LOCUS       Exported                8460 bp ds-DNA     circular SYN 26-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             270..1415
                     /codon_start=1
                     /label=trfA
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             1442..1462
                     /codon_start=1
                     /label=TetR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=95.2"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
                     /translation="WGFSSQR"
     misc_feature    complement(1574..1598)
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1643..2449
                     /codon_start=1
                     /label=IBMP_CAMVB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=85.9"
                     /note="/match_length=51.5"
                     /note="/other=CDS"
                     /translation="GIPIPQKSELNSTVAPLRAESGIQHPHINYYVVYNGPHAGIYDDW
                     GCTKAATNGVPGVAHKKFATITEARAAADAYTTSQQTDRLNFIPKGEAQLKPKSFAKAL
                     TSPPKQKAHWLTLGTKRPSSDPAPKEISFAPEITMDDFLYLYDLGRKFEGEGDDTMFTT
                     DNEKVSLFNFRKNADPQMVREAYAASLIKTIYPSNNLQEIKYLPKKVKDAVKRFRTNCI
                     KNTEKDIFLKIRSTIPVWTIQGLLHKPRQVIEIGVSKKVVPTESKAM"
     promoter        2633..2978
                     /label=CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.4"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2930..2976
                     /label=minimal CaMV 35S promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3217..5439
                     /label=Sr62TK
     misc_feature    3217..4194
                     /label=Kinase 1
     misc_feature    4384..5322
                     /label=Kinase 2
     terminator      5473..5725
                     /label=NOS terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     misc_feature    complement(5836..5860)
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     rep_origin      5952..6583
                     /label=oriV
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(6234..6419)
                     /codon_start=1
                     /label=YPK6_ECOLX
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=98.4"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MLDFLPVDSPSNVNRCAPHLSALCPSSVKDRAPHLSVVAPLKCQY
                     RRALIPRLVHIICGKLA"
     promoter        6670..6774
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             6775..7635
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7806..8394
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     ncRNA           complement(7845..7949)
                     /label=RNAI
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=102.9"
                     /note="/other=ncRNA"
ORIGIN
        1 acaagcagga gcgcaccgac ttcttccgca tcaagtgttt tggctctcag gccgaggccc
       61 acggcaagta tttgggcaag gggtcgctgg tattcgtgca gggcaagatt cggaatacca
      121 agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat aaggtggatt
      181 atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt gccccggcgt
      241 gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca
      301 ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca
      361 ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta
      421 cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg
      481 ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc aggtttggcg aagtcgatga
      541 ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg
      601 caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca
      661 aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc
      721 caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg
      781 cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg
      841 tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc
      901 gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct
      961 ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga
     1021 cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc
     1081 gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg
     1141 aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca
     1201 agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc
     1261 cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt
     1321 ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg
     1381 cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg
     1441 ctgggggttc agcagccagc gcctgatctg gggaaccctg tggttggcat gcacatacaa
     1501 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
     1561 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaaccact
     1621 tcgtgcagaa gacaagtaag gaggaattcc aatcccacaa aaatctgagc ttaacagcac
     1681 agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact actacgttgt
     1741 gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg cggcaacaaa
     1801 cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa gagcagcagc
     1861 tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca aaggagaagc
     1921 tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc aaaaagccca
     1981 ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag agatctcctt
     2041 tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa ggaagttcga
     2101 aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc tcttcaattt
     2161 cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc tcatcaagac
     2221 aatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg ttaaagatgc
     2281 agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat ttctcaagat
