LOCUS       LSM068_CMV-TO-CX3CR1-AD 7551 bp ds-DNA     circular     21-OCT-2024
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        complement(1032..1130)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        1394..2021
                     /label="CMV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     enhancer        1394..2120
                     /label="CMV promoter (with TetOs)"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     misc_feature    1879..1879
                     /label="A"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     TATA_signal     1966..1972
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1982..2000
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    1982..2021
                     /label="TetR binding sites"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    2003..2021
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    2121..2122
                     /label="homolog"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2153..3304
                     /label="CX3CR1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2153..3329
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIFYSVIFAIGLVGNLLVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYDFFPSCDMRKDLRLALSVTETVAFSHCCLNPLIYAFAGEKFRRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSVLSSNFTYHTSDGDALLLLTGGRTPPSLGPQDESCTTASSSLAKDTSSGTGALHLD"
     misc_feature    3317..4525
                     /label="hADAR2 cat domain"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3326..4611
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="DQTPSRQPIPSEGLQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNNKDDQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKARGQLRTKIESGQGTIPVRSNASIQAWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKITKPNVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSLTP*AARGGPEQKLISEEDLNSAVDHHHHHH"
     misc_feature    3330..2122
                     /label="LSM044"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3884..3886
                     /label="E488Q"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3923..3925
                     /label="T501A"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4526..4528
                     /label="Translation 4526-4528"
                     /translation="*"
     CDS             4547..4579
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4579..4581
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             4592..4609
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4610..4612
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     polyA_signal    4631..4845
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     polyA_signal    4878..4901
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    5184..5231
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     gene            5239..6259
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    6391..6521
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     rep_origin      6904..26
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgtacgg
     1381 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     1441 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1501 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1561 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1621 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1681 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1741 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1801 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1861 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1921 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1981 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta
     2041 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2101 cgggaccgat ccagcctccg gaCTCTAGCG TTTAAACTTA AGCTTCGCCA CCATGGATCA
     2161 ATTTCCAGAA AGTGTGACTG AAAATTTTGA GTATGACGAC CTGGCAGAGG CCTGCTACAT
     2221 CGGCGATATT GTTGTCTTCG GCACTGTTTT CCTGAGCATT TTCTATTCCG TTATATTCGC
     2281 CATTGGCCTC GTTGGAAATC TTCTGGTGGT GTTCGCGCTC ACCAATAGTA AGAAGCCTAA
     2341 AAGCGTGACA GACATTTATC TTCTTAACCT CGCATTGTCA GACTTGTTAT TTGTCGCCAC
     2401 CCTGCCATTT TGGACCCACT ATTTAATTAA TGAGAAGGGA CTTCACAACG CGATGTGTAA
     2461 ATTTACTACA GCCTTCTTCT TCATAGGGTT CTTTGGGTCA ATCTTCTTTA TAACAGTCAT
     2521 ATCAATTGAT AGATATCTGG CCATCGTTCT GGCTGCCAAC AGCATGAATA ATCGGACAGT
     2581 GCAACATGGT GTAACTATTA GTCTGGGCGT ATGGGCCGCC GCTATTTTAG TGGCCGCTCC
     2641 CCAATTCATG TTTACCAAAC AAAAGGAAAA CGAATGCTTG GGAGATTATC CAGAAGTACT
     2701 GCAGGAAATT TGGCCCGTAC TCAGAAACGT GGAGACAAAC TTTCTTGGGT TTCTGTTGCC
     2761 ACTTCTGATT ATGTCCTATT GCTACTTTAG GATAATCCAA ACCCTCTTTA GCTGTAAGAA
     2821 TCACAAGAAA GCCAAAGCTA TCAAGCTCAT ATTACTGGTA GTAATTGTCT TCTTTCTCTT
