LOCUS       plasmid                 7467 bp    DNA     circular SYN 17-JAN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     enhancer        811..1190
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    4852..5076
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    6150..6284
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(676..780)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          1469..2485
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(join(7282..7467,1..675))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(6523..7111)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    5381..5436
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             2632..4113
                     /note="pLannotate"
                     /label="PHYA5"
                     /database="swissprot"
                     /identity="69.2"
                     /match_length="99.8"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(5845..5875)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1192..1468
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5890..5911)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(5821..5837)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      5996..6130
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="False"
                     /other="rep_origin"
     CDS             5239..5310
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6286..6340)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        5970..5992
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             complement(5924..5963)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
     promoter        5080..5101
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
       61 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      121 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      661 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      781 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
      841 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      901 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      961 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1021 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1081 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1141 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     1201 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     1261 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg ggcgcgcgcc
     1321 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     1381 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1441 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1501 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1561 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     1621 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1681 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     1741 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1801 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     1861 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1921 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     1981 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     2041 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     2101 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2161 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     2221 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     2281 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     2341 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     2401 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     2461 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     2521 tcattttggc aaagaattga ttaattcgag cgaaggcgcg cccattactc gcatccattc
     2581 tcaggctgtc tcgtctcgtc tcgcacgcgt gccgccacca tggagaagaa gatgagcgga
     2641 tctcgtccca cacagtccag cgagggatct cgtagatctc gtcactccgc tcgtatcatc
     2701 gctcagacca ccgtggacgc caaactgcac gccgatttcg aggagagcgg ctcctccttt
     2761 gattactcca ccagcgtgag ggtgactggt cccgtggtcg agaaccagcc tcctaggagc
     2821 gacaaggtca ccacaaccta cctccatcat atccagaagg gcaagctgat ccagcccttt
     2881 ggatgtttac tggctttaga cgaaaagacc ttcaaggtca tcgcctactc cgagaacgcc
     2941 tccgaactgc tcacaatggc ttcccacgcc gtgcctagcg tgggagagca ccccgttctg
     3001 ggcatcggca ccgatatcag atctttattt accgctccca gcgcttccgc tttacagaag
     3061 gctctcggct tcggcgacgt gtctttactg aaccctatcc tcgtccattg tcgtacatcc
     3121 gccaagccct tctacgccat tatccatagg gtgaccggat ccatcatcat cgacttcgaa
     3181 cccgttaagc cctatgaagt gcccatgaca gccgccggag ctttacagag ctataagctg
     3241 gccgccaagg ccatcaccag actccagtct ttacccagcg gaagcatgga gaggctgtgc
     3301 gacaccatgg tgcaagaagt cttcgagctg actggttacg atcgtgtcat ggcctacaag
     3361 ttccacgagg acgaccacgg cgaggtggtg agcgaagtga ccaagcccgg tttagaaccc
     3421 tatttaggtt tacactatcc cgccacagac atcccccaag ctgccagatt tttatttatg
     3481 aagaacaagg tgagaatgat cgtggactgc aacgccaagc acgctagggt tttacaagat
     3541 gagaagctga gcttcgattt aaccctctgc ggcagcactt taagagctcc ccactcttgt
     3601 catctgcaat acatggccaa tatggacagc atcgcttctt tagtgatggc cgtggtggtg
     3661 aatgaggagg atggagaggg cgatgctccc gatgccacca cacagcctca gaagagaaag
     3721 aggctgtggg gtttagtggt ctgccacaac accaccccca gatttgtccc ttttccctta
     3781 cgttatgctt gtgaattttt agcccaagtg tttgccatcc acgtcaacaa ggaggtcgag
     3841 ctggacaacc agatggtgga gaagaacatt ttacgtaccc agacactcct ctgcgacatg
     3901 ctcatgaggg acgctcccct cggcatcgtg agccagtccc ccaatattat ggatttagtc
     3961 aagtgcgacg gcgccgcctt attatacaag gacaagatct ggaagctggg caccacccct
     4021 agcgagttcc atttacaaga aatcgcttct tggctgtgtg agtaccacat ggattccacc
     4081 ggtttaagca ccgattcttt acacgacgct ggctttccta gggctttatc tttaggcgac
     4141 agcgtctgcg gaatggctgc cgttcgtatc agcagcaaag atatgatttt ctggtttcgt
     4201 tcccataccg ccggcgaagt gagatggggc ggcgccaaac acgaccccga tgatagggac
     4261 gatgccagaa ggatgcaccc cagatcctcc ttcaaggctt ttctggaggt ggtgaagacc
     4321 agaagcttac cttggaagga ctacgaaatg gatgccatcc actctttaca gctcatctta
     4381 cgtaacgcct ttaaggacag cgagaccacc gacgtgaaca caaaggtgat ctatagcaag
     4441 ctcaacgatc tcaagattga tggaatccaa tccggagggt ctggctccgg atcaagtggt
     4501 ggcagcggta ccggcgaggt gctgcacaag cccatcacca ccaagacagt ggaaggcgtg
     4561 ttctacagcg cctgggagac actggacctg ggcagacagg gcgtgaagcc tttcacagcc
     4621 cacagcgcca gagtgggagc cgctcaggac ctgctgaaga agggctacaa taccctgcag
     4681 atccagcagt ccggccggtg gtctagcgga gccatggtgg ccagatacgg cagagccatc
     4741 ctggctaggg atggcgctat ggcccacagc agagtgaaaa ccagatccgc ccccatgcag
     4801 tggggcaagg acgagaagga ccccaagaaa aagcggaagg tgtgagaatt cctgtgcctt
     4861 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4921 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4981 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5041 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5101 ggggctctag ggggtatccc cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg
     5161 gaaacaaacg cagaatccaa gcgcggccgc ccaggataca tagattacca caactccgag
     5221 cccttccacc gctagcgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     5281 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     5341 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     5401 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     5461 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     5521 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     5581 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     5641 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     5701 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     5761 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5941 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     6001 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6061 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6121 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     6181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     6301 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6961 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7441 tccagattta tcagcaataa accagcc
//
