LOCUS       Exported                9787 bp ds-DNA     circular SYN 31-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Baylor-NRI
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 27, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..229
                     /label=RSV promoter
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=HIV-1 Rev response element (RRE)
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1816..2342
                     /label=CMV promoter
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    1820..2107
                     /label=CAG enhancer
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2274..2294
                     /label=CMV FWD (LSlabs)
     primer_bind     2277..2294
                     /label=oJDW 462
     protein_bind    2440..2464
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2465..2556
                     /label=oJDW 1725 BamHI_TCF7L2_FWD
     regulatory      2471..2480
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2480..2521
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2537..3902
                     /codon_start=1
                     /label=TCF7L2
                     /translation="MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVK
                     SSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYP
                     FIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSN
                     KVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPL
                     SPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTG
                     IPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAK
                     VVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKK
                     KKRKRDKQPGETNEHSECFLNPCLSLPPITGEKKSAFATYKVKAAASAHPLQMEAY"
     misc_feature    2537..2725
                     /label=EXON1
     primer_bind     2704..2722
                     /label=Primer 1
     misc_feature    2726..2792
                     /label=EXON2
     misc_feature    2793..2917
                     /label=EXON3
     primer_bind     complement(2797..2815)
                     /label=Primer 2
     primer_bind     3253..3279
                     /label=oJDW 1703_TCF7L2_Mid_FWD
     primer_bind     complement(3872..3910)
                     /label=oJDW 1726 KpnI_TCF7L2_REV
     CDS             3914..3970
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3946..3996
                     /label=oJDW 1017 (P2A_AvrII_H2A_FWD)
     primer_bind     3946..3996
                     /label=oJDW 1017
     regulatory      3972..3979
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3977..4360
                     /codon_start=1
                     /label=H2A.F/Z
                     /translation="MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKTRTTSHG
                     RVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKAT
                     IAGGGVIPHIHKSLIGKKGQQKTA"
     primer_bind     3977..3996
                     /label=oJDW 1370
     CDS             4367..5077
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4367..5074
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5053..5074)
                     /label=oJDW 1371
     primer_bind     complement(5055..5115)
                     /label=oJDW 1018
     protein_bind    complement(5095..5119)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5172..5213
                     /codon_start=1
                     /label=V5 TAG invitrogen
                     /translation="GKPIPNPLLGLDST"
     promoter        5246..5743
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        5757..5804
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5823..6221
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6308..6541
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6613..6734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(6650..6669)
                     /label=SV40pA REVSeq
     rep_origin      6774..6909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    complement(6930..6948)
                     /label=T7 promoter
     promoter        complement(6930..6948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(6958..6974)
                     /label=M13 For -20
     primer_bind     complement(6958..6974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7046..7114
                     /label=LacZ alpha
     rep_origin      7116..7571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7597..7701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7702..8562
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8733..9321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9609..9630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9645..9675
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9683..9699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9707..9727
                     /label=M13-rev
     primer_bind     9707..9723
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9744..9762
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2461 ggctggatcc gccaccatgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     2521 gggtggtgct ggtggtatgc cgcagctgaa cggcggtgga ggggatgacc taggcgccaa
     2581 cgacgaactg atttccttca aagacgaggg cgaacaggag gagaagagct ccgaaaactc
     2641 ctcggcagag agggatttag ctgatgtcaa atcgtctcta gtcaatgaat cagaaacgaa
     2701 tcaaaacagc tcctccgatt ccgaggcgga aagacggcct ccgcctcgct ccgaaagttt
     2761 ccgagacaaa tcccgggaaa gtttggaaga agcggccaag aggcaagatg gagggctctt
     2821 taaggggcca ccgtatcccg gctacccctt catcatgatc cccgacctga cgagccccta
     2881 cctccccaac ggatcgctct cgcccaccgc ccgaacctat ctccagatga aatggccact
     2941 gcttgatgtc caggcaggga gcctccagag tagacaagcc ctcaaggatg cccggtcccc
     3001 atcaccggca cacattgtct ctaacaaagt gccagtggtg cagcaccctc accatgtcca
     3061 ccccctcacg cctcttatca cgtacagcaa tgaacacttc acgccgggaa acccacctcc
     3121 acacttacca gccgacgtag accccaaaac aggaatccca cggcctccgc accctccaga
     3181 tatatccccg tattacccac tatcgcctgg caccgtagga caaatccccc atccgctagg
     3241 atggcaaggt caaccagtgt acccaatcac gacaggagga ttcagacacc cctaccccac
     3301 agctctgacc gtcaatgctt ccatgtccag gttccctccc catatggtcc caccacatca
     3361 tacgctacac acgacgggca ttccgcatcc ggccatagtc acaccaacag tcaaacagga
     3421 atcgtcccag agtgatgtcg gctcactcca tagttcaaag catcaggact ccaaaaagga
     3481 agaagaaaag aagaagcccc acataaagaa acctcttaat gcattcatgt tgtatatgaa
     3541 ggaaatgaga gcaaaggtcg tagctgagtg cacgttgaaa gaaagcgcgg ccatcaacca
     3601 gatccttggg cggaggtggc atgcactgtc cagagaagag caagcgaaat actacgagct
     3661 ggcccggaag gagcgacagc ttcatatgca actgtacccc ggctggtccg cgcgggataa
     3721 ctatggaaag aagaagaaga ggaaaaggga caagcagccg ggagagacca atgaacacag
     3781 cgaatgtttc ctaaatcctt gcctttcact tcctccgatt acaggagaaa aaaaaagtgc
     3841 gttcgctaca tacaaggtga aggcagctgc ctcagcccac cctcttcaga tggaagctta
     3901 ctgcggtacc ggagccacta acttctccct gttgaaacaa gcaggggatg tcgaagagaa
     3961 tcccgggcca cctaggatgg caggtggaaa agcaggtaaa gacagtggaa aagccaagac
     4021 gaaagccgtc tccagatctc agagagctgg tctgcagttc cctgtaggtc gtatccacag
     4081 gcacctgaag acccgaacca ccagccatgg ccgtgtcgga gctacagctg cggtttacag
     4141 tgctgctatc ctggagtatc tcaccgctga ggtgctggag ttggctggaa acgcttctaa
     4201 ggatctgaag gtgaagcgaa tcactcccag acatttacag ctggccatcc gcggagacga
     4261 ggagctggac tcgcttatca aggccaccat tgctggagga ggagtcattc ctcacatcca
     4321 caagtctctg attggtaaga agggccagca gaaaaccgca ggggggatgg tgagcaaggg
     4381 cgaggaggac aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4441 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4501 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     4561 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     4621 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     4681 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     4741 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     4801 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     4861 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgccgagg tcaagaccac
     4921 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagctgga
     4981 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggccg
     5041 ccactccacc ggcggcatgg acgagctgta caagtaatga tagctcgaga gatctcccag
     5101 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     5161 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     5221 gttagtaatg acgatggtac ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     5281 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     5341 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     5401 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     5461 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     5521 gagcaatgga agcggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     5581 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     5641 gcggggcggg cgcccgaagt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     5701 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgactctag acacgtgttg
     5761 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     5821 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     5881 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     5941 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     6001 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     6061 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     6121 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     6181 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacaattc gagctcggta
     6241 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6301 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6361 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6421 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6481 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6541 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6601 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6661 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6721 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6781 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6841 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6901 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     6961 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7021 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7081 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     7141 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7201 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7261 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7321 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7381 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7441 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7501 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7561 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7621 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7681 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7741 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7801 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7861 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7921 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7981 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8041 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8101 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8161 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8221 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8281 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8341 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8401 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8461 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8521 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8581 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8641 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8701 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8761 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8821 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8881 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8941 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9001 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9061 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9121 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9181 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9241 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9301 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9361 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9421 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9481 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9541 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9601 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     9661 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9721 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     9781 caagctt
//
