LOCUS       pMT002-pcas-tyr-reins2x 9873 bp ds-DNA     circular     02-APR-2025
DEFINITION  .
KEYWORDS    "marker:NatR, gRNA \"R, AmpR, BB1000. AmpR-AgTEF1t R, BB1007. RNR2p
            seq1 R, GC2949. Cate-gRNA_R, BB1006. AmpR seq1 R, BB871. MoCl
            vector R, BB1080. gRNA MoCl BsaI GFP R, LB34. gRNA_scaffold_R,
            BB1004. intCas9 R, BB1002. RNR2p-AmpR R, BB1008. RNR2p seq2 R, 546.
            pUG6 Kan R, BB1009. CAS9 seq1 R"
FEATURES             Location/Qualifiers
     misc_feature    1..452
                     /label="panARS(opt)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     source          1..452
                     /label="source"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: source"
     primer          complement(57..74)
                     /label="MT082.panARS.Rev"
                     /note="sequence: aagtgcaaagagctcacc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    453..584
                     /label="tRNA-Tyr"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: tRNA-Tyr"
     misc_feature    453..654
                     /label="gRNA ""L"" tyrosine"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: gRNA ""L"" tyrosine"
     misc_feature    585..654
                     /label="3' HDV"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: 3' HDV"
     primer_bind     605..683
                     /label="BB1079. gRNA MoCl BsaI GFP F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    654..655
                     /label="removed TT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="label: removed TT"
     misc_feature    658..661
                     /label="61.5"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 61.5"
     misc_feature    662..683
                     /label="60.3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 60.3"
     misc_feature    662..1673
                     /label="GFP dropout cassette"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: GFP dropout cassette"
     RBS             810..821
                     /label="strong bacterial ribosome binding site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
     CDS             828..1541
                     /label="superfolder GFP"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: superfolder GFP"
     primer_bind     1031..1050
                     /label="BB776. GFPdrop int seq F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      1553..1624
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: rrnB T1 terminator"
     primer          complement(1633..1650)
                     /label="JJ033"
                     /note="sequence: gaaggtgagccagtgtga"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     terminator      1640..1667
                     /label="T7Te terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="label: T7Te terminator"
     misc_feature    1655..1673
                     /label="60.9"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 60.9"
     primer_bind     1655..1730
                     /label="BB1080. gRNA MoCl BsaI GFP R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1681..1760
                     /label="Scaffold"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="label: Scaffold"
     misc_feature    1681..1927
                     /label="gRNA ""R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: gRNA ""R"
     misc_feature    1761..1835
                     /label="Snr52t"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: Snr52t"
     misc_feature    1836..1844
                     /label="???"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: ???"
     primer_bind     1836..1860
                     /label="GC2946. pCAS_F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1845..1850
