LOCUS       Exported                9574 bp ds-DNA     circular SYN 31-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..229
                     /label=RSV promoter
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
     promoter        1816..2342
                     /label=CMV promoter
     misc_feature    1820..2107
                     /label=CAG enhancer
     misc_feature    2274..2294
                     /label=CMV FWD (LSlabs)
     primer_bind     2277..2294
                     /label=oJDW 462
     protein_bind    2440..2464
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2466..2556
                     /label=oJDW 1718 - BglII_V5_TCF7_FWD
     misc_feature    2471..2480
                     /label=Kozak Sequence
     CDS             2480..2521
                     /codon_start=1
                     /label=V5_Tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     2536..2555
                     /label=JDW 1688 BglII_TCF7_FWD
     CDS             2537..3691
                     /codon_start=1
                     /label=TCF7
                     /translation="MPQLDSGGGGAGGGDDLGAPDELLAFQDEGEEQDDKSRDSAAGPE
                     RDLAELKSSLVNESEGAAGGAGIPGVPGAGAGARGEAEALGREHAAQRLFPDKLPEPLE
                     DGLKAPECTSGMYKETVYSAFNLLMHYPPPSGAGQHPQPQPPLHKANQPPHGVPQLSLY
                     EHFNSPHPTPAPADISQKQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGS
                     GVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAKKPTIKKPLNAFMLYMK
                     EMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARD
                     NYGKKKRRSREKHQESTTGGKRNAFGTYPEKAAAPAPFLPMTVLL"
     misc_feature    2786..2852
                     /label=exon 2
     primer_bind     2821..2839
                     /label=Primer1
     misc_feature    2853..2977
                     /label=exon 3
     primer_bind     complement(2899..2918)
                     /label=Primer 2
     misc_feature    2978..3083
                     /label=exon4
     primer_bind     complement(3665..3697)
                     /label=JDW 1689 KpnI_TCF7_REV
     CDS             3701..3757
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3733..3783
                     /label=oJDW 1017 (P2A_AvrII_H2A_FWD)
     primer_bind     3733..3783
                     /label=oJDW 1017
     regulatory      3759..3766
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3764..4147
                     /codon_start=1
                     /label=H2A.F/Z
                     /translation="MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKTRTTSHG
                     RVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKAT
                     IAGGGVIPHIHKSLIGKKGQQKTA"
     primer_bind     3764..3783
                     /label=oJDW 1370
     CDS             4154..4864
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4154..4861
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4840..4861)
                     /label=oJDW 1371
     primer_bind     complement(4842..4902)
                     /label=oJDW 1018
     protein_bind    complement(4882..4906)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4959..5000
                     /codon_start=1
                     /label=V5 TAG invitrogen
                     /translation="GKPIPNPLLGLDST"
     promoter        5033..5530
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        5544..5591
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5610..6008
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6095..6328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6400..6521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(6437..6456)
                     /label=SV40pA REVSeq
     rep_origin      6561..6696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    complement(6717..6735)
                     /label=T7 promoter
     misc_feature    complement(6745..6761)
                     /label=M13 For -20
     CDS             6833..6901
                     /label=LacZ alpha
     rep_origin      6903..7358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7384..7488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7489..8349
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8520..9108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9396..9417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9432..9462
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9470..9486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9494..9514
                     /label=M13-rev
     promoter        9531..9549
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2461 ggctggatct gccaccatgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     2521 gggtggtgct ggtggtatgc cgcagctgga ctccggcggg ggcggcgcgg gcggcggcga
     2581 cgacctcggc gcgccggacg agctgctggc cttccaggat gaaggcgagg agcaggacga
     2641 caagagccgc gacagcgccg ccggtcccga gcgcgacctg gccgagctca agtcgtcgct
     2701 cgtgaacgag tccgagggcg cggccggcgg cgcagggatc ccgggggtcc cgggggccgg
     2761 cgccggggcc cgcggcgagg ccgaggctct cgggcgggaa cacgctgcgc agagactctt
     2821 cccggacaaa cttccagagc ccctggagga cggcctgaag gccccggagt gcaccagcgg
     2881 catgtacaaa gagaccgtct actccgcctt caatctgctc atgcattacc cacccccctc
     2941 gggagcaggg cagcaccccc agccgcagcc cccgctgcac aaggccaatc agccccccca
     3001 cggtgtcccc caactctctc tctacgaaca tttcaacagc ccacatccca cccctgcacc
     3061 tgcggacatc agccagaagc aagttcacag gcctctgcag acccctgacc tctctggctt
     3121 ctactccctg acctcaggca gcatggggca gctcccccac actgtgagct ggttcaccca
     3181 cccatccttg atgctaggtt ctggtgtacc tggtcaccca gcagccatcc cccacccggc
     3241 cattgtgccc ccctcaggga agcaggagct gcagcccttc gaccgcaacc tgaagacaca
     3301 agcagagtcc aaggcagaga aggaggccaa gaagccaacc atcaagaagc ccctcaatgc
     3361 cttcatgctg tacatgaagg agatgagagc caaggtcatt gcagagtgca cacttaagga
     3421 gagcgctgcc atcaaccaga tcctgggccg caggtggcac gcgctgtcgc gagaagagca
     3481 ggccaagtac tatgagctgg cccgcaagga gaggcagctg cacatgcagc tatacccagg
