LOCUS       plasmid                 7500 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     enhancer        complement(6879..7258)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    complement(2960..3184)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(1752..1886)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        7289..7393
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          complement(5584..6600)
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             join(7394..7500,1..754)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      925..1513
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(2600..2655)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(3956..5437)
                     /note="pLannotate"
                     /label="PHYA5"
                     /database="swissprot"
                     /identity="69.2"
                     /match_length="99.8"
                     /fragment="False"
                     /other="CDS"
     promoter        2161..2191
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(6601..6877)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    2125..2146
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    2199..2215
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(1906..2040)
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3215..3556)
                     /note="pLannotate"
                     /label="cre (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="33.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2726..2797)
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             1696..1750
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2044..2066)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             2073..2112
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2935..2956)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
       61 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
      841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1621 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1681 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1741 ttaatgcagc ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     1801 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     1861 ccattataag ctgcaataaa caagttagct ttttgcaaaa gcctaggcct ccaaaaaagc
     1921 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     1981 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     2041 ctatggttgc tgactaattg agatgcggat ccgctggcac gacaggtttc ccgactggaa
     2101 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2161 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2221 cacaggaaac agctatgacc atgattacgc caagcttggg ctgcaggtcg agggatctcc
     2281 ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa aatctggcta
     2341 gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata
     2401 taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg acagcagggg
     2461 gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca gccagcatat
     2521 gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     2581 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     2641 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
     2701 ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg gccacaccag
     2761 ccaccacctt ctgataggca gcctgcacct gaggagtgcg ctagcggtgg aagggctcgg
     2821 agttgtggta atctatgtat cctgggcggc cgcgcttgga ttctgcgttt gtttccgtct
     2881 acgaactccc agcggtacac gctacagggc gcgtggggat accccctaga gccccagctg
     2941 gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct
     3001 tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta
     3061 ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct
     3121 tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc
     3181 acaggaattc tcacaccttc cgctttttct tggggtcccc atcctcgagc agcctcacca
     3241 tggccccagt ctcagagtcc aggtttctga tgtagttcat cacaatgttc acattggtcc
     3301 agccaccagc ctgcatgatt tcagggatgg acacaccagc cctggccatg tccctggcag
     3361 cacccactct ggcagagtgg ccagaccagg ccaggtatct ctgcccagag tcatccttgg
     3421 caccatagat caggcggtgg gtggcctcaa agatcccttc cagggcccgg gtggacagtt
     3481 gggaggtggc agaaggggca gccacaccat tctttctgac ccggcagaac aggtagttgt
     3541 tggggtcatc agccacggta ccgctgccac cacttgatcc ggagccagac cctccggatt
     3601 ggattccatc aatcttgaga tcgttgagct tgctatagat cacctttgtg ttcacgtcgg
     3661 tggtctcgct gtccttaaag gcgttacgta agatgagctg taaagagtgg atggcatcca