                     /label="BglII"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: BglII"
     primer          1855..1869
                     /label="MT362.Nat.F"
                     /note="sequence: agcttgcctcgtccc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     Promoter        1855..2233
                     /label="A. gossypii TEF1p promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: A. gossypii TEF1p"
     primer          1892..1909
                     /label="MT494.KanMX.F"
                     /note="sequence: catggaggcccagaatac"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     1910..1927
                     /label="GC2949. Cate-gRNA_R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     1910..1928
                     /label="LB34. gRNA_scaffold_R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1910..1928)
                     /label="AK733-SEQ.USERXII-1.R"
                     /note="sequence: GTCAAGACTGTCAAGGAGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1910..1928)
                     /label="MT224.LB34.R"
                     /note="sequence: GTCAAGACTGTCAAGGAGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     2001..2023
                     /label="546. pUG6 Kan R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     2031..2048
                     /label="BB1005. AgTEF1p seq1F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2174..2233
                     /label="60bp"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 60bp"
     misc_feature    2210..2232
                     /label="62.2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 62.2"
     misc_feature    2232..2233
                     /label="BsmBI hom"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: BsmBI hom"
     misc_feature    2807..2808
                     /label="BsmBI hom"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: BsmBI hom"
     misc_feature    2807..2833
                     /label=60
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 60"
     misc_feature    2807..2856
                     /label="70bp"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 70bp"
     Terminator      2807..3071
                     /label="A. gossypii TEF1t terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: A. gossypii TEF1t"
     primer          complement(2990..3008)
                     /label="MT495.KanMX.R"
                     /note="sequence: agtatagcgaccagcattc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2990..3009)
                     /label="MT363.Nat.R"
                     /note="sequence: cagtatagcgaccagcattc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     3038..3095
                     /label="BB1001. AgTEF1t-AmpR F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3038..3107
                     /label="70bp"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 70bp"
     misc_feature    3051..3071
                     /label="59.8"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: 59.8"
     primer_bind     3051..3107
                     /label="BB1000. AmpR-AgTEF1t R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3072..3095
                     /label="62.2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 62.2"
     misc_feature    complement(3072..4121)
                     /label="BB_AmpR cassette"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: BB_AmpR cassette"
     CDS             complement(3160..4020)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: AmpR"
     primer_bind     3249..3266
                     /label="BB871. MoCl vector R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     3792..3811
                     /label="BB1006. AmpR seq1 R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          3937..3953
