LOCUS       Exported               16185 bp ds-DNA     circular SYN 17-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..16185
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(882..974)
                     /label=PromTet
     rep_origin      complement(1249..1443)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1509..2582)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3011..3640)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3904..3928
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4028..4931
                     /label=PromGmUbi
     CDS             4952..5169
                     /codon_start=1
                     /label=GAL4 DNA binding domain (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=49.4"
                     /note="/other=CDS"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIF"
     CDS             5180..5413
                     /codon_start=1
                     /label=VP16 AD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             5420..6250
                     /codon_start=1
                     /label=GVG
                     /label=Nr3c1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.3"
                     /note="/match_length=34.8"
                     /note="/other=CDS"
                     /translation="IQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLY
                     AGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLM
                     AFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEY
                     LCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLL
                     DSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK"
     terminator      6280..6576
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=46.3"
                     /note="/other=terminator"
     terminator      6705..6763
                     /label=E9 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=9.2"
                     /note="/other=terminator"
     protein_bind    6856..6873
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6874..6891
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6892..6910
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     protein_bind    6923..6940
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6941..6958
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.3"
                     /note="/other=protein_bind"
     protein_bind    6959..6977
                     /label=UAS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.1"
                     /note="/other=protein_bind"
     promoter        7024..7070
                     /label=minimal CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    7114..7238
                     /label=attR1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        7263..7293
                     /label=lac UV5 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        7317..7346
                     /label=cat promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.1"
                     /note="/other=promoter"
     CDS             7347..8006
                     /codon_start=1
                     /label=CmR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             8185..8343
                     /codon_start=1
                     /label=ccdA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=73.6"
                     /note="/other=CDS"
                     /translation="YDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGS
                     FADENRGW"
     CDS             8348..8650
                     /codon_start=1
                     /label=ccdB
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(8694..8818)
                     /label=attR2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             8865..9635
                     /codon_start=1
                     /label=MiniTurboID
                     /label=birA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.0"
                     /note="/match_length=80.1"
                     /note="/other=CDS"
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    9642..9683
                     /label=V5 tag
     terminator      9687..9945
                     /label=TerRbsc
                     /note="TerRbsc"
     promoter        9946..10472
                     /label=PromCsVMV
                     /note="PromCmVsV"
     CDS             10483..14433
                     /label=RUBY
     terminator      14442..14694
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    14749..14773
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      complement(14896..15484)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    15692..15875
                     /label=TetC term
