LOCUS       Exported               14058 bp ds-DNA     circular SYN 23-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14058)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14058
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    861..1367
                     /label=cLUC
     misc_feature    1377..4499
                     /label=Sr62NLRa
     terminator      4512..5153
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(5161..5177)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5380..5404
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6704..7333
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7767..8834
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8900..9094
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9438..9578
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9764..10352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10439..11233)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11658..11682
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11760..11934
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11974..12999)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13067..13744)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    13935..13956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13971..14001
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14009..14025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14033..14049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc atgtccggtt atgtaaacaa tccggaagcg accaacgcct
      901 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
      961 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     1021 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     1081 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     1141 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     1201 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     1261 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgg
     1321 gaggtggctc atctggcgga ggtcagatct cgtacgcgtc ccggggcggt accggcatgg
     1381 aggagatcgt gcttggactg tccaagacgg tggtggaggg gacgctggtc aaggtgaagg
     1441 cggcgattga cgaggagtcg aggctgaagc tcgcggtgca gagcgacctg gtgttcatca
     1501 cgggggagtt cgagatgatg cagtcgttcc tcaacgtcgc cgacgcggag cgcatcaaga
     1561 acaacgccgt caagacgtgg gtgaggcagc tccgcgacct ggcctacgac accgaggact
     1621 gcatcgagct ggtcgtccac ctggatccca agcccagatg gtggcgccgc ctgctggtcc
     1681 tgccttgcct gccggcggtg tcgctgccaa tggatgacgc ggcggcggag ataaaggagc
     1741 tcaagggcag ggtggagttc gtgagccagc ggaatatgcg ctacaacctc atcaccgact
     1801 tcggcggcgc caagtccgcc gcgcagcagc agcttgaccg ggccgccgga gctctcgaca
     1861 tcgccgtgga ggcggcggcg aggaaagggc gtagcttggt ggatctgacc gagttgatcc
     1921 ccaaaatgga ggaccgccct gagctcggcg tgatctcggt gtggggcaca ggcggcgacc
     1981 ttggggtggc gtccatcgtc aggaaggcct acgacgaccc tgaaatacgc aagaacttcc
     2041 atagtcgtgg ctgggccaag ttgacccacc ctttcgatcc gcggaagatc ctccggagct
     2101 tgctcattca gttctgcact aacactgctg ctccacagaa gcaaggggag actctggatg
     2161 tagattcgtt gctgaagatg gaaaagacag tggtggaaga aggggagctc gtcaagcagt
     2221 ttgtgagtca tgtgagcacc cacaggttcc tcgtcgtcct cgaaggcgtg tcaacgatgg
     2281 cggaatggga tgccctccgg atgtacctgc cagacatggg taatgggagt cagatcatcg
     2341 tatccacaca gcagttcgac atcgcgagct tgtgcaccgg gcaactgcac aaagtttcag
     2401 agttgagaaa attcacacct gaccactctg tttgtgtgtt cttcaaggag gtttctggag
     2461 gtgatgatcc tggaaatgca atgctggagg gagatgacaa agctgaactt attgggcgtg
     2521 acacagaaga ggaggagctt atggagcaga ttgatgaggt ttcccgtgtg atctcggtat
     2581 gggggattgc tggtgtagga aaatcggcac ttgtcagaag tgcctactgc aagtatctcc
     2641 aggaccgccc caactttgct gggttaaatc tgacctacat acacgggcgc gtgaccgtgc
     2701 cccatccctt taatctgagg gagttctgcc gcagcttgct cctgaacttg tcctctgagc
     2761 ctgctaaaac caaagaggac gccgtcgctg aactggcaaa catgaccaat ccaactgatg
     2821 tctgccgtaa gcttcttagc gacgatcaac aacagaagca gtgccttatt gttattgatg
     2881 acctccagtc cactgaagaa tgggacagaa taaaacaggc cttgtcattt ggtaaagaat
     2941 gttgtatcat agttgttacg aatgattcaa gtgttgccaa atattgttca gggcaagatc
     3001 agaaactcat gttcaacgtc aaaggtcttg gtgacgagca tgcccttgac ctcttcaaca
     3061 cggtatatga cggcatgcct aaccacatca cacgggatga ggacatgatc aatcagtcga
     3121 agctaatgtt aaacaaatgc ggcggactac ccaaggtgat agttgcaatt ggtcgtttct
     3181 tggccaagag tctgaattgg gaggccatga atactaactt tattcaccag ttggaaaaca
     3241 acccagagct ggctagtata catagcctat ttagttggct ggactcctac ttccacaact
     3301 gtcctgatga actcaagcca tgcatcttct atttgtcaat cttccctcga cgccacggca
     3361 ttcgtcggag gcgtctggtg aggcggtgga tcgccgaggg ctactcaagg gacaccgacg
     3421 ggaatctcgc ggaagtgaac ggggaaaatt acttctccag gcttgtcaac ctcagcatgc
     3481 tcacagatgc tgagcgtggt gtcaacgcgg ctgggacagg gaggaaaatg gccatgtgca
     3541 acatcaacga tttcttccgg gagtacattg tgtcgcggcg aatggaggag aaccatgtgt
     3601 ttgcactgga ggggaggtgc agccagacca cacggcgcac tgggcggcac ctggtcatca
     3661 accaaagctg ggacggagat gagaacgtgt tcaacaggat cgagttctcg cgactgcggt
     3721 cggtgacagt gttcggaccc tggaagccat tcctggcctc cgacaagatg agggtgctcc
     3781 gcgtgcttga tctggaaggc actgaaggtc tgacagacga cgacatcaag agcatcgtca
     3841 aacggctgcc tcgactgaag ttcctctccc tgcgaaaatg caaaaacatc tttcggctgc
     3901 cgaagtccct gggtcgcctg aggcagctcc agaccctgga tgtgaggcac actgccatag
     3961 cccagctgcc agcgaccgtc gtgaagctac agaagttgca gtatgttcgt gccggtaaca
     4021 caccagagga atatgccggc agcgacagca cggaatctag cacctcggtg cccgcctgtt
     4081 tctcatgtgg ccctgttggc ggtcacattg tcggcgttga ggtgcctcgc ggggtcgaga
     4141 atctgaccaa cctgcacaca ctgggtgtca tcaaagccac tgtggcggga ctcaaggagc
     4201 tcaaaaagct tacccagctg cgcaagctcg gtgtgtctgg catcaaccgg agaaaccaca
     4261 aagagctctg tgccgtcgtc tccggacatg gtcatctgga atccctgtcg atatggcttg
     4321 acaaggacac cgaggggatt gctgaccctt gttcgccgag caatgatgac ttcaagcctc
     4381 cagagaagct acggaggctc aaactccacg ggcacatcgg caatttgcca gcatgggtta
     4441 gcactctcac catgctggat ttggtggaag tatccaagtc tgctgctcaa gatccatgag
     4501 tcgacctgca gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc
     4561 atcagtttca ttgcgcacac accagaatcc tactgagttc gagtattatg gcattgggaa
     4621 acatgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt
     4681 cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt
     4741 tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga
     4801 aaatataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct
     4861 aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac
     4921 taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg
     4981 tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc
     5041 agattctaat cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa
     5101 aatatttttt aatgcatttt atgacttgcc aattgattga caacatgcat caagcttggc
     5161 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5221 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5281 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
     5341 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     5401 aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt
     5461 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     5521 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     5581 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     5641 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     5701 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     5761 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     5821 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     5881 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     5941 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     6001 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     6061 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     6121 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     6181 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     6241 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     6301 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     6361 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     6421 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     6481 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     6541 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     6601 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     6661 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     6721 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     6781 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     6841 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     6901 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     6961 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     7021 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     7081 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     7141 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     7201 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     7261 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     7321 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     7381 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     7441 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     7501 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     7561 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     7621 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     7681 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     7741 ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg
     7801 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     7861 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     7921 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     7981 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     8041 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     8101 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     8161 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     8221 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     8281 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     8341 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     8401 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     8461 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     8521 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     8581 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     8641 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     8701 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     8761 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     8821 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     8881 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     8941 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     9001 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
     9061 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
     9121 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
     9181 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
     9241 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
     9301 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     9361 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     9421 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     9481 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     9541 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     9601 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9661 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9721 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9781 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9841 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9901 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9961 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10021 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10081 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10141 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10201 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10261 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10381 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
    10441 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    10501 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    10561 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    10621 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    10681 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    10741 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    10801 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    10861 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    10921 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    10981 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    11041 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    11101 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    11161 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    11221 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    11281 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    11341 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    11401 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
    11461 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
    11521 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
    11581 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    11641 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    11701 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
    11761 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
    11821 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
    11881 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
    11941 cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg gacgagtgct
    12001 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
    12061 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
    12121 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
    12181 ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg
    12241 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
    12301 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
    12361 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
    12421 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
    12481 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
    12541 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
    12601 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg
    12661 ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
    12721 gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat
    12781 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
    12841 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
    12901 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
    12961 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc
    13021 tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
    13081 ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc
    13141 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
    13201 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    13261 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
    13321 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
    13381 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
    13441 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca
    13501 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
    13561 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
    13621 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
    13681 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
    13741 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    13801 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
    13861 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    13921 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    13981 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    14041 agctatgacc atgattac
//