     3541 ctggtcagcg cgggacaact acgggaagaa gaagaggcgg tcgagggaaa agcaccaaga
     3601 atccaccaca ggaggaaaaa gaaatgcatt cggtacttac ccggagaagg ccgctgcccc
     3661 agccccgttc cttccgatga cagtgctctt gggtaccgga gccactaact tctccctgtt
     3721 gaaacaagca ggggatgtcg aagagaatcc cgggccacct aggatggcag gtggaaaagc
     3781 aggtaaagac agtggaaaag ccaagacgaa agccgtctcc agatctcaga gagctggtct
     3841 gcagttccct gtaggtcgta tccacaggca cctgaagacc cgaaccacca gccatggccg
     3901 tgtcggagct acagctgcgg tttacagtgc tgctatcctg gagtatctca ccgctgaggt
     3961 gctggagttg gctggaaacg cttctaagga tctgaaggtg aagcgaatca ctcccagaca
     4021 tttacagctg gccatccgcg gagacgagga gctggactcg cttatcaagg ccaccattgc
     4081 tggaggagga gtcattcctc acatccacaa gtctctgatt ggtaagaagg gccagcagaa
     4141 aaccgcaggg gggatggtga gcaagggcga ggaggacaac atggccatca tcaaggagtt
     4201 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     4261 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     4321 cggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     4381 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     4441 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     4501 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     4561 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     4621 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     4681 ccactacgac gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     4741 cgcctacaac gtcaacatca agctggacat cacctcccac aacgaggact acaccatcgt
     4801 ggaacagtac gagcgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     4861 gtaatgatag ctcgagagat ctcccagctt tcttgtacaa agtggttgat atccagcaca
     4921 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     4981 tcctcggtct cgattctacg cgtaccggtt agtaatgacg atggtaccta ccgggtaggg
     5041 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     5101 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     5161 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     5221 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     5281 agtctcgtgc agatggacag caccgctgag caatggaagc ggtaggcctt tggggcagcg
     5341 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     5401 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaagtcct ccggaggccc
     5461 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     5521 gggcctttcg actctagaca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     5581 tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca
     5641 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     5701 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     5761 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     5821 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     5881 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     5941 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     6001 agggctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     6061 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     6121 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6181 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6241 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6301 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6361 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     6421 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6481 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     6541 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6601 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6661 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     6721 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6781 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6841 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6901 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6961 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7021 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7081 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7141 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7201 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7261 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7321 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     7381 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7441 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7501 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7561 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7621 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7681 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7741 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7801 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7861 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7921 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7981 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8041 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8101 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8161 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8221 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8281 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8341 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8401 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8461 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8641 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     8761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8881 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8941 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9001 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9061 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9301 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9361 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     9421 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     9481 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     9541 cactaaaggg aacaaaagct ggagctgcaa gctt
//
