LOCUS       plasmid                 8078 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     CDS             complement(2796..3515)
                     /note="pLannotate"
                     /label="EGFP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(7446..7825)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     CDS             complement(3952..4743)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5209..6000)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(4892..5026)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(3635..3769)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(1741..1875)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        7856..7960
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          complement(6151..7167)
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             join(7961..8078,1..743)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      914..1502
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(2589..2644)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     protein_bind    complement(4786..4819)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(3593..3626)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(6043..6076)
                     /note="pLannotate"
                     /label="FRT (minimal)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(4850..4883)
                     /note="pLannotate"
                     /label="FRT (minimal)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        2150..2180
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(7168..7444)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    2114..2135
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    2188..2204
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(1895..2029)
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3776..3928)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5033..5185)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3529..3565)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="69.8"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2715..2786)
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             1685..1739
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2033..2055)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             2062..2101
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
       61 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      121 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      181 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      241 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      301 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      361 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      421 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      481 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
      541 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
      601 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
      661 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
      721 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
      781 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
      841 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      901 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      961 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1021 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1081 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1141 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1201 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1261 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1321 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1381 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1441 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1501 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1561 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1621 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1681 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
     1741 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1801 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1861 tgcaataaac aagttagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     1921 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     1981 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggac tatggttgct
     2041 gactaattga gatgcggatc cgctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2101 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2161 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2221 gctatgacca tgattacgcc aagcttgggc tgcaggtcga gggatctcca taagagaaga
     2281 gggacagcta tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt
     2341 aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc
     2401 taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata
     2461 tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt
     2521 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
     2581 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
     2641 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
     2701 ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc caccaccttc
     2761 tgataggcag cctgcacctg aggagtgcgg ccgctttact tgtacagctc gtccatgccg
     2821 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     2881 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
     2941 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
     3001 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
     3061 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
     3121 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
     3181 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
     3241 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
     3301 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
     3361 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
     3421 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
     3481 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc
     3541 cgcggtaccg tcgactgcag aattcaattt aaatcgtacc gagcgactcg agataacttc
     3601 gtataatgta tgctatacga agttattcgg atttgatcca gacatgataa gatacattga
     3661 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3721 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta tcgatggggt
     3781 gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga
     3841 aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg
     3901 gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc
     3961 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     4021 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     4081 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     4141 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     4201 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     4261 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     4321 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     4381 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     4441 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     4501 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     4561 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     4621 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     4681 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     4741 catgcgaaac gatcctcatc ctgtctcttg atcagatcaa atccgataac ttcgtataat
     4801 gtatgctata cgaagttatc agaatatgtg gtgaagttca ctctacagag aagttcctat
     4861 actttctaga gaataggaac ttctcggatt tgatccagac atgataagat acattgatga
     4921 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4981 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttatcg atggggtggg
     5041 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     5101 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     5161 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     5221 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     5281 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     5341 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     5401 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     5461 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     5521 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     5581 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     5641 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     5701 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     5761 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     5821 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     5881 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     5941 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     6001 gcgaaacgat cctcatcctg tctcttgatc agatcaaatc cggaagttcc tatactttct
     6061 agagaatagg aacttcacgc gttcgctcga attaatcaat tctttgccaa aatgatgaga
     6121 cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg catgaacatg
     6181 gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg ccctgccccg
     6241 tcccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag ggagaagggg
     6301 acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc cggcgccgca
     6361 ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc ctcccagatt tcggctccgc
     6421 acagatttgg gacaaaggaa gtccctgcgc cctctcgcac gattaccata aaaggcaatg
     6481 gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg gggccgccgc gcccctcccc
     6541 cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct gccgccaccc
     6601 cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg agccccgcac ccgaagccgg
     6661 gccgtgctca gcaactcggg gaggggggtg cagggggggt tgcagcccga ccgacgcgcc
     6721 cacaccccct gctcaccccc ccacgcacac accccgcacg cagcctttgt tcccctcgca
     6781 gccccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca cactgcggag
     6841 cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg ccgggcagcg cggagccgca
     6901 cgcggcgctc cccacgcaca cacacacgca cgcacccccc gagccgctcc ccccgcacaa
     6961 agggccctcc cggagcccct caaggctttc acgcagccac agaaaagaaa caagccgtca
     7021 ttaaaccaag cgctaattac agcccggagg agaagggccg tcccgcccgc tcacctgtgg
     7081 gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg agcggggcac
     7141 ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt tatagggccg
     7201 ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct gattggctgc
     7261 cgccgcacct ctccgcctcg ccccgccccg cccctcgccc cgccccgccc cgcctggcgc
     7321 gcgccccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat aaataaatac
     7381 aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt ggggctcacc
     7441 tcgaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     7501 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     7561 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     7621 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     7681 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     7741 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     7801 taataactag tcaataatca atgtcgaccc aggtggcact tttcggggaa atgtgcgcgg
     7861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7981 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8041 gctggtgaaa gtaaaagatg ctgaagatca gttgggtg
//