                     /label="MT388.Amp.F"
                     /note="sequence: cagcgtttctgggtgag"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind     4081..4146
                     /label="BB1003. AmpR-RNR2p F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4081..4160
                     /label="80bp"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 80bp"
     misc_feature    4099..4121
                     /label="57.9"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 57.9"
     primer_bind     4099..4160
                     /label="BB1002. RNR2p-AmpR R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4122..4127
                     /label="SalI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: SalI"
     misc_feature    4128..4146
                     /label="61.5"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 61.5"
     misc_feature    4128..4827
                     /label="RNR2p"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: RNR2p"
     primer_bind     4272..4291
                     /label="BB1007. RNR2p seq1 R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     4765..4784
                     /label="BB1008. RNR2p seq2 R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4828..9003
                     /label="Cas9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: Cas9"
     misc_feature    4998..5015
                     /label="66.6"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 66.6"
     primer_bind     4998..5076
                     /label="BB1004. intCas9 R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    5016..5016
                     /label="Pt Mut to remove BsmBI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: Pt Mut to remove BsmBI"
     misc_feature    5017..5076
                     /label="60bp"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: 60bp"
     primer_bind     5145..5164
                     /label="BB1009. CAS9 seq1 R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    8941..8961
                     /label="NLS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: NLS"
     misc_feature    8978..9000
                     /label="8xHIS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="label: 8xHIS"
     misc_feature    9004..9193
                     /label="CYC1t - Cate lab"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: CYC1t - Cate lab"
     misc_feature    9194..9199
                     /label="SacII"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: SacII"
     misc_feature    9200..9867
                     /label="ColEI origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="label: ColEI origin"
     primer_bind     9303..9320
                     /label="BB774. MoCloEnt chk F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          9445..9462
                     /label="MT081.ColEI.For"
                     /note="sequence: TTCTCATAGCTCACGCTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(9848..9873)
                     /label="MT080.BBpCas-2micron.Rev"
                     /note="sequence: ACTAGTGTAGAAAAGATCAAAGGATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    9868..9873
                     /label="SpeI"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: SpeI"
ORIGIN
        1 tcaacatctt tggataatat cagaatgaga aagaacagat acgcagtacg ttttttggtg
       61 agctctttgc acttctttag ttctttccat caatatcagt tttttaaact tttaggacta
      121 aaagtgatgt ttaacttcaa aatgtttaaa attttgttct tcccgacgtt cattaagaat
      181 actaatacac tttaataatt attttaatat tttgttctaa ataatgactt ttaattaaaa
      241 aagataaaat ataaaaacat cataataact caccagaggt taagaacaaa aaaacaaatt
      301 agatatctgc taatccaata tagttaaatc aatctttcct tggtataatg ggtatattac