     CDS             complement(join(15876..16185,1..881))
                     /codon_start=1
                     /label=TcR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
       61 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      121 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
      181 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
      241 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
      301 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      361 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
      601 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      661 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
      721 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
      781 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      841 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      901 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      961 tgagaattct tgaaaatatt gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     1021 gcgcagcccc tgggggatgg gagctcggtg ccttgatgtg ggcgccggcg gtcgagtggc
     1081 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     1141 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     1201 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     1261 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     1321 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     1381 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     1441 acaggaaaga agctttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     1501 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     1561 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     1621 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     1681 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     1741 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     1801 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     1861 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     1921 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     1981 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     2041 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     2101 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     2161 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     2221 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     2281 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     2341 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacga cgcaccggct
     2401 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     2461 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     2521 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     2581 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     2641 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     2701 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     2761 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     2821 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     2881 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     2941 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     3001 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     3061 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     3121 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     3181 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     3241 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     3301 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     3361 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     3421 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     3481 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     3541 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     3601 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     3661 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     3721 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     3781 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggctgatgg
     3841 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3901 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     3961 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtgg aagacaatgc
     4021 ctccggagta acagataaag cgaagcttga aggtgcatgt gactccgtca agattacgaa
     4081 accgccaact accacgcaaa ttgcaattct caatttccta gaaggactct ccgaaaatgc
     4141 atccaatacc aaatattacc cgtgtcatag gcaccaagtg acaccataca tgaacacgcg
     4201 tcacaatatg actggagaag ggttccacac cttatgctat aaaacgcccc acacccctcc
     4261 tccttccttc gcagttcaat tccaatatat tccattctct ctgtgtattt ccctacctct
     4321 cccttcaagg ttagtcgatt tcttctgttt ttcttcttcg ttctttccat gaattgtgta
