LOCUS       plasmid                 7238 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     enhancer        4266..4645
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    840..1064
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    2138..2272
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4131..4235)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          4923..5939
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(3270..4130)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2511..3099)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    1369..1424
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             join(6086..7238,1..329)
                     /note="pLannotate"
                     /label="PHYA5"
                     /database="swissprot"
                     /identity="69.2"
                     /match_length="99.8"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(1833..1863)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4647..4922
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1878..1899)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(1809..1825)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      1984..2118
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1227..1298
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             728..809
                     /note="pLannotate"
                     /label="FLPo (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2274..2328)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        1958..1980
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(1912..1951)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
     promoter        1068..1089
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 atgcttgtga atttttagcc caagtgtttg ccatccacgt caacaaggag gtcgagctgg
       61 acaaccagat ggtggagaag aacattttac gtacccagac actcctctgc gacatgctca
      121 tgagggacgc tcccctcggc atcgtgagcc agtcccccaa tattatggat ttagtcaagt
      181 gcgacggcgc cgccttatta tacaaggaca agatctggaa gctgggcacc acccctagcg
      241 agttccattt acaagaaatc gcttcttggc tgtgtgagta ccacatggat tccaccggtt
      301 taagcaccga ttctttacac gacgctggct ttcctagggc tttatcttta ggcgacagcg
      361 tctgcggaat ggctgccgtt cgtatcagca gcaaagatat gattttctgg tttcgttccc
      421 ataccgccgg cgaagtgaga tggggcggcg ccaaacacga ccccgatgat agggacgatg
      481 ccagaaggat gcaccccaga tcctccttca aggcttttct ggaggtggtg aagaccagaa
      541 gcttaccttg gaaggactac gaaatggatg ccatccactc tttacagctc atcttacgta
      601 acgcctttaa ggacagcgag accaccgacg tgaacacaaa ggtgatctat agcaagctca
      661 acgatctcaa gattgatgga atccaatccg gagggtctgg ctccggatca agtggtggca
      721 gcggtaccag catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact
      781 acctgagcag ctacatcaac aggcggatcc ccaagaaaaa gcggaaggtg tgagaattcc
      841 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      901 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
      961 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1021 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     1081 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcgtgtacc gctgggagtt
     1141 cgtagacgga aacaaacgca gaatccaagc gcggccgccc aggatacata gattaccaca
     1201 actccgagcc cttccaccgc tagcgcactc ctcaggtgca ggctgcctat cagaaggtgg
     1261 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     1321 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     1381 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     1441 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     1501 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     1561 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     1621 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     1681 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     1741 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     1801 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1861 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1921 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     1981 tagtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2041 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     2101 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     2161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     2281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2341 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2401 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2461 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2521 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2581 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2641 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2701 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2761 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2821 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2881 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2941 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3001 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3061 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3121 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3181 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3241 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3301 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3361 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3421 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3481 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3541 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3601 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3661 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3721 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3781 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3841 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3901 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3961 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4021 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4081 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4141 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4201 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4261 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4321 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4381 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4441 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4501 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4561 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4621 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     4681 catctccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     4741 gcgatggggg cggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
     4801 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     4861 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     4921 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     4981 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     5041 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     5101 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     5161 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     5221 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     5281 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     5341 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     5401 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     5461 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     5521 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     5581 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     5641 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     5701 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     5761 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     5821 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     5881 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     5941 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgattaatt
     6001 cgagcgaagg cgcgcccatt actcgcatcc attctcaggc tgtctcgtct cgtctcgcac
     6061 gcgtgccgcc accatggaga agaagatgag cggatctcgt cccacacagt ccagcgaggg
     6121 atctcgtaga tctcgtcact ccgctcgtat catcgctcag accaccgtgg acgccaaact
     6181 gcacgccgat ttcgaggaga gcggctcctc ctttgattac tccaccagcg tgagggtgac
     6241 tggtcccgtg gtcgagaacc agcctcctag gagcgacaag gtcaccacaa cctacctcca
     6301 tcatatccag aagggcaagc tgatccagcc ctttggatgt ttactggctt tagacgaaaa
     6361 gaccttcaag gtcatcgcct actccgagaa cgcctccgaa ctgctcacaa tggcttccca
     6421 cgccgtgcct agcgtgggag agcaccccgt tctgggcatc ggcaccgata tcagatcttt
     6481 atttaccgct cccagcgctt ccgctttaca gaaggctctc ggcttcggcg acgtgtcttt
     6541 actgaaccct atcctcgtcc attgtcgtac atccgccaag cccttctacg ccattatcca
     6601 tagggtgacc ggatccatca tcatcgactt cgaacccgtt aagccctatg aagtgcccat
     6661 gacagccgcc ggagctttac agagctataa gctggccgcc aaggccatca ccagactcca
     6721 gtctttaccc agcggaagca tggagaggct gtgcgacacc atggtgcaag aagtcttcga
     6781 gctgactggt tacgatcgtg tcatggccta caagttccac gaggacgacc acggcgaggt
     6841 ggtgagcgaa gtgaccaagc ccggtttaga accctattta ggtttacact atcccgccac
     6901 agacatcccc caagctgcca gatttttatt tatgaagaac aaggtgagaa tgatcgtgga
     6961 ctgcaacgcc aagcacgcta gggttttaca agatgagaag ctgagcttcg atttaaccct
     7021 ctgcggcagc actttaagag ctccccactc ttgtcatctg caatacatgg ccaatatgga
     7081 cagcatcgct tctttagtga tggccgtggt ggtgaatgag gaggatggag agggcgatgc
     7141 tcccgatgcc accacacagc ctcagaagag aaagaggctg tggggtttag tggtctgcca
     7201 caacaccacc cccagatttg tcccttttcc cttacgtt
//
