LOCUS       LSM059_CMV-TO-bArrestin 7707 bp ds-DNA     circular     15-APR-2025
DEFINITION  .
COMMENT     LOCUS       CMV-TRE+H2B+Ceru        6284 bp    DNA     circular UNA
            02-AUG-2012 DEFINITION  Concatenation of 4 sequences. ACCESSION
            VERSION KEYWORDS    . SOURCE      .   ORGANISM               .
FEATURES             Location/Qualifiers
     CDS             complement(171..1031)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(296..314)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        complement(1032..1130)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1126..1142
                     /label="AmpR_seq_F"
                     /note="sequence: ggggttccgcgcacatt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1243..1263
                     /label="FlpInF1"
                     /note="sequence: agtatctgctccctgcttgtg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1307..1324
                     /label="FlpInF"
                     /note="sequence: aacaaggcaaggcttgac"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1318..1337
                     /label="FlpInF2"
                     /note="sequence: gcttgaccgacaattgcatg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1322..1344)
                     /label="EKo152.4"
                     /note="sequence: gattcttcatgcaattgtcggtc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1378..1394
                     /label="EKo279.1"
                     /note="sequence: cgggccagatatacgcg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1379..1425
                     /label="EKo272.1"
                     /note="sequence: gggccagatatacgcgttGACATTGATTATTGACTAGTTATTAATAG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        1394..2021
                     /label="CMV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     enhancer        1394..2120
                     /label="CMV promoter (with TetOs)"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     primer          1473..1495
                     /label="EK_CMVFa"
                     /note="sequence: cgttacataacttacggtaaatg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1549..1568
                     /label="EK_CMVFb"
                     /note="sequence: tatgttcccatagtaacgcc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1625..1645
                     /label="EK_CMVFc"
                     /note="sequence: tggcagtacatcaagtgtatc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1701..1719
                     /label="EK_CMVFd"
                     /note="sequence: ttatgcccagtacatgacc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1777..1794
                     /label="EK_CMVFe"
                     /note="sequence: gtgatgcggttttggcag"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1879..1879
                     /label="A"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          1931..1951
                     /label="CMV-Forward Genewiz"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     TATA_signal     1966..1972
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1982..2000
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    1982..2021
                     /label="TetR binding sites"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     misc_feature    2003..2021
                     /label="TetO"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     primer          2082..2102
                     /label="CMVF1"
                     /note="sequence: tgacctccatagaagacaccg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2103..2118
                     /label="ToFlpF"
                     /note="sequence: GGACCGATCCAGCCTC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2120..2144
                     /label="XZp025.1"
                     /note="sequence: ggactctagcgtttaaacttaagct"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2121..2140
                     /label="homolog"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2121..3490
                     /label="ARRB2_PCR-XZ024"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2125..2152
                     /label="EKbF"
                     /note="sequence: ctagcgtttaaacttaagcttcgccacc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2145..2152
                     /label="Kozak"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     misc_feature    2148..2152
                     /label="start"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2153..3379
                     /label="ARRB2"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             2153..3472
                     /label="Translation 2153-3472"
