LOCUS       XZ110_CMV-TO-signal-B2A 7800 bp ds-DNA     circular     23-FEB-2023
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     polyA_signal    851..981
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     rep_origin      1364..2037
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(2182..3042)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        complement(3043..3141)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        3405..4032
                     /label="CMV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     enhancer        3405..4131
                     /label="CMV promoter (with TetOs)"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     misc_feature    3890..3890
                     /label="A"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     TATA_signal     3977..3983
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3993..4011
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    3993..4032
                     /label="TetR binding sites"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    4014..4032
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    4136..4152
                     /label="homolog"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4156..4163
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    4157..4163
                     /label="startt"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             4164..6788
                     /label="Translation 4164-6788"
                     /translation="MKTIIALSYIFCLVFADYKDDDDASGGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLLAGGRTPPSLGPQDESCTTASSSLAKDTSSGTGALHLDQTPSRQPIPSEGLQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNNKDDQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKARGQLRTKIESGQGTIPVRSNASIQAWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKITKPNVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSLTP*"
     misc_feature    4167..4238
                     /label="Signal/Flag"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4227..4241
                     /label="homolog"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4242..5477
                     /label="hB2AR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5478..5492
                     /label="homolog"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5484..5564
                     /label="V2 tail"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5565..5573
                     /label="short linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5570..5570
                     /label="*"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5570..5584
                     /label="homolog"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    5577..6785
                     /label="hADAR2 cat domain"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    6144..6146
                     /label="E488Q"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    6183..6185
                     /label="T501A"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             6786..6788
                     /label="Translation 6786-6788"
                     /translation="*"
     CDS             6807..6839
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    6839..6841
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             6852..6869
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    6870..6872
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     polyA_signal    6891..7105
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     polyA_signal    7138..7161
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    7444..7491
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     gene            7499..719
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
       61 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
      121 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
      181 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
      241 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
      301 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
      361 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
      421 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
      481 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
      541 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
      601 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
      661 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
      721 gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
      781 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
      841 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      961 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     1021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1861 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2161 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2221 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2281 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2341 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2461 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2521 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2581 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2641 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2761 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2821 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2881 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2941 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3061 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3121 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     3181 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     3241 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     3301 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     3361 agggttaggc gttttgcgct gcttcgtacg ggccagatat acgcgttgac attgattatt
