LOCUS       Exported               14052 bp ds-DNA     circular SYN 23-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14052
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    861..1367
                     /label=cLUC
     misc_feature    1377..4493
                     /label=Sr62NLRb
     terminator      4506..5147
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(5155..5171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5374..5398
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6698..7327
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7761..8828
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8894..9088
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9432..9572
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9758..10346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10433..11227)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11652..11676
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11754..11928
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11968..12993)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13061..13738)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    13929..13950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13965..13995
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14003..14019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14027..14043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc atgtccggtt atgtaaacaa tccggaagcg accaacgcct
      901 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
      961 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     1021 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     1081 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     1141 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     1201 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     1261 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgg
     1321 gaggtggctc atctggcgga ggtcagatct cgtacgcgtc ccggggcggt accggcatgg
     1381 aggagatcgt gcttggactg tccaagacgg tggtggaggg gacgctggtc aaggtgaagg
     1441 ccgcgattga cgaggaggcg aagctgaagc ttgccgtgca gagcgacctg gtgttcatca
     1501 cgggggaatt cgagatgatg cagtcgttcc tcaacgtcgc cgacgcggag cgcatcaaga
     1561 acaacgccgt caagacgtgg gtgcggcagc tccgcgacct ggcctacgac accgaagact
     1621 gcatcgagct ggtggtccac ctggatccca agcccagatg gtggcgccgc ctgctggtcc
     1681 tgccttgcct gccggcggtg tcgctgccca tggacaacgc ggcggcggag ataaaggagc
     1741 tcaaggaccg ggtggagttc gtgagccaga ggaacatgcg ctacaagctc atcaccgact
     1801 tcggcggcgc caagtccgtc gcgcagcagc agcttgaccg ggccgccgga gctctcgaca
     1861 tcgccgtgga ggaggcggcg aagaaaggtc gtagcttggt ggatctgacc gagttgatcc
     1921 ccagaatgga agaccgccct gagctcggcg tgatctcggt gtgggggacc ggcggcgacc
     1981 ttggggtggc gtccatcgtc aggaaggcct acgacgaccc tgaaatttgc aagaacttcc
     2041 atagtcgtgg ctgggccaag ttgacccacc ctttcgatcc gcggaagatc ctccggagct
     2101 tgttcattca gttctgcact aacactgctg ctccacagaa gcaaggggaa actctggatg
     2161 tagattccct tctaaggatg gagaagacgg tggtggaaga aggggagctg gtcaagcagt
     2221 ttgtgagtca tgtgagcacc cacaggttcc tcgttgtcct cgaaggcgtg tcaaccatgg
     2281 cggaatggga tgccctccgg atgtacctgc cagacatggg taacgggagc cagatcatcg
     2341 tatccacaca gcattttgac atcgcgagct tgtgcaccgg gcagctgcac aaagtttcag
     2401 agttgagaaa attcacacct gaccactctg tttgtgtctt tttcaaggaa gtttccggag
     2461 gtgatgatcc tggaaatgca atgttggagg gagatgacga aactgaactt attgggcgcg
     2521 acacagaaga ggacgagctt atggagcaga ttgatgaggt ttcccgtgtg atctccgtat
     2581 gggggattgc tggtgtagga aaatcggcac ttgtcagaag tgcctactgc aagtatctgc
     2641 aggaccgccc caacttcgcc gggttaaatc tgacctacat acacgggcgc gtgaacgtgc
     2701 cccatccctt caatctgagg gagttctgcc gcagcttgct cctgaacttg tcctctgaac
     2761 ctgctaaaac caaagaggac gccgtcgctg aactggcaaa catgaccaat ccaattgatg
     2821 tctgccgtaa gcttcttagc gacgatcaac agcagaagca gtgccttatt gttattgatg
     2881 acctgcagtc cactgaagaa tgggacagaa taaaacaagc cttgtcattt ggtaaagaat
     2941 gttgtatcat agttgttaca aatgattcaa gtgttgccaa atattgttca gggcaagatc
     3001 agaaactcat gttcaacgtc aaaggtcttg gtgacgagca tgcccttgac ctcttcaaca
     3061 cggtatatga cggcgtgggt aaccacatca cacgggatga ggacatgatc aatcagtcga
     3121 agctaatgtt aaacaaatgc ggcggactac ccaaggtgat agttgcaatt ggttgtttct
     3181 tggccaagag tctgaattgg gaggccatga atactaactt tattcaccag ttggaaaaca
     3241 acccggagct ggctagtgta catagcatgt ttagttggct agactcctac ttccacaatt
     3301 gtcctgacga actcaagcca tgcatcttct atttgtcaat cttccctcga cgccatggca
     3361 ttcgtcgaag gcgtctggtg aggcggtgga ttgccgaggg ctactcaaga gacaccgacg
     3421 ggaatctcgc ggaagtgaac ggggaaaatt acttctccag gctcgtcaac ctcagcatgc
     3481 tcacagatgc tgagcgtgat gtcaacgtgg ctgggacagc gactggggct ggtactggga
     3541 ggagaatggc catgtgcaac gtcaacgatt tcttccggga gtacattgtg tcgcggcgaa
     3601 tggaggagaa ccatgtgttt gcactggagg ggaggtgcag ccagaccacg cggcgcactg
     3661 gacggcacct ggtcatcgac aaaagctggg acggggacga gagcgtgttc aacaggatcg
     3721 agttctcgcg actgcggtcg gtgacggtgt tcggaccctg gaagccattc ctggcctccg
     3781 acaagatgag ggtgctccgc gtgcttgatc tggaaggcac tgagggtctg acagacaacg
     3841 acatcaagaa catcgtcaaa cggctgcctc gactaaagtt cctctccctg cgaggatgca
     3901 agcacatctt tcggctgcca aaatccctgg gtcgcctgag gcagctccag accctggatg
     3961 tgaggcacac tgccgtagcc gggctgccag cgaccgtcgt gaagctacag aagctgcagt
     4021 atgttcgtgc cggcaacaca ccagaggagt atgccgtcag cgacagcacg gaatctagca
     4081 cctcagtacc cacctgtttc tcatgtggcc ctgttggcaa tcacattgtc ggcgttgagg
     4141 tgcctcgcgg ggtcgagaat ctgaccaacc tgcacacact gggcgtcatc aaagccactg
     4201 tggcaggact caaggagctc aagaagctta cccagctgcg caagctcggc gtgtctggca
     4261 taaaccggag gaaccacaaa gagctctgcg ccgtcatctc gggccacggc catctggaat
     4321 ctctgtcgat atggtttgac aaggacaccg agaggattgc tgacccttgt tcgccgagca
     4381 atgatgactt caagcctcca gagaagctac ggaggctcaa actccatggg cacactgaca
     4441 acctgccagc atgggttagc actctcaaaa tatttgtcac tcaagatcaa tgagtcgacc
     4501 tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
     4561 ttcattgcgc acacaccaga atcctactga gttcgagtat tatggcattg ggaaacatgt
     4621 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
     4681 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
     4741 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaaatat
     4801 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
     4861 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca
     4921 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct
     4981 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc
     5041 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt
     5101 ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct tggcactggc
     5161 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5221 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5281 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
     5341 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
     5401 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc
     5461 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     5521 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     5581 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     5641 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     5701 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     5761 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     5821 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     5881 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     5941 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     6001 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     6061 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     6121 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     6181 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     6241 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     6301 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     6361 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     6421 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     6481 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     6541 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     6601 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     6661 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     6721 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     6781 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     6841 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     6901 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     6961 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     7021 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     7081 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     7141 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     7201 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     7261 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     7321 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     7381 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     7441 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     7501 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     7561 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     7621 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     7681 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
     7741 gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     7801 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     7861 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     7921 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     7981 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     8041 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     8101 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     8161 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     8221 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     8281 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     8341 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     8401 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     8461 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     8521 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     8581 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     8641 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     8701 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     8761 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     8821 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     8881 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     8941 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     9001 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     9061 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     9121 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     9181 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     9241 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     9301 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     9361 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     9421 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     9481 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9541 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     9601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
    10441 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
    10501 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
    10561 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
    10621 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
    10681 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
    10741 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
    10801 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
    10861 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
    10921 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
    10981 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
    11041 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
    11101 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
    11161 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
    11221 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
    11281 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
    11341 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
    11401 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
    11461 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
    11521 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
    11581 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    11641 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
    11701 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
    11761 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
    11821 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga
    11881 accctaattc ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc
    11941 gatcgacaga tccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg
    12001 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
    12061 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
    12121 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
    12181 aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct
    12241 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    12301 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    12361 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    12421 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    12481 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    12541 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    12601 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag
    12661 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
    12721 gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag
    12781 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    12841 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    12901 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    12961 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg gatctgcgaa
    13021 agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
    13081 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc
    13141 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    13201 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
    13261 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
    13321 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    13381 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
    13441 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac
    13501 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    13561 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    13621 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    13681 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    13741 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    13801 ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg
    13861 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    13921 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    13981 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    14041 gaccatgatt ac
//