                     /translation="MGKKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIATYQAFPPVPNPPRPPTRLQDRLLRKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSLEEKSHKRNSVRLVIRKVQFAPEKPGPQPSAETTRHFLMSDRSLHLEASLDKELYYHGEPLNVNVHVTNNSTKTVKKIKVSVRQYADICLFSTAQYKCPVAQLEQDDQVSPSSTFCKVYTITPLLSDNREKRGLALDGKLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHIPLPRPQSAAPETDVPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDYDDQLCGTSGGSGSGAGSGSSGSETPGTSESATPESA"
     misc_feature    2159..2161
                     /label="E->K"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2588..2607
                     /label="ARRB2-seq-F1"
                     /note="sequence: gagattcgagccttctgtgc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3062..3080
                     /label="ARRB2-seq-F2"
                     /note="sequence: agcaccatcgtgaaggagg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3311..3340
                     /label="NES"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3359..3379
                     /label="PCR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3359..3384)
                     /label="oLSM0114"
                     /note="sequence: GTACCgcagagttgatcatcatagtc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          3359..3397
                     /label="oLSM0112"
                     /note="sequence: gactatgatgatcaactctgcGGTACCTCAGGCGGTTCC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3380..3469
                     /label="GS+XTEN linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3385..3385
                     /label="*"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(3451..3485)
                     /label="XZp023.4"
                     /note="sequence: GATCCAAGTGCAAAGCGCTCTCTGGCGTTGCTGAC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3467..3472
                     /label="Translation 3467-3472"
                     /translation="SA"
     misc_feature    3469..3469
                     /label="*"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3473..3491
                     /label="EKo330.4"
                     /note="sequence: TTGCACTTGGATCAAACGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3473..4681
                     /label="hADAR2 cat domain"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3473..4702
                     /label="Translation 3473-4702"
                     /translation="LHLDQTPSRQPIPSEGLQLHLPQVLADAVSRLVLGKFGDLTDNFSSPHARRKVLAGVVMTTGTDVKDAKVISVSTGTKCINGEYMSDRGLALNDCHAEIISRRSLLRFLYTQLELYLNNKDDQKRSIFQKSERGGFRLKENVQFHLYISTSPCGDARIFSPHEPILEEPADRHPNRKARGQLRTKIESGQGTIPVRSNASIQAWDGVLQGERLLTMSCSDKIARWNVVGIQGSLLSIFVEPIYFSSIILGSLYHGDHLSRAMYQRISNIEDLPPLYTLNKPLLSGISNAEARQPGKAPNFSVNWTVGDSAIEVINATTGKDELGRASRLCKHALYCRWMRVHGKVPSHLLRSKITKPNVYHESKLAAKEYQAAKARLFTAFIKAGLGAWVEKPTEQDQFSLTP*AARGGP"
     misc_feature    3473..4721
                     /label="hADAR2_PCR-XZ030"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3537..3555)
                     /label="XZ046-test-R"
                     /note="sequence: GCGTCAGCTAAAACCTGCG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          3707..3735
                     /label="EKo542.3"
                     /note="sequence: TGTATTAATGGTGAATACATGAGTGATCG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3717..3739
                     /label="XZp018test"
                     /note="sequence: GTGAATACATGAGTGATCGTGGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3890..3916
                     /label="XZp018.1"
                     /note="sequence: GAGAATGTCCAGTTTCATCTGTACATC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(3890..3926)
                     /label="oLSM0113"
                     /note="sequence: GAGAGGTGCTGATGTACAGATGAAACTGGACATTCTC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(3908..3928)
                     /label="XZp004.3"
                     /note="sequence: GGGAGAGGTGCTGATGTACAG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4040..4042
                     /label="E488Q"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(4059..4087)
                     /label="XZp018.2"
                     /note="sequence: GTCCCACGCTTGGATGCTCGCATTGGAGC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          4073..4094
                     /label="XZp018.3"
                     /note="sequence: ATCCAAGCGTGGGACGGGGTGC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4079..4079
                     /label="*"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4079..4081
                     /label="T501A"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(4166..4186)
                     /label="XZp030.1"
                     /note="sequence: GAAAATGCTGAGCAGGGATCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(4175..4194)
