LOCUS       pAK016_(level0_TEF3-tet 2326 bp ds-DNA     circular     16-APR-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      7..41
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)"
     misc_feature    112..771
                     /label="CmR"
     primer_bind     686..703
                     /label="CAT-R"
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      953..1541
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="RIGHT"
     primer_bind     1442..1461
                     /label="pBR322ori-F"
                     /note="pBR322 origin, forward primer"
     source          1542..1642
                     /label="source"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1655..2212
                     /label="TEF3p"
     misc_feature    2195..2202
                     /label="TATAWAWR box"
     misc_feature    2253..2315
                     /label="TEF3p"
ORIGIN
        1 agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
       61 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
      121 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      181 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      241 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      301 atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa
      361 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      421 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
      481 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      541 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      601 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      661 aacggtctgg ttataggtac attgagcaac tgactgaaat gccTCAAAAT GTTctttacg
      721 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      781 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      841 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
      901 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      961 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1021 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1081 cgcagatAcc aaatactgtt cttctagtgt agccgtagtt aggccacTTc ttcaagaact
     1141 ctgtagcacc gcctaAatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1201 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1261 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1321 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1381 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1441 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1501 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1561 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1621 ctgattctgt ggataaccgt agtcggTCTC AAACGAAGCA AAGAAGTAAC AGCAGGAAAG
     1681 AAAAACAAAC ACAACAAAAA AAAACAAGCA GCATAGCATC AACAGAAATT TCTAAAGAGA
     1741 ACCAAATTCA CCCCAGAAAC AACCGCACAA ATACGACATC CATCCACCTT TCTTTTATCT
     1801 TCTTTTTCTG ATCTGATAAT TAGTTTCATA TACAATACGT AGAAACAGGC GCACAGCACC
     1861 CAGACCTGGC TTCTGCCCCA GTGTATAAGC AATGTAGCAT AATTGGAAAA AAAAACGAAA
     1921 AATACCGAAA ATAAGTGGGA AGCTGGGCCA CAGGAGTGGG GCGGGATGCG ACTGGTTCTG
     1981 AGCGGGACCG GGTAATAAGG TTGAAAAACT TTGAATTGAT GGAATAAGTA ACTTCTTTCT
     2041 TTTCGCTGGC GGGAGAAGGA AAAAAAAAAA TTTTTTTTCC TTCTGTTTAG TACTGGGACA
     2101 TTGAGAAGGC GTGTCAATTT TGAATAATTA GAGTGGTCAA AAAAATTTTT TTTGCTTGGG
     2161 ATACCCTTTT TCGATAATGT AAATTTTTTT TGAATATAAA AGGAGATTGA AATCCCTATC
     2221 AGTGATAGAG ATATCCCTAT CAGTGATAGA GATTGATTTG TAGTTTATTT ACTAGTATTC
     2281 TCATAGTTCT AACTCCAAGA GAAGTAACAT TAAAGTATGT GAGACC
//