LOCUS       LSM052_CMV-TO-CCR6-PCP_ 7242 bp ds-DNA     circular     07-JUN-2023
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        complement(1032..1130)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        1394..2021
                     /label="CMV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     enhancer        1394..2120
                     /label="CMV promoter (with TetOs)"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     misc_feature    1879..1879
                     /label="A"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     TATA_signal     1966..1972
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1982..2000
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    1982..2021
                     /label="TetR binding sites"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    2003..2021
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    2121..2140
                     /label="homolog"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2128..3464
                     /label="from xz032"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2144..3442
                     /label="from XZ032"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2145..2152
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    2146..2152
                     /label="startt"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2153..4219
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MKTIIALSYIFCLVFADYKDDDDASIDMSGESMNFSDVFDSSEDYFVSVNTSYYSVDSEMLLCSLQEVRQFSRLFVPIAYSLICVFGLLGNILVVITFAFYKKARSMTDVYLLNMAIADILFVLTLPFWAVSHATGAWVFSNATCKLLKGIYAINFNCGMLLLTCISMDRYIAIVQATKSFRLRSRTLPRSKIICLVVWGLSVIISSSTFVFNQKYNTQGSDVCEPKYQTVSEPIRWKLLMLGLELLFGFFIPLMFMIFCYTFIVKTLVQAQNSKRHKAIRVIIAVVLVFLACQIPHNMVLLVTAANLGKMNRSCQSEKLIGYTKTVTEVLAFLHCCLNPVLYAFIGQKFRNYFLKILKDLWCVRRKYKSSGFSCAGRYSENISRQTSETADNDNASSFTMTGGRTPPSLGPQDESCTTASSSLAKDTSSTGSGLASKTIVLSVGEATRTLTEIQSTADRQIFEEKVGPLVGRLRLTASLRQNGAKTAYRVNLKLDQADVVDSGLPKVRYTQVWSHDVTIVANSTEASRKSLYDLTKSLVATSQVEDLVVNLVPLGRGGGSGGGTLASKTIVLSVGEATRTLTEIQSTADRQIFEEKVGPLVGRLRLTASLRQNGAKTAYRVNLKLDQADVVDSGLPKVRYTQVWSHDVTIVANSTEASRKSLYDLTKSLVATSQVEDLVVNLVPLGR*"
     misc_feature    2156..2227
                     /label="Signal/Flag"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2234..3355
                     /label="CCR6"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="ApEinfo_revcolor: #ffef86"
     misc_feature    3339..3355
                     /label="homolog"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3362..3442
                     /label="V2 tail"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3443..2144
                     /label="from LSM040"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3443..3444
                     /label="homologous"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3443..3454
                     /label="short linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3445..3445
                     /label="*"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     One G missin... 3446..3446
                     /label="Mistake"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3455..3823
                     /label="PCP"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3848..4216
                     /label="PCP"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             4238..4270
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4270..4272
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             4283..4300
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4301..4303
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     polyA_signal    4322..4536
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     polyA_signal    4569..4592
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    4875..4922
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     gene            4930..5950
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    6082..6212
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     rep_origin      6595..26
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgtacgg
     1381 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     1441 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1501 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1561 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1621 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1681 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1741 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1801 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1861 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1921 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1981 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta
     2041 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2101 cgggaccgat ccagcctccg gactctagcg tttaaactta agcttcgcca ccatgaagac
     2161 gatcatcgcc ctgagctaca tcttctgcct ggtattcgcc gactacaagg acgatgatga
     2221 cgccagcatc gatATGAGCG GAGAGAGCAT GAACTTCAGT GATGTGTTCG ATTCCAGTGA
     2281 GGACTACTTC GTGTCCGTCA ATACATCCTA CTACAGCGTA GATTCTGAGA TGCTGCTTTG