                     /label="XZp018.4"
                     /note="sequence: GGCTCCACGAAAATGCTGAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4682..4684
                     /label="Translation 4682-4684"
                     /translation="*"
     primer          complement(4685..4721)
                     /label="oLSM0065"
                     /note="sequence: ctgagatgagtttttgttcgggccctcctcgagcggc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4703..2142
                     /label="Digst. XZ024 (ApaI-BspTI)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4703..4735
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    4735..4737
                     /label="New Stop"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     CDS             4748..4765
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             4748..4765
                     /label="Translation 4748-4765"
                     /translation="HHHHHH"
     misc_feature    4766..4768
                     /label="cya"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(4769..4787)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     polyA_signal    4787..5001
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(4788..4805)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4867..4886)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4978..4996
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(4981..4998)
                     /label="EKo326.3"
                     /note="sequence: ccagcatgcctgctattg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    5034..5057
                     /label="BGH pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          5058..5076
                     /label="EKo293.3"
                     /note="sequence: cttctgaggcggaaagaac"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(5136..5151)
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          5268..5286
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5340..5387
                     /label="FRT"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_fwdcolor="#0080ff"
     primer          complement(5361..5388)
                     /label="EKo299.3"
                     /note="sequence: ggaagttcctatactttctagagaatag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     gene            5395..6415
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          5623..5644
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          6343..6361
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_signal    6547..6677
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(6560..6585)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6584..6603)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6717..6733)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6731..6750)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          7009..7028
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      7060..26
                     /label="pUC ori"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(7180..7199)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgtacgg
     1381 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     1441 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1501 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1561 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1621 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1681 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1741 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1801 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1861 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1921 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1981 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta
     2041 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2101 cgggaccgat ccagcctccg gactctagcg tttaaactta agcttcgcca ccatggggaa
     2161 gaaacccggg accagggtct tcaagaagtc gagccctaac tgcaagctca ccgtgtactt
     2221 gggcaagcgg gacttcgtag atcacctgga caaagtggac cctgtagatg gcgtggtgct
     2281 tgtggaccct gactacctga aggaccgcaa agtgtttgtg accctcacct gcgccttccg
     2341 ctatggccgt gaagacctgg atgtgctggg cttgtccttc cgcaaagacc tgttcatcgc
     2401 cacctaccag gccttccccc cggtgcccaa cccaccccgg ccccccaccc gcctgcagga
     2461 ccggctgctg aggaagctgg gccagcatgc ccaccccttc ttcttcacca taccccagaa
     2521 tcttccatgc tccgtcacac tgcagccagg cccagaggat acaggaaagg cctgcggcgt
     2581 agactttgag attcgagcct tctgtgctaa atcactagaa gagaaaagcc acaaaaggaa
     2641 ctctgtgcgg ctggtgatcc gaaaggtgca gttcgccccg gagaaacccg gcccccagcc
     2701 ttcagccgaa accacacgcc acttcctcat gtctgaccgg tccctgcacc tcgaggcttc
     2761 cctggacaag gagctgtact accatgggga gcccctcaat gtaaatgtcc acgtcaccaa
     2821 caactccacc aagaccgtca agaagatcaa agtctctgtg agacagtacg ccgacatctg
     2881 cctcttcagc accgcccagt acaagtgtcc tgtggctcaa ctcgaacaag atgaccaggt
     2941 atctcccagc tccacattct gtaaggtgta caccataacc ccactgctca gcgacaaccg
     3001 ggagaagcgg ggtctcgccc tggatgggaa actcaagcac gaggacacca acctggcttc
     3061 cagcaccatc gtgaaggagg gtgccaacaa ggaggtgctg ggaatcctgg tgtcctacag
     3121 ggtcaaggtg aagctggtgg tgtctcgagg cggggatgtc tctgtggagc tgccttttgt
     3181 tcttatgcac cccaagcccc acgaccacat ccccctcccc agaccccagt cagccgctcc
     3241 ggagacagat gtccctgtgg acaccaacct cattgaattt gataccaact atgccacaga
     3301 tgatgacatt gtgtttgagg actttgcccg gcttcggctg aaggggatga aggatgacga
     3361 ctatgatgat caactctgcG GTACCTCAGG CGGTTCCGGC AGCGGGGCGG GTAGCGGGTC
     3421 CAGTGGGTCA GAAACACCCG GCACGAGTGA GTCAGCAACG CCAGAGAGCG CTTTGCACTT
     3481 GGATCAAACG CCATCTCGCC AGCCTATTCC CAGTGAGGGT CTTCAGCTGC ATTTACCGCA
     3541 GGTTTTAGCT GACGCTGTCT CACGCCTGGT CCTGGGTAAG TTTGGTGACC TGACCGACAA
     3601 CTTCTCCTCC CCTCACGCTC GCAGAAAAGT GCTGGCTGGA GTCGTCATGA CAACAGGCAC
     3661 AGATGTTAAA GATGCCAAGG TGATAAGTGT TTCTACAGGA ACAAAATGTA TTAATGGTGA
     3721 ATACATGAGT GATCGTGGCC TTGCATTAAA TGACTGCCAT GCAGAAATAA TATCTCGGAG
     3781 ATCCTTGCTC AGATTTCTTT ATACACAACT TGAGCTTTAC TTAAATAACA AAGATGATCA
     3841 AAAAAGATCC ATCTTTCAGA AATCAGAGCG AGGGGGGTTT AGGCTGAAGG AGAATGTCCA
     3901 GTTTCATCTG TACATCAGCA CCTCTCCCTG TGGAGATGCC AGAATCTTCT CACCACATGA
     3961 GCCAATCCTG GAAGAACCAG CAGATAGACA CCCAAATCGT AAAGCAAGAG GACAGCTACG
     4021 GACCAAAATA GAGTCTGGTC AGGGGACGAT TCCAGTGCGC TCCAATGCGA GCATCCAAGC
     4081 GTGGGACGGG GTGCTGCAAG GGGAGCGGCT GCTCACCATG TCCTGCAGTG ACAAGATTGC
     4141 ACGCTGGAAC GTGGTGGGCA TCCAGGGATC CCTGCTCAGC ATTTTCGTGG AGCCCATTTA
     4201 CTTCTCGAGC ATCATCCTGG GCAGCCTTTA CCACGGGGAC CACCTTTCCA GGGCCATGTA
     4261 CCAGCGGATC TCCAACATAG AGGACCTGCC ACCTCTCTAC ACCCTCAACA AGCCTTTGCT
     4321 CAGTGGCATC AGCAATGCAG AAGCACGGCA GCCAGGGAAG GCCCCCAACT TCAGTGTCAA
     4381 CTGGACGGTA GGCGACTCCG CTATTGAGGT CATCAACGCC ACGACTGGGA AGGATGAGCT
     4441 GGGCCGCGCG TCCCGCCTGT GTAAGCACGC GTTGTACTGT CGCTGGATGC GTGTGCACGG
     4501 CAAGGTTCCC TCCCACTTAC TACGCTCCAA GATTACCAAA CCCAACGTGT ACCATGAGTC
     4561 CAAGCTGGCG GCAAAGGAGT ACCAGGCCGC CAAGGCGCGT CTGTTCACAG CCTTCATCAA
     4621 GGCGGGGCTG GGGGCCTGGG TGGAGAAGCC CACCGAGCAG GACCAGTTCT CACTCACGCC
     4681 Ctgagccgct cgaggagggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc
     4741 cgtcgaccat catcatcatc atcattgagt ttaaacccgc tgatcagcct cgactgtgcc
     4801 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4861 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4921 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4981 caatagcagg catgctgggg atgcggtggg ctctatggag atctatcgat gttctgggga
     5041 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5101 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5161 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5221 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg tccctttagg gttccgattt
     5281 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     5341 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     5401 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     5461 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     5521 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     5581 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     5641 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     5701 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     5761 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     5821 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     5881 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     5941 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     6001 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     6061 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     6121 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     6181 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     6241 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     6301 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     6361 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     6421 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     6481 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     6541 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6601 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6661 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6721 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6781 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6841 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6901 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7681 attacgcgca gaaaaaaagg atctcaa
//