LOCUS       pN4                     5154 bp ds-DNA     circular     02-OCT-2024
DEFINITION  pY3-NbRec-Galv2.
ACCESSION   pY3-NbRec-Galv2
VERSION     pY3-NbRec-Galv2
COMMENT     Imported using the Genbank importer. File name:
            FSX5NF_1_pY3-NbRec-Galv2.gbk Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1002..1666
                     /label="GAL1,10 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1677..1695
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1703..1762
                     /label="AGA2 (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="90.0"
                     /match_length="23.0"
                     /fragment="True"
                     /other="CDS"
                     /translation="MQLLRCFSIFSVIASVLARD"
     misc_feature    1751..1770
                     /label="FWD Annealing"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2137..2156
                     /label="REV Annealing"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(2167..2216)
                     /label="bom (fragment)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2266..2937)
                     /label="TRP1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRKRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNAKK"
     promoter        complement(2938..3218)
                     /label="TRP1 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3219..3268)
                     /label="bom (fragment)"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3370..3405)
                     /label="rop (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
                     /translation="LARFGDDGENL*"
     misc_feature    3473..3976
                     /label="CEN/ARS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(4087..4947)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4948..5052)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtgc ggccgcccct gaattcagct tttgattaag
      721 ccttctagtc caaaaaacac gtttttttgt catttatttc attttcttag aatagtttag
      781 tttattcatt ttatagtcac gaatgtttta tgattctata tagggttgca aacaagcatt
      841 tttcatttta tgttaaaaca atttcaggtt taccttttat tctgcttgtg gtgacgcgtg
      901 tatccgcccg ctcttttggt cacccatgta tagaccatgg tacgcggcac cgacttatac
      961 cgatgatatt agtcagactt tagtgagggt tgaattcgaa ttttcaaaaa ttcttacttt
     1021 ttttttggat ggacgcaaag aagtttaata atcatattac atggcattac caccatatac
     1081 atatccatat acatatccat atctaatctt acttatatgt tgtggaaatg taaagagccc
     1141 cattatctta gcctaaaaaa accttctctt tggaactttc agtaatacgc ttaactgctc
     1201 attgctatat tgaagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag
     1261 actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg
     1321 cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg
     1381 aaaaattggc agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca
     1441 taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg
     1501 atgatttttg atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa
     1561 tactttcaac attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa
     1621 aaaattgtta atatacctct atactttaac gtcaaggaga aaaaaccccg gatccgtaat
     1681 acgactcact ataggggcta gcatgcagtt acttcgctgt ttttcaatat tttctgttat
     1741 tgcttcagtt ttagcaagag accctgcagc ttaagactaa gcatgcatgg tctcataagc
     1801 ttggtaccgc ggctagtaat agctcgagat ctgataacaa cagtgtagat gtaacaaaat
     1861 cgactttgtt cccactgtac ttttagctcg tacaaaatac aatatacttt tcatttctcc
     1921 gtaaacaaca tgttttccca tgtaatatcc ttttctattt ttcgttccgt taccaacttt
     1981 acacatactt tatatagcta ttcacttcta tacactaaaa aactaagaca attttaattt
     2041 tgctgcctgc catatttcaa tttgttataa attcctataa tttatcctat tagtagctaa
     2101 aaaaagatga atgtgaatcg aatcctaaga gaattgagct ccaattcact agtgcactgc
     2161 agtacacctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaggca
     2221 agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt
     2281 gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac
     2341 atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc
     2401 accagctaac ataaaatgta agctttcggg gctctcttgc cttccaaccc agtcagaaat
     2461 cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa
     2521 cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag
     2581 tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg
     2641 cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg
     2701 attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac
     2761 aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca
     2821 tataataccc agcaagtcag catcagaatc tagagcacat tctgcggcct ctgtgctctg
     2881 caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa cagacatact
     2941 ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc accaatgccc
     3001 tccctcttgg ccctctcctt ttcttttttc gaccgaatta attcttaatc ggcaaaaaaa
     3061 gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct atttttcaat aaagaatatc
     3121 ttccactact gccatctggc gtcataactg caaagtacac atatattacg atgctgtcta
     3181 ttaaatgctt cctatattat atatatagta atgtcgttta tggtgcactc tcagtacaat
     3241 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tgtccgggag
     3361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagtcgaa agggcctcgt
     3421 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga gtatcacgtg
     3481 ctataaaaat aattataatt taaatttttt aatataaata tataaattaa aaatagaaag
     3541 taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg
     3601 gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg
     3661 aattgtttca tcttgtctgt gtagaaaacc acacacgaaa atcctgtgat tttacatttt
     3721 acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata
     3781 tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc
     3841 cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa
     3901 ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag
     3961 ttacaggcaa gcgatccgtc ccgagcggcc gcgattatca aaaaggatct tcacctagat
     4021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4201 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc
     4261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5041 aggggttccg cgcacatttc cccgaaaagt gccacctgtc atgaccaaaa tcccttaacg
     5101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//