     3721 tttcgtagtc cttccaaggt aagcttctgg tcttcaccac ctccagaaaa gccttgaagg
     3781 aggatctggg gtgcatcctt ctggcatcgt ccctatcatc ggggtcgtgt ttggcgccgc
     3841 cccatctcac ttcgccggcg gtatgggaac gaaaccagaa aatcatatct ttgctgctga
     3901 tacgaacggc agccattccg cagacgctgt cgcctaaaga taaagcccta ggaaagccag
     3961 cgtcgtgtaa agaatcggtg cttaaaccgg tggaatccat gtggtactca cacagccaag
     4021 aagcgatttc ttgtaaatgg aactcgctag gggtggtgcc cagcttccag atcttgtcct
     4081 tgtataataa ggcggcgccg tcgcacttga ctaaatccat aatattgggg gactggctca
     4141 cgatgccgag gggagcgtcc ctcatgagca tgtcgcagag gagtgtctgg gtacgtaaaa
     4201 tgttcttctc caccatctgg ttgtccagct cgacctcctt gttgacgtgg atggcaaaca
     4261 cttgggctaa aaattcacaa gcataacgta agggaaaagg gacaaatctg ggggtggtgt
     4321 tgtggcagac cactaaaccc cacagcctct ttctcttctg aggctgtgtg gtggcatcgg
     4381 gagcatcgcc ctctccatcc tcctcattca ccaccacggc catcactaaa gaagcgatgc
     4441 tgtccatatt ggccatgtat tgcagatgac aagagtgggg agctcttaaa gtgctgccgc
     4501 agagggttaa atcgaagctc agcttctcat cttgtaaaac cctagcgtgc ttggcgttgc
     4561 agtccacgat cattctcacc ttgttcttca taaataaaaa tctggcagct tgggggatgt
     4621 ctgtggcggg atagtgtaaa cctaaatagg gttctaaacc gggcttggtc acttcgctca
     4681 ccacctcgcc gtggtcgtcc tcgtggaact tgtaggccat gacacgatcg taaccagtca
     4741 gctcgaagac ttcttgcacc atggtgtcgc acagcctctc catgcttccg ctgggtaaag
     4801 actggagtct ggtgatggcc ttggcggcca gcttatagct ctgtaaagct ccggcggctg
     4861 tcatgggcac ttcatagggc ttaacgggtt cgaagtcgat gatgatggat ccggtcaccc
     4921 tatggataat ggcgtagaag ggcttggcgg atgtacgaca atggacgagg atagggttca
     4981 gtaaagacac gtcgccgaag ccgagagcct tctgtaaagc ggaagcgctg ggagcggtaa
     5041 ataaagatct gatatcggtg ccgatgccca gaacggggtg ctctcccacg ctaggcacgg
     5101 cgtgggaagc cattgtgagc agttcggagg cgttctcgga gtaggcgatg accttgaagg
     5161 tcttttcgtc taaagccagt aaacatccaa agggctggat cagcttgccc ttctggatat
     5221 gatggaggta ggttgtggtg accttgtcgc tcctaggagg ctggttctcg accacgggac
     5281 cagtcaccct cacgctggtg gagtaatcaa aggaggagcc gctctcctcg aaatcggcgt
     5341 gcagtttggc gtccacggtg gtctgagcga tgatacgagc ggagtgacga gatctacgag
     5401 atccctcgct ggactgtgtg ggacgagatc cgctcatctt cttctccatg gtggcggcac
     5461 gcgtgcgaga cgagacgaga cagcctgaga atggatgcga gtaatgggcg cgccttcgct
     5521 cgaattaatc aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag
     5581 gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggctctag agccgccggt
     5641 cacacgccag aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc cgtccctccg
     5701 cggacagccc cgaggctgga gagggagaag gggacggcgg cgcggcgacg cacgaaggcc
     5761 ctccccgccc atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg
     5821 ggtgcggcgg cgcctcccag atttcggctc cgcacagatt tgggacaaag gaagtccctg
     5881 cgccctctcg cacgattacc ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc
     5941 gccggcgctc cgggggccgc cgcgcccctc ccccgagccc tccccggccc gaggcggccc
     6001 cgccccgccc ggcaccccca cctgccgcca ccccccgccc ggcacggcga gccccgcgcc
     6061 acgccccgta cggagccccg cacccgaagc cgggccgtgc tcagcaactc ggggaggggg
     6121 gtgcaggggg ggttgcagcc cgaccgacgc gcccacaccc cctgctcacc cccccacgca
     6181 cacaccccgc acgcagcctt tgttcccctc gcagcccccc ccgcaccgcg gggcaccgcc
     6241 cccggccgcg ctcccctcgc gcacactgcg gagcgcacaa agccccgcgc cgcgcccgca
     6301 gcgctcacag ccgccgggca gcgcggagcc gcacgcggcg ctccccacgc acacacacac
     6361 gcacgcaccc cccgagccgc tccccccgca caaagggccc tcccggagcc cctcaaggct
     6421 ttcacgcagc cacagaaaag aaacaagccg tcattaaacc aagcgctaat tacagcccgg
     6481 aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc agagccgggg
     6541 cgggcggcgc gaggcggcgg cggagcgggg cacggggcga aggcagcgcg cagcgactcc
     6601 cgcccgccgc gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg ccataaaagg
     6661 aaactttcgg agcgcgccgc tctgattggc tgccgccgca cctctccgcc tcgccccgcc
     6721 ccgcccctcg ccccgccccg ccccgcctgg cgcgcgcccc cccccccccc cgcccccatc
     6781 gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag
     6841 atggggagag tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat
     6901 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     6961 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     7021 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     7081 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     7141 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     7201 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga
     7261 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
//
