LOCUS       plasmid                 6254 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     enhancer        384..763
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    3212..3436
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             2192..2797
                     /note="pLannotate"
                     /label="FHY1"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    4510..4644
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(249..353)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          1041..2057
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(join(5642..6254,1..248))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4883..5471)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    3741..3796
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4205..4235)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        765..1040
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4250..4271)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(4181..4197)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      4356..4490
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2840..3181
                     /note="pLannotate"
                     /label="cre (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="33.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3599..3670
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4646..4700)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        4330..4352
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(4284..4323)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
     promoter        3440..3461
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
       61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      361 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
      421 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      481 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      541 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      601 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      661 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      721 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
      781 gttctgcttc actctcccca tctccccccc tccccacccc caattttgta tttatttatt
      841 ttttaattat tttgtgcagc gatgggggcg gggggggggg ggggcgcgcg ccaggcgggg
      901 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
      961 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     1021 agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg
     1081 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     1141 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt
     1201 cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga
     1261 gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc
     1321 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc
     1381 gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca
     1441 aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg
     1501 ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg
     1561 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
     1621 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
     1681 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     1741 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     1801 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     1861 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     1921 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     1981 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     2041 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     2101 gcaaagaatt gattaattcg agcgaaggcg cgcccattac tcgcatccat tctcaggctg
     2161 tctcgtctcg tctcgcacgc gtgccgccac catgcccgag gtggaggtcg acaacaacaa
     2221 cgagaagccc agcgagatca acagcttcca ccacatgatt atcagcagca gcaagaacgt
     2281 gctgaagatg gaggaggtgg aggtgtccaa gaagaggaag ttccagaccg accagagcga
     2341 cgagctgagc ttattacctt tatccaaaca cacatgcttt gccaacgtgg cttgtagcga
     2401 gaacaccaac ggcaacagcg agatcgatac cgagtacagc atgtccagct acgtgaactc
     2461 caccacctcc atggagtgca acaacgacat cgagatgaag gaagaatcca gcggcagctg
     2521 cggagaggac aagatgatca gcttcgagtc ccatttagac tatatctacg gcacccagaa
     2581 tctggaggac ttctccgaga aggtgatcga gaacattctg tacctcgacg agcaagaaga
     2641 ggaggaggag gacgccaagg gctgtagcag caacgctgcc aagttcgtgc tgtcctctgg
     2701 tcgttggacc gtgaaccaag atgatagcac tttacacgaa accaagaagc ccaccatcga
     2761 ccaagaattc gagcagtact ttagcacttt aatgctgtcc ggagggtctg gctccggatc
     2821 aagtggtggc agcggtaccg tggctgatga ccccaacaac tacctgttct gccgggtcag
     2881 aaagaatggt gtggctgccc cttctgccac ctcccaactg tccacccggg ccctggaagg
     2941 gatctttgag gccacccacc gcctgatcta tggtgccaag gatgactctg ggcagagata
     3001 cctggcctgg tctggccact ctgccagagt gggtgctgcc agggacatgg ccagggctgg
     3061 tgtgtccatc cctgaaatca tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa
     3121 ctacatcaga aacctggact ctgagactgg ggccatggtg aggctgctcg aggatgggga
     3181 ccccaagaaa aagcggaagg tgtgagaatt cctgtgcctt ctagttgcca gccatctgtt
     3241 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3301 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3361 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3421 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     3481 cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg gaaacaaacg cagaatccaa
     3541 gcgcggccgc ccaggataca tagattacca caactccgag cccttccacc gctagcgcac
     3601 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     3661 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     3721 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     3781 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     3841 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     3901 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     3961 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     4021 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     4081 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     4141 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4201 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4261 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4321 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     4381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4441 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4501 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4621 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     4681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6241 gctcttgccc ggcg
//