      361 atatatttca aggaccgaca ctcctaccaa atatctaaaa tttaccatat taacataaca
      421 tgtatataaa cgtcaaatca taatcagcac taGTATATGT GTTATGTAGT ATACTCTTTC
      481 TTCAACAATT AAATACTCTC GGTAGCCAAG TTGGTTTAAG GCGCAAGACT GTAATTTATC
      541 ACTACGAAAT CTTGAGATCG GGCGTTCGAC TCGCCCCCGG GAGAGATGGC CGGCATGGTC
      601 CCAGCCTCCT CGCTGGCGCC GGCTGGGCAA CACCTTCGGG TGGCGAATGG GACTCGAGAC
      661 Cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat
      721 gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa
      781 acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg
      841 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      901 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      961 agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga
     1021 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1081 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1141 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1201 tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt
     1261 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1321 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1381 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1441 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1501 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc atcaaataaa
     1561 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1621 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataGGTCTCC
     1681 GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT
     1741 GGCACCGAGT CGGTGCTTTT TTTATTTTTT GTCACTATTG TTATGTAAAA TGCCACCTCT
     1801 GACAGTATGG AACGCAAACT TCTGTCTAGT GGATATAGGT CTAGAGATCT GTTTAGCTTG
     1861 CCTCGTCCCC GCCGGGTCAC CCGGCCAGCG ACATGGAGGC CCAGAATACC CTCCTTGACA
     1921 GTCTTGACGT GCGCAGCTCA GGGGCATGAT GTGACTGTCG CCCGTACATT TAGCCCATAC
     1981 ATCCCCATGT ATAATCATTT GCATCCATAC ATTTTGATGG CCGCACGGCG CGAAGCAAAA
     2041 ATTACGGCTC CTCGCTGCAG ACCTGCGAGC AGGGAAACGC TCCCCTCACA GACGCGTTGA
     2101 ATTGTCCCCA CGCCGCGCCC CTGTAGAGAA ATATAAAAGG TTAGGATTTG CCACTGAGGT
     2161 TCTTCTTTCA TATACTTCCT TTTAAAATCT TGCTAGGATA CAGTTCTCAC ATCACATCCG
     2221 AACATAAACA ACaatgggta ctaccttaga tgatacagcc tacagataca gaacatcagt
     2281 ccctggtgat gctgaagcaa ttgaggcttt agacggttca ttcaccaccg acaccgtctt
     2341 tagagtaacc gccaccggtg atggatttac cttaagagaa gtcccagtcg accctccatt
     2401 aactaaagtc tttccagatg atgaatctga tgacgaaagc gacgacggag aagatggtga
     2461 cccagattca agaactttcg tagcatacgg tgatgacggt gatttggctg gttttgtagt
     2521 cgtttcttat tcaggttgga atagaaggtt gaccgttgaa gatatagaag tcgccccaga
     2581 gcatagaggt catggtgtag gaagagcttt gatgggtttg gctacagaat ttgcaagaga
     2641 gagaggagcc ggtcatttat ggttagaagt tactaatgtt aacgcccctg ctatccatgc
     2701 ttatagaaga atgggtttca cattatgtgg tttagatact gctttatatg atggaacagc
     2761 atctgacggt gaacaggcct tgtatatgtc tatgccttgc ccttaaTCAG TACTGACAAT
     2821 AAAAAGATTC TTGTTTTCAA GAACTTGTCA TTTGTATAGT TTTTTTATAT TGTAGTTGTT
     2881 CTATTTTAAT CAAATGTTAG CGTGATTTAT ATTTTTTTTC GCCTCGACAT CATCTGCCCA
     2941 GATGCGAAGT TAAGTGCGCA GAAAGTAATA TCATGCGTCA ATCGTATGTG AATGCTGGTC
     3001 GCTATACTGC TGTCGATTCG ATACTAACGC CGCCATCCAG TGTCGAAAAC GAGCTCTCGA
     3061 GAACCCTTAA Tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3121 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3181 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3241 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3301 ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3361 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3421 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3481 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3541 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3601 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3661 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3721 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3781 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3841 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3901 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3961 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4081 catatttgaa tgtatttaga aaaataaaca aataggggtt cGTCGACAGT CGAACAAGAA