     4381 tgttctttga tcaatacgat gttgatttga ttgtgttttg tttggtttca tcgatcttca
     4441 attttcataa tcagattcag cttttattat ctttacaaca acgtccttaa tttgatgatt
     4501 ctttaatcgt agatttgctc taattagagc tttttcatgt cagatccctt tacaacaagc
     4561 cttaattgtt gattcattaa tcgtagatta gggctttttt cattgattac ttcagatccg
     4621 ttaaacgtaa ccatagatca gggctttttc atgaattact tcagatccgt taaacaacag
     4681 ccttattttt tatacttctg tggtttttca agaaattgtt cagatccgtt gacaaaaagc
     4741 cttattcgtt gattctatat cgtttttcga gagatattgc tcagatctgt tagcaactgc
     4801 cttgtttgtt gattctattg ccgtggatta gggttttttt tcacgagatt gcttcagatc
     4861 cgtacttaag attacgtaat ggattttgat tctgatttat ctgtgattgt tgactcgaca
     4921 gcggccgcac cggcgcgcca tggcagatcc aatgaagcta ctgtcttcta tcgaacaagc
     4981 atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa
     5041 gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaagat ctccgctgac
     5101 tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact
     5161 gatttttcca aggtcgagcg cccccccgac cgatgtcagc ctgggggacg agctccactt
     5221 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     5281 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     5341 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     5401 cgagtacggt ggggatccaa ttcagcaagc cactgcagga gtctcacaag acacttcgga
     5461 aaatcctaac aaaacaatag ttcctgctgc attaccacag ctcaccccta ccttggtgtc
     5521 actgctggag gtgattgaac ccgaggtgtt gtatgcagga tatgatagct ctgttccaga
     5581 ttcagcatgg agaattatga ccacactcaa catgttaggt gggcgtcaag tgattgcagc
     5641 agtgaaatgg gcaaaggcga taccaggctt cagaaactta cacctggatg accaaatgac
     5701 cctgctacag tactcatgga tgtttctcat ggcatttgcc ctgggttgga gatcatacag
     5761 acaatcaagt ggaaacctgc tctgctttgc tcctgatctg attattaatg agcagagaat
     5821 gtctctaccc tgcatgtatg accaatgtaa acacatgctg tttgtctcct ctgaattaca
     5881 aagattgcag gtatcctatg aagagtatct ctgtatgaaa accttactgc ttctctcctc
     5941 agttcctaag gaaggtctga agagccaaga gttatttgat gagattcgaa tgacttatat
     6001 caaagagcta ggaaaagcca tcgtcaaaag ggaagggaac tccagtcaga actggcaacg
     6061 gttttaccaa ctgacaaagc ttctggactc catgcatgag gtggttgaga atctccttac
     6121 ctactgcttc cagacatttt tggataagac catgagtatt gaattcccag agatgttagc
     6181 tgaaatcatc actaatcaga taccaaaata ttcaaatgga aatatcaaaa agcttctgtt
     6241 tcatcaaaaa tgactcgacc taactgagta agctagcttg ttcgagtatt atggcattgg
     6301 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     6361 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     6421 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     6481 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc
     6541 tctaatgacc gaagttaata tgaggagtaa aacactagat ccccaaacaa gcttgaaact
     6601 gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac gttatgaccc
     6661 ccgccgatga cgcgggacaa gccgttttac gtttggaact gacatatgaa aatatttttt
     6721 aatgcatttt atgacttgcc aattgattga caacatgcat caatccgcgg ttatgactct
     6781 cttaagagag tcatgaattc gagcgggaat tgatcccccc tcgacagctt gcatgccggt
     6841 cgactctaga ggatccgggt gacagccctc cgacgggtga cagccctccg acgggtgaca
     6901 gccctccgaa ttctagagga tccgggtgac agccctccga cgggtgacag ccctccgacg
     6961 ggtgacagcc ctccgaattc gagctcagta cccggggatc tgtcgacctc gatcgagatc
     7021 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaag
     7081 ctagtcgact ctagcctcga atccttaggt accacaagtt tgtacaaaaa agctgaacga
     7141 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     7201 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
     7261 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
     7321 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     7381 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     7441 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     7501 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     7561 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     7621 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     7681 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     7741 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     7801 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     7861 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     7921 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     7981 gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac
     8041 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     8101 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     8161 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     8221 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     8281 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     8341 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     8401 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     8461 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     8521 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     8581 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     8641 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
     8701 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
     8761 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtcc
     8821 taggcggagg tggttctgca agggaccccc cagtcgcgac catgattcct ctgttaaacg
     8881 caaagcagat tctcgggcag ttggatgggg ggagtgtggc ggtcctgcca gtagtcgata
     8941 gtactaatca atatctgcta gatagaatag gtgagttaaa aagcggagac gcgtgcatag
     9001 ccgaatatca acaagcggga agaggctcac gaggaaggaa gtggtttagc ccattcgggg
     9061 cgaatctgta tctttctatg ttctggcgtc tgaagagagg cccggcagcg attggattag
     9121 gcccggtcat aggcatcgtt atggctgagg ctttaagaaa gctaggtgct gacaaagtgc
     9181 gagtgaagtg gccaaatgac ttatacttgc aagacaggaa gctagcaggg attttagtcg
     9241 agctagccgg tatcacaggt gacgctgcac aaattgttat aggggccggt atcaatgtgg
     9301 ccatgcgtag agtggaagaa agcgtcgtga accaaggatg gatcactcta caggaggctg
     9361 gaattaattt ggatcgaaac accttagctg cgatgctaat tagagaactg cgtgcggcac
     9421 tggagctgtt cgagcaggaa ggcttggccc cctacctatc aaggtgggaa aagctggata
     9481 acttcattaa ccgtccagtg aaattgatta ttggagataa ggaaatcttt ggcatctcac
     9541 gagggattga caagcaaggg gcattactgc ttgaacagga tggggttatt aagccttgga
     9601 tgggagggga aatctcctta aggtcagcgg agaaggcgtc cgggaagcca atcccaaatc
     9661 cccttctcgg tcttgacagt acctgattcg gttcgagtat tatggcattg ggaaaactgt
     9721 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
     9781 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
     9841 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat
     9901 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcaaggt tccagaaggt
     9961 aattatccaa gatgtagcat caagaatcca atgtttacgg gaaaaactat ggaagtatta
    10021 tgtgagctca gcaagaagca gatcaatatg cggcacatat gcaacctatg ttcaaaaatg
    10081 aagaatgtac agatacaaga tcctatactg ccagaatacg aagaagaata cgtagaaatt
    10141 gaaaaagaag aaccaggcga agaaaagaat cttgaagacg taagcactga cgacaacaat
    10201 gaaaagaaga agataaggtc ggtgattgtg aaagagacat agaggacaca tgtaaggtgg
    10261 aaaatgtaag ggcggaaagt aaccttatca caaaggaatc ttatccccca ctacttatcc
    10321 ttttatattt ttccgtgtca tttttgccct tgagttttcc tatataagga accaagttcg
    10381 gcatttgtga aaacaagaaa aaatttggtg taagctattt tctttgaagt actgaggata
    10441 caacttcaga gaaatttgta agtttgtgat ccgcaaacaa aaatggatca tgcgaccctc
    10501 gccatgatcc tcgcgatctg gttcatcagc ttccacttca tcaagctgct gttctcccag
    10561 cagaccacca agctgcttcc gccaggacca aagccgcttc cgatcatcgg caacatcctt
    10621 gaggtgggca agaagccgca tcggtccttc gccaacctcg ccaagattca cggcccactc
    10681 atttccctca gactcggctc tgtgaccacc atcgttgtgt cctctgccga cgtggccaaa
    10741 gagatgttcc tcaagaagga tcacccgctc tccaaccgca cgatcccgaa tagtgttaca
    10801 gccggcgacc accacaagct caccatgtct tggctcccgg tgtctccgaa gtggcgcaac
    10861 ttccgcaaga ttaccgccgt gcatctgctc tccccacaga gactcgatgc ctgccagaca
    10921 ttcaggcacg ccaaggtgca gcagctctac gagtacgttc aagagtgcgc ccagaaaggc
    10981 caggccgtgg atattggcaa ggccgccttt acgaccagcc tcaacctcct cagcaagctg
    11041 ttcttcagcg tcgagctggc gcaccacaag tcccatacca gccaagagtt caaagagctg
    11101 atctggaaca tcatggaaga tataggcaag ccgaactacg ccgactactt cccgattctc
    11161 ggctgcgttg acccatctgg cattagaaga aggctcgcct gctccttcga caagctgatc
    11221 gccgtgttcc agggcatcat ctgcgagaga ctcgccccag attcctccac cacaactacc
    11281 accaccaccg acgacgtgct cgatgtgctc ctccagctgt tcaagcagaa cgagctgacg
    11341 atgggcgaga tcaaccacct cctcgtggac atcttcgacg ccggcaccga taccacatcc
    11401 tccacattcg agtgggtgat gaccgagctg atccgcaatc cagagatgat ggaaaaggcc
    11461 caagaggaaa tcaagcaggt cctcggcaag gacaagcaga tccaagagtc cgacatcatc
    11521 aacctgccgt acctccaggc gatcatcaaa gagacactcc gcctccatcc gccgaccgtg
    11581 ttcttgctcc caagaaaggc cgacaccgat gtcgagctgt acggctacat cgtgccgaag
    11641 gatgcccaga tcctcgtgaa cctctgggcc attggcaggg acccaaacgc ctggcagaac
    11701 gccgatattt tcagcccaga gcgcttcatc ggctgcgaga tcgatgttaa gggccgcgat
    11761 ttcggcctcc ttccatttgg cgctggccgc agaatttgcc caggcatgaa tctcgccatc
    11821 aggatgctca ccctcatgct cgccacactc ctccagttct tcaactggaa gctcgaaggc
    11881 gacatctccc cgaaggacct cgacatggac gagaagttcg gcattgcgct ccaaaagacc
    11941 aagccgctca agctcatccc gattccgcgc tacggtagcg gagctaccaa ttttagcctc
    12001 cttaagcagg caggtgatgt agaagagaac cccgggccta tgaagatgat gaacggcgag
    12061 gacgccaacg accagatgat caaagagtcc ttcttcatca cccacggcaa cccgatcctc
    12121 accgtcgagg atacacatcc gctcaggccg ttcttcgaga catggcgcga gaagattttc
    12181 tccaagaagc cgaaggccat cctcatcatc tccggccact gggagacagt gaagccaacc
    12241 gtgaacgccg tgcacatcaa cgacaccatc cacgacttcg acgactaccc agccgccatg
    12301 taccagttca agtacccagc tccaggcgag ccagagcttg cgagaaaggt ggaagagatc
    12361 ctcaagaagt ccgggttcga gacagccgag acagaccaaa agaggggcct tgatcacggc
    12421 gcctgggttc cactcatgct catgtatcca gaggcggaca tcccggtgtg ccagctctca
    12481 gttcagccac atctcgacgg cacctaccac tacaatctcg gcagagccct cgcgccgctc
    12541 aagaatgatg gcgtgctcat tattggctcc ggcagcgcca cacatccact cgatgagaca
    12601 ccgcactact tcgatggtgt tgccccttgg gccgctgcct tcgattcttg gcttaggaag
    12661 gccctcatca acggccgctt cgaggaagtg aacatctacg agagcaaggc cccgaactgg
    12721 aagctcgccc atccatttcc agagcacttc tacccgctcc acgttgtgct cggcgctgct
    12781 ggtgaaaagt ggaaggccga gctgatccac tcctcctggg atcatggcac actttgccac
    12841 ggctcctaca agttcacctc cgccggatcc ggagcaacca actttagcct gctcaagcaa
    12901 gcaggagatg ttgaggaaaa tcctggcccc atgaccgcca tcaagatgaa caccaacggc
    12961 gagggcgaga cacagcacat cctcatgatc ccgttcatgg cgcagggcca cctcaggcca
    13021 tttctcgaac tcgccatgtt cctctacaag cgctcccacg tgatcatcac cctgctcaca
    13081 actccgctca atgccggctt cctcaggcac ctccttcacc accattccta ctcctccagc
    13141 ggcatcagga tcgtcgagct gccattcaac tccaccaacc acggactccc accgggcatc
    13201 gagaacaccg ataagctcac actcccgctc gtggtgtccc tcttccattc caccatcagc
    13261 ctcgatccgc acctccgcga ttacatctcc aggcatttca gcccagccag gccaccactc
    13321 tgcgtgatcc atgatgtgtt cctcggctgg gttgaccagg tggccaagga tgtgggctct
    13381 acaggcgtgg tgttcacaac aggcggcgct tatggcacat ccgcctacgt gtccatctgg
    13441 aacgatctcc cgcaccagaa ctactccgac gaccaagagt tcccgctgcc aggcttccca
    13501 gagaaccata agttccgcag gtcccagctc catcggttcc tcagatatgc cgacggctcc
    13561 gacgattggt ccaagtattt ccagccgcag ctccgccagt ccatgaagtc ttttggctgg
    13621 ctctgcaact ccgtggaaga gatcgagaca ctcggcttct ccatcctccg caactacacc
    13681 aagctgccga tctggggcat cggcccactt attgcttccc cagtgcagca ctcctcctcc
    13741 gacaacaatt caacaggcgc cgagttcgtg cagtggctca gcctcaaaga gccggactcc
    13801 gtcctctaca tctccttcgg ctcccagaac acgatcagcc cgacgcagat gatggaactc
    13861 gctgctggcc ttgagtcctc cgagaagcca ttcctctggg tgatcagagc cccgttcggc
    13921 ttcgacatca acgaagagat gcgcccagag tggctgccag agggctttga ggaacgcatg
    13981 aaggtgaaga aacagggcaa gctcgtgtac aagctcggcc cgcagcttga gatcctcaac
    14041 catgaatcca tcggcggctt tctcacccac tgcggatgga acagcatcct tgagtctctt
    14101 cgcgagggcg ttccgatgct tggatggcca cttgctgccg agcaggccta caacctcaag
    14161 tacctcgaag atgagatggg cgtcgcggtt gagcttgcta gaggcctcga aggcgagatc
    14221 tccaaagaga aggtcaagcg catcgtcgag atgatccttg agcgcaacga gggctccaaa
    14281 ggctgggaga tgaagaatcg cgccgtggaa atgggcaaaa agctcaagga cgccgtgaac
    14341 gaggaaaaag agctgaaggg ctcctccgtg aaggcgatcg acgatttcct cgacgccgtc
    14401 atgcaggcca aacttgagcc aagcctccag tgagatgtgc agatcgttca aacatttggc
    14461 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
    14521 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
    14581 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
    14641 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatccgatga
    14701 taagctgtca aacatgaggg acacgaagtg gtttaaacta tcagtgtttg acaggatata
    14761 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
    14821 gtgaaaaggt ttatccgttc gtccatttgt atgtgccaac cacaggaacc gtaaaaaggc
    14881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    14941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    15001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    15061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    15121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    15181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    15241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    15301 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    15361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    15421 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    15481 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    15541 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    15601 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctaaaa
    15661 caattcatcc agtaaaatat aatcgaatat tatgcgcacc cgtggccagg acccaacgct
    15721 gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca
    15781 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt
    15841 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat
    15901 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc
    15961 aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg
    16021 atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca
    16081 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
    16141 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgcc
//
