LOCUS       Exported               10173 bp ds-DNA     circular SYN 31-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..229
                     /label=RSV promoter
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
     promoter        1816..2342
                     /label=CMV promoter
     misc_feature    1820..2107
                     /label=CAG enhancer
     misc_feature    2274..2294
                     /label=CMV FWD (LSlabs)
     primer_bind     2277..2294
                     /label=oJDW 462
     protein_bind    2440..2464
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2455..2552
                     /label=oJDW 1719 - CF_V5_TCF7L1_FWD
     CDS             2479..2520
                     /codon_start=1
                     /label=V5
                     /translation="GKPIPNPLLGLDST"
     primer_bind     2527..2551
                     /label=oJDW 1690 TCF7L1_CF_FWD
     CDS             2536..4296
                     /codon_start=1
                     /label=TCF7L1
                     /note="cDNA clone MGC:64931 "
                     /translation="MPQLGGGGGGGGGSGGGGGSSAGAAGGGDDLGANDELIPFQDEGG
                     EEQEPSSDSASAQRDLDEVKSSLVNESENQSSSSDSEAERRPQPVRDTFQKPRDYFAEV
                     RRPQDSAFFKGPPYPGYPFLMIPDLSSPYLSNGPLSPGGARTYLQMKWPLLDVPSSATV
                     KDTRSPSPAHLSNKVPVVQHPHHMHPLTPLITYSNDHFSPGSPPTHLSPEIDPKTGIPR
                     PPHPSELSPYYPLSPGAVGQIPHPLGWLVPQQGQPMYSLPPGGFRHPYPALAMNASMSS
                     LVSSRFSPHMVAPAHPGLPTSGIPHPAIVSPIVKQEPAPPSLSPAVSVKSPVTVKKEEE
                     KKPHVKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRKWHNLSREEQAKYYELAR
                     KERQLHSQLYPTWSARDNYGKKKKRKREKQLSQTQSQQQVQEAEGALASKSKKPCVQYL
                     PPEKPCDSPASSHGSMLDSPATPSAALASPAAPAATHSEQAQPLSLTTKPETRAQLALH
                     SAAFLSAKAAASSSRQMGSQPPLLSRPLPLGSMPTALLASPPSFPATLHAHQALPVLQA
                     QPLSLVTKSAH"
     primer_bind     complement(2636..2658)
                     /label=TCF7L1_5Prime_REV
     primer_bind     2773..2792
                     /label=oJDW 1710 TCF7L1_5Prime_FWD
     misc_feature    2782..2845
                     /label=Exon2
     misc_feature    2846..2973
                     /label=Exon3
     primer_bind     2926..2943
                     /label=Primer 1
     misc_feature    2974..3057
                     /label=Exon 4
     primer_bind     complement(3021..3039)
                     /label=Primer 2
     primer_bind     3030..3053
                     /label=oJDW 1783 - TCF7L1_cDNA_FWD3
     primer_bind     3359..3379
                     /label=oJDW 1711 TCF7L1_Middle_FWD
     primer_bind     4183..4202
                     /label=oJDW 1708 TCF7L1_3Prime_FWD
     primer_bind     complement(4184..4202)
                     /label=oJDW 1702_TCF7L1_3Prime_REV
     primer_bind     complement(4267..4310)
                     /label=oJDW 1691 TCF7L1_CF_REV
     primer_bind     complement(4277..4310)
                     /label=oJDW 1720 - CF_TCF7L1_REV
     CDS             4300..4356
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     4332..4382
                     /label=oJDW 1017 (P2A_AvrII_H2A_FWD)
     primer_bind     4332..4382
                     /label=oJDW 1017
     regulatory      4358..4365
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4363..4746
                     /codon_start=1
                     /label=H2A.F/Z
                     /translation="MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKTRTTSHG
                     RVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKAT
                     IAGGGVIPHIHKSLIGKKGQQKTA"
     primer_bind     4363..4382
                     /label=oJDW 1370
     CDS             4753..5463
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5439..5460)
                     /label=oJDW 1371
     primer_bind     complement(5441..5501)
                     /label=oJDW 1018
     protein_bind    complement(5481..5505)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5558..5599
                     /codon_start=1
                     /label=V5 TAG invitrogen
                     /translation="GKPIPNPLLGLDST"
     promoter        5632..6129
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        6143..6190
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6209..6607
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6694..6927
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6999..7120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(7036..7055)
                     /label=SV40pA REVSeq
     rep_origin      7160..7295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    complement(7316..7334)
                     /label=T7 promoter
     misc_feature    complement(7344..7360)
                     /label=M13 For -20
     CDS             7432..7500
                     /label=LacZ alpha
     rep_origin      7502..7957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7983..8087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8088..8948
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9119..9707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9995..10016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10031..10061
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10069..10085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10093..10113
                     /label=M13-rev
     promoter        10130..10148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2461 ggctggatcg ccaccatggg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2521 ggtggtgctg gtggtatgcc ccagctcggc ggcgggggcg gcggcggcgg cggcagcggg
     2581 ggaggcggcg gctccagcgc cggggcggcc ggcggagggg acgacctcgg ggcgaacgac
     2641 gagctgatcc ccttccagga cgaggggggc gaggagcagg agccgagcag cgatagcgcc
     2701 tcggcgcagc gggacctaga cgaggtcaag tcgtccctgg tcaacgagtc ggagaaccag
     2761 agcagcagct cggactcgga ggcggagagg cgcccgcagc ccgtccggga cactttccag
     2821 aagccgcggg actatttcgc cgaagtgaga aggcctcagg acagcgcgtt ctttaaagga
     2881 cccccgtacc ctgggtaccc cttcctgatg atcccggacc tgagcagccc gtacctctcc
     2941 aacggacccc tgtctcccgg aggagcgcgc acctacctgc agatgaaatg gcccctcctc
     3001 gatgtcccct ccagcgccac agtcaaggac acgaggtcac catctccagc acacttgtct
     3061 aataaagttc ctgtcgttca gcacccgcat cacatgcatc cgctgactcc cctcatcacc
     3121 tacagcaatg accacttctc ccccggctcc cctcccaccc acctctcccc agagatcgat
     3181 ccaaagacag gaatcccccg gccccctcac ccatccgagc tgtcaccgta ttacccactc
     3241 tctcccggag ctgtcggaca aatcccccac cccctcggct ggctcgtccc acagcaaggc
     3301 cagcccatgt actcccttcc tcccggtggc ttccggcacc cttaccccgc cctcgccatg
     3361 aacgcctcga tgtccagcct ggtctccagt cggttctctc ctcacatggt ggctcctgcc
     3421 caccctggcc tgcccacctc agggatcccc caccctgcca tcgtctcccc catcgtcaag
     3481 caggaaccgg caccccccag cctgagccct gcagtgagcg tgaaatcacc agtcaccgtg
     3541 aaaaaggagg aggaaaagaa gccccacgtg aagaagcctc tgaatgcctt catgttgtat
     3601 atgaaggaga tgagggccaa ggtggtggct gagtgcaccc tgaaggaaag tgcagccatt
     3661 aaccagatcc ttggaagaaa gtggcacaac ctgtctcgag aagaacaggc caagtactac
     3721 gagctggccc ggaaggagcg gcagcttcac tcgcagctct acccaacctg gtcagcccgg
     3781 gacaactatg gtaagaaaaa gaagaggaag agagaaaagc agctgtccca gacacagtca
     3841 cagcagcaag tccaggaggc agagggtgcc ctggcctcca agagcaagaa gccatgtgtt
     3901 cagtacctgc cccccgagaa gccctgtgac agccctgcct cctcccacgg gagcatgctg
     3961 gactccccgg ccactccctc tgcagctttg gcctcaccag ctgcccctgc tgccacccat
     4021 tcggagcaag cccagcccct ctccctcacc accaaaccag aaacccgggc ccagctggct
     4081 ctccactctg ccgccttcct gtcggctaag gctgcagcct cctcctctag gcagatgggc
     4141 agccagcctc ccctcctgtc ccggcccctc ccccttgggt ccatgcccac agctctgctg
     4201 gcctctcccc cgtccttccc cgccacgctc catgcccacc aggccctccc ggtgctacag
     4261 gcccagcctc tttccctggt caccaagtct gcccacggag ccactaactt ctccctgttg
     4321 aaacaagcag gggatgtcga agagaatccc gggccaccta ggatggcagg tggaaaagca
     4381 ggtaaagaca gtggaaaagc caagacgaaa gccgtctcca gatctcagag agctggtctg
     4441 cagttccctg taggtcgtat ccacaggcac ctgaagaccc gaaccaccag ccatggccgt
     4501 gtcggagcta cagctgcggt ttacagtgct gctatcctgg agtatctcac cgctgaggtg
     4561 ctggagttgg ctggaaacgc ttctaaggat ctgaaggtga agcgaatcac tcccagacat
     4621 ttacagctgg ccatccgcgg agacgaggag ctggactcgc ttatcaaggc caccattgct
     4681 ggaggaggag tcattcctca catccacaag tctctgattg gtaagaaggg ccagcagaaa
     4741 accgcagggg ggatggtgag caagggcgag gaggacaaca tggccatcat caaggagttc
     4801 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     4861 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     4921 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     4981 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     5041 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     5101 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     5161 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     5221 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     5281 cactacgacg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     5341 gcctacaacg tcaacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     5401 gaacagtacg agcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     5461 taatgatagc tcgagagatc tcccagcttt cttgtacaaa gtggttgata tccagcacag
     5521 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     5581 cctcggtctc gattctacgc gtaccggtta gtaatgacga tggtacctac cgggtagggg
     5641 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     5701 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     5761 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     5821 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     5881 gtctcgtgca gatggacagc accgctgagc aatggaagcg gtaggccttt ggggcagcgg
     5941 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     6001 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaagtcctc cggaggcccg
     6061 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     6121 ggcctttcga ctctagacac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     6181 ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac
     6241 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     6301 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     6361 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     6421 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     6481 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     6541 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     6601 gggctaagca caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     6661 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6721 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6781 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6841 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6901 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6961 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7261 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7321 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7381 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7441 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7501 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7561 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7621 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7681 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7741 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7801 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7861 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7921 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     7981 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8041 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8101 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8161 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8221 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8281 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8341 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8401 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8461 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8521 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8581 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8641 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8701 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8761 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8821 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9241 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9781 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9841 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9901 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9961 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10021 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10081 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10141 actaaaggga acaaaagctg gagctgcaag ctt
//
