LOCUS       LSM105_CMV-TO-CX3CR1-(n 7464 bp ds-DNA     circular     19-JUN-2023
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        complement(1032..1130)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        1394..2021
                     /label="CMV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     enhancer        1394..2120
                     /label="CMV promoter (with TetOs)"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     misc_feature    1879..1879
                     /label="A"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     TATA_signal     1966..1972
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1982..2000
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    1982..2021
                     /label="TetR binding sites"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    2003..2021
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    2121..2122
                     /label="homolog"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2153..3217
                     /label="CX3CR1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2153..3242
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIFYSVIFAIGLVGNLLVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYDFFPSCDMRKDLRLALSVTETVAFSHCCLNPLIYAFAGEKFRRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSVLSSNFTYHTSDGDALLLLGTGALHLD"
     misc_feature    3230..4438
                     /label="hADAR2 cat domain"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3239..4524
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="DQTPSRQPIPSEGLQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNNKDDQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKARGQLRTKIESGQGTIPVRSNASIQAWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKITKPNVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSLTP*AARGGPEQKLISEEDLNSAVDHHHHHH"
     misc_feature    3243..2122
                     /label="LSM044"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3797..3799
                     /label="E488Q"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3836..3838
                     /label="T501A"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4439..4441
                     /label="Translation 4439-4441"
                     /translation="*"
     CDS             4460..4492
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4492..4494
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             4505..4522
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4523..4525
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     polyA_signal    4544..4758
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     polyA_signal    4791..4814
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5097..5144
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     gene            5152..6172
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    6304..6434
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     rep_origin      6817..26
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgtacgg
     1381 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     1441 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1501 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1561 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1621 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1681 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1741 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1801 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1861 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1921 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1981 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta
     2041 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2101 cgggaccgat ccagcctccg gaCTCTAGCG TTTAAACTTA AGCTTCGCCA CCATGGATCA
     2161 ATTTCCAGAA AGTGTGACTG AAAATTTTGA GTATGACGAC CTGGCAGAGG CCTGCTACAT
     2221 CGGCGATATT GTTGTCTTCG GCACTGTTTT CCTGAGCATT TTCTATTCCG TTATATTCGC
     2281 CATTGGCCTC GTTGGAAATC TTCTGGTGGT GTTCGCGCTC ACCAATAGTA AGAAGCCTAA
     2341 AAGCGTGACA GACATTTATC TTCTTAACCT CGCATTGTCA GACTTGTTAT TTGTCGCCAC
     2401 CCTGCCATTT TGGACCCACT ATTTAATTAA TGAGAAGGGA CTTCACAACG CGATGTGTAA
     2461 ATTTACTACA GCCTTCTTCT TCATAGGGTT CTTTGGGTCA ATCTTCTTTA TAACAGTCAT
     2521 ATCAATTGAT AGATATCTGG CCATCGTTCT GGCTGCCAAC AGCATGAATA ATCGGACAGT
     2581 GCAACATGGT GTAACTATTA GTCTGGGCGT ATGGGCCGCC GCTATTTTAG TGGCCGCTCC
     2641 CCAATTCATG TTTACCAAAC AAAAGGAAAA CGAATGCTTG GGAGATTATC CAGAAGTACT
     2701 GCAGGAAATT TGGCCCGTAC TCAGAAACGT GGAGACAAAC TTTCTTGGGT TTCTGTTGCC
     2761 ACTTCTGATT ATGTCCTATT GCTACTTTAG GATAATCCAA ACCCTCTTTA GCTGTAAGAA
     2821 TCACAAGAAA GCCAAAGCTA TCAAGCTCAT ATTACTGGTA GTAATTGTCT TCTTTCTCTT
     2881 CTGGACGCCT TATAATGTCA TGATTTTCTT GGAGACACTC AAGCTCTATG ATTTCTTTCC
     2941 ATCTTGCGAT ATGCGCAAGG ATTTGAGGCT TGCTCTGTCT GTAACAGAAA CAGTTGCTTT
