LOCUS       Exported                8259 bp ds-DNA     circular SYN 23-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          8056..8259
                     /note="/dnas_title=pICH41414"
     misc_feature    complement(56..203)
                     /label=TDNA-LB
                     /note="shows similarity to T-DNA left border: GenBank 
                     Accession Number J01825"
                     /note="/vntifkey=21"
     rep_origin      233..850
                     /direction=RIGHT
                     /standard_name="oriV"
                     /label=oriV
                     /note="shows similarity to GenBank Accession Number M20134"
                     /note="/vntifkey=33"
     CDS             1048..1905
                     /codon_start=1
                     /label=AP r
                     /label=AP\r
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(1913..2702)
                     /label=pUC ori
                     /label=pUC\ori
                     /note="/vntifkey=21"
     rep_origin      2667
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="/vntifkey=33"
     misc_feature    2713..4194
                     /label=RK2 trfa (no Esp3I)
                     /label=RK2\trfa\(no\Esp3I)
                     /note="/vntifkey=21"
     misc_feature    4218..4345
                     /label=RB short
                     /label=RB\short
                     /note="/vntifkey=21"
     promoter        4378..5863
                     /label=ZmUbi promoter fragment
     5'UTR           5187..5344
                     /label=ZmUbi1
     5'UTR           5268..5344
                     /label=ZmUbi1
     misc_feature    5889..6371
                     /label=AvrSr62-7
     MIsc._Feature   6395..6398
                     /label=TTCG 4bp overhang
     CDS             6399..8051
                     /codon_start=1
                     /label=C terminal Luciferase tag - whole coding sequen
                     /label=C terminal Luciferase tag - whole coding sequence of
                     Luciferase gene from Photinus pyralis
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     CDS             8052..8055
                     /codon_start=1
                     /label=GCTT
                     /note="/vntifkey=4"
                     /translation="A"
     terminator      8056..8259
                     /label=35S ter
                     /note="/dnas_title=35S ter"
                     /note="/vntifkey=43"
ORIGIN
        1 cgctaagctt gaattcttgc ttgtcttcac agagtggggc ccactgcatc caccccagta
       61 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
      121 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
      181 actcgataca ggcagcccat cagtcagatc aggatctcct ttgcgacgct caccgggctg
      241 gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg
      301 tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg
      361 gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg
      421 gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg
      481 caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg
      541 gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg
      601 cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt
      661 gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc
      721 caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac
      781 cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga
      841 accctcccgg cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc
      901 gaacggcctc accccaaaaa tggcagcgct ggccaattcg tgcgcggaac ccctatttgt
      961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1741 aaatctggag ccggtgagcg tggttctcgc ggtatcattg cagcactggg gccagatggt
     1801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2701 gcagatccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc
     2761 gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc
     2821 tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct
     2881 acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt
     2941 caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt
     3001 gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt
     3061 ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct
     3121 tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc
     3181 aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg
     3241 aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga
     3301 ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg
     3361 ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata
     3421 ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt
     3481 tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg
     3541 tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac
     3601 tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct
     3661 tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga
     3721 aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg
     3781 ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga
     3841 aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc
     3901 actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt
     3961 tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca
     4021 tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg
     4081 aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt
     4141 gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc agcgcctgat
     4201 ctggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
     4261 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
     4321 atatcctgtc aaacactgat agtttaaacc acttcgtgca gaagacaata gtggagggtc
     4381 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
     4441 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
     4501 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata
     4561 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac
     4621 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata
     4681 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata
     4741 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa
     4801 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga
     4861 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt
     4921 ttcgagtaga taatgccagc ctgttaaacg ccgtcgatcg acgagtctaa cggacaccaa
     4981 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg
     5041 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca
     5101 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc
     5161 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc
     5221 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt
     5281 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt
     5341 caaggtacgc cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg
     5401 gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg
     5461 tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct
     5521 gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc
     5581 agacgggatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     5641 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     5701 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     5761 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     5821 cttttgtcga tgctcaccct gttgtttggt gttacttctg cagaatgtac gcgtcccggg
     5881 gcggtaccat gaagccaatc atgaagtcct ccatcttcaa gggcggcgag actagctctc
     5941 tcggcgagtc tccattcgag ggcccactct acaagtacga ggaaatcgtg cacgcccaag
     6001 gcaagttcct cgtgaccacc tactacagga ccccaatcaa gctcgagttc ccattcaacg
     6061 gcaaggacat ctccatcagc ctcaacgagt tctccaagaa ctccgacagc ctcggcatct
     6121 ccaaggaaga gaaggaaaag gtcaccaaga ccatcaactc cgaaggcaac ggcgcccaat
     6181 cctccaccat gggccaaaac aagggcaagg aactcagcat cgaggaaaag gaaaagatct
     6241 ggccggacgg cccaccaagg ttcgacgaga agaagtacgg catcaagctc agggacgcca
     6301 acggcgacag gaccatcttt tacaagtact ctcacacccc atacgtggtg accgagctga
     6361 acttgaaccc agtcgacctg cagagctttc gtggttcgat ggaggacgcc aaaaacataa
     6421 agaaaggccc ggcgccattc tatcctctag aggatggaac cgctggagag caactgcata
     6481 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     6541 aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga
     6601 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     6661 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     6721 tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg
     6781 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga
     6841 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     6901 tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc
     6961 gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta cctaagggtg
     7021 tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg
     7081 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     7141 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     7201 ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag
     7261 taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     7321 atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg
     7381 caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag
     7441 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     7501 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag
     7561 gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     7621 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     7681 aggacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc
     7741 aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg
     7801 gcgtggcagg tctgcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     7861 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     7921 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     7981 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt
     8041 ccaaattgta agcttctcta gctagagtcg atcgacaagc tcgagtttct ccataataat
     8101 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     8161 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     8221 ctaattccta aaaccaaaat ccagtactaa aatccagat
//