     2341 cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa ggcaagtaat
     2401 agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc atggagtcta
     2461 agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag ttcatacaga
     2521 gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacactc
     2581 tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt
     2641 ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     2701 tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
     2761 gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca
     2821 cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat
     2881 gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     2941 cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata agagctcatt
     3001 tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca tttacaatta
     3061 tcgataccca tggaacaaaa gttgatctct gaagaggacc ttgagcagaa attgatctct
     3121 gaggaagatc ttgagcagaa gcttatctca gaggaggatt tggagcaaaa acttatttct
     3181 gaagaggacc taatgtacgc gtcccggggc ggtaccatgg acggatacga gttcgagagg
     3241 gcggagatag atgcactgga gcgcgtcgta cgggatccaa gagaaaagcc aataagcctg
     3301 tcgttttggc ttctcaggcg cataacgaat gatttctcta acgcatctga aattggtcga
     3361 ggtggatttt cgaacgttta cctgggggtg cttccaagtg ggttgcgtat tgctgtcaaa
     3421 aagctttacc aacttactgc attggctaat gaatttgaag atgaagttct taccacaatg
     3481 agggttgctc acaaaaacat agtgcgactc ataggctact gtcgtcacac gcaatctgaa
     3541 gtcttcgaat taaaaggaga atctattttc gcacaggtca gagaaaggtt gatctgtaca
     3601 gagtatgtgc ctaatggaac tcttgatgga catatcactg ataagtttca tggaggactt
     3661 gattgggacc agcgttatca agttctcaaa ggaatttgtc acggtttgca ttatctgcac
     3721 gatgaaatac gcgttatcca cagagatatc aaaacagaaa atatattgct agatgataac
     3781 ggcgtgccaa aagtttttga ctttggtttt tcgaagtgcc ttcctgatgg agaatcttta
     3841 attaaagatg aagcaaccca gggaacccta gggtatttgg cgccggagtc agttgataag
     3901 caagaatatt cctttaagtc cgacatatac agctttggtg ttgtgatcat acaattattg
     3961 actggatcgt ggatcagtaa gaaccgaaat gttgagctag aacttaagga gttgagaaaa
     4021 aggttggtaa gagaaggagc atttccatca tgggaaaaca aataccacca agttagaaca
     4081 tgtctggaga ttgtgtataa ctgcatggac atcgacccag ataaaaggcc gactgcattg
     4141 gaaattatcc aacggcttga tgaaaccgaa agcgcgaact attctgttag cagtggccat
     4201 gcaacaccac tttggcagtc aggagatgaa gaatccgact cagcggaagt ggatgctttt
     4261 gagccagaga gaacaccatc aaaagacatt ctttcagatg atgaggaacc cgcatcagca
     4321 gacgcaaatg gagaaacgag cacacaaaaa cataatatgc ctgatcttgt aagtaaacag
     4381 tcagtgtcgg cggaactgtc ctcgctagat ttccttgaga aaatcacaga tggtttttca
     4441 tacgagcaaa tactcgggaa ggaaagtctg gacgcagatg ttcataaagc atttctttat
     4501 gagggcagca tttcaggtcc agcaaaggta gttgtgaaga ggttaattgg agtagcaatt
     4561 ccagttgcga agtttaagag ggatgcagaa cagctgatga gcctggatca caagaatata
     4621 gtaaagctcc ttggctactg ccacgacgaa tctagagggc ataagctggt acagtttaaa
     4681 gcaaaaccac cgcaagattt taaaggagcc gagcaacttc tctgctatga atatatgcac
     4741 aatggaagcc tccgtgagta tcttactgga caaggatctc gtgaagttga ttggcacatg
     4801 tgctacaaat tgatcaaagg gacctgcgaa ggtttacgct acctccatga gggttgtgga
     4861 gatcgtccaa ttcttcattt gaatctaaac ccgtcaaata tactgctgga cgaaaacaac
     4921 ataccacgca tcacgggttt tgatttttcg aagctcattg gtgaaaagaa cacaaaatcc
     4981 gtggttctta agaagaatgg accactaggt tacctgccac cggacttcct gtactcaaag
     5041 ggtactgacc tcaagtatct acctacggtg gacatataca gcttgggtct tataatttta
     5101 gagattgcaa cacgtcaaga gatcaaggga gaccatggaa taattattag gactgtcgag
     5161 acaaactgga ggcaagatac acaaatagca acactgtatg gttcactaga agataaatta
     5221 cgggagcaag taaaaatgtg cattgacatt ggcctagact gtgtcaagtc aaaccctaaa
     5281 aagagaccta cagctggggc catcatgctc tggcttgaca aagggagcaa accgggagtt
     5341 gccagacctc cactccgcga taatgtcacc catgcagatg atcacatcca agaaaaggag
     5401 aagcagacgc cattgaggag atttttcaga agaaagtaag tcgacctgca gagctttcgt
     5461 gcttgtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     5521 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     5581 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     5641 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     5701 gcggtgtcat ctatgttact agatcgacgc tgagctcgaa ttctagtttg tcttcacaga
     5761 gtggggccca ctgcatccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
     5821 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     5881 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tcagatcagg
     5941 atctcctttg cgacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
     6001 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg
     6061 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     6121 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     6181 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
     6241 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     6301 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     6361 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     6421 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     6481 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     6541 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc
     6601 cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc
     6661 caattcgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6721 ctcatgagac aataaccttg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ttctcgcggt
     7501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7921 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8401 agcaacgcgg cctttttacg gttcctggca gatcctagat gtggcgcaac gatgccggcg
//