     3421 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     3481 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     3541 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     3601 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     3661 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     3721 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     3781 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     3841 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     3901 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     3961 tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag atctccctat
     4021 cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     4081 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc
     4141 gtttaaactt aagcttcgcc accatgaaga cgatcatcgc cctgagctac atcttctgcc
     4201 tggtattcgc cgactacaag gacgatgatg acgccagcGG Cgggcaaccc gggaacggca
     4261 gcgccttctt gctggcaccc aatagaagcc atgcgccgga ccacgacgtc acgcagcaaa
     4321 gggacgaggt gtgggtggtg ggcatgggca tcgtcatgtc tctcatcgtc ctggccatcg
     4381 tgtttggcaa tgtgctggtc atcacagcca ttgccaagtt cgagcgtctg cagacggtca
     4441 ccaactactt catcacttca ctggcctgtg ctgatctggt catgggcctg gcagtggtgc
     4501 cctttggggc cgcccatatt cttatgaaaa tgtggacttt tggcaacttc tggtgcgagt
     4561 tttggacttc cattgatgtg ctgtgcgtca cggccagcat tgagaccctg tgcgtgatcg
     4621 cagtggatcg ctactttgcc attacttcac ctttcaagta ccagagcctg ctgaccaaga
     4681 ataaggcccg ggtgatcatt ctgatggtgt ggattgtgtc aggccttacc tccttcttgc
     4741 ccattcagat gcactggtat cgggccaccc accaggaagc catcaactgc tatgccaatg
     4801 agacctgctg tgacttcttc acgaaccaag cctatgccat tgcctcttcc atcgtgtcct
     4861 tctacgttcc cctggtgatc atggtcttcg tctactccag ggtctttcag gaggccaaaa
     4921 ggcagctcca gaagattgac aaatctgagg gccgcttcca tgtccagaac cttagccagg
     4981 tggagcagga tgggcggacg gggcatggac tccgcagatc ttccaagttc tgcttgaagg
     5041 agcacaaagc cctcaagacg ttaggcatca tcatgggcac tttcaccctc tgctggctgc
     5101 ccttcttcat cgttaacatt gtgcatgtga tccaggataa cctcatccgt aaggaagttt
     5161 acatcctcct aaattggata ggctatgtca attctggttt caatcccctt atctactgcc
     5221 ggagcccaga tttcaggatt gccttccagg agcttctgtg cctgcgcagg tcttctttga
     5281 aggcctatgg gaatggctac tccagcaacg gcaacacagg ggagcagagt ggatatcacg
     5341 tggaacagga gaaagaaaat aaactgctgt gtgaagacct cccaggcacg gaagactttg
     5401 tgggccatca aggtactgtg cctagcgata acattgattc acaagggagg aattgtagta
     5461 caaatgactc actgctgGCC ggtggacgca ccccacccag cctgggtccc caagatgagt
     5521 cctgcaccac cgccagctcc tccctggcca aggacacttc atcgGGTACC GGTGCTTTGC
     5581 ACTTGGATCA AACGCCATCT CGCCAGCCTA TTCCCAGTGA GGGTCTTCAG CTGCATTTAC
     5641 CGCAGGTTTT AGCTGACGCT GTCTCACGCC TGGTCCTGGG TAAGTTTGGT GACCTGACCG
     5701 ACAACTTCTC CTCCCCTCAC GCTCGCAGAA AAGTGCTGGC TGGAGTCGTC ATGACAACAG
     5761 GCACAGATGT TAAAGATGCC AAGGTGATAA GTGTTTCTAC AGGAACAAAA TGTATTAATG
     5821 GTGAATACAT GAGTGATCGT GGCCTTGCAT TAAATGACTG CCATGCAGAA ATAATATCTC
     5881 GGAGATCCTT GCTCAGATTT CTTTATACAC AACTTGAGCT TTACTTAAAT AACAAAGATG
     5941 ATCAAAAAAG ATCCATCTTT CAGAAATCAG AGCGAGGGGG GTTTAGGCTG AAGGAGAATG
     6001 TCCAGTTTCA TCTGTACATC AGCACCTCTC CCTGTGGAGA TGCCAGAATC TTCTCACCAC
     6061 ATGAGCCAAT CCTGGAAGAA CCAGCAGATA GACACCCAAA TCGTAAAGCA AGAGGACAGC
     6121 TACGGACCAA AATAGAGTCT GGTCAGGGGA CGATTCCAGT GCGCTCCAAT GCGAGCATCC
     6181 AAGCGTGGGA CGGGGTGCTG CAAGGGGAGC GGCTGCTCAC CATGTCCTGC AGTGACAAGA
     6241 TTGCACGCTG GAACGTGGTG GGCATCCAGG GATCCCTGCT CAGCATTTTC GTGGAGCCCA
     6301 TTTACTTCTC GAGCATCATC CTGGGCAGCC TTTACCACGG GGACCACCTT TCCAGGGCCA
     6361 TGTACCAGCG GATCTCCAAC ATAGAGGACC TGCCACCTCT CTACACCCTC AACAAGCCTT
     6421 TGCTCAGTGG CATCAGCAAT GCAGAAGCAC GGCAGCCAGG GAAGGCCCCC AACTTCAGTG
     6481 TCAACTGGAC GGTAGGCGAC TCCGCTATTG AGGTCATCAA CGCCACGACT GGGAAGGATG
     6541 AGCTGGGCCG CGCGTCCCGC CTGTGTAAGC ACGCGTTGTA CTGTCGCTGG ATGCGTGTGC
     6601 ACGGCAAGGT TCCCTCCCAC TTACTACGCT CCAAGATTAC CAAACCCAAC GTGTACCATG
     6661 AGTCCAAGCT GGCGGCAAAG GAGTACCAGG CCGCCAAGGC GCGTCTGTTC ACAGCCTTCA
     6721 TCAAGGCGGG GCTGGGGGCC TGGGTGGAGA AGCCCACCGA GCAGGACCAG TTCTCACTCA
     6781 CGCCCtgagc cgctcgagga gggcccgaac aaaaactcat ctcagaagag gatctgaata
     6841 gcgccgtcga ccatcatcat catcatcatt gagtttaaac ccgctgatca gcctcgactg
     6901 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6961 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7021 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7081 aagacaatag caggcatgct ggggatgcgg tgggctctat ggagatctat cgatgttctg
     7141 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     7201 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7261 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7321 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggtccctt tagggttccg
     7381 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac
     7441 ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa
     7501 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     7561 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     7621 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     7681 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     7741 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
//