LOCUS       pRO129-rtTA3            2370 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            rtTA3_yeast_moclo_part3_IDTyeastcodonoptimized.ape
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    8..11
                     /label="type_3_fw"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             9..719
                     /label="Translation 9-719"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDMLPGGS"
     misc_feature    12..713
                     /label="rtTA3_yeastcodonoptimized_IDT_from_addgeneplasmid60431"
                     /ApEinfo_revcolor="#9f7cff"
                     /ApEinfo_fwdcolor="#9f7cff"
     misc_feature    complement(716..719)
                     /label="type_3_rv"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(721..726)
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Antibiotic r... complement(729..837)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(838..1497)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(1498..1602)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Origin of Re... complement(1603..2366)
                     /label="ColE1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 GGTCTCATAT GAGTCGTTTA GATAAATCCA AGGTTATAAA CGGCGCACTA GAACTGCTTA
       61 ATGGCGTTGG TATCGAAGGT CTGACTACGA GGAAGTTAGC ACAGAAACTT GGGGTCGAGC
      121 AGCCCACGCT GTATTGGCAC GTAAAGAACA AGAGAGCGCT ATTAGATGCC CTACCAATAG
      181 AGATGTTAGA TAGACACCAT ACTCATTTTT GCCCGCTTGA GGGGGAAAGC TGGCAAGACT
      241 TTTTAAGAAA TAATGCAAAG AGTTACAGGT GCGCTCTTCT TTCACATAGG GATGGGGCAA
      301 AGGTACACTT AGGTACGAGG CCAACCGAGA AACAGTATGA AACACTAGAA AACCAACTTG
      361 CTTTTTTATG CCAACAGGGT TTCAGTCTTG AGAATGCACT TTATGCCCTT AGCGCAGTCG
      421 GGCACTTTAC GTTGGGTTGC GTCCTAGAGG AGCAGGAACA TCAAGTCGCA AAAGAGGAAC
      481 GTGAGACACC GACTACTGAT AGTATGCCCC CGTTATTACG TCAGGCAATA GAACTATTTG
      541 ACCGTCAAGG GGCGGAACCG GCTTTTCTTT TTGGCCTGGA GCTAATTATT TGTGGGCTGG
      601 AGAAGCAACT GAAGTGCGAA AGTGGTGGGC CAACTGACGC GCTAGATGAT TTCGATCTTG
      661 ATATGTTACC TGCAGATGCA CTAGATGACT TTGATTTAGA CATGTTGCCT GGAGGATCCT
      721 GAGACCagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      781 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      841 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      901 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      961 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1021 gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc
     1081 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1141 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1201 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1261 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     1321 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     1381 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     1441 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     1501 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1561 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt
     1621 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1681 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1741 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1801 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1861 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1921 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1981 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2041 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2101 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2161 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2221 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2281 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2341 tatcccctga ttctgtggat aaccgtagtc
//