     4141 GCAGGCAAAG TTTAGAGCAC TGCCCCTCCG CACTCAAAAA AGAAAAAACT AGGAGGAAAA
     4201 TAAAATTCTC AACCACACAA ACACATAAAC ACATACAAAT ACAAATACAA GCTTATTTAC
     4261 TTGACATCGC GCGATCTTCC ACTATTCAGC GCCGTCCGCC CTCTCTCGTG TTTTTTGTTT
     4321 ACGCGACAAC TATGCGAAAT CCGGAGCAAC GGGCAACCGT TTGGGGAAAG ACCACACCCA
     4381 CGCGCGATCG CCATGGCAAC GAGGTCGCAC ACGCCCCACA CCCAGACCTC CCTGCGAGCG
     4441 GGCATGGGTA CAATGTCCCC GTTGCCACAG ACACCACTTC GTAGCACAGC GCAGAGCGTA
     4501 GCGTGTTGTT GCTGCTGACA AAAGAAAATT TTTCTTAGCA AAGCAAAGGA GGGGAAGCAC
     4561 GGGCAGATAG CACCGTACCA TACCCTTGGA AACTCGAAAT GAACGAAGCA GGAAATGAGA
     4621 GAATGAGAGT TTTGTAGGTA TATATAGCGG TAGTGTTTGC GCGTTACCAT CATCTTCTGG
     4681 ATCTATCTAT TGTTCTTTTC CTCATCACTT TCCCCTTTTT CGCTCTTCTT CTTGTCTTTT
     4741 ATTTCTTTCT TTTTTTTAAT TGTTCCCTCG ATTGGCTATC TACCAAAGAA TCCAAACTTA
     4801 ATACACGTAT TTATTTGTCC AATTACCATG GATAAGAAAT ACTCAATAGG CTTAGATATC
     4861 GGCACAAATA GCGTCGGATG GGCGGTGATC ACTGATGAAT ATAAGGTTCC GTCTAAAAAG
     4921 TTCAAGGTTC TGGGAAATAC AGACCGCCAC AGTATCAAAA AAAATCTTAT AGGGGCTCTT
     4981 TTATTTGACA GTGGAGAGAC AGCGGAAGCG ACTCGACTCA AACGGACAGC TCGTAGAAGG
     5041 TATACACGTC GGAAGAATCG TATTTGTTAT CTACAGGAGA TTTTTTCAAA TGAGATGGCG
     5101 AAAGTAGATG ATAGTTTCTT TCATCGACTT GAAGAGTCTT TTTTGGTGGA AGAAGACAAG
     5161 AAGCATGAAC GTCATCCTAT TTTTGGAAAT ATAGTAGATG AAGTTGCTTA TCATGAGAAA
     5221 TATCCAACTA TCTATCATCT GCGAAAAAAA TTGGTAGATT CTACTGATAA AGCGGATTTG
     5281 CGCTTAATCT ATTTGGCCTT AGCGCATATG ATTAAGTTTC GTGGTCATTT TTTGATTGAG
     5341 GGAGATTTAA ATCCTGATAA TAGTGATGTG GACAAACTAT TTATCCAGTT GGTACAAACC
     5401 TACAATCAAT TATTTGAAGA AAACCCTATT AACGCAAGTG GAGTAGATGC TAAAGCGATT
     5461 CTTTCTGCAC GATTGAGTAA ATCAAGACGA TTAGAAAATC TCATTGCTCA GCTCCCCGGT
     5521 GAGAAGAAAA ATGGCTTATT TGGGAATCTC ATTGCTTTGT CATTGGGTTT GACCCCTAAT
     5581 TTTAAATCAA ATTTTGATTT GGCAGAAGAT GCTAAATTAC AGCTTTCAAA AGATACTTAC
     5641 GATGATGATT TAGATAATTT ATTGGCGCAA ATTGGAGATC AATATGCTGA TTTGTTTTTG
     5701 GCAGCTAAGA ATTTATCAGA TGCTATTTTA CTTTCAGATA TCCTAAGAGT AAATACTGAA
     5761 ATAACTAAGG CTCCCCTATC AGCTTCAATG ATTAAACGCT ACGATGAACA TCATCAAGAC
     5821 TTGACTCTTT TAAAAGCTTT AGTTCGACAA CAACTTCCAG AAAAGTATAA AGAAATCTTT
     5881 TTTGATCAAT CAAAAAACGG ATATGCAGGT TATATTGATG GGGGAGCTAG CCAAGAAGAA
     5941 TTTTATAAAT TTATCAAACC AATTTTAGAA AAAATGGATG GTACTGAGGA ATTATTGGTG
     6001 AAACTAAATC GTGAAGATTT GCTGCGCAAG CAACGGACCT TTGACAACGG CTCTATTCCC
     6061 CATCAAATTC ACTTGGGTGA GCTGCATGCT ATTTTGAGAA GACAAGAAGA CTTTTATCCA
     6121 TTTTTAAAAG ACAATCGTGA GAAGATTGAA AAAATCTTGA CTTTTCGAAT TCCTTATTAT
     6181 GTTGGTCCAT TGGCGCGTGG CAATAGTCGT TTTGCATGGA TGACTCGGAA GTCTGAAGAA
     6241 ACAATTACCC CATGGAATTT TGAAGAAGTT GTCGATAAAG GTGCTTCAGC TCAATCATTT
     6301 ATTGAACGCA TGACAAACTT TGATAAAAAT CTTCCAAATG AAAAAGTACT ACCAAAACAT
     6361 AGTTTGCTTT ATGAGTATTT TACGGTTTAT AACGAATTGA CAAAGGTCAA ATATGTTACT
     6421 GAAGGAATGC GAAAACCAGC ATTTCTTTCA GGTGAACAGA AGAAAGCCAT TGTTGATTTA
     6481 CTCTTCAAAA CAAATCGAAA AGTAACCGTT AAGCAATTAA AAGAAGATTA TTTCAAAAAA
     6541 ATAGAATGTT TTGATAGTGT TGAAATTTCA GGAGTTGAAG ATAGATTTAA TGCTTCATTA
     6601 GGTACCTACC ATGATTTGCT AAAAATTATT AAAGATAAAG ATTTTTTGGA TAATGAAGAA
     6661 AATGAAGATA TCTTAGAGGA TATTGTTTTA ACATTGACCT TATTTGAAGA TAGGGAGATG
     6721 ATTGAGGAAA GACTTAAAAC ATATGCTCAC CTCTTTGATG ATAAGGTGAT GAAACAGCTT
     6781 AAACGTCGCC GTTATACTGG TTGGGGACGT TTGTCTCGAA AATTGATTAA TGGTATTAGG
