LOCUS       Exported                5419 bp ds-DNA     circular SYN 09-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Iwasaki RNA systems biochemistry
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 28, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     150..172
                     /label=012 SV40-F
     primer_bind     222..244
                     /label=012 SV40-F
     primer_bind     complement(399..419)
                     /label=013 SV40-R
     primer_bind     607..624
                     /label=SI003/psiC-5UTR-seq
                     /note="psiCHECK2に入れたinsertを読むのに使う"
     misc_binding    666..684
     misc_feature    666..684
                     /label=T7 promoter
     primer_bind     666..684
                     /label=T7 primer
     primer_bind     666..684
                     /label=T7 promoter
     5'UTR           685..705
                     /label=EIF2S3 5UTR
     CDS             706..1638
                     /codon_start=1
                     /label=hRluc-Renilla
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     primer_bind     706..726
                     /label=006 hRluc ATG-F
                     /note="psiCHECK2のhRlucの頭のprimerです。004"
     primer_bind     complement(841..858)
                     /label=SI004/hRluc-toe
                     /note="psiCHECK2に入れたinsertを後ろから読むprimer"
     primer_bind     1088..1106
                     /label=SI044
     primer_bind     1124..1143
                     /label=SI049 qPCR
     primer_bind     complement(1168..1209)
                     /label=004 hRluc EcoRV-2R
                     /note="psiCHECK2ベクターをEcoRVで切った時にG.Aでつながるprimerです。"
     primer_bind     1190..1209
                     /label=121 hRluc seq-1F
     primer_bind     complement(1324..1343)
                     /label=SI050 qPCR
     primer_bind     1569..1587
                     /label=SI045
     primer_bind     complement(1616..1670)
                     /label=Fw
     primer_bind     complement(1618..1638)
                     /label=SI035
     primer_bind     1645..1701
                     /label=Rev
     primer_bind     1677..1701
                     /label=110 HCF_IRES_FL hluc-F
     CDS             1678..3330
                     /codon_start=1
                     /label=hluc+ FL
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     1678..1700
                     /label=SI034
     primer_bind     1681..1704
                     /label=SI081
     primer_bind     complement(1683..1704)
                     /label=111 Ffluc-1R
     primer_bind     2090..2107
                     /label=SI046
     primer_bind     2249..2270
                     /label=112 Ffluc-1F
     primer_bind     2560..2579
                     /label=SI051 qPCR
     primer_bind     2570..2589
                     /label=SI047
     primer_bind     complement(2713..2732)
                     /label=SI052
     primer_bind     3051..3069
                     /label=SI048
     primer_bind     complement(3358..3382)
                     /label=Fluc R　SI016
     primer_bind     complement(3358..3382)
                     /label=Fluc Rev
     terminator      3365..3586
                     /label=SV40 late poly(A)
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggtttcct tcctcttttg gcaacatggc ttccaaggtg
      721 tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag
      781 caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac
      841 gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct
      901 cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc
      961 aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc
     1021 gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg
     1081 gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt
     1141 gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgccctg
     1201 atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt cgagaccatg
     1261 ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta cctggagcca
     1321 ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga gatccctctc
     1381 gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc ctaccttcgg
     1441 gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct
     1501 attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac
     1561 ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc
     1621 gtgctgaaga acgagcagat gtcgacgggg gcccctagga gatcccccgg ggagctcatg
     1681 gccgatgcta agaacattaa gaagggccct gctcccttct accctctgga ggatggcacc
     1741 gctggcgagc agctgcacaa ggccatgaag aggtatgccc tggtgcctgg caccattgcc
     1801 ttcaccgatg cccacattga ggtggacatc acctatgccg agtacttcga gatgtctgtg
     1861 cgcctggccg aggccatgaa gaggtacggc ctgaacacca accaccgcat cgtggtgtgc
     1921 tctgagaact ctctgcagtt cttcatgcca gtgctgggcg ccctgttcat cggagtggcc
     1981 gtggcccctg ctaacgacat ttacaacgag cgcgagctgc tgaacagcat gggcatttct
     2041 cagcctaccg tggtgttcgt gtctaagaag ggcctgcaga agatcctgaa cgtgcagaag
     2101 aagctgccta tcatccagaa gatcatcatc atggactcta agaccgacta ccagggcttc
     2161 cagagcatgt acacattcgt gacatctcat ctgcctcctg gcttcaacga gtacgacttc
     2221 gtgccagagt ctttcgacag ggacaaaacc attgccctga tcatgaacag ctctgggtct
     2281 accggcctgc ctaagggcgt ggccctgcct catcgcaccg cctgtgtgcg cttctctcac
     2341 gcccgcgacc ctattttcgg caaccagatc atccccgaca ccgctattct gagcgtggtg
     2401 ccattccacc acggcttcgg catgttcacc accctgggct acctgatttg cggctttcgg
     2461 gtggtgctga tgtaccgctt cgaggaggag ctgttcctgc gcagcctgca agactacaaa
     2521 attcagtctg ccctgctggt gccaaccctg ttcagcttct tcgctaagag caccctgatc
     2581 gacaagtacg acctgtctaa cctgcacgag attgcctctg gcggcgcccc actgtctaag
     2641 gaggtgggcg aagccgtggc caagcgcttt catctgccag gcatccgcca gggctacggc
     2701 ctgaccgaga caaccagcgc cattctgatt accccagagg gcgacgacaa gcctggcgcc
     2761 gtgggcaagg tggtgccatt cttcgaggcc aaggtggtgg acctggacac cggcaagacc
     2821 ctgggagtga accagcgcgg cgagctgtgt gtgcgcggcc ctatgattat gtccggctac
     2881 gtgaataacc ctgaggccac aaacgccctg atcgacaagg acggctggct gcactctggc
     2941 gacattgcct actgggacga ggacgagcac ttcttcatcg tggaccgcct gaagtctctg
     3001 atcaagtaca agggctacca ggtggcccca gccgagctgg agtctatcct gctgcagcac
     3061 cctaacattt tcgacgccgg agtggccggc ctgcccgacg acgatgccgg cgagctgcct
     3121 gccgccgtcg tcgtgctgga acacggcaag accatgaccg agaaggagat cgtggactat
     3181 gtggccagcc aggtgacaac cgccaagaag ctgcgcggcg gagtggtgtt cgtggacgag
     3241 gtgcccaagg gcctgaccgg caagctggac gcccgcaaga tccgcgagat cctgatcaag
     3301 gctaagaaag gcggcaagat cgccgtgtaa taattctaga gtcggggcgg ccggccgctt
     3361 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     3421 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     3481 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     3541 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     3601 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3661 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3721 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3781 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3841 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3901 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3961 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4021 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4081 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4141 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4201 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4261 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4321 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4381 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4441 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4501 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4561 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4621 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4681 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5221 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5401 tttgctcaca tggctcgac
//
