LOCUS       pRO232-phlfO7           1906 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            mNeonGreen_in_yeast_moclo_vector.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    209..214
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     ytk             216..219
                     /label="t2 fw"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    222..251
                     /label="phlfO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    254..283
                     /label="phlfO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    286..315
                     /label="phlfO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    318..347
                     /label="phlfO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    350..379
                     /label="phlfO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    382..411
                     /label="phlfO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    414..443
                     /label="phlfO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(460..463)
                     /label="t2a rv"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(465..470)
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Antibiotic r... complement(473..581)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(582..1241)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(1242..1346)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1347..204)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
       61 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      181 tcccctgatt ctgtggataa ccgtagTCGG TCTCAaacgG GATGATACGA AACGTACCGT
      241 ATCGTTAAGG TGAATGATAC GAAACGTACC GTATCGTTAA GGTCAATGAT ACGAAACGTA
      301 CCGTATCGTT AAGGTCAATG ATACGAAACG TACCGTATCG TTAAGGTGTA TGATACGAAA
      361 CGTACCGTAT CGTTAAGGTA GATGATACGA AACGTACCGT ATCGTTAAGG TCGATGATAC
      421 GAAACGTACC GTATCGTTAA GGTCGGATCT CAGCTAGCTt tgaTGAGACC agaccaataa
      481 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      541 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      601 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      661 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      721 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      781 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
      841 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      901 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
      961 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1021 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1081 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1141 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1201 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1261 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1321 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     1381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1561 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagctt
//