     2881 CTGGACGCCT TATAATGTCA TGATTTTCTT GGAGACACTC AAGCTCTATG ATTTCTTTCC
     2941 ATCTTGCGAT ATGCGCAAGG ATTTGAGGCT TGCTCTGTCT GTAACAGAAA CAGTTGCTTT
     3001 CAGTCACTGC TGCTTAAACC CGTTGATCTA TGCATTTGCC GGAGAAAAGT TCAGGCGTTA
     3061 TCTCTACCAT CTTTATGGCA AGTGCCTTGC GGTGCTCTGC GGTCGGTCCG TCCATGTCGA
     3121 TTTCAGCTCC AGTGAAAGTC AAAGATCCAG ACACGGGAGC GTTTTGAGTT CCAACTTCAC
     3181 TTACCACACC TCTGATGGTG ATGCTCTGCT CCTGCTGACA GGCGGTCGCA CTCCCCCTAG
     3241 CCTCGGTCCA CAGGATGAGT CATGCACGAC CGCGAGTAGC TCCCTCGCCA AAGACACCTC
     3301 TTCCGGTACC GGTGCTTTGC ACTTGGATCA AACGCCATCT CGCCAGCCTA TTCCCAGTGA
     3361 GGGTCTTCAG CTGCATTTAC CGCAGGTTTT AGCTGACGCT GTCTCACGCC TGGTCCTGGG
     3421 TAAGTTTGGT GACCTGACCG ACAACTTCTC CTCCCCTCAC GCTCGCAGAA AAGTGCTGGC
     3481 TGGAGTCGTC ATGACAACAG GCACAGATGT TAAAGATGCC AAGGTGATAA GTGTTTCTAC
     3541 AGGAACAAAA TGTATTAATG GTGAATACAT GAGTGATCGT GGCCTTGCAT TAAATGACTG
     3601 CCATGCAGAA ATAATATCTC GGAGATCCTT GCTCAGATTT CTTTATACAC AACTTGAGCT
     3661 TTACTTAAAT AACAAAGATG ATCAAAAAAG ATCCATCTTT CAGAAATCAG AGCGAGGGGG
     3721 GTTTAGGCTG AAGGAGAATG TCCAGTTTCA TCTGTACATC AGCACCTCTC CCTGTGGAGA
     3781 TGCCAGAATC TTCTCACCAC ATGAGCCAAT CCTGGAAGAA CCAGCAGATA GACACCCAAA
     3841 TCGTAAAGCA AGAGGACAGC TACGGACCAA AATAGAGTCT GGTCAGGGGA CGATTCCAGT
     3901 GCGCTCCAAT GCGAGCATCC AAGCGTGGGA CGGGGTGCTG CAAGGGGAGC GGCTGCTCAC
     3961 CATGTCCTGC AGTGACAAGA TTGCACGCTG GAACGTGGTG GGCATCCAGG GATCCCTGCT
     4021 CAGCATTTTC GTGGAGCCCA TTTACTTCTC GAGCATCATC CTGGGCAGCC TTTACCACGG
     4081 GGACCACCTT TCCAGGGCCA TGTACCAGCG GATCTCCAAC ATAGAGGACC TGCCACCTCT
     4141 CTACACCCTC AACAAGCCTT TGCTCAGTGG CATCAGCAAT GCAGAAGCAC GGCAGCCAGG
     4201 GAAGGCCCCC AACTTCAGTG TCAACTGGAC GGTAGGCGAC TCCGCTATTG AGGTCATCAA
     4261 CGCCACGACT GGGAAGGATG AGCTGGGCCG CGCGTCCCGC CTGTGTAAGC ACGCGTTGTA
     4321 CTGTCGCTGG ATGCGTGTGC ACGGCAAGGT TCCCTCCCAC TTACTACGCT CCAAGATTAC
     4381 CAAACCCAAC GTGTACCATG AGTCCAAGCT GGCGGCAAAG GAGTACCAGG CCGCCAAGGC
     4441 GCGTCTGTTC ACAGCCTTCA TCAAGGCGGG GCTGGGGGCC TGGGTGGAGA AGCCCACCGA
     4501 GCAGGACCAG TTCTCACTCA CGCCCtgagc cgctcgagga gggcccgaac aaaaactcat
     4561 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac
     4621 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4681 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4741 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4801 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4861 ggagatctat cgatgttctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4921 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4981 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5041 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5101 ggggtccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5161 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt
     5221 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     5281 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     5341 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     5401 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     5461 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     5521 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     5581 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     5641 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     5701 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     5761 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     5821 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     5881 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     5941 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     6001 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     6061 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     6121 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     6181 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     6241 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat
     6301 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6361 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     6421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6481 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     6541 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6601 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6661 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6721 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6841 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6901 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7321 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7381 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7441 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca a
//