     3001 CAGTCACTGC TGCTTAAACC CGTTGATCTA TGCATTTGCC GGAGAAAAGT TCAGGCGTTA
     3061 TCTCTACCAT CTTTATGGCA AGTGCCTTGC GGTGCTCTGC GGTCGGTCCG TCCATGTCGA
     3121 TTTCAGCTCC AGTGAAAGTC AAAGATCCAG ACACGGGAGC GTTTTGAGTT CCAACTTCAC
     3181 TTACCACACC TCTGATGGTG ATGCTCTGCT CCTGCTGGGT ACCGGTGCTT TGCACTTGGA
     3241 TCAAACGCCA TCTCGCCAGC CTATTCCCAG TGAGGGTCTT CAGCTGCATT TACCGCAGGT
     3301 TTTAGCTGAC GCTGTCTCAC GCCTGGTCCT GGGTAAGTTT GGTGACCTGA CCGACAACTT
     3361 CTCCTCCCCT CACGCTCGCA GAAAAGTGCT GGCTGGAGTC GTCATGACAA CAGGCACAGA
     3421 TGTTAAAGAT GCCAAGGTGA TAAGTGTTTC TACAGGAACA AAATGTATTA ATGGTGAATA
     3481 CATGAGTGAT CGTGGCCTTG CATTAAATGA CTGCCATGCA GAAATAATAT CTCGGAGATC
     3541 CTTGCTCAGA TTTCTTTATA CACAACTTGA GCTTTACTTA AATAACAAAG ATGATCAAAA
     3601 AAGATCCATC TTTCAGAAAT CAGAGCGAGG GGGGTTTAGG CTGAAGGAGA ATGTCCAGTT
     3661 TCATCTGTAC ATCAGCACCT CTCCCTGTGG AGATGCCAGA ATCTTCTCAC CACATGAGCC
     3721 AATCCTGGAA GAACCAGCAG ATAGACACCC AAATCGTAAA GCAAGAGGAC AGCTACGGAC
     3781 CAAAATAGAG TCTGGTCAGG GGACGATTCC AGTGCGCTCC AATGCGAGCA TCCAAGCGTG
     3841 GGACGGGGTG CTGCAAGGGG AGCGGCTGCT CACCATGTCC TGCAGTGACA AGATTGCACG
     3901 CTGGAACGTG GTGGGCATCC AGGGATCCCT GCTCAGCATT TTCGTGGAGC CCATTTACTT
     3961 CTCGAGCATC ATCCTGGGCA GCCTTTACCA CGGGGACCAC CTTTCCAGGG CCATGTACCA
     4021 GCGGATCTCC AACATAGAGG ACCTGCCACC TCTCTACACC CTCAACAAGC CTTTGCTCAG
     4081 TGGCATCAGC AATGCAGAAG CACGGCAGCC AGGGAAGGCC CCCAACTTCA GTGTCAACTG
     4141 GACGGTAGGC GACTCCGCTA TTGAGGTCAT CAACGCCACG ACTGGGAAGG ATGAGCTGGG
     4201 CCGCGCGTCC CGCCTGTGTA AGCACGCGTT GTACTGTCGC TGGATGCGTG TGCACGGCAA
     4261 GGTTCCCTCC CACTTACTAC GCTCCAAGAT TACCAAACCC AACGTGTACC ATGAGTCCAA
     4321 GCTGGCGGCA AAGGAGTACC AGGCCGCCAA GGCGCGTCTG TTCACAGCCT TCATCAAGGC
     4381 GGGGCTGGGG GCCTGGGTGG AGAAGCCCAC CGAGCAGGAC CAGTTCTCAC TCACGCCCtg
     4441 agccgctcga ggagggcccg aacaaaaact catctcagaa gaggatctga atagcgccgt
     4501 cgaccatcat catcatcatc attgagttta aacccgctga tcagcctcga ctgtgccttc
     4561 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4621 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4681 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4741 tagcaggcat gctggggatg cggtgggctc tatggagatc tatcgatgtt ctggggatgc
     4801 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4861 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4921 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4981 gttcgccggc tttccccgtc aagctctaaa tcgggggtcc ctttagggtt ccgatttagt
     5041 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     5101 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     5161 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     5221 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     5281 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     5341 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5401 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     5461 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     5521 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     5581 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     5641 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5701 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5761 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5821 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5881 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5941 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     6001 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     6061 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     6121 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     6181 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6241 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6301 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6361 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6421 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6481 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6541 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6601 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6661 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6721 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6781 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6901 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6961 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7021 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7081 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7141 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7201 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7261 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7321 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7381 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7441 acgcgcagaa aaaaaggatc tcaa
//