LOCUS       plasmid                 6189 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     enhancer        394..773
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    3157..3381
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             2203..2808
                     /note="pLannotate"
                     /label="FHY1"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    4455..4589
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(259..363)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          1052..2068
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(join(5587..6189,1..258))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4828..5416)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    3686..3741
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4150..4180)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        775..1051
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4195..4216)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(4126..4142)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      4301..4435
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2848..3126
                     /note="pLannotate"
                     /label="cre (fragment)"
                     /database="swissprot"
                     /identity="98.9"
                     /match_length="27.1"
                     /fragment="True"
                     /other="CDS"
     CDS             3544..3615
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4591..4645)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        4275..4297
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(4229..4268)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
     promoter        3385..3406
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
       61 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      361 gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat
      421 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      481 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      541 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      601 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      661 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      721 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
      781 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
      841 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg gggggcgcgc
      901 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
      961 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     1021 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     1081 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     1141 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
     1201 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
     1261 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     1321 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
     1381 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
     1441 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     1501 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
     1561 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     1621 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     1681 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     1741 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     1801 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     1861 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     1921 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     1981 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     2041 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     2101 tcatcatttt ggcaaagaat tgattaattc gagcgaaggc gcgcccatta ctcgcatcca
     2161 ttctcaggct gtctcgtctc gtctcgcacg cgtgccgcca ccatgcccga ggtggaggtc
     2221 gacaacaaca acgagaagcc cagcgagatc aacagcttcc accacatgat tatcagcagc
     2281 agcaagaacg tgctgaagat ggaggaggtg gaggtgtcca agaagaggaa gttccagacc
     2341 gaccagagcg acgagctgag cttattacct ttatccaaac acacatgctt tgccaacgtg
     2401 gcttgtagcg agaacaccaa cggcaacagc gagatcgata ccgagtacag catgtccagc
     2461 tacgtgaact ccaccacctc catggagtgc aacaacgaca tcgagatgaa ggaagaatcc
     2521 agcggcagct gcggagagga caagatgatc agcttcgagt cccatttaga ctatatctac
     2581 ggcacccaga atctggagga cttctccgag aaggtgatcg agaacattct gtacctcgac
     2641 gagcaagaag aggaggagga ggacgccaag ggctgtagca gcaacgctgc caagttcgtg
     2701 ctgtcctctg gtcgttggac cgtgaaccaa gatgatagca ctttacacga aaccaagaag
     2761 cccaccatcg accaagaatt cgagcagtac tttagcactt taatgctgtc cggagggtct
     2821 ggctccggat caagtggtgg cagcggtacc gccacctccc aactgtccac ccgggccctg
     2881 gaagggatct ttgaggccac ccaccgcctg atctatggtg ccaaggatga ctctgggcag
     2941 agatacctgg cctggtctgg ccactctgcc agagtgggtg ctgccaggga catggccagg
     3001 gctggtgtgt ccatccctga aatcatgcag gctggtggct ggaccaatgt gaacattgtg
     3061 atgaactaca tcagaaacct ggactctgag actggggcca tggtgaggct gctcgaggat
     3121 ggggacccca agaaaaagcg gaaggtgtga gaattcctgt gccttctagt tgccagccat
     3181 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3241 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3301 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3361 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     3421 atccccacgc gccctgtagc gtgtaccgct gggagttcgt agacggaaac aaacgcagaa
     3481 tccaagcgcg gccgcccagg atacatagat taccacaact ccgagccctt ccaccgctag
     3541 cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     3601 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     3661 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     3721 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     3781 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     3841 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     3901 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     3961 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     4021 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     4081 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4261 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4321 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4381 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4441 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4561 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     4621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6181 gagttgctc
//
