaatggaaat atcaaaaagc
    13921 ttctgtttca tcaaaaatga ctcgacctaa ctgagtaagc tagcttgttc gagtattatg
    13981 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
    14041 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
    14101 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
    14161 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
    14221 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac actagatccc caaacaagct
    14281 tgaaactgaa ggcgggaaac gacaatctga tcatgagcgg agaattaagg gagtcacgtt
    14341 atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac atatgaaaat
    14401 attttttaat gcattttatg acttgccaat tgattgacaa catgcatcaa tccgcggtta
    14461 tgactctctt aagagagtca tgaattcgag cgggaattga tcccccctcg acagcttgca
    14521 tgccggtcga ctctagagga tccgggtgac agccctccga cgggtgacag ccctccgacg
    14581 ggtgacagcc ctccgaattc tagaggatcc gggtgacagc cctccgacgg gtgacagccc
    14641 tccgacgggt gacagccctc cgaattcgag ctcagtaccc ggggatctgt cgacctcgat
    14701 cgagatcttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
    14761 gctgaagcta gtcgactcta gcctcgaatc cttaggtacc cttaacaaaa atggcaaggg
    14821 accccccagt cgcgaccatg attcctctgt taaacgcaaa gcagattctc gggcagttgg
    14881 atggggggag tgtggcggtc ctgccagtag tcgatagtac taatcaatat ctgctagata
    14941 gaataggtga gttaaaaagc ggagacgcgt gcatagccga atatcaacaa gcgggaagag
    15001 gctcacgagg aaggaagtgg tttagcccat tcggggcgaa tctgtatctt tctatgttct
    15061 ggcgtctgaa gagaggcccg gcagcgattg gattaggccc ggtcataggc atcgttatgg
    15121 ctgaggcttt aagaaagcta ggtgctgaca aagtgcgagt gaagtggcca aatgacttat
    15181 acttgcaaga caggaagcta gcagggattt tagtcgagct agccggtatc acaggtgacg
    15241 ctgcacaaat tgttataggg gccggtatca atgtggccat gcgtagagtg gaagaaagcg
    15301 tcgtgaacca aggatggatc actctacagg aggctggaat taatttggat cgaaacacct
    15361 tagctgcgat gctaattaga gaactgcgtg cggcactgga gctgttcgag caggaaggct
    15421 tggcccccta cctatcaagg tgggaaaagc tggataactt cattaaccgt ccagtgaaat
    15481 tgattattgg agataaggaa atctttggca tctcacgagg gattgacaag caaggggcat
    15541 tactgcttga acaggatggg gttattaagc cttggatggg aggggaaatc tccttaaggt
    15601 cagcggagaa ggcgtccggg aagccaatcc caaatcccct tctcggtctt gacagtaccg
    15661 gaggtggttc tggtaccaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
    15721 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
    15781 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
    15841 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
    15901 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
    15961 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
    16021 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
    16081 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
    16141 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
    16201 gca
//