     2341 CTCCCTGCAG GAGGTGCGCC AGTTCTCCCG ACTCTTCGTT CCCATCGCGT ATTCACTCAT
     2401 CTGCGTCTTT GGCCTCCTGG GGAATATCTT GGTAGTCATC ACATTTGCTT TTTATAAAAA
     2461 GGCCAGATCC ATGACAGACG TCTACCTGCT TAATATGGCC ATTGCAGACA TCCTGTTCGT
     2521 CCTGACGCTC CCATTCTGGG CTGTGAGTCA CGCTACCGGG GCCTGGGTGT TCAGTAATGC
     2581 AACCTGCAAG CTGTTGAAGG GAATTTATGC CATCAACTTT AATTGCGGTA TGCTGCTGCT
     2641 GACTTGCATA AGTATGGACA GATACATCGC CATAGTACAG GCAACAAAAT CCTTCAGACT
     2701 GAGGTCTCGA ACGCTCCCTA GGTCTAAAAT CATTTGTCTT GTTGTATGGG GCTTGAGCGT
     2761 GATTATTTCC AGCAGTACTT TCGTGTTTAA CCAGAAGTAC AACACGCAGG GCAGCGATGT
     2821 GTGTGAGCCC AAGTACCAGA CGGTATCTGA GCCTATCAGG TGGAAGCTGT TGATGCTGGG
     2881 TTTGGAATTG CTGTTCGGCT TTTTTATCCC ACTCATGTTT ATGATTTTTT GTTATACCTT
     2941 CATCGTGAAG ACCCTGGTCC AGGCACAAAA CTCTAAGCGA CACAAGGCCA TCCGGGTAAT
     3001 CATCGCTGTT GTGCTGGTAT TTCTGGCTTG CCAGATCCCT CATAATATGG TCCTTCTGGT
     3061 AACGGCAGCC AACCTGGGCA AAATGAATCG CTCATGTCAG TCAGAAAAGC TTATCGGGTA
     3121 CACCAAAACG GTTACGGAGG TTCTGGCGTT CCTGCATTGT TGCCTTAACC CTGTGCTGTA
     3181 CGCATTTATA GGTCAGAAGT TTCGAAACTA CTTCCTGAAG ATTCTCAAAG ACCTCTGGTG
     3241 TGTAAGAAGA AAGTATAAAA GTAGTGGATT TTCATGCGCA GGACGCTACT CCGAAAACAT
     3301 TTCCAGACAG ACCAGTGAGA CGGCCGATAA CGATAACGCC TCCTCCTTTA CCATGaccgg
     3361 tggacgcacc ccacccagcc tgggtcccca agatgagtcc tgcaccaccg ccagctcctc
     3421 cctggccaag gacacttcat cgACCgGATC CggcCTAGCT TCTAAAACAA TAGTGTTGTC
     3481 TGTAGGAGAA GCCACCAGAA CCCTCACGGA GATCCAAAGC ACAGCGGATA GACAAATTTT
     3541 CGAAGAGAAA GTGGGACCGC TTGTCGGAAG ACTCAGGTTG ACAGCTTCCC TGCGGCAGAA
     3601 TGGCGCTAAG ACCGCTTATA GGGTGAATCT CAAACTTGAT CAAGCAGATG TTGTTGATAG
     3661 CGGCTTGCCC AAAGTACGAT ATACTCAAGT TTGGTCACAT GATGTGACAA TCGTTGCAAA
     3721 CTCAACGGAA GCGAGTAGGA AAAGCCTTTA TGATCTCACT AAGTCACTCG TCGCCACCTC
     3781 TCAAGTTGAG GACCTTGTCG TCAATTTGGT GCCTCTTGGT CGGGGAGGCG GTTCCGGCGG
     3841 GGGTACCCTT GCCAGTAAGA CCATCGTGTT GAGCGTGGGA GAGGCAACCC GCACACTGAC
     3901 AGAAATCCAG TCAACAGCAG ACAGACAGAT TTTTGAGGAG AAAGTGGGTC CGCTTGTCGG
     3961 GCGGCTGCGG CTGACGGCTT CATTGCGACA GAACGGAGCC AAAACTGCCT ATCGAGTCAA
     4021 CCTTAAACTC GATCAAGCGG ATGTTGTGGA TTCCGGTCTG CCAAAAGTAC GGTACACGCA
     4081 GGTGTGGTCC CATGACGTGA CCATTGTGGC GAACAGTACC GAGGCGTCTC GCAAAAGCCT
     4141 TTACGATTTG ACCAAATCAC TCGTTGCAAC TTCACAGGTG GAAGACCTGG TTGTCAATCT
     4201 TGTACCTTTG GGCAGGtgag ccgctcgagg agggcccgaa caaaaactca tctcagaaga
     4261 ggatctgaat agcgccgtcg accatcatca tcatcatcat tgagtttaaa cccgctgatc
     4321 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4381 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4441 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4501 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggagatcta
     4561 tcgatgttct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4621 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4681 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4741 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggtccct
     4801 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4861 ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact
     4921 tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     4981 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     5041 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     5101 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     5161 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     5221 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     5281 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     5341 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     5401 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     5461 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     5521 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     5581 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     5641 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     5701 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     5761 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     5821 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     5881 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     5941 gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6001 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6061 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6121 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6181 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6241 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6601 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6661 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6721 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6781 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6901 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6961 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7021 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7081 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7141 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aa
//