LOCUS       XZ374_EF1a-IL2Rgsp-3xHA 7345 bp ds-DNA     circular     20-AUG-2024
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     misc_feature    215..1176
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1178..1332
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1378..1395
                     /label="homolog"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1397..1404
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    1405..1470
                     /label="hIL-2Rgamma Signal Peptide"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             1405..2367
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MLKPSLPFTSLLFLQLPLLGVGAAVYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAPAAGTLNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYMNCTWNSSSEPQPTNLTLHYWYKNSDNDKVQKCSHYLFSEEITSGCQLQKKEIHLYQTFVVQLQDPREPRRQATQMLKLQNLVIPWAPENLTLHKLSESQLELNWNNRFLNHCLEHLVQYRTDWDHSWTEQSVDYRHKFSLPSVDGQKRYTFRVRSRFNPLCGSAQHWSEWSHPIHWGSNTSKENPFLFALEAVVISVGSMGLIISLLCVYFWL"
     misc_feature    1471..1485
                     /label="homolog"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1471..1578
                     /label="3xHA"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
                     /note="ApEinfo_revcolor: #0080ff"
     misc_feature    1579..1584
                     /label="KpnI"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1579..1594
                     /label="homoolog"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1585..2304
                     /label="hIL-2Rgamma Ectodomain"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2305..2367
                     /label="hIL-2Rgamma Transmembrane"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2368..2373
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GT"
     CDS             2374..2382
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="AAA"
     misc_feature    2376..2390
                     /label="homolog"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     enhancer        2383..2442
                     /label="Traffic signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2383..3192
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="KSRITSEGEYIPLDQIDINVMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYGGGTGGGSMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYFCYENEV*"
     misc_feature    2406..2424
                     /label="PCR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2443..2793
                     /label="MCP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2511..2511
                     /label="G>A mutation"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2818..3168
                     /label="MCP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3004..3019
                     /label="homolog"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     enhancer        3169..3189
                     /label="ER export"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3198..3212
                     /label="homolog"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             3211..3243
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    3243..3245
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             3256..3273
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    3274..3276
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     polyA_signal    3295..3509
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     polyA_signal    3542..3565
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    3848..3895
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     gene            3903..4923
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    5055..5185
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     rep_origin      5568..6241
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(6386..7246)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(6386..7246)
                     /label="Translation 7246-6386"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(7247..7345)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
       61 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
      121 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
      181 aggcaaggct tgaccgacaa ttgcatgaag aatcGTGCCC GTCAGTGGGC AGAGCGCACA
      241 TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACCGG TGCCTAGAGA
      301 AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG
      361 GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG
      421 TTTGCCGCCA GAACACAGGT AAGTGCCGTG TGTGGTTCCC GCGGGCCTGG CCTCTTTACG
      481 GGTTATGGCC CTTGCGTGCC TTGAATTACT TCCACCTGGC TGCAGTACGT GATTCTTGAT
      541 CCCGAGCTTC GGGTTGGAAG TGGGTGGGAG AGTTCGAGGC CTTGCGCTTA AGGAGCCCCT
      601 TCGCCTCGTG CTTGAGTTGA GGCCTGGCCT GGGCGCTGGG GCCGCCGCGT GCGAATCTGG
      661 TGGCACCTTC GCGCCTGTCT CGCTGCTTTC GATAAGTCTC TAGCCATTTA AAATTTTTGA
      721 TGACCTGCTG CGACGCTTTT TTTCTGGCAA GATAGTCTTG TAAATGCGGG CCAAGATCTG
      781 CACACTGGTA TTTCGGTTTT TGGGGCCGCG GGCGGCGACG GGGCCCGTGC GTCCCAGCGC
      841 ACATGTTCGG CGAGGCGGGG CCTGCGAGCG CGGCCACCGA GAATCGGACG GGGGTAGTCT
      901 CAAGCTGGCC GGCCTGCTCT GGTGCCTGGC CTCGCGCCGC CGTGTATCGC CCCGCCCTGG
      961 GCGGCAAGGC TGGCCCGGTC GGCACCAGTT GCGTGAGCGG AAAGATGGCC GCTTCCCGGC
     1021 CCTGCTGCAG GGAGCTCAAA ATGGAGGACG CGGCGCTCGG GAGAGCGGGC GGGTGAGTCA
     1081 CCCACACAAA GGAAAAGGGC CTTTCCGTCC TCAGCCGTCG CTTCATGTGA CTCCACGGAG
     1141 TACCGGGCGC CGTCCAGGCA CCTCGATTAG TTCTCGcGCT TTTGGAGTAC GTCGTCTTTA
     1201 GGTTGGGGGG AGGGGTTTTA TGCGATGGAG TTTCCCCACA CTGAGTGGGT GGAGACTGAA
     1261 GTTAGGCCAG CTTGGCACTT GATGTAATTC TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA
     1321 TCTTGGTTCA TTCTCAAGCC TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG
     1381 TCGTGaagct ACTAGTtcgc caccATGTTG AAGCCATCAT TACCATTCAC ATCCCTCTTA
     1441 TTCCTGCAGC TGCCCCTGCT GGGAGTGGGG GCGGCCGTTT ACCCATACGA TGTTCCTGAC
     1501 TATGCGGGCT ATCCCTATGA CGTCCCGGAC TATGCAGGAT CCTATCCATA TGACGTTCCA
     1561 GATTACGCTC CGGCCGCCGG TACCCTGAAC ACGACAATTC TGACGCCCAA TGGGAATGAA
     1621 GACACCACAG CTGATTTCTT CCTGACCACT ATGCCCACTG ACTCCCTCAG TGTTTCCACT
     1681 CTGCCCCTCC CAGAGGTTCA GTGTTTTGTG TTCAATGTCG AGTACATGAA TTGCACTTGG
     1741 AACAGCAGCT CTGAGCCCCA GCCTACCAAC CTCACTCTGC ATTATTGGTA CAAGAACTCG
     1801 GATAATGATA AAGTCCAGAA GTGCAGCCAC TATCTATTCT CTGAAGAAAT CACTTCTGGC
     1861 TGTCAGTTGC AAAAAAAGGA GATCCACCTC TACCAAACAT TTGTTGTTCA GCTCCAGGAC
     1921 CCACGGGAAC CCAGGAGACA GGCCACACAG ATGCTAAAAC TGCAGAATCT GGTGATCCCC
     1981 TGGGCTCCAG AGAACCTAAC ACTTCACAAA CTGAGTGAAT CCCAGCTAGA ACTGAACTGG
     2041 AACAACAGAT TCTTGAACCA CTGTTTGGAG CACTTGGTGC AGTACCGGAC TGACTGGGAC
     2101 CACAGCTGGA CTGAACAATC AGTGGATTAT AGACATAAGT TCTCCTTGCC TAGTGTGGAT
     2161 GGGCAGAAAC GCTACACGTT TCGTGTTCGG AGCCGCTTTA ACCCACTCTG TGGAAGTGCT
     2221 CAGCATTGGA GTGAATGGAG CCACCCAATC CACTGGGGGA GCAATACTTC AAAAGAGAAT
     2281 CCTTTCCTGT TTGCATTGGA AGCCGTGGTT ATCTCTGTTG GCTCCATGGG ATTGATTATC
     2341 AGCCTTCTCT GTGTGTATTT CTGGCTGGGT ACCgcggccg ccaagagcag gatcaccagc
     2401 gagggcgagt acatccccct ggaccagatc gacatcaacg tgATGGCGAG CAACTTTACG
     2461 CAATTTGTGT TGGTTGACAA TGGTGGCACC GGAGACGTAA CTGTTGCGCC AAGCAATTTT
     2521 GCGAACGGGA TTGCAGAATG GATAAGTAGT AACAGCCGGA GTCAGGCATA TAAAGTCACA
     2581 TGCTCAGTTC GCCAAAGTTC CGCACAGAAC CGAAAATATA CCATAAAGGT AGAAGTGCCA
     2641 AAAGGGGCGT GGCGATCTTA TCTTAATATG GAGCTTACGA TCCCAATCTT TGCGACTAAT
     2701 TCAGACTGCG AGCTGATTGT CAAGGCCATG CAAGGTCTTT TGAAAGACGG TAACCCGATT
     2761 CCCTCTGCGA TCGCCGCCAA CTCAGGAATA TATGGTGGCG GAACCGGTGG CGGGTCCATG
     2821 GCCTCCAATT TTACACAGTT TGTGCTCGTG GACAACGGCG GCACTGGAGA CGTCACAGTA
     2881 GCTCCAAGTA ACTTTGCGAA TGGGATAGCC GAGTGGATCT CTTCCAATTC AAGATCACAA
     2941 GCCTATAAGG TGACTTGTAG TGTACGCCAA TCTAGTGCCC AGAACCGGAA GTATACCATT
     3001 AAAGTGGAGG TCCCCAAAGG TGCATGGCGC TCATACTTGA ATATGGAATT GACGATTCCG
     3061 ATATTTGCGA CCAATAGCGA CTGCGAGCTT ATTGTAAAAG CCATGCAAGG ACTTCTGAAA
     3121 GATGGCAACC CGATACCTTC CGCAATCGCT GCGAATAGCG GAATTTACtt ctgctacgag
     3181 aacgaggtgt gagccgctcg aggagggccc gaacaaaaac tcatctcaga agaggatctg
     3241 aatagcgccg tcgaccatca tcataatcat cattgagttt aaacccgctg atcagcctcg
     3301 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3361 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3421 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3481 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggagat ctatcgatgt
     3541 tctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     3601 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3661 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggtc cctttagggt
     3781 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3841 gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg
     3901 ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     3961 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     4021 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     4081 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     4141 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     4201 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     4261 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     4321 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     4381 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     4441 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     4501 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     4561 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     4621 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     4681 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     4741 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     4801 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     4861 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     4921 aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4981 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5041 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5101 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5161 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     5221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5521 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6061 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6181 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6241 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6301 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6361 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6421 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6481 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6541 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6601 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6661 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6721 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6781 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6841 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6901 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6961 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7021 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7081 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7141 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7201 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7261 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7321 tttagaaaaa taaacaaata ggggt
//