     6841 GATAAGCAAT CTGGCAAAAC AATATTAGAT TTTTTGAAAT CAGATGGTTT TGCCAATCGC
     6901 AATTTTATGC AGCTGATCCA TGATGATAGT TTGACATTTA AAGAAGACAT TCAAAAAGCA
     6961 CAAGTGTCTG GACAAGGCGA TAGTTTACAT GAACATATTG CAAATTTAGC TGGTAGCCCT
     7021 GCTATTAAAA AAGGTATTTT ACAGACTGTA AAAGTTGTTG ATGAATTGGT CAAAGTAATG
     7081 GGGCGGCATA AGCCAGAAAA TATCGTTATT GAAATGGCAC GTGAAAATCA GACAACTCAA
     7141 AAGGGCCAGA AAAATTCGCG AGAGCGTATG AAACGAATCG AAGAAGGTAT CAAAGAATTA
     7201 GGAAGTCAGA TTCTTAAAGA GCATCCTGTT GAAAATACTC AATTGCAAAA TGAAAAGCTC
     7261 TATCTCTATT ATCTCCAAAA TGGAAGAGAC ATGTATGTGG ACCAAGAATT AGATATTAAT
     7321 CGTTTAAGTG ATTATGATGT CGATCACATT GTTCCACAAA GTTTCCTTAA AGACGATTCA
     7381 ATAGACAATA AGGTCTTAAC GCGTTCTGAT AAAAATCGTG GTAAATCGGA TAACGTTCCA
     7441 AGTGAAGAAG TAGTCAAAAA GATGAAAAAC TATTGGAGAC AACTTCTAAA CGCCAAGTTA
     7501 ATCACTCAAC GTAAGTTTGA TAATTTAACG AAAGCTGAAC GTGGAGGTTT GAGTGAACTT
     7561 GATAAAGCTG GTTTTATCAA ACGCCAATTG GTTGAAACTC GCCAAATCAC TAAGCATGTG
     7621 GCACAAATTT TGGATAGTCG CATGAATACT AAATACGATG AAAATGATAA ACTTATTCGA
     7681 GAGGTTAAAG TGATTACCTT AAAATCTAAA TTAGTTTCTG ACTTCCGAAA AGATTTCCAA
     7741 TTCTATAAAG TACGTGAGAT TAACAATTAC CATCATGCCC ATGATGCGTA TCTAAATGCC
     7801 GTCGTTGGAA CTGCTTTGAT TAAGAAATAT CCAAAACTTG AATCGGAGTT TGTCTATGGT
     7861 GATTATAAAG TTTATGATGT TCGTAAAATG ATTGCTAAGT CTGAGCAAGA AATAGGCAAA
     7921 GCAACCGCAA AATATTTCTT TTACTCTAAT ATCATGAACT TCTTCAAAAC AGAAATTACA
     7981 CTTGCAAATG GAGAGATTCG CAAACGCCCT CTAATCGAAA CTAATGGGGA AACTGGAGAA
     8041 ATTGTCTGGG ATAAAGGGCG AGATTTTGCC ACAGTGCGCA AAGTATTGTC CATGCCCCAA
     8101 GTCAATATTG TCAAGAAAAC AGAAGTACAG ACAGGCGGAT TCTCCAAGGA GTCAATTTTA
     8161 CCAAAAAGAA ATTCGGACAA GCTTATTGCT CGTAAAAAAG ACTGGGATCC AAAAAAATAT
     8221 GGTGGTTTTG ATAGTCCAAC GGTAGCTTAT TCAGTCCTAG TGGTTGCTAA GGTGGAAAAA
     8281 GGGAAATCGA AGAAGTTAAA ATCCGTTAAA GAGTTACTAG GGATCACAAT TATGGAAAGA
     8341 AGTTCCTTTG AAAAAAATCC GATTGACTTT TTAGAAGCTA AAGGATATAA GGAAGTTAAA
     8401 AAAGACTTAA TCATTAAACT ACCTAAATAT AGTCTTTTTG AGTTAGAAAA CGGTCGTAAA
     8461 CGGATGCTGG CTAGTGCCGG AGAATTACAA AAAGGAAATG AGCTGGCTCT GCCAAGCAAA
     8521 TATGTGAATT TTTTATATTT AGCTAGTCAT TATGAAAAGT TGAAGGGTAG TCCAGAAGAT
     8581 AACGAACAAA AACAATTGTT TGTGGAGCAG CATAAGCATT ATTTAGATGA GATTATTGAG
     8641 CAAATCAGTG AATTTTCTAA GCGTGTTATT TTAGCAGATG CCAATTTAGA TAAAGTTCTT
     8701 AGTGCATATA ACAAACATAG AGACAAACCA ATACGTGAAC AAGCAGAAAA TATTATTCAT
     8761 TTATTTACGT TGACGAATCT TGGAGCTCCC GCTGCTTTTA AATATTTTGA TACAACAATT
     8821 GATCGTAAAC GATATACGTC TACAAAAGAA GTTTTAGATG CCACTCTTAT CCATCAATCC
     8881 ATCACTGGTC TTTATGAAAC ACGCATTGAT TTGAGTCAGC TAGGAGGTGA CGGTGGAGGG
     8941 CCAAAAAAGA AAAGAAAAGT TGAAGATGCT TCTGCTCATC ATCACCATCA CCATCACCAT
     9001 TAAATCCGCT CTAACCGAAA AGGAAGGAGT TAGACAACCT GAAGTCTAGG TCCCTATTTA
     9061 TTTTTTTATA GTTATGTTAG TATTAAGAAC GTTATTTATA TTTCAAATTT TTCTTTTTTT
     9121 TCTGTACAGA CGCGTGTACG CATGTAACAT TATACTGAAA ACCTTGCTTG AGAAGGTTTT
     9181 GGGACGCTCG AAGCCGCGGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
     9241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     9301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     9361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     9421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     9481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     9541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     9601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     9661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
     9721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     9781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     9841 TCAAGAAGAT CCTTTGATCT TTTCTACACT AGT
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