LOCUS       plasmid                 7032 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     enhancer        395..774
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    4001..4225
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    5299..5433
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(260..364)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          1053..2069
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             2204..3325
                     /note="pLannotate"
                     /label="FLPo (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="86.4"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(join(6431..7032,1..259))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(5672..6260)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    4530..4585
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4994..5024)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3368..3970
                     /note="pLannotate"
                     /label="FHY1"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="99.5"
                     /fragment="False"
                     /other="CDS"
     promoter        776..1052
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5039..5060)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(4970..4986)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      5145..5279
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             4388..4459
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5435..5489)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        5119..5141
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(5073..5112)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
     promoter        4229..4250
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
      421 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      481 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      541 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      601 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      661 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      721 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
      781 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
      841 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggcgcg
      901 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
      961 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     1021 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg
     1081 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     1141 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     1201 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     1261 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     1321 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1381 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     1441 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     1501 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1561 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1621 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     1681 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     1741 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     1801 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     1861 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     1921 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     1981 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     2041 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     2101 ctcatcattt tggcaaagaa ttgattaatt cgagcgaagg cgcgcccatt actcgcatcc
     2161 attctcaggc tgtctcgtct cgtctcgcac gcgtgccgcc accatgagcc agttcgacat
     2221 cctgtgcaag acccccccca aggtgctggt gcggcagttc gtggagagat tcgagaggcc
     2281 cagcggcgag aagatcgcca gctgtgccgc cgagctgacc tacctgtgct ggatgatcac
     2341 ccacaacggc accgccatca agagggccac cttcatgagc tacaacacca tcatcagcaa
     2401 cagcctgagc ttcgacatcg tgaacaagag cctgcagttc aagtacaaga cccagaaggc
     2461 caccatcctg gaggccagcc tgaagaagct gatccccgcc tgggagttca ccatcatccc
     2521 ttacaacggc cagaagcacc agagcgacat caccgacatc gtgtccagcc tgcagctgca
     2581 gttcgagagc agcgaggagg ccgacaaggg caacagccac agcaagaaga tgctgaaggc
     2641 cctgctgtcc gagggcgaga gcatctggga gatcaccgag aagatcctga acagcttcga
     2701 gtacaccagc aggttcacca agaccaagac cctgtaccag ttcctgttcc tggccacatt
     2761 catcaactgc ggcaggttca gcgacatcaa gaacgtggac cccaagagct tcaagctggt
     2821 gcagaacaag tacctgggcg tgatcattca gtgcctggtg accgagacca agacaagcgt
     2881 gtccaggcac atctactttt tcagcgccag aggcaggatc gaccccctgg tgtacctgga
     2941 cgagttcctg aggaacagcg agcccgtgct gaagagagtg aacaggaccg gcaacagcag
     3001 cagcaacaag caggagtacc agctgctgaa ggacaacctg gtgcgcagct acaacaaggc
     3061 cctgaagaag aacgccccct accccatctt cgctatcaag aacggcccta agagccacat
     3121 cggcaggcac ctgatgacca gctttctgag catgaagggc ctgaccgagc tgacaaacgt
     3181 ggtgggcaac tggagcgaca agagggcctc cgccgtggcc aggaccacct acacccacca
     3241 gatcaccgcc atccccgacc actacttcgc cctggtgtcc aggtactacg cctacgaccc
     3301 catcagcaag gagatgatcg ccctgtccgg agggtctggc tccggatcaa gtggtggcag
     3361 cggtaccccc gaggtggagg tcgacaacaa caacgagaag cccagcgaga tcaacagctt
     3421 ccaccacatg attatcagca gcagcaagaa cgtgctgaag atggaggagg tggaggtgtc
     3481 caagaagagg aagttccaga ccgaccagag cgacgagctg agcttattac ctttatccaa
     3541 acacacatgc tttgccaacg tggcttgtag cgagaacacc aacggcaaca gcgagatcga
     3601 taccgagtac agcatgtcca gctacgtgaa ctccaccacc tccatggagt gcaacaacga
     3661 catcgagatg aaggaagaat ccagcggcag ctgcggagag gacaagatga tcagcttcga
     3721 gtcccattta gactatatct acggcaccca gaatctggag gacttctccg agaaggtgat
     3781 cgagaacatt ctgtacctcg acgagcaaga agaggaggag gaggacgcca agggctgtag
     3841 cagcaacgct gccaagttcg tgctgtcctc tggtcgttgg accgtgaacc aagatgatag
     3901 cactttacac gaaaccaaga agcccaccat cgaccaagaa ttcgagcagt actttagcac
     3961 tttaatgctg cccaagaaaa agcggaaggt gtgagaattc ctgtgccttc tagttgccag
     4021 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4081 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4141 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4201 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4261 gggtatcccc acgcgccctg tagcgtgtac cgctgggagt tcgtagacgg aaacaaacgc
     4321 agaatccaag cgcggccgcc caggatacat agattaccac aactccgagc ccttccaccg
     4381 ctagcgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     4441 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     4501 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     4561 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     4621 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     4681 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     4741 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     4801 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     4861 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     4921 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4981 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5041 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5101 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5161 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5221 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5281 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5341 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5401 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     5461 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5521 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5581 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5641 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5701 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5761 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5821 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5881 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5941 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6001 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6061 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6121 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6181 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6241 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6301 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6361 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6421 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6481 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6541 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6601 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6721 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6781 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6841 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6901 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6961 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7021 gaccgagttg ct
//
