LOCUS       plasmid                 7435 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     enhancer        complement(6809..7188)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(6530..6807)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(2955..3179)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(1747..1881)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        7219..7323
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          complement(5513..6529)
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             join(7324..7435,1..749)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      920..1508
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(2595..2650)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(3885..5366)
                     /note="pLannotate"
                     /label="PHYA5"
                     /database="swissprot"
                     /identity="69.2"
                     /match_length="99.8"
                     /fragment="False"
                     /other="CDS"
     promoter        2156..2186
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    2120..2141
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    2194..2210
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(1901..2035)
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3210..3488)
                     /note="pLannotate"
                     /label="cre (fragment)"
                     /database="swissprot"
                     /identity="98.9"
                     /match_length="27.1"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2721..2792)
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             1691..1745
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2039..2061)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             2068..2107
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2930..2951)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1741 gcagcggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1801 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1861 ataagctgca ataaacaagt tagctttttg caaaagccta ggcctccaaa aaagcctcct
     1921 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     1981 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggactatg
     2041 gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac tggaaagcgg
     2101 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2161 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2221 gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga tctccataag
     2281 agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct ggctagtaaa
     2341 acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa
     2401 aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc agggggctgt
     2461 ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag catatgggca
     2521 tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt tgccctccca
     2581 tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat gaaaataaat
     2641 ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc ataatttttg
     2701 gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac accagccacc
     2761 accttctgat aggcagcctg cacctgagga gtgcgctagc ggtggaaggg ctcggagttg
     2821 tggtaatcta tgtatcctgg gcggccgcgc ttggattctg cgtttgtttc cgtctacgaa
     2881 ctcccagcgg tacacgctac agggcgcgtg gggatacccc ctagagcccc agctggttct
     2941 ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     3001 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     3061 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     3121 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagg
     3181 aattctcaca ccttccgctt tttcttgggg tccccatcct cgagcagcct caccatggcc
     3241 ccagtctcag agtccaggtt tctgatgtag ttcatcacaa tgttcacatt ggtccagcca
     3301 ccagcctgca tgatttcagg gatggacaca ccagccctgg ccatgtccct ggcagcaccc
     3361 actctggcag agtggccaga ccaggccagg tatctctgcc cagagtcatc cttggcacca
     3421 tagatcaggc ggtgggtggc ctcaaagatc ccttccaggg cccgggtgga cagttgggag
     3481 gtggcggtac cgctgccacc acttgatccg gagccagacc ctccggattg gattccatca
     3541 atcttgagat cgttgagctt gctatagatc acctttgtgt tcacgtcggt ggtctcgctg
     3601 tccttaaagg cgttacgtaa gatgagctgt aaagagtgga tggcatccat ttcgtagtcc
     3661 ttccaaggta agcttctggt cttcaccacc tccagaaaag ccttgaagga ggatctgggg
     3721 tgcatccttc tggcatcgtc cctatcatcg gggtcgtgtt tggcgccgcc ccatctcact
     3781 tcgccggcgg tatgggaacg aaaccagaaa atcatatctt tgctgctgat acgaacggca
     3841 gccattccgc agacgctgtc gcctaaagat aaagccctag gaaagccagc gtcgtgtaaa
     3901 gaatcggtgc ttaaaccggt ggaatccatg tggtactcac acagccaaga agcgatttct
     3961 tgtaaatgga actcgctagg ggtggtgccc agcttccaga tcttgtcctt gtataataag
     4021 gcggcgccgt cgcacttgac taaatccata atattggggg actggctcac gatgccgagg
     4081 ggagcgtccc tcatgagcat gtcgcagagg agtgtctggg tacgtaaaat gttcttctcc
     4141 accatctggt tgtccagctc gacctccttg ttgacgtgga tggcaaacac ttgggctaaa
     4201 aattcacaag cataacgtaa gggaaaaggg acaaatctgg gggtggtgtt gtggcagacc
     4261 actaaacccc acagcctctt tctcttctga ggctgtgtgg tggcatcggg agcatcgccc
     4321 tctccatcct cctcattcac caccacggcc atcactaaag aagcgatgct gtccatattg
     4381 gccatgtatt gcagatgaca agagtgggga gctcttaaag tgctgccgca gagggttaaa
     4441 tcgaagctca gcttctcatc ttgtaaaacc ctagcgtgct tggcgttgca gtccacgatc
     4501 attctcacct tgttcttcat aaataaaaat ctggcagctt gggggatgtc tgtggcggga
     4561 tagtgtaaac ctaaataggg ttctaaaccg ggcttggtca cttcgctcac cacctcgccg
     4621 tggtcgtcct cgtggaactt gtaggccatg acacgatcgt aaccagtcag ctcgaagact
     4681 tcttgcacca tggtgtcgca cagcctctcc atgcttccgc tgggtaaaga ctggagtctg
     4741 gtgatggcct tggcggccag cttatagctc tgtaaagctc cggcggctgt catgggcact
     4801 tcatagggct taacgggttc gaagtcgatg atgatggatc cggtcaccct atggataatg
     4861 gcgtagaagg gcttggcgga tgtacgacaa tggacgagga tagggttcag taaagacacg
     4921 tcgccgaagc cgagagcctt ctgtaaagcg gaagcgctgg gagcggtaaa taaagatctg
     4981 atatcggtgc cgatgcccag aacggggtgc tctcccacgc taggcacggc gtgggaagcc
     5041 attgtgagca gttcggaggc gttctcggag taggcgatga ccttgaaggt cttttcgtct
     5101 aaagccagta aacatccaaa gggctggatc agcttgccct tctggatatg atggaggtag
     5161 gttgtggtga ccttgtcgct cctaggaggc tggttctcga ccacgggacc agtcaccctc
     5221 acgctggtgg agtaatcaaa ggaggagccg ctctcctcga aatcggcgtg cagtttggcg
     5281 tccacggtgg tctgagcgat gatacgagcg gagtgacgag atctacgaga tccctcgctg
     5341 gactgtgtgg gacgagatcc gctcatcttc ttctccatgg tggcggcacg cgtgcgagac
     5401 gagacgagac agcctgagaa tggatgcgag taatgggcgc gccttcgctc gaattaatca
     5461 attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga
     5521 aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga
     5581 agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc
     5641 gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca
     5701 tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc
     5761 gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc
     5821 acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc
     5881 gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc gccccgcccg
     5941 gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgtac
     6001 ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg
     6061 gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac acaccccgca
     6121 cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc
     6181 tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc
     6241 cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc
     6301 ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc
     6361 acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc
     6421 cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg
     6481 aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg
     6541 cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga
     6601 gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc
     6661 cccgccccgc cccgcctggc gcgcgccccc cccccccccc cgcccccatc gctgcacaaa
     6721 ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag atggggagag
     6781 tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat acgtagatgt
     6841 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     6901 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     6961 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     7021 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     7081 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     7141 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga cccaggtggc
     7201 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7261 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7321 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7381 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